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Southwest Research Extension Center Report of Progress 96 Kansas State University Agricultural Experiment Station and Cooperative Extension Service

K STATE Southwest Research-Extension Center efficacy of in-season applications of systemic insecticide to control Dectes stem borers in soybean by Larry Buschman, Holly Davis 2, and Phil Sloderbeck SUMMARY We tested six systemic insecticides applied to the soil and nine systemic insecticides applied to the foliage for their effectiveness in reducing Dectes stem borers (Dectes texanus) in soybean. The insecticides were applied during the beetle flight to target the first two instars of the insect developing inside the plants. Of the soil insecticides tested, only fipronil significantly reduced Dectes stem borer infestations with both applications. The late application of imidacloprid and acetamiprid also seemed to reduce Dectes stem borer infestations. Of the soil insecticides tested, only fipronil significantly reduced Dectes stem borer infestations with both applications. The late application of thiacloprid also seemed to reduce Dectes stem borer infestations. Dectes stem borer infestation was 20 to 25% of plants infested. PROCEDURES This trial was conducted in soybeans, Pioneer 93B85 (maturity group 3.8), planted May 20, 2005, on the Ramsey Brothers Farm 3 miles north of Garden City, Kansas. Three sets of plots were set up, one for soil-applied insecticides and two for foliar-applied insecticides. In two experiments, 20 treatments were assigned in a randomized complete-block design with five replications. In the third experiment, four treatments were assigned in a randomized completeblock design with five replications. Plots were four rows (0 ft) wide and 20 ft long, with a 5-ft alley across the ends of the plots. We tested six systemic insecticides applied to the soil and nine systemic insecticides applied to the foliage. The insecticides were applied during the beetle flight to target the first two instars of the insect developing inside the plants. The soil-applied treatments were applied July 20 and August 8, when the soybeans were 2 and 24 inches high, respectively. The liquid soil treatments were applied with a back-pack, hand-held sprayer with a single nozzle (fan LF3 80 o ) that was held close to the ground to apply a 6-inch band 6 inches from the base of the plants. The insecticides were incorporated by hand raking the soil and by irrigation several days later. The foliar treatments were applied July 22 and August 9 with the back pack sprayer, with a handheld boom with two nozzles (Conejet TXVS 6) directed at a single row. The nozzles were held 2 inches from the row and to each side. In both methods, the sprayer was calibrated to deliver 20 gal/acre (7.5 sec per 20 ft row at 30 psi). A chronometer was used to measure the time spent on each row to help maintain appropriate speed. Dectes stem borers infestations were recorded at the end of the season (September 5 to October 20) by dissecting 30 plants in each plot, taken from six locations in the two center rows. The plants were dissected to record any tunneling, tunneling that reached the base of the plant, and presence of live Dectes larvae. Grain yield data was not collected because infestations were very low, and the plants had been heavily damaged by hail. RESULTS AND DISCUSSION Dectes stem borer populations were much higher in 2005 than in 2004, but on July 4 there was a serious hail storm that seriously defoliated the soybeans. It also broke or bruised the stems. Although the plants recovered from buds, the resulting plants were smaller, later maturing, and more branched than normal. They were almost a month later than usual in reaching the stage at which Dectes beetles could oviposit in them. This meant that most of the plants escaped the main flight of Dectes beetles. A few of the plants were Department of Entomology, Kansas State University, Manhattan 65

tunneling in the stem, inasmuch as the number of larvae was much fewer than the number of tunnels in the stems. The second application was applied later than intended, due to an interruption from irrigation and rainy weather. In 2004, we were able to show a significant difference in yield (4.6 to 6.6 bu/acre) between the fipronil and the untreated check treatments. This implies a 7 to % physiological yield loss due to Dectes stem borer infestations. We did not take yield data in 2005. Fig. Dectes Stem Beetles in 00 sweeps in soybeans 2005 60 50 No. B eetles 40 30 20 st Applications 2nd Soil & Foliar Applications 0 0-0 0 0 20 30 40 50 60 Date from July Figure. Dectes stem beetles in 00 sweeps in soybeans, 2005. 66

Table. Efficacy of soil-applied systemic insecticides against Dectes stem borers in soybean. Southwest Research-Extension Center, Garden City, Kansas, 2005. R rates- tunneling Tunneling Larva Treat. Chemical product treatment time present to base present No. name Product name */000ft July 20 Both Aug 8 /30 plant /30 plants /30 plants 67. Check 6.3 ab 4.9 a 3.4 abc 2. Fipronil Regent 4SC 0.24oz 6.2 ml.2 d 0.4 d 0.3 d 3. Imidacloprid Provado.6 F.72 oz 44.3 ml 5.5 abc 3. abc 3.7 ab 4. Imidacloprid Provado.6 F.72 oz 44.3 ml 5.2 abc 3.0 abc 3.6 abc 5. Imidacloprid Provado.6 F 3.44 oz 88.6 ml 4.2 abc 2.4 abcd 2.5 abc 6. Thiamethoxam Platinum 0.46 oz.8 ml 6.8 a 3.5 abc 5.2 a 7. Thiamethoxam Platinum 0.46 oz.8 ml 4.4 abc 2.4 abcd 3.6 abc 8. Acetamiprid Intruder WSP 0.32 oz 3.26 gm 6.9 a 5. a 4.6 a 9. Acetamiprid Intruder WSP 0.32 oz 3.26 gm 6.9 a 3.3 abc 4.0 ab 0. Dinotefuran V-02 6.4 gm 4.28 6. ab 3.5 abc 4.0 ab. Dinotefuran V-02 6.4 gm 4.28 gm 6.3 ab 4. ab 4. ab 2. Acephate Orthene 90 S.0 oz 24.9 gm 5.9 abc 3.8 abc 4.4 a 3. Acephate Orthene 90 S.0 oz 24.9 gm 7.2 a 4. ab 5. a 4. Fipronil Regent 4SC 0.24 oz 6.2 ml 3.3 bcd.4 cd.3 cd 5. Imidacloprid Provado.6 F.72 oz 44.3 ml 2.7 cd.9 bcd.7 bcd 6. Imidacloprid Provado.6 F 3.44 oz 88.6 ml 5.6 abc 3.9 abc 3.8 ab 7. Thiamethoxam Platinum 0.46 oz.8 ml 5.7 abc 3.7 abc 4.6 a 8. Acetamiprid Intruder WSP 0.32 oz 3.26 gm 5.2 abc.6 bcd 3.9 ab 9 Dinotefuran V-02 6.4 gm 4.28 gm 4.4 abc 2. abcd 2.8 abc 20. Acephate Orthene 90 S.0 oz 24.9 gm 6. ab 2.8 abc 4.3 a F-test Prob. 0.0393 0.094 0.0009 CV 23 % 28 % 37 % Reference to specific products is provided solely for informational purposes. Experiments with pesticides on non-labeled crops or pests are part of the insecticide registration process; it does not imply endorsement or recommendation of non-labeled uses of pesticides by Kansas State University. All pesticide use must be consistent with current labels.

Table 2. Efficacy against the Dectes stem borers in soybean of systemic insecticides applied to foliage. Southwest Research-Extension Center, Garden City, Kansas, 2005. R rates- tunneling Tunneling Larva Treat. Chemical product treatment time present to base present No. name product name */acre July 22 Both Aug 9. /30 plant /30 plants /30 plants 68. Check 6.5 abcde 6.0 abc 5.0 ab 2. Fipronil Regent 4SC 0.3 lb ai/a 4.2 oz 6.2 ml. g.0 efg.0 ef 3. Thiacloprid Calypso 4F 0.25 lb ai/a 4 oz 5.9 ml 4.0 cdef 3.2 bcdef 2. def 4. Thiacloprid Calypso 4F 0.25 lb ai/a 4 oz 2X 3.4 defg 2.6 efg 2.5 bcdef 5. Clothianidin TM-4440 50WP.6 oz ai/a/coc 0.24 oz&% 4.4 gm 7. abc 6.3 ab 4.6 abc 6. Clothianidin TM-4440 50WP.6 oz ai/a/coc 0.24 oz&% 2X 4. cdef 3.9 abcde 2.8 bcde 7. Thiamethoxam Centric 40 WG 0.05 lb ai/a 2 oz 2.84 gm 8.6 a 7.2 a 6.0 a 8. Thiamethoxam Centric 40 WG 0.05 lb ai/a 2 oz 2X 3.6 bef 2.0 defg 2.4 cdef 9. Acetamiprid Intruder WSP 70% 2.3 oz 3.26 gm 4.8 bcdef 3.4 bcdef 2.9 bcde 0. Acetamiprid Intruder WSP 70% 2.3 oz 2X 3.8 def 3.0 cdefg 2.4 cdef. Dinotefuran V-02 70SG 0.76 lb ai/a 4.0 oz 5.7 gm 7.8 ab 5.4 abcde 3.4 abcd 2. Dinotefuran V-02 70SG 0.76 lb ai/a 4.0 oz 2X 6.3 abcde 5.0 abcde 3.5 abcd 3. Acephate Orthene 90S lb ai/a. lb 25 gm 7.4 abc 5.5 abcde 4.5 abcd 4 Fipronil Regent 4SC 0,3 lb ai/a 4.2 oz 6.2 ml 2.3 fg 0.7 g 0.7 f 5. Thiacloprid Calypso 4F 0.25 lb ai/a 4 oz 5.9 ml 5.6 abcde 5. abcde 4.7 abc 6. Clothianidin TM-4440 50WP.6 oz ai/a/coc 0.24 oz&% 4.4 gm 4.8 bcdef 3.6 bcde 2.4 cdef 7. Thiamethoxam Centric 40 WG 0.05 lb ai/a 2.0 oz 2.84 gm 6.8 abcd 6.0 abc 4.5 abcd 8. Acetamiprid Intruder WSP 70% 2.3 oz 3.26 gm 7.2 abc 5.8 abcd 4.6 abc 9 Dinotefuran V-02 70SG 0.76 lb ai/a 4.0 oz 5.7 gm 3.4 efg 3.2 bcdef 2.7 bcde 20. Acephate Orthene 90S lb ai/a. lb 25 gm 5.3 abcde 5.4 abcde 3.7 abcd F-test Prob. 0.0003 0.0005 0.0009 CV 22 % 24 % 23 % Reference to specific products is provided solely for informational purposes. Experiments with pesticides on non-labeled crops or pests are part of the insecticide registration process; it does not imply endorsement or recommendation of non-labeled uses of pesticides by Kansas State University. All pesticide use must be consistent with current labels.

Table 3. Efficacy against the Dectes stem borers in soybean of systemic insecticides applied to foliage August 9, Southwest Research-Extension, Center, Garden City, Kansas, 2005 R rates- Tunneling Tunneling Larva Treat. product present to base present No. Chemical name Product name */acre /30 plant /30 plants /30 plants. Check 6.2 5.2 4.2 2. Thiamethoxam & Centric 40 WG 2 oz Lambda cyhalothrin Warrior CS 3.8 oz 8.0 7.2 5.4 3. Lambda cyhalothrin Warrior CS 3.8 oz 8.2 7.2 5.0 4. Emamectin Benzoate Proclaim 5SG 4.8 oz 5.6 5.6 3.8 F-test Prob. 0.4662 0.6945 0.6957 CV 8 % 20 % 20% Reference to specific products is provided solely for informational purposes. Experiments with pesticides on non-labeled crops or pests are part of the insecticide registration process; it does not imply endorsement or recommendation of non-labeled uses of pesticides by Kansas State University. All pesticide use must be consistent with current labels. Dectes entry hole. Dectes tunnel with 3 entry holes. 69

Kansas State University Agricultural Experiment Station and Cooperative Extension Service, Manhattan 66506 SRP 96 August 2006 K-State Research and Extension is an equal opportunity provider and employer. 300