Leaf removal: a tool to improve crop control and fruit quality in vinifera grapes

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Michigan Grape & Wine Industry Council 2015 Report of Research Activities Leaf removal: a tool to improve crop control and fruit quality in vinifera grapes PI Paolo Sabbatini Dept. of Horticulture, Michigan State University, East Lansing, MI 48824 Collaborators Glen Greiffendorf 1, Pat Murad 2, Cristian Paulsen 2 and Tommaso Frioni 3 1 12 Corners Vineyards & Winery 1201 N Benton Center Rd, Benton Harbor, MI 49022 2 Dept. of Horticulture, Michigan State University, East Lansing, MI 48824 3 Dept. of Horticulture, Michigan State University, East Lansing, MI 48824

Research Report 2015 Leaf removal: a tool to improve crop control and fruit quality in vinifera grapes 2015: Impact of Early leaf removal on Riesling 2012: Timing and intensity of early leaf removal on Chardonnay and Pinot noir 2013: Timing and intensity of early leaf removal on Chardonnay and Pinot noir and potential carry over effects 2014: Physiology of early leaf removal, impact on carbon partitioning and effect of pruning and training strategies on Pinot noir 2016: Mechanization of early leaf removal, impact on bunch cluster rot (sour rot) on Merlot and Chardonnay 2017: Mechanization of early leaf removal, impact on bunch cluster rot (sour rot) on Merlot and Chardonnay 2017-18: Physiological Impact of cluster zone early leaf removal on vine carbon allocation and fruit secondary metabolites (metabolomics) on Pinot noir

Project: Leaf removal: a tool to improve crop control and fruit quality in vinifera grapes Report 2015: Impact of Early leaf removal on Riesling This work was partially financially supported by AgBioResearch at Michigan State University (Project GREEEN), the Michigan Grape and Wine Industry Council and the MSU Southwest Michigan Research and Extension Center. This information is given in summary form of a research in progress and does not purport to be complete and should not be considered as final advice or a final recommendation to grape growers unless extensively tested by MSU field extension specialists, farm advisors and industry members.

Project: Leaf removal: a tool to improve crop control and fruit quality in vinifera grapes Report 2015: Impact of Early leaf removal on Riesling Research Component: research trial at MSU Southwest Michigan Research and Extension Center (Benton Harbor) Extension Component: demonstration trial at 12 Corners Vineyards & Winery (1201 N Benton Center Rd, Benton Harbor, MI 49022) on Cabernet franc and Pinot noir

Aim of Leaf Removal (classical approach) Cool climate Disease control (Palliotti et al., 2012; Hed et al., 2015) Veraison Better sun exposure (Gatti et al., 2015; Mosetti et al., 2016) Lemon Creek Winery, 2016

Aim of Leaf Removal Leaf Removal NO Leaf Removal The varieties that are cold resistant and early ripening for cool climate viticulture are highly sensitive to fruit rot Improving Fruit Technological Maturity Data from SWMREC (2000-2015)

Research on Leaf Removal (literature review) Bud Break Pre-Bloom Bloom After-Bloom Fruit-Set Veraison Harvest Yield Reduction Quality Increase Disease Reduction PB B AB FS V 0 Publication Frequency 5

Research on Mechanical Leaf Removal Plucker, Tanesini Technology Gregoire DX20 Leaf Remover DF200 BMV Double model leaf remover Collard France Compressed air blown to the canopy

Research on Leaf Removal (at MSU) PB B FS 50 % Yield Reduction 29 % 33 % 5 % Quality Increase (⁰Brix) 11 % 3 % 2 % Disease Reduction 71 % 70 % 35 % Pinot noir Chardonnay Merlot Cabernet Franc 0 4 6 8 10

The Physiological Reason Sabbatini P., Sivilotti P., Acimovic D., Sternard Lemud M. and U. Vrhovsek. 2016. Physiological impact of cluster zone leaf removal on vine carbon allocation and fruit secondary metabolites. X ISHS International Symposium on Grapevine Physiology and Biotechnology With early leaf removal a significant amount of photosynthetically active leaves is removed. The vine undergoes a temporary situation a stress that results in a lower berry set, thus the clusters have a reduced number of berries and so also a reduced compactness. With later applications of leaf removal no modifications of number of berries would be obtained.

Data to Support Sabbatini et al, 2012

Leaf removal: a tool to improve crop control and fruit quality in vinifera grapes The Case of Riesling Location was South West Research and Extension Center, Benton Harbour, Michigan Cultivar used in the experiments was Riesling clone 49 trained in a Scott Henry trellising system The six basal leaves at full bloom removed from the upper cordon. Lower cordon was not included in the experiments.

Control vs. Leaf Removal

Impact on Fruit Set Number of flowers Number of berries % Fruit set Treatment Mean SD ± Mean SD ± Mean SD ± Control 187.2 a 61.50 115.7 a 48.17 63 a 19.08 Defoliated 208.2 a 61.32 88.9 b 34.42 43 b 15.23 Defoliation treatment reduced fruit set by 30% in relation to nondefoliated control

Bunch Cluster Rot Number of infected berries Percentage of infected berries Treatment Mean SD ± Mean SD ± Control 30.7 a 15.1 35.9 a 12.9 Defoliated 8.5 b 4.9 11.8 b 10.8 Significance * * Significant difference between the two groups in absolute terms and in relative terms. This corresponds to what has been found in other research and other varieties

Berry Analysis Pulp Weight Berry Weight Brix (g) (g) Treatment Mean SD ± Mean SD ± Mean Control 1.41 a 0.29 1.9 a 0.28 17.6 a Defoliated 1.21 a 0.26 1.8 a 0.37 18.7 a S D ± 1. 69 1. 42 Total Total Acidity Terpenes (g/l) (mg/l) Mean SD ± Mean SD ± 7.5 a 1.41 1.46 a 0.80 7.1 a 1.41 1.61 a 1.34 Significance ns ns ns ns ns

Summary DIRECT Reduction in fruit set Reduction of yield per vine (20-30%) Increase the number of buds per vine to accommodate the yield reduction? INDIRECT Reduction in bunch rot Not increase quality contrarily to Pinot noir, Chardonnay, Merlot and Pinot Gris Further research on-going APPLICATION Pre-bloom and bloom applications Fruit set not effective to reduce berries per cluster and cluster morphology