Control of Vine Mealybug, Planococcus ficus, in Wine Grapes using New Reduced-risk Insecticides in a Pest Management Program

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Control of Vine Mealybug, Planococcus ficus, in Wine Grapes using New Reduced-risk Insecticides in a Pest Management Program R. A. Van Steenwyk Dept. of E.S.P.M. University of California, Berkeley

Significant pest of grapes in North and South America, South Africa, Southern Europe, Middle East Discovered in California mid-1990s and rapidly spread Damage Vine Mealybug: Damage Produces copious amounts of honeydew sooty mold and infests grape bunches making them unfit for consumption Transmits grape leafroller virus complex

Vine Mealybug: Biology Overwinter as nymphs/adults under bark at or below graft union/roots 2 to 3 generations/year Coastal 5 to 7 generations/year San Joaquin Biological Control Anagyrus pseudococci Ant control Vine Mealybug: Biology and Control

Control of VMB in Grapes: Plot Design Pinot Grigio vineyard planted in 1990 Escalon, California 16 treatments replicated 6 to 8 times in RCB Each rep 3 vines (two outside vine considered treated buffer, not sampled) Moderate vine mealybug population

Control of VMB in Grapes: Application Methods Imidacloprid applied through a secondary drip cup under drip system at first irrigation All other treatments applied with hand-held spray system Spray volume 935 L/ha during spring increased to 1403 L/ha during summer

Control of VMB in Grapes: Evaluation Methods Ranked in 1 min search of the cordon or 5 fruit clusters per rep: Rank categories: 0: no VMB or honeydew 1: honeydew but no visible VMB 2: honeydew and visible VMB 3: honeydew and >10 VMB

Control of VMB in Grapes Trade Name Chemical Name Rate form/ha Timing 1) Applaud 70WP a Buprofezin 0.84 kg 13 Mar Movento 2SC + Spirotetramat 0.58 L Sivanto 1.67SL b Flupyradifurone 0.88 L 14 Jul 2) Applaud 70WP a Buprofezin 1.68 kg 13 Mar Movento 2SC + Spirotetramat 0.58 L Sivanto 1.67SL b Flupyradifurone 0.88 L 14 Jul 3) Lorsban Advanced a Chlorpyrifos 4.68 L 13 Mar Movento 2SC + Spirotetramat 0.58 L a Treatments included 0.023% V/V Latron B-1569 b Treatments included 0.0625% V/V Dyne-Amic 14 Jul Sequoia 2SC b Sulfoxaflor 0.42 L 4) Untreated check -- -- --

Control of VMB in Grapes 100 Buprofezin (low) / Spirotetramat + Flupyradifurone Chlorpyrifos / Spirotetramat + Sulfoxaflor Cordon Buprofezin (high) / Spirotetramat + Flupyradifurone Untreated check Fruit 2 Percent Control 75 50 25 1 Mean Rank VMB (Untreated Check) 0 6.3.15 6.16.15 6.30.15 7.13.15 7.28.15 8.10.15 0

Control of VMB in Grapes: Discussion Chlorpyrifos (standard - delay dormant treatment) provided superior control compared to buprofezin Chlorpyrifos provides secondary ant control No significant difference in low and high rates of buprofezin Addition of spirotetramat and sulfoxaflor to chlorpyrifos provided excellent season long control Addition of spirotetramat and flupyradifurone to buprofezin provided late season control

Control of VMB in Grapes Trade Name Chemical Name Rate form/ha Timing 1) Admire Pro Imidacloprid 1.02L 1 Apr Movento 2SC a Spirotetramat 0.58L 17 Jun 2) Admire Pro Imidacloprid 1.02L 1 Apr Movento 2SC + Spirotetramat 0.58L Assail 30SG a Acetamiprid 0.37kg 17 Jun 3) Admire Pro Imidacloprid 1.02L 1 Apr Movento 2SC a Spirotetramat 0.58L Sivanto 1.67SL + Flupyradifurone 0.88L 5 May Sequoia 2SC a Sulfoxaflor 0.42L 17 Jun 4) Untreated check -- -- -- a Treatments included 0.0625% V/V Dyne-Amic

Control of VMB in Grapes Imidacloprid / Spirotetramat Imidacloprid/Spirotetramat+Flupyradifurone/Sulfoxaflor 100 Cordon Imidacloprid / Spirotetramat + Acetamiprid Untreated check Fruit 2 Percent Control 75 50 25 1 Mean Rank VMB (Untreated Check) 0 6.3.15 6.16.15 6.30.15 7.13.15 7.28.15 8.10.15 0

Control of VMB in Grapes: Discussion Imidacloprid applied through the drip system provided marginal control Following an imidacloprid application the addition of acetamiprid to spirotetramat did not improve efficacy Following an imidacloprid application the addition of flupyradifurone to spirotetramat followed by sulfoxaflor did not improve efficacy as compared to imidacloprid followed by spirotetramat

Control of VMB in Grapes Trade Name Chemical Name Rate form/ha Timing 1) Sivanto 1.67SL a Flupyradifurone 0.88 L 5 May 17 June 2) Sivanto 1.67SL a Flupyradifurone 0.88 L 21 Apr Movento 2SC a Spirotetramat 0.58 L 17 Jun 3) Movento 2SC a Spirotetramat 0.58 L 5 May Sivanto 1.67SL a Flupyradifurone 0.88 L 17 Jun 4) Movento 2SC a Spirotetramat 0.46 L 5 May 17 June 5) Untreated check -- -- -- a Treatments included 0.0625% V/V Dyne-Amic b Treatments included 0.023% V/V Latron B-1569

Control of VMB in Grapes Flupyradifurone Spirotetramat / Flupyradifurone Flupyradifurone / Spirotetramat Spirotetramat 100 Cordon Fruit 2 Percent Control 75 50 25 1 Mean Rank VMB (Untreated Check) 0 6.3.15 6.16.15 6.30.15 7.13.15 7.28.15 8.10.15 0

Control of VMB in Grapes: Discussion Flupyradifurone followed by spirotetramat provided excellent control Spirotetramat followed by flupyradifurone provided excellent control Spirotetramat followed by spirotetramat provided excellent control Flupyradifurone followed by flupyradifurone provided marginal control

Control of VMB in Grapes Trade Name Chemical Name Rate form/ha Timing 1) Movento 2SC a Spirotetramat 0.46L 5 May Sequoia 2SC a Sulfoxaflor 0.42L 17 Jun 2) Sequoia 2SC a Sulfoxaflor 0.42L 5 May Movento 2SC a Spirotetramat 0.44L 17 Jun 3) Sequoia 2SC a Sulfoxaflor 0.42L 5 May 17 June 4) Untreated check -- -- -- a Treatments included 0.0625% V/V Dyne-Amic

Control of VMB in Grapes Spirotetramat / Sulfoxaflor Sulfoxaflor Sulfoxaflor / Spirotetramat Untreated check 100 Cordon Fruit 2 Percent Control 75 50 25 1 Mean Rank VMB (Untreated Check) 0 6.3.15 6.16.15 6.30.15 7.13.15 7.28.15 8.10.15 0

Control of VMB in Grapes: Discussion Sulfoxaflor followed by spirotetramat provided excellent control Spirotetramat followed by Sulfoxaflor provided excellent control Sulfoxaflor followed by Sulfoxaflor provided excellent control but control faded shortly before harvest

Control of VMB in Grapes: Conclusions All experimental materials provided effective control and fruit was harvestable Chlorpyrifos provided excellent control and is the standard for delayed dormant treatment Spirotetramat is backbones of in season control with best timing about two months prior to harvest Sulfoxaflor is a more effective material compared to flupyradifurone Effective programs against moderate VMB population would be: Chlorpyrifos at delayed dormant timing followed by spirotetramat combined with sulfoxaflor or flupyradifurone about 2 months prior to harvest

Acknowledgements I gratefully acknowledge the following pest control advisors: Charlie Starr and Jerry Moyer and growers: Lange Twins and Lone Creek vineyards whose assistance and cooperation made the above studies possible. I also acknowledge the invaluable contributions of Ruth Poliakon, Alyssa Hernandez and Ben Wong whose hard work resulted in the successful completion of this research and presentation.

QUESTIONS ANYONE?