NORTH CAROLINA TOBACCO 2000

NORTH CAROLINA MEASURED CROP PERFORMANCE TOBACCO 2000 D. T. Bowman A. Glenn Tart Official Variety Testing Department ofcrop Science North Carolina State University Raleigh, North Carolina 27695-8604

TABLE OF CONTENTS INTRODUCTION....... 1 EXPERIMENTAL PROCEDURES....... 2 RESULTS AND DISCUSSION....... 5 MAP 7 VARIETY DESCRIPTION.... 8 WEATHER GRAPHS........ 20 TABLES Table 1. Table 2. Table 3. Table 4. Cultural practices for the Official Variety Test, 2000........... 24 Comparison of certain varieties in Official Variety Trials across three years (1998-2000) 25 Comparison of certain varieties in Official Variety Trials across two years (1999-2000).. 26 Ratings for leaf ripening, holding ability, curability, and mechanical harvesting ability for selected commercial varieties.. 27 Table 5. Summary information on disease resistance, 2000 28 Table 6. Comparison of varieties for certain characteristics "for three locations, 2000............ 29 Table 7-9. Individual location data, 2000........ 30 Table 10-14. Comparison of advanced breeding lines - 2000... 33 Table 15. Pedigrees of entries in the 2000 Official Variety Test.... 38

INTRODUCTION l American flue-cured tobacco is known worldwide for its unique smoking qualities. The Flue-Cured Tobacco Minimum Standards Program was initiated in 1964 to ensure that released varieties have acceptable agronomic, physical, chemical and smoke characteristics. The North Carolina Official Variety Trials for flue-cured tobacco are conducted annually to provide information to growers and the tobacco industry on performance of varieties that have passed the Minimum Standards Program and may be available to the growers in North Carolina. Breeding lines are also included as a first step in advancing these potential varieties through the Minimum Standards Program. The Acreage-Poundage Program tends to encourage quality of flue-cured tobacco by limiting the production in terms of pounds per acre. varieties contribute substantially to leaf quality and should be carefully selected by the grower. Therefore, this bulletin has been written with emphasis on the quality of various varieties that are available to tobacco growers. Data are presented for three, two years and one year over all locations and by location in this bulletin. Growers are cautioned to examine data from two and three-year tables prior to selection of a variety to grow on their farms. They are also encouraged to grow a small acreage of the new variety rather than the whole crop at once. ITechnical assistance by Ken Barnes, John Denton, Phil Johnson, Dwight Parrish, and Jane Dove Long are gratefully acknowledged.

2 The Official Variety Trials consist of small replicated plots located on four research stations. The Regional Minimum Standards Program evaluates potential new varieties by the Regional Small Plot Test and Regional Farm Test. Results of these tests are reported separately in the Flue-Cured Variety Evaluation Committee Report. EXPERIMENTAL PROCEDURES Official variety Test Thirty-two released varieties and twenty-eight experimental lines were tested at four locations (Figure 1) in 2000. The Official Variety Tests were conducted on disease-free soil insofar as possible. The experimental locations are as follows: Border Belt Tobacco Research Station, Whiteville, NC, representing the Border Belt. Lower Coastal Plain Tobacco Research Station, Kinston, NC, representing the Eastern Belt. Upper Coastal Plain Research Station, Rocky Mount, NC, representing the Eastern Belt. Oxford Tobacco Research station, Oxford, NC, representing the Middle Belt.

3 Agencies, Contact Person, and Addresses of 2000 Sponsors Aqency and Contact Person Clemson University Albert Johnson Gold Leaf Seed Co. Marion Hawkins North Carolina State university Verne Sisson North Carolina State university Earl Wernsman Address Pee Dee REC Florence SC 29501 PO Box 458 Hartsville, SC 29551 PO Box 1168 Oxford, NC 27565 Crop Science Dept. North Carolina State University Raleigh, NC 27595-7620 varieties CU K, Coker, GL, X Oxford, OX, NC NC TG ProfiGen Mark Nielsen PROFIGEN A170 ASTECC Bldg Univ. of Kentucky Lexington, KY 40515 PV, PQ R. G. Seed Company Richard Gwynn speight Seed Farms Rachel Snyder VPI C.A. Wilkinson Route 6, Box 133 Oxford, NC 27565 Box 1250 Winterville, NC 28590 Southern Piedmont Research station Box 448 Blacksburg, VA 23824 RG Speight VA The entries were coded and seeded in plant beds which received normal cultural practices at three locations; greenhouse plants were used at all locations. At transplanting, the plants were individually selected for uniformity and planted into one-row plots, each of which consisted of twenty competitive plants spaced 22 or 24 inches apart, depending upon locations. The row spacing was four feet at all locations. Additional cultural practices are

4 shown in Table 1. Each entry was replicated three times in a randomized, complete block design at each location. 2 After topping, all entries were treated with commercial contact and systemic sucker control chemicals. Individual plots were harvested according to degree of maturity and primings were tagged and kept separate throughout curing, sorting, and grading. Performance data were collected on yield, quality, agronomic characteristics, disease resistance,3 and physical quality traits. Data on agronomic characteristics were collected in the field. Dollar value per hundredweight and grade indices are shown to emphasize quality differences among the varieties. Dollar value per hundredweight was calculated from a two-year average price paid per pound on the flue-cured market for a particular government grade. After the tobacco was sorted into lots, a Federal Tobacco Inspector assigned an appropriate government grade to each lot from each plot. The average dollar value per hundredweight. was then computed based on a weighted average by stalk position. Each entry was rated also with a grade index ranging from 1 to 100. This index was calculated by assigning a numerical value to each government grade of each entry. An average grade index value was then obtained for each entry in the same manner as dollar per hundredweight. Grades N2 and B1L represent the practical extremes with values of 1 and 100 assigned to these respective grades. The 2 statistical analyses were performed by Mrs. Joy smith. Her assistance is gratefully acknowledged. 3 Dr s. David Shew, and Tom Melton of the Plant Pathology Department and Dr. Verne Sisson of the Department of Crop Science cooperated on the tests for disease reaction. Their assistance is gratefully acknowledged.

5 2000 data utilized a revised version of the grade index as originally developed by E. Wernsman and E. Price (1975). SEASONAL CONDITIONS: Transplanting was somewhat delayed due primarily to slow growth of transplants (Table 1) which in turn was caused by the weather (data not shown). Below-normal rainfall was experienced at most locations in May and June; adequate rainfall was evident at most locations in July and August (see accompanying graphs). It continued to be dry in Oxford in July. As a consequence, Oxford applied 3.5 inches irrigation in JUly. Yield and quality was good to excellent depending on management. Overall it was an excellent year for tobacco production. Growers are cautioned against using anyone-year or one location data in making varietal selection decisions. RESULTS AND DISCUSSION The data presented in Tables 2 and 3 summarize how varieties performed over a period of years at various locations. These tables give a general indication of the stability of the varieties since they include performance data over many locations and years. In Table 2, varieties that were common in 1998, 1999, and 2000 are compared for a number of agronomic characteristics. Two-year data are presented in Table 3 for 1999 and 2000. Table 4 shows ratings for leaf ripening rate, holding ability, curability, and machine harvesting ability; these ratings are based on data collected over years and locations thus some of the newest varieties may not have ratings for leaf ripening rate and holding ability.

6 Information on disease resistance is presented in Table 5. Data were collected on black shank, bacterial wilt, root knot nematodes, and mosaic. Root knot resistance is recorded as resistant or segregating; virus resistance is identified by type. These data were furnished by Drs. Tom Melton, Verne Sisson, and David Shew. The average performance across three locations in 2000 is shown in Table 6. The Whiteville location was discarded due to drowning early in the season. Individual location data for commercial varieties are presented in Tables 7-9 while advanced breeding line data are found in Tables 10-14. Table 15 lists the pedigrees, year of release, and the developer of commercial varieties and advanced breeding lines in the 2000 Official Tobacco Variety Tests.

FIGURE 1- LOCATION OF OFFICIAL VARIETY TEST NORTH CAROLINA AGRICULTURAL RESEARCH SERVICE ~ T Experiment Station-Tobacco

8 VARIETY DESCRIPTION Information regarding agronomic performance which mayor may not be found in this and other publications for the commercially available varieties is listed for the grower's benefit. Coker 48 - It has high resistance to black shank and Granville wilt. It yields better than the check varieties, NC 95 and NC 2326. Days to flower after transplanting is about average. Average leaf number is nearly 20 leaves per plant on a higher than average stalk. Coker 176 - This variety has low resistance to black shank and Granville wilt, and resistance to Fusarium wilt, root knot nematodes, and tobacco mosaic virus. This variety has a moderate number of leaves on a medium height stalk. It has tolerance to weather fleck. It has fair-good 'holding ability.' Coker 319 - It has low resistance to black shank and Granville wilt. It yields comparable to the standard varieties and has a much higher quality as indicated by grade index. Coker 371 Gold - It was developed by Coker's Pedigreed Seed Company from a complex cross involving Speight G-28 and NC 82. Coker 371 Gold has high resistance to black shank and moderate resistance to Granville wilt and brown spot. It has fair-good 'holding ability.' CU 263 - This variety was developed by Clemson University with tobacco budworm resistance on a Speight G-28 background. It ripens slowly on the stalk and is more adapted to the Border and Eastern Belts. It produces good yields width better than average quality. It has tall plants with an average of 20 leaves per plant. It is moderately easy to cure.

9 GL939 - This variety has high resistance to both black shank and Granville wilt with additional resistance to root-knot nematodes. It has K 326 as one of its parents. It has excellent curability with a late leaf-ripening rate. It has good mechanical harvesting ability with '19 leaves on a 37 inch stalk. It averages 65 days from transplanting to mid-bloom. It has average yields of mostly medium bodied orange tobacco. X-149 This variety was developed from a number of crosses involving Speight G-28, K 399 and several breeding lines. It has moderate resistance to black shank and high resistance to Granville wilt and is resistant to the Southern root-knot nematode. It has fair-good 'holding ability.' X 326 - It has low resistance to black shank and Granville wilt; it has resistance to the common root-knot nematodes. It is known for its high quality and curability. It has a moderate number of leaves on a ~ow stalk. It is tolerant to brown spot and has excellent 'holding ability.' X 340 - (Tested as NK 3240) was developed by Northrup King Seed Company from a cross of McNair 944 and NC 82. K 340 has high resistance to black shank and moderate resistance to Granville wilt. It has a low ground sucker count and flowers, on the average, 64-72 days after transplanting with nearly 19 harvestable leaves. X-346 Developed by Northrup King Seed Company from a cross involving K 326 and a breeding line. It has high resistance to black shank and Granville wilt and is resistant to the Southern root-knot nematode. It has good-excellent 'holding ability.'

10 X 358 - (Tested as NK 5168) was developed by Northrup King Seed Company from a cross of K 326 and 80241 (an experimental line). It has moderate resistance to black shank and Granville wilt. It is also resistant to root-knot nematodes. It has above average yields with 18-19 leaves on a medium height stalk. It has few ground suckers and excellent 'holding ability.' X 394 - It has high resistance to black shank and low resistance to Granville wilt. It averages nearly 20 leaves per plant on a short stalk. It averages 66-72 days to flower. It is a high-yielding variety with average quality but with only fair 'holding ability.' X 399 - This variety carries high resistance to black shank and Granville wilt. It is resistant to the most prevalent species of root-knot nematodes occurring in the flue-cured tobacco area. It is sensitive to weather fleck. It has fair-good 'holding ability.' X 730 - This variety developed from a cross between K 326 and the breeding line 80241. K 730 has low resistance to black shank and high resistance to Granville wilt. It produces good yields of good quality tobacco with good 'holding ability.' It has resistance to Southern root-knot nematodes. It averages 19 leaves on a medium height stalk. McNair 373 - This variety has a number of leaves with a short stalk. It has low resistance to black shank, moderate resistance to Granville wilt and resistance to root-knot nematodes. A short, compact plant of good storm resistance, it tends to mature at a moderate rate and flowers somewhat earlier than some multi-disease resistance varieties. leaf. It produces adequate yields of high-quality

11 McNair 944 - It has moderate resistance to black shank and low resistance to Granville wilt. It is a high-yielding variety with average quality. NC 27NF - This is a "non-flowering" variety that produces high yields with a high grade index. It was developed by breeders at North Carolina state University from a cross of Coker 319 and a non-flowering genotype. NC 27NF has low resistance to black shank and Granville wilt. It is sensitive to brown spot and has good 'holding ability.' NC 37NP - This is a "non-flowering" variety that produces high quality leaf. It was developed by breeders at North Carolina state University from cross involving a non-flowering genotype with Coker 319 and NC 82. It has low resistance to black shank and Granville wilt and resistance to root-knot nematodes and has only fair 'holding ability.' NC 55 - Developed from a cross of K 326 and Coker 371 Gold with low resistance to black shank and Granville wilt. It also has resistance to Southern root know nematodes and potato virus 'y' or PVY. It yields nearly the same as K 326 with equal quality and curability. Its' days to flower, plant height, and leaf number are similar to K 326. NC 60 - This variety produces good yields with a high grade index. It was developed at the Oxford Tobacco research station from a cross between McNair 944 and Speight G-28. This variety has high resistance to black shank and moderate resistance to Granville wilt and is resistant to the common root knot nematode. NC 60 has above

12 average number of ground suckers and produces slightly more than 19 leaves on the average. NC 71 Hybrid flue-cured tobacco with high black shank and moderate Granville wilt resistance and also resistant to Southern root-knot nematode. Yield, quality, and curability are the same as K 326. Days to flower, plant height, and number of leaves per plant are similar to K 326. NC 72 - This is an F1 hybrid with excellent resistance to black shank and root-knot nematodes and low resistance to Granville wilt. It produces high yields on a 39 inch stalk averaging 18.6 leaves. It is a full-season variety (late leaf ripening) with excellent curability and good mechanical harvest ability. Leaves are mostly medium-bodied lemon and orange. NC 82 - An average yielding variety with high quality. It has an intermediate number of leaves on a medium to short stalk. Flowers fairly early and may prematurely flower. It has high resistance to black shank and moderate resistance to Granville wilt, and brown spot. It is not subject to extensive damage from weather fleck and good 'holding ability.' NC 95 - One of the first varieties with disease resistance and high quality. It has low resistance to black shank and high resistance to Granville wilt and resistance to the most prevalent species of root knot nematodes found in North Carolina and to Fusarium wilt. NC 297 - A high-yielding hybrid with average quality but with resistance to Tobacco Mosaic Virus. It has very high resistance to Black Shank and moderate resistance to Granville wilt. It is also resistant to the most prevalent species of root-knot nematodes

13 found in North Carolina. It averages nearly 19 leaves on a short plant, with fair harvest ability. It has a with fair holding ability. late leaf ripening rate NC 606 - An average-yielding variety with excellent curability. It averages nearly 19 leaves on an average plant height. It has moderate resistance to Black Shank, Granville wilt, and is resistant to the most prevalent species of root-knot nematodes. It has a late leaf ripening rate. NC 729 - This variety has high resistance to Granville wilt and low resistance to black shank. It produces 19-20 leaves on a short stalk with fair-good 'holding ability.' NC 2326 - This variety has low levels of resistance to black shank and is susceptible to Granville wilt. It is essentially a Hickstype tobacco with is known for its unique quality in terms of flavor and aroma of the cured leaf. It is used as one of the standard varieties. IT has a tendency to flower prematurely. Oxford 207 - This variety has high resistance to black shank and high resistance to Granville wilt with additional resistance to root-knot nematodes. It produces average yields with nearly 19 leaves on a 37" stalk. It has fair mechanical harvest ability (leaves are fairly close together). Oxford 207 has good curability and averages 69 days from transplanting to mid-bloom. Leaves are medium bodied with primarily orange color. Oxford 414NF - This variety, developed by N. C. State University, is a cross between NC 37 NF and K 346. It has moderate black shank and Granville wilt resistance. It is tolerant to the Southern

14 root-knot nematode. It produces excellent yields with excellent curability (ease of curing). It is a non flowering variety. Oxford 940 - It resulted from a cross between Speight G-28 and Coker 347 - It has excellent resistance to black shank and moderate resistance to Granville wilt. It produces slightly below average yields of average quality tobacco. Leaves average nearly 20 per plant on a short stalk. Leaves ripen early and it has only fair 'holding ability.' PVH03 - This is a hybrid developed by Profigen with low resistance to black shank and moderate resistance to Granville wilt. It is resistant to both Southern root-knot nematode and tobacco mosaic virus. It produces moderate y~elds of good quality tobacco. PVH09 - This is a hybrid developed by Profigen with low resistance to black shank and moderate,resistance to Granville wilt. It is also resistant to the Southern root-knot nematode and tobacco mosaic virus. It produces moderate yields of fair quality tobacco. Reams Hl - This resulted from a cross of Speight G-28 and Reams 158. It has moderate resistance to both black shank and Gravnille wilt. It produces slightly below average yields of average quality tobacco. Days to flower, plant height, and leaf number are average compared to other varieties. Leaves ripen average, i.e. not early or late and it has fair-good 'holding ability.' Reams 134 - This variety produces high quality tobacco on a medium high stalk. It was developed by Reams Seed Company from a cross between McNair 944 and Hicks. 'It yields in the low range with 20 leaves per stalk. It has moderate resistance to black shank with

15 low resistance to Granville wilt. It has fair-good 'holding ability'. Reams 158 - This variety produces moderately low yields with a high grade index.. It was developed by Reams Seed Company from a cross between McNair 944 and Hicks. This variety has moderate resistance to black shank and low resistance to Granville wilt. It produces 19-20 leaves on a slightly higher than average plant. RG8 - Has moderate resistance to Granville wilt and black shank. It produces nearly 20 leaves on a short stalk. It has excellent 'holding ability.' RGll - This variety has high resistance to both black shank and Granville wilt and has resistance to the most prevalent species of root-knot nematodes. It produces average yields on a tall stalk with 21 leaves per stalk. The cured leaf has excellent physical quality. There isn't a problem with premature flowering in a normal year. It has good-excellent 'holding ability.' RG13 - It has moderate resistance to both black shank and Granville wilt and resistant to the most prevalent species of root-knot nematodes. It produces average yields and quality and has fairgood 'holding ability.' RG17 - It has high resistance to Granville wilt and is resistant to the Southern root-knot nematode. It has tolerance to brown spot but low resistance to black shank. It produces high yields of good quality tobacco. It is fairly easy to cure with nearly 19 leaves on a medium height stalk. Leaves ripen at a moderate rate and it has excellent.'holding ability.'

16 RG22 Has high resistance to Granville wilt and moderate resistance to black shank. It produces 19-20 leaves on a short stalk. It has good-excellent 'holding ability.' RG81 - Developed from a cross of K 326 and K 399. It yields nearly the same as K 326 with slightly lower quality. It is moderately easy to cure. Days to flower, plant height, and number of leaves per plant are similar to K 326. It is a late maturing- variety with moderate resistance to black shank and Granville wilt and also resistant to Southern root knot nematode. RGH4 Hybrid flue-cured tobacco with tobacco mosaic virus resistance. It also has moderate resistance to black shank and high resistance to Granville wilt with resistance to Southern rootknot nematode. It has slightly lower yield and quality than K 326. It is moderately easy to cure. It is slightly taller than K 326 with leaves spaced farther apart than K 326. It has a moderate leaf ripening rate. RGH12 - Hybrid flue-cured tobacco with moderate resistance to black shank and Granville wilt along with resistance to root-knot nematodes. It is an early variety in terms of leaf ripening rate with excellent curability. It yields above average. RGHSl - This is a hybrid developed by RG Seeds with high resistance to black shank and moderate resistance to Granville wilt. It is also resistant to the Southern root-knot nematode. It produces excellent yields of excellent quality tobacco. RGH61 - Hybrid flue-cured tobacco. It has moderate resistance to black shank and low resistance to Granville wilt and resistance to Southern root-knot nematodes. It yields slightly less than K 326

17 with equal quality and ease of curing. It flowers four days earlier than K 326. speight G-28 It has moderate resistance to black shank and Granville wilt with resistance to Fusarium wilt and root-knot nematodes. It averages around 66 days from transplanting to flowering with more than 19 leaves on a short stalk. This variety has very few ground suckers. It has good 'holding ability.' speight G-70 - This variety yields much higher than NC 2326 and quality is about the same. It has an intermediate number of leaves with a low stalk, flowers about average. It has resistance to root knot nematodes. It has high resistance to black shank and low resistance to Granville wilt. Its cured leaf is about the same color and texture as NC 2326 with a higher percentage of medium to heavy bodied tobacco, less chaffy leaf and lower nicotine content. speight G-l08 It has moderate resistance to black shank and Granville wilt and is also resistant to the common root-knot nematode. It was developed by Speight Seed Farms from a cross of Speight G-70 and Speight G-28. This variety flowers on the average 70 days after transplanting with 19 harvestable leaves. Speight G-lll - This variety has moderate resistance to both black shank and Granville wilt and has resistance to the most prevalent species of root-knot nematodes. It produces above-average quality leaves at an average yield per acre. It has excellent 'holding ability. ' Speight G-126 - This variety came from a cross between K 326 and Speight G-96. Granville wilt. It has moderate resistance to both black shanks and This variety produces average yields of less than

18 average quality. It produces nearly 20 leaves on a stalk of average height and flowers later than most varieties. 'holding ability.' It has good Speight 168 It has excellent resistance to black shank and Granville wilt and root-knot nematodes. It produces average yields on a 3 foot stalk with good leaf spacing. Leaves ripen at a moderate rate with excellent curability and mostly lemon and orange color with medium body. Speight 172 - This variety has high resistance to black shank and moderate resistance to Granville wilt and is resistant to root-knot nematodes. It produces excellent yields on a moderate-size stalk. Leaves ripen at a moderate rate with good curability and primarily orange in color and medium body. Leaves are spaced apart at an above-average distance. speight 179 - The variety resulted from a cross of Coker 371 Gold and Speight G-28. It has high resistance to black shank and Granville wilt. It is also resistant to Southern root-knot nematodes. It produces moderate yields of good quality tobacco. Speight H20 - An average-yielding variety with average curability. It produces 19 harvestable leaves on 42 inch stalk which provides one of the widest leaf spacings for machine harvest. It has very high black shank resistance, high Granville wilt resistance; resistance to tobacco mosaic virus and the most prevalent species of root-knot nematodes. It has a moderate to late leaf ripening rate. speight NF3 - This is a non-flowering variety with six genetic sources in its pedigree. It has high resistance to both black

19 shank and Granville wilt. It produces average yields on a moderate-size stalk. Leaves ripen at a moderate rate with good curability and mostly orange in color with medium body and texture. The leaves are spaced apart at a moderate distance. VA 116 This variety was developed by Virginia Polytechnic Institute and Southern University from a cross involving NC 82 and Coker 319. It has moderate resistance to black shank and low resistance to Granville wilt. It has only fair 'holding ability.'

eatherlimbus.co. Weekly Weather Data ~O) (April - August 2000) RainfallI. 28 4 11 18 25 31 August ~ 120 ~ ~ 100 ( - ~ 80 ~ ": - ": -,.. 60 '",.. 40 '" - ] ] 20 I... Max Temp...- Min Temp o Rainfall I ( C 0'"''. ",,,,." II ",,,,",,,,,,,,. -, Ii, " <I I. '{- II, [. "-"'-!'" \~, II -" ~ "", -,", )",, " I ' 7 142128 5121926 29162330 7 142128 4 11182531 April May June July August 6.5 6 5.5 5 4.5 :0 Ol 4 S --ft Ol I\J 3.5 0 ~ 3 ::J 0 2.5 ::Y CD CJ) 2 ---- 1.5 1 0.5 0

00) y WeatClgecombe Co. Weekly Weather IRainfall I 100 -- Data (April - August 2000) 1--Max Temp... Min Temp o RainfallI I 60 80 -.- 40 _. :D ~. - 3 ::J ~ 2.5.-=::: ::J o - 2 zr CD (J) '-"'" 1.5 I\) OJ ~ 1 28 4 11 18 25 31 August ( 20 - o ',. II II I "" ', " " 11 11.-', - 11 '11 '"' "', " e I' 11 ''1 " "'I"" ' 7 142128 5 121926 2 9 162330 7 142128 4 11182531 Ap ril May June July August 1-0.5 0

eatheillville Co. Weeldy Weather Data 000) (May-September 2000) grainfalli 120 I " 5 1--Max Temp "* Min Temp EJRainfalll 100.' - 4 80 -I r I I :D ~. 3 :::J -h I\.) 0) I\.) 60 -I r I /,:,I~~ I --:::::: :::J o 2 zr 40--1 1 :1 I I F\i~ ; 1 I I _I CD (J) ""'--'" 20. 14 21 28 4. 11 18 25 31.ugust September. o, I I..Y~" II ';': '1,.. ~ ".:'. ", ~II ' I I '> II' :C' ~-", ;1-' II '" II 1 I"'''":;...,, I h~" 1 V '''-.-11 :,~,.;!! <_." ~". 0 7 14 21 28 5 12 19 26 3 10 17 24 31 7 14 21 28 4 11 18 25 31 May June July August September

eather rloir Co. Weekly Weather Data 00) (April - August 2000) ainfall I 1 120 I 1--Max Temp SFMin Temp DRainfall I I 3.5 100 I 2.5 801~ I <I :n 60 I A / ~-- ~rll... n --:::::: ::::J 1.5 o zr 'co CJ) 40 I~I II-I '-" I I 11 I I I I I II 1 20 I I II II I II lin 1111 nlllill l ll l 0.5 3 2 ~. ::::J s:u -h ~ w \ 4 21 28 4 11 18 25 31 July August o I II II I I I I I " II " " I I " " " I I " II " " II 0 7 14 2128 5 12 19 26 2 9 16 23 30 7 14 2128 4 11 18 25 31 April May June July August

TABLE 1. CULTURAL PRACTICES FOR THE OFFICIAL VARIETY TEST - 2000. Chemical Date Date Fertili- Side- Soil Soil of First Station zation Dressing Type Treatment Transplanting Harvest Border Belt Tobacco 700lA 200#/A Norfolk Fine Telone C-17 19 Apr 26 July Research station 6-12-18 15-0-14 Sandy Loam 12 galla Whiteville, NC Ridomil 1 pt/a Lower Coastal Plain 475#/A 130#/A Goldsboro Ridomil 1 qt/a 25 Apr Research station 8-8-24 15-0-14 Fine Telone C-17 Kinston, NC Sandy Loam 10 galla Lorsban 2 qt/a Upper Coastal Plain 400#/A 200#/A Norfolk Nemacure 2gai/A 1 May Research station 8-8-24 16-0-0 Sandy Loam Lorsban 2qt/A Rocky Mount, NC Oxford Tobacco 5001/A 150#/A Helena uradan 4F 1 gall 10 May 2 Aug Research Station 8-8-24 15-0-14 Sandy Loam hlor-o-pic 6 galla ~ Oxford, NC Ridomil 1 pt/a ~

25 TABLE 2. COMPARISON OF COMMERCIAL VARIETIES IN OFFICIAL VARIETY TRIALS ACROSS THREE YEARS (.1998-2000). CURED LEAF ANAI.xSIS DAYS LEAVES PLANT NODE RED. TOT. RATIO YIELD VALUE INDEX GRADE TO PER HEIGHT LENGTH SUG. ALK. SUG. VARIETY LBS/A $/A $/CWT. INDEX FLOWER PLANT INCHES INCHES,, ALK. COKER 371-GOLD 2584 4436 171.22 75 67 18.7 38 2.04 15.67 3.26 5.24 GL 939 2713 4667 171.69 73 68 20.1 39 1.94 16.33 3.00 5.97 K 149 2786 4787 171.53 73 70 20.1 40 2.01 15.99 2.62 6.87 K 326 2943 5061 171.67 73 72 20.0 40 2.01 16.54 2.61 6.98 K 346 2807 4825 171.61 72 69 19.8 40 2.05 16.41 2.80 6.43 K 358 2693 4622 171.36 73 69 19.3 40 2.07 15.63 2.76 6.30 K 394 3025 5147 169.76 68 72 19.6 40 2.05 18.00 2.45 8.24 K 399 2860 4898 170.86 70 69 19.7 39 1.98 15.94 2.71 6.62 K 730 2791 4813 172.31 75 69 19.4 40 2.04 16.46 2.79 6.50 NC 55 3038 5264 173.19 76 71 19.8 39 1.95 15.87 2.80 6.09 NC 71 3140 5427 172.65 75 71 19.8 39 1.98 16.80 2.91 6.38 NC 72 3026 5224 172.54 74 71 19.2 42 2.18 16.47 2.62 6.73 OX 207 2676 4564 170.12 70 72 19.5 39 2.03 15.28 2.90 5.80 RG 17 2920 5040 172.45 75 70 19.7 40 2.01 16.25 2.68 6.61 RG 81 2944 5051 171.40 71 71 19.1 39 2.07 16.23 2.63 6.70 RGH12 2784 4763 170.73 73 70 19.4 40 2.05 15.99 2.85 6.36 RGH4 2915 4969 170.49 68 68 19.7 43 2.19 15.79 3.09 5.76 RGH61 2883 4958 171.53 73 69 19.0 41 2.16 16.72 2.77 6.77 SPEIGHT 168 2818 4858 171.86 73 71 18.9 39 2.08 16.02 2.87 6.21 SPEIGHT 179 2878 4954 171.91 72 72 19.8 40 2.03 14.85 3.02 5.33 SPEIGHT NF 3 2742 4723 172.17 74 72 19.8 41 2.05 14.96 2.75 6.02

26 TABLE 3. COMPARISON OF COMMERCIAL VARIETIES IN OFFICIAL VARIETY TRIALS ACROSS TWO YEARS (1999-2000). CURED I.EAF ANAI,YSIS DAYS LEAVES PLANT NODE RED. TOT. RATIO YIELD VALUE INDEX GRADE TO PER HEIGHT LENGTH SUG. ALK. SUG. VARIETY LBS/A $/A $/CWT. INDEX FLOWER PLANT INCHES INCHES,, ALK. COKER 371-GOLD 2476 4238 170.72 75 69 18.5 38 2.07 15.48 3.25 5.29 GL 939 2638 4519 170.96 73 68 19.6 39 1.98 16.15 3.04 5.95 K 149 2662 4545 170.55 71 70 20.0 40 2.03 15.70 2.65 6.84 K 326 2851 4882 170.96 73 71 19.5 40 2.05 16.63 2.58 7.16 K 346 2731 4677 171.00 71 69 19.7 41 2.08 16.29 2.79 6.55 K 358 2636 4504 170.62 72 69 19.0 40 2.09 15.25 2.73 6.29 K 394 2928 4962 169.09 68 72 19.4 40 2.09 18.03 2.37 8.67 K 399 2766 4708 169.92 68 68 19.8 39 1.99 15.85 2.61 6.90 K 730 2701 4636 171.62 74 69 19.3 40 2.07 16.93 2.76 6.83 NC 55 3011 5198 172.63 75 70 19.9 39 1.97 16.09 2.83 6.22 NC 606 2718 4691 172.42 78 70 18.9 41 2.16 17.76 3.09 6.55 NC 71 3096 5333 172.06 74 71 19.4 39 2.00 16.83 2.87 6.51 NC 72 2988 5133 171.80 72 71 19.1 42 2.20 16.35 2.65 6.70 OX 207 2631 4465 169.27 69 72 19.0 39 2.08 14.84 2.97 5.63 OX 414 NF 2937 5092 173.28 77 73 20.5 41 2.02 15.08 2.88 6.05 RG 17 2824 4859 171.96 75 70 19.3 39 2.05 16.16 2.68 6.69 RG 81 2902 4958 170.69 71 69 19.2 40 2.10 16.21 2.59 6.91 RGH12 2705 4607 169.99 72 70 19.1 40 2.08 15.66 2.82 6.46 RGH4 2837 4817 169.85 68 69 19.4 43 2.22 15.76 3.10 5.88 RGH51 2906 4984 171.24 73 70 19.1 41 2.17 16.96 2.74 6.67 RGH61 2793 4783 170.78 72 69 18.8 42 2.21 16.75 2.74 7.00 SPEIGHT 168 2715 4667 171.37 74 70 19.0 40 2.10 15.94 2.89 6.26 SPEIGHT 179 2822 4850 171.59 72 72 19.7 40 2.03 14.53 3.13 5.07 SPEIGHT 190 2807 4786 170.29 71 72 19.8 41 2.08 16.55 2.81 6.35 SPEIGHT NF 3 2691 4616 171.48 72 72 19.5 41 2.07 14.92 2.77 6.08

27 TABLE 4. RATINGS FOR LEAF RIPENING, HOLDING ABILITY, CURABILITY AND MECHANICAL HARVESTING ABILITY FOR SELECTED COMMERCIAL VARIETIES. LEAF HOLDING HARVEST VARIETY RIPENING ABILITY CURABILIITY ABILITY COKER 371 Early/Mod Good/Fair Exc. Fair GL 737 Mod.//Late NA Good Good GL 939 Late NA Exc. Good K 149 Late Good/Fair Exc. Fair K 326 Late Exc. Exc. Fair K 346 Late Exc./Good Good Good K 358 Mod./Late Exc. Exc. Good K 394 Mod./Late Fair Fair Good K 399 Mod./Late Good/Fair Good Fair K 730 Moderate Good Exc. Good NC 55 Mod./Late NA Exc. Fair NC 71 Late Exc. Exc. Fair NC 72 Mod./Late Exc. Exc. Good NC 297 Late Fair Good Fair OX 207 Mod./Late NA Good Good OX 414 NF Mod./Late NA Exc. Fair NC 606 Late NA Exc. Good RG 17 Mod./Late Exc./Good Exc. Fair RG 81 Mod./Late Good Exc. Fair RGH4 Mod./Late NA Exc. Good RGH12 Early NA Exc. Fair RGH51 Mod./Late Exc./Good Exc. Exc. RGH61 Mod./Late Exc./Good Exc. Good RG 81 Mod./Late NA Good Fair SPEIGHT 168 Mod./Late Exc./Good Exc. Good SPEIGHT 172 Mod./Late NA Good Good SPEIGHT 179 Late Exc./Good Good Fair SPEIGHT 190 Mod./Late NA Exc. Fair SPEIGHT NF3 Mod./Late Exc./Good Exc. Good SPEIGHT H2O Mod./Late NA Exc. Good 'NA=Data not available.

28 TABLE 5. SUMMARY INFORMATION ON DISEASE RESISTANCE - 2000. VARIETY BLACK ~ GRANVILLE ~ ROOT-KNOT NEMATODE 1 Coker 371 9 42 GL 939 29 30 R K 149 35 25 R K 326 52 48 R K 346 18 32 R K 358 37 32 R K 394 20 56 K 399 19 35 R K 730 48 39 R NC 55 59 52 R PVY, Etch NC 71 9 45 R NC 72 5 51 R NC 297 6 41 R TMV NC 606 18 30 R OX 207 19 23 R OX 414 NF 21 44 R RG 17 37 31 R RG 81 50 46 R RGH4 28 31 R TMV RGH12 30 43 R RGH51 15 45 R RGH61 36 46 R Speight NF3 17 33 R Speight 168 3 23 R Speight 190 9 26 R Speight 179 2 28 R Speight H20 7 32 R TMV 1varieties are rated with numerical ratings; the lower the number the higher the resistance. Root-knot nematode resistance is rated either resistant or blank (susceptible). 2pvy - Potato virus 'y', TMV - Tobacco Mosaic Virus

29 TABLE 6. COMPARISON OF VARIETIES FOR CERTAIN CHARACTERISTICS FOR THREE LOCATIONS - 2000. CURED LEAF ANALYSIS DAYS LEAVES NODE RED. TOT. RATIO YIELD VALUE INDEX GRADE TO PER PLANT SPACE SUG. ALK. SUG. VARIETY LBS/A $/A $/CWT. INDEX FLOWER PLANT HEIGHT INCHES % % ALK. COKER 371-GOLD 2150 3689 171.21 75 69 17.7 37 2.11 19.23 2.95 7.06 GL 939 2406 4122 171.21 73 70 19.6 38 1.92 19.56 2.78 7.51 K 149 2270 3904 171.77 72 69 19.1 40 2.09 19.39 2.49 8.91 K 326 2639 4543 172.01 73 71 18.8 38 2.04 19.42 2.31 9.25 K 346 2534 4372 172.35 72 67 20.0 41 2.04 20.63 2.51 8.50 K 358 2404 4168 173.06 77 71 19.1 39 2.05 19.21 2.54 8.58 K 394 2438 4112 168.90 68 71 18.8 39 2.08 23.27 2.08 12.27 K 399 2464 4202 170.05 67 68 19.4 38 1.94 19.13 2.43 8.92 K 730 2403 4134 172.15 74 69 19.0 38 2.02 20.38 2.44 9.04 NC 55 2743 4792 174.85 79 69 19.1 38 1.98 20.12 2.59 8.19 NC 71 2822 4876 172.64 72 70 18.3 37 2.03 19.81 2.57 8.39 NC 72 2706 4706 173.68 74 71 18.8 41 2.18 19.71 2.44 8.63 NC 297 2704 4668 171.60 68 68 18.8 39 2.07 20.86 2.37 9.36 NC 606 2377 4144 174.19 81 69 18.2 39 2.16 21.62 2.56 9.20 OX 207 2181 3701 169.25 68 72 18.6 37 1.98 18.96 2.79 7.38 OX 414 NF 2528 4444 175.37 82 73 20.4 42 2.05 19.29 2.41 8.86 RGH4 2533 4384 172.68 71 68 19.3 42 2.18 18.77 2.89 7.63 RGH12 2264 3842 169.10 70 70 19.4 38 1.97 20.08 2.46 8.84 RG 17 2508 4325 172.35 74 70 18.7 39 2.07 19.78 2.46 8.77 RGH51 2449 4212 171.19 70 71 19.1 39 2.07 20.33 2.47 8.60 RGH61 2401 4122 170.75 70 68 18.8 40 2.13 19.86 2.45 8.80 RG 81 2611 4500 172.06 69 69 18.9 39 2.06 19.27 2.28 9.07 SPEIGHT NF 3 2561 4444 173.41 76 71 19.1 39 2.05 18.94 2.45 8.36 SPEIGHT 168 2503 4300 170.61 69 69 18.4 40 2.18 19.30 2.57 8.22 SPEIGHT 179 2566 4444 172.81 73 71 18.6 38 2.05 18.11 2.82 6.74 SPEIGHT 190 2432 4186 171.69 74 72 19.3 40 2.07 19.47 2.72 7.60 GL 737 2077 3574 171.56 76 68 19.2 40 2.12 18.73 2.83 6.98 PO 7 2364 4078 171.68 69 67 18.8 40 2.16 21.18 2.60 9.02 RG7H9 2278 3916 171.51 72 71 19.5 39 2.01 17.62 2.59 7.58 RG7H10 2564 4473 173.92 77 69 18.2 40 2.20 20.04 2.46 8.75 SPEIGHT H2O 2422 4150 171.03 73 68 19.1 42 2.17 20.12 2.41 9.32 PO 1 2404 4150 172.15 77 68 18.5 40 2.15 21.07 2.98 7.94 Mean 2460 4240 171.96 73 69 19.0 39 2.07 19.79 2.55 8.51 C.V.% 17.3 18.7 2.1 10.5 4.0 7.2 6.0 7.8 13.5 12.1 22.2 R2 (%) 63 61 44 76 74 58 75 62 80 83 81 L.S.D 528 1048 7.11 17 4.4 2.6 3 0.28 3.29 0.34 1.99

30 TABLE 7. COMPARISON OF VARIETIES FOR CERTAIN CHARACTERISTICS AT KINSTON NC - 2000. CURED LEAF ANALYSIS LEAVES NODE RED. TOT. RATIO YIELD VALUE INDEX GRADE PER PLANT SPACE SUG. ALK. SUG. VARIETY LBS/A $/A $/CWT. INDEX PLANT HEIGHT INCHES % % ALK. COKER 371-GOLD 2278 3943 173.07 85 19.0 42 2.19 12.93 3.45 3.74 GL 939 2196 3759 171.20 84 20.3 39 1.94 14.93 3.38 4.45 K 149 2210 3852 174.23 82 20.5 43 2.09 11.57 3.38 3.53 K 326 2426 4156 171.59 79 19.6 41 2.09 12.07 2.98 4.07 K 346 2384 4111 172.38 84 19.9 44 2.19 16.87 2.90 5.87 K 358 2265 3921 172.91 83 19.1 43 2.24 12.20 3.40 3.67 K 394 2433 4201 172.43 86 19.5 42 2.13 19.27 2.69 7.19 K 399 2384 4087 171.30 75 19.5 41 2.11 13.47 3.15 4.39 K 730 2460 4282 174.07 82 19.8 44 2.20 14.97 3.12 4.86 NC 55 2465 4299 174.39 86 18.6 38 2.04 14.93 3.15 4.78 NC 71 2785 4844 173.92 84 18.6 40 2.18 13.70 3.13 4.50 NC 72 2571 4486 174.46 84 20.5 45 2.20 14.77 3.06 4.90 NC 297 2670 4674 175.01 81 18.2 41 2.26 16.70 2.76 6.08 NC 606 2274 3908 171.86 81 19.1 44 2.28 14.97 3.31 4.60 OX 207 2064 3530 170.97 76 18.3 40 2.21 12.37 3.43 3.64 OX 414 NF 2095 3597 171.62 81 19.1 43 2.28 14.00 3.16 4.41 RGH4 2269 3898 171.84 81 19.9 43 2.19 10.63 3.91 2.93 RGH12 2625 4545 173.16 81 19.9 43 2.15 15.43 3.01 5.20 RG 17 2257 3901 172.49 76 19.3 41 2.15 13.97 3.07 4.62 RGH51 2277 3940 173.02 80 20.7 43 2.09 15.37 2.71 5.77 RGH61 2148 3647 169.90 71 19.3 44 2.30 14.60 3.00 5.12 RG 81 2322 4022 173.22 80 18.5 40 2.18 14.70 2.85 5.17 SPEIGHT NF 3 2244 3873 172.65 79 19.9 42 2.13 12.10 3.09 3.94 SPEIGHT 168 2368 4029 170.24 78 18.6 41 2.21 14.00 3.23 4.37 SPEIGHT 179 2493 4286 171.95 81 18.6 41 2.22 15.73 3.50 4.53 SPEIGHT 190 2311 3968 171.62 80 20.5 45 2.20 14.47 3.30 4.39 GL 737 2074 3580 172.59 80 20.5 44 2.14 14.47 3.40 4.27 PO 7 2218 3780 170.38 79 19.5 44 2.27 16.03 3.51 4.66 RG7H9 2328 4053 174.08 84 20.3 43 2.09 11.73 3.33 3.54 RG7H10 2625 4583 174.51 85 19.7 43 2.20 15.40 3.07 5.04 SPEIGHT H2O 2282 3924 171.91 82 20.1 46 2.27 13.23 3.13 4.27 PO 1 2265 3894 171.91 80 20.3 44 2.19 15.33 3.93 3.98 Mean 2346 4049 172.53 81 19.5 42 2.18 14.28 3.20 4.58 C.V.% 8.2 8.4 1.3 7.3 5.4 4.3 4.8 24.2 9.9 27.4 R 2 (%) 56 58 38 40 49 70 52 35 60 48 L.S.D 372 645 6.75 20 2.8 3 0.24 12.78 0.60 3.40

31 TABLE 8. COMPARISON OF VARIETIES FOR CERTAIN CHARACTERISTICS AT ROCKY MOUNT NC - 2000. CURED LEAF ANALYSIS DAYS LEAVES NODE RED. TOT. RATIO YIELD VALUE INDEX GRADE TO PER PLANT SPACE SUG. ALK. SUG. VARIETY LBS/A $/A $/CWT. INDEX FLOWER PLANT HEIGHT INCHES % % ALK. COKER 371-GOLD 1734 3003 172.00 64 65 17.0 33 1.93 20.80 3.22 6.49 GL 939 2244 3851 171.25 57 67 19.1 35 1.83 23.80 2.50 9.52 K 149 1616 2743 169.56 55 63 18.0 37 2.09 23.10 2.23 10.62 K 326 2525 4416 173.97 63 67 17.6 36 2.09 24.20 2.07 11.69 K 346 2109 3620 171.52 55 64 19.6 38 1.97 21.97 2.44 9.03 K 358 2142 3706 172.83 65 67 19.9 35 1. 74 23.40 2.14 11.37 K 394 1978 3378 170.59 61 67 17.6 38 2.17 25.40 2.05 12.68 K 399 1974 3346 168.73 53 63 18.5 34 1.85 23.30 2.22 11.20 K 730 2088 3660 174.72 69 65 18.5 33 1.82 22.93 2.24 10.40 NC 55 2514 4423 176.19 70 64 18.9 36 1.90 24.40 2.51 9.76 NC 71 2432 4196 172.18 59 65 17.4 34 1.97 22.83 2.56 9.18 NC 72 2496 4292 171.64 55 68 17.6 37 2.12 23.70 2.24 10.67 NC 297 1837 3056 166.30 46 64 19.1 35 1.87 24.10 2.26 11.57 NC 606 2097 3700 176.29 78 66 18.6 35 1.87 24.87 2.28 11.06 OX 207 1959 3306 168.79 54 67 17.9 32 1.80 22.87 2.66 8.66 OX 414 NF 2133 3781 177.26 79 72 22.1 40 1.84 22.70 2.02 11.85 RGH4 2017 3523 173.55 55 65 19.1 39 2.07 21.83 2.35 10.02 RGH12 1825 3018 165.21 52 67 18.7 35 1.86 21.93 2.51 8.96 RG 17 2459 4345 176.35 75 67 17.4 36 2.07 22.83 2.34 9.89 RGH51 2214 3699 167.05 47 68 18.2 35 1.94 24.27 2.43 10.09 RGH61 1813 3067 168.98 56 65 18.3 36 1.96 22.80 2.37 9.61 RG 81 2209 3775 170.55 55 66 19.1 37 1.93 21.90 2.06 10.64 SPEIGHT NF 3 2590 4510 174.27 67 68 18.1 36 1.96 24.10 2.24 10.85 SPEIGHT 168 2038 3497 169.70 53 67 17.7 40 2.25 21.90 2.28 9.85 SPEIGHT 179 1988 3450 172.72 57 66 18.9 34 1.79 19.57 2.43 8.13 SPEIGHT 190 2195 3856 174.62 65 71 17.9 37 2.09 23.00 2.53 9.13 GL 737 1578 2693 169.75 66 66 18.3 37 2.09 21.63 2.78 7.95 PO 7 1705 2889 169.36 49 64 17.5 37 2.15 23.17 2.14 10.98 RG7H9 1974 3379 170.58 59 66 19.5 37 1.94 20.90 2.16 9.80 RG7H10 1958 3370 171.39 62 66 16.3 38 2.32 22.50 2.08 11.18 SPEIGHT H2O 1952 3303 169.32 56 66 17.1 36 2.09 23.37 2.22 10.55 PO 1 2172 3773 172.07 70 64 17.4 36 2.08 22.27 2.86 7.91 Mean 2080 3582 171.54 60 66 18.3 36 1.98 22.88 2.36 10.04 C.V.% 21.0 22.8 2.6 16.9 3.9 8.3 7.0 10.9 7.8 13.9 19.4 R 2 (%) 39 38 41 51 47 54 49 47 43 56 48 L.S.D 1358 2562 12.55 21 6 3.8 6 0.61 4.63 0.72 5.21

32 TABLE 9. COMPARISON OF VARIETIES FOR CERTAIN CHARACTERISTICS AT OXFORD NC - 2000. CURED LEAF ANALYSIS DAYS LEAVES NODE RED. TOT. RATIO YIELD VALUE INDEX GRADE TO PER PLANT SPACE SUG. ALK. SUG. VARIETY LBS/A $/A S/CWT. INDEX FLOWER PLANT HEIGHT INCHES % % ALK. COKER 371-GOLD 2439 4120 168.55 77 74 17.0 38 2.21 23.97 2.19 10.94 GL 939 2778 4757 171.17 79 72 19.3 38 2.00 19.93 2.47 8.56 K 149 2982 5116 171.52 79 74 18.9 39 2.07 23.50 1.87 12.59 K 326 2967 5059 170.47 76 74 19.2 37 1.93 22.00 1.89 11.99 K 346 3110 5385 173.14 78 70 20.3 40 1.97 23.07 2.17 10.61 K 358 2805 4876 173.43 84 74 18.3 40 2.19 22.03 2.08 10.69 K 394 2904 4757 163.68 56 75 19.3 37 1.95 25.13 1.50 16.93 K 399 3033 5172 170.13 72 72 20.1 37 1.87 20.63 1.91 11.16 K 730 2661 4461 167.65 72 72 18.9 38 2.04 23.23 1.97 11.86 NC 55 3249 5653 173.98 80 73 19.9 40 2.00 21.03 2.12 10.05 NC 71 3248 5587 171.83 74 75 19.0 37 1.94 22.90 2.01 11.49 NC 72 3050 5341 174.95 84 73 18.2 40 2.21 20.67 2.03 10.34 NC 297 3605 6273 173.50 77 72 19.1 39 2.08 21.77 2.10 10.43 NC 606 2759 4824 174.42 85 72 17.0 40 2.34 25.03 2.10 11.94 OX 207 2520 4267 168.01 73 76 19.5 38 1.94 21.63 2.27 9.85 OX 414 NF 3356 5954 177.24 85 73 20.1 41 2.04 21.17 2.05 10.31 RGH4 3313 5730 172.64 78 70 18.9 43 2.29 23.83 2.41 9.95 RGH12 2341 3964 168.94 78 73 19.7 37 1.89 22.87 1.85 12.37 RG 17 2807 4728 168.20 70 72 19.5 38 1.99 22.53 1.97 11.79 RGH51 2856 4997 173.49 83 74 18.4 40 2.17 21.37 2.26 9.94 RGH61 3241 5652 173.37 82 72 18.9 40 2.13 22.17 1.97 11.68 RG 81 3302 5703 172.42 72 71 19.1 39 2.06 21.20 1.93 11.41 SPEIGHT NF 3 2850 4948 173.31 82 73 19.3 39 2.07 20.63 2.02 10.28 SPEIGHT 168 3104 5373 171.89 76 72 18.7 38 2.08 22.00 2.20 10.44 SPEIGHT 179 3218 5595 173.77 79 75 18.4 39 2.13 19.03 2.54 7.56 SPEIGHT 190 2790 4732 168.82 77 73 19.5 38 1.94 20.93 2.33 9.28 GL 737 2578 4451 172.35 82 70 18.7 39 2.12 20.10 2.32 8.71 PO 7 3169 5565 175.30 80 70 19.5 40 2.05 24.33 2.14 11.42 RG7H9 2532 4316 169.85 74 75 18.5 37 2.01 20.23 2.29 9.41 RG7H10 3109 5467 175.85 83 73 18.6 38 2.08 22.23 2.23 10.03 SPEIGHT H2O 3033 5221 171.87 81 70 20.1 43 2.16 23.77 1.87 13.13 PO 1 2774 4785 172.47 81 72 17.8 38 2.17 25.60 2.16 11.93 Mean 2953 5088 171.82 78 73 19.0 39 2.07 22.20 2.10 10.91 C.V.% 18.9 20.6 2.2 7.9 4.1 7.8 6.8 7.0 11.2 13.4 21.2 R 2 (%) 30 30 47 60 34 55 37 70 39 48 45 L.S.D 2020 3764 9.40 12 10 5.3 9 0.32 7.08 0.67 5.80

33 TABLE 10. COMPARISON OF ADVANCED BREEDING LINES FOR CERTAIN CHARACTERISTICS FOR FOUR LOCATIONS - 2000. CURED LEAF ANALYSIS DAYS LEAVES NODE RED. TOT. RATIO YIELD VALUE INDEX GRADE TO PER PLANT SPACE SUG. ALK. SUG. VARIETY LBS/A $/A $/CWT. INDEX FLOWER PLANT HEIGHT INCHES % % ALK. NC 2326 2661 4563 171.48 67 67 18.0 41 2.28 19.17 2.97 6.66 NC 95 2811 4869 173.33 71 72 19.6 41 2.10 20.69 2.81 7.91 K 326 3226 5713 177.10 81 73 20.3 39 1.94 20.44 2.23 10.20 AVH 201 3249 5713 175.78 74 73 20.8 41 2.00 20.81 2.50 9.03 NC TG 91 3226 5704 176.77 77 73 20.4 39 1.91 20.35 2.66 8.01 NC TG 115 3086 5426 175.86 79 73 20.5 39 1.89 19.53 2.79 7.67 NC TG 116 3259 5799 177.87 82 74 20.2 39 1.95 20.32 2.48 8.70 NC TG 117 3115 5501 176.43 79 72 20.6 38 1.86 20.53 2.77 7.98 NC TG 118 2973 5230 175.82 76 70 19.8 39 1.98 20.01 2.74 8.16 OX 9062 3285 5791 176.41 74 74 21.3 39 1.84 19.39 2.59 8.29 OX 9150 3211 5626 175.32 73 73 21.0 37 1.79 20.90 2.78 8.13 OX 9175 3046 5352 176.19 74 75 21.0 40 1.90 20.13 2.45 8.96 OX 9210 2906 5013 172.36 70 72 20.6 35 1.73 20.28 2.34 9.53 OX 9230 3087 5459 176.79 77 74 21.1 38 1.81 18.38 2.93 6.62 OX 9377 3107 5369 172.36 68 70 19.0 40 2.13 21.64 2.51 8.90 RG8H41 2984 5273 176.66 78 73 20.6 41 2.01 19.70 2.36 8.74 RG426NF 3177 5556 174.68 72 77 22.4 38 1.71 18.41 2.60 7.30 RG8H40 3413 6078 177.98 76 71 19.2 41 2.12 20.69 2.66 8.29 RG8H28 3099 5452 175.72 77 70 20.8 40 1.93 21.12 2.51 8.99 RG8H33 3344 5932 177.39 78 72 20.3 39 1.93 19.37 2.61 7.90 SPEIGHT H6 3210 5632 175.47 74 72 19.8 40 2.01 20.88 2.36 9.35 SPEIGHT H 17 3260 5702 174.97 69 72 20.3 41 2.01 20.66 2.42 9.17 SPEIGHT 220 2890 5091 176.14 75 75 20.3 39 1.95 19.77 2.63 7.93 SPEIGHT 221 2926 5091 174.07 70 72 19.8 41 2.08 20.03 2.58 8.34 SPEIGHT 222 2993 5269 176.06 77 72 21.0 38 1.84 19.38 2.50 8.81 SPEIGHT 223 3169 5450 171.59 62 75 20.2 41 2.02 19.73 3.11 6.78 ULT 28 3142 5509 175.50 72 72 20.8 39 1.91 21.24 2.66 8.62 ULT 103 3021 5279 174.78 71 71 19.8 40 2.02 19.43 2.89 7.36 Mean 3102 5444 175.39 74 72 20.3 39 1.95 20.10 2.62 8.30 C.V.% 10.5 11.0 1.7 11.2 3.0 6.1 5.8 7.1 9.8 11.2 17.2 R2 (%) 69 68 59 55 83 68 66 78 85 83 87 L.S.D 270 486 3.0 8 3 1.5 2 0.13 2.90 0.27 1.76

34 TABLE 11. COMPARISON OF ADVANCED BREEDING LINES FOR CERTAIN CHARACTERISTICS AT WHITEVILLE NC - 2000. CURED LEAF ANALYSIS DAYS LEAVES NODE RED. TOT. RATIO YIELD VALUE INDEX GRADE TO PER PLANT SPACE SUG. ALK. SUG. VARIETY LBS/A $/A $/CWT. INDEX FLOWER PLANT HEIGHT INCHES % % ALK. NC 2326 2505 4248 169.61 60 66 17.2 44 2.56 20.77 3.46 6.03 NC 95 2545 4430 173.81 63 70 18.3 40 2.16 19.70 3.37 5.95 K 326 3214 5676 176.59 79 69 19.9 40 2.03 19.13 2.73 7.37 AVH 201 3091 5460 176.60 74 71 20.6 41 2.00 19.37 3.10 6.26 NC TG 91 3222 5702 177.02 76 70 19.8 39 1.97 21.83 3.15 6.99 NC TG 115 2760 4832 175.03 77 70 18.4 35 1.90 19.67 3.37 5.97 NC TG 116 3002 5355 178.10 79 72 19.3 40 2.05 20.37 2.85 7.14 NC TG 117 2698 4725 174.90 74 70 20.3 36 1.77 21.10 3.01 7.15 NC TG 118 2837 5026 176.88 77 69 18.0 40 2.21 16.77 3.66 4.62 OX 9062 2975 5232 176.01 73 72 21.5 40 1.86 19.87 2.97 6.72 OX 9150 2947 5233 177.61 76 72 19.8 37 1.90 20.00 3.27 6.15 OX 9175 2436 4269 175.65 69 71 20.2 39 1.96 16.50 3.02 5.78 OX 9210 2550 4351 170.68 66 70 19.9 34 1.72 19.30 2.90 6.69 OX 9230 2803 4964 177.00 77 71 21.3 40 1.89 17.33 3.58 5.02 OX 9377 2843 4880 170.58 67 70 18.0 39 2.17 21.43 2.82 7.81 RG8H41 2874 5060 176.08 77 71 19.2 40 2.11 19.57 2.62 7.63 RG426NF 3028 5235 172.70 62 77 22.7 37 1.64 20.27 3.00 6.85 RG8H40 3236 5774 178.45 77 69 18.1 42 2.34 19.93 3.16 6.68 RG8H28 2898 5115 176.53 77 70 19.2 40 2.11 20.40 3.05 6.71 RG8H33 2943 5197 176.50 76 71 19.9 40 1.99 16.57 3.35 5.12 SPEIGHT H6 3041 5385 177.03 81 70 18.8 40 2.14 19.67 2.86 6.88 SPEIGHT H17 3041 5283 173.85 67 70 19.2 41 2.12 20.90 2.67 7.98 SPEIGHT 220 2479 4312 173.69 72 73 18.0 38 2.14 19.43 3.15 6.35 SPEIGHT 221 2831 5003 176.73 78 69 19.4 42 2.16 18.50 3.13 6.09 SPEIGHT 222 2667 4691 175.84 74 70 19.5 37 1.88 18.13 2.92 6.27 SPEIGHT 223 2981 5011 166.91 50 73 19.2 39 2.03 20.07 3.60 5.65 ULT 28 3097 5419 175.05 68 69 20.1 41 2.02 19.57 3.43 5.93 ULT 103 2627 4584 174.53 67 70 18.6 40 2.17 17.73 3.68 4.98 Mean 2863 5016 175.00 72 70 19.4 39 2.04 19.42 3.14 6.38 C.V.% 12.1 12.7 1.9 10 4 2.5 5 4.7 6 12.9 9.7 19.7 R2 (%) 49 51 57 58 64 70 67 79 60 68 63 L.S.D 948 1667 6.77 14 3 1.6 3 0.19 8.26 0.55 3.33

35 TABLE 12. COMPARISON OF ADVANCED BREEDING LINES FOR CERTAIN CHARACTERISTICS AT KINSTON NC - 2000. CURED LEAF ANALYSIS LEAVES NODE RED. TOT. RATIO YIELD VALUE INDEX GRADE PER PLANT SPACE SUG. ALK. SUG. VARIETY LBS/A $/A $/CWT. INDEX PLANT HEIGHT INCHES % % ALK. NC 2326 3271 5760 176.05 82 18.4 43 2.37 13.83 2.92 4.77 NC 95 3190 5649 176.93 83 19.8 44 2.20 16.63 3.39 4.93 K 326 3663 6523 178.06 85 19.4 42 2.15 15.80 2.69 5.87 AVH 201 3437 6046 175.92 78 20.2 43 2.14 15.97 2.88 5.72 NC TG 91 3499 6203 177.24 80 19.4 40 2.08 14.73 3.03 4.89 NC TG 115 3589 6350 176.93 82 20.3 42 2.07 13.43 3.18 4.48 NC TG 116 3617 6405 177.08 85 19.0 41 2.19 14.63 2.88 5.09 NC TG 117 3533 6286 177.98 81 19.1 40 2.10 13.00 3.47 3.81 NC TG 118 3279 5779 176.21 76 19.3 40 2.10 15.83 3.02 5.29 OX 9062 3267 5740 175.61 75 19.1 41 2.16 12.10 3.25 3.73 OX 9150 3531 6272 177.63 81 19.2 38 1.97 16.00 3.38 4.81 OX 9175 3498 6223 177.91 87 19.9 42 2.10 16.03 2.79 5.80 OX 9210 3090 5421 175.48 77 17.9 35 1.98 14.73 2.74 5.51 OX 9230 3430 6106 178.02 83 19.5 39 1.99 15.33 3.07 5.23 OX 9377 3469 6080 175.22 78 19.1 43 2.26 18.27 2.72 6.76 RG8H41 3027 5312 174.72 78 20.6 43 2.10 15.60 2.56 6.16 RG426NF 3600 6381 177.15 76 22.3 42 1.87 13.47 2.68 5.03 RG8H40 3645 '6487 177.90 85 19.3 42 2.19 16.17 2.93 5.58 RG8H28 3410 6036 176.84 81 20.2 43 2.13 15.60 2.85 5.55 RG8H33 3609 6413 177.70 79 19.9 42 2.12 15.97 2.66 6.03 SPEIGHT H6 3395 6020 177.30 82 20.3 42 2.07 16.13 2.61 6.22 SPEIGHT H17 3417 5977 174.98 73 19.8 43 2.17 13.60 2.92 4.66 SPEIGHT 220 3058 5428 177.47 81 19.9 42 2.11 15.93 2.90 5.49 SPEIGHT 221 3541 6139 173.43 67 19.3 44 2.28 15.50 2.96 5.26 SPEIGHT 222 3271 5734 175.27 81 19.7 42 2.14 14.27 3.26 4.44 SPEIGHT 223 3474 6130 176.44 76 20.3 43 2.11 13.33 3.67 3.72 ULT 28 3101 5479 176.73 80 19.7 42 2.13 16.90 2.91 5.91 ULT 103 3216 5620 174.75 80 19.2 43 2.23 13.70 3.14 4.43 Mean 3397 5999 176.53 80 19.6 42 2.12 15.09 2.98 5.19 C.V.% 9.3 9.7 0.8 7.0 5.4 3.8 6.2 13.4 11.0 20.5 R 2 (%) 37 38 58 48 50 68 53 45 52 46 L.S.D 983 1765 2.84 13 2.5 3 0.31 5.28 0.68 2.63