XII International Conference on Grapevine Breeding and Genetics (Bordeaux, July 15-20, 2018) Recovering ancient grapevines varieties in the Spanish provinces of Alicante and Valencia A. Yuste, F. Martínez-Gil, S. García-Martínez, D. López Lluch; R. Peiró R, C. Jiménez, J.J. Ruiz and C. Gisbert Other researchers collaborating to reach this goal are from MINECO PROJECT CGL2015-70843-R FEDER FUNDS
XII International Conference on Grapevine Breeding and Genetics, Bordeaux, 2018 Comunitat Valenciana Alt de Benimaquia Denia PROTECTED DESIGNATION OF ORIGIN (PDO) Wine elaboration VII bc century BC Protected Geographical Indication Castellón
PROJECT CGL2015-70843-R XII International Conference on Grapevine Breeding and Genetics, Bordeaux, 2018 SSR profiles Identitity Synonymies Homonymies Variability Germplams bank In vitro storage Virus cleaning x
XII International Conference on Grapevine Breeding and Genetics, Bordeaux, 2018 Localization of varieties in risk of disapearance New SSR profiles i.e. Planta Mula, Valencí negre, Botó de Gall, Arcos, Forcallat, Verdilet, Mamella de vaca, Raïm del clotet, Esclafagerres, etc. Some of the synonymies found Grumer Moscatell Moscatel del terreno Moscatel d Alfàbega Gustico de Elche Moscatel Dulce Moscatel de Alicante Raïm del Clotet Muscat d Istamboul (variety 17493 in VIVC) (Valencí blanc x Muscat of Alexandria; Lacombe et al. 2013 ; Mena et al. 2014) Esclafagerres Botó de Gall accessions Ahmeur bou Ahmeur (variety 140 in VIVC) Gisbert et al. 2018; Peiró et al. 2018; Jiménez et al. 2019
Analysis of variability XII International Conference on Grapevine Breeding and Genetics, Bordeaux, 2018 Moscatel de Alejandría (Muscat of Alexandria) Monastrell (Mourvedre) AFLPs/Peiró et al. 2018. Spanish Journal of Agricultural Research vol.56(2) GBS/46 accessions, 93.466 SNPs, Neighbour-Joining (NJ) bootstrap 1000. Reference clon MOU-369
0.05 Morenillo Carignan NI-5 Rojal Tinta Planta Nova-1 Planta Nova-2 Alicante Henry Bouchet Ullet de Perdiu Alcañon Verdil-1 Verdil-2 Mari Sancho Trepadell Planta Fina REF Planta Fina Alarije Merseguera Bobal REF Bobal Planta Mula Morsí (Sbaa Tolba) Macabeo Xarello REF Esclafagerres Tortosina Valencí Negre Valencí Blanc-3 Valencí Blanc-1 Valencí Blanc-2 NI-1 REF Bonicaire Forcallat Tinta Arcos Montalbana REF Benicarló Monastrell REF Monastrell Raïm del Clotet NI-3 NI-4 Morastell REF Pampolat Crujidera (Marufo) REF Gateta Mondragón NI-2 Botó de Gall (Ahmeur bou Ahmeur) Mamella de Vaca Afus Ali-1 (Rosetti) Afus Ali-2 (Rosetti) Alfonse Lavalleé Cardinal Cornichon Blanc Dominga Grumer Moscatell (Muscat d Istanbul) Muscat of Alexandria REF Moscatel E-110 Moscatel de Hamburgo Italia SSRs/Jiménez et al. 2018/9 53% chlorotype A 23% chlorotype D 13% chlorotype B 11 % chlorotype C Interand Intravarietal variability
Analysis of variability XII International Conference on Grapevine Breeding and Genetics, Bordeaux, 2018 Moscatel de Alejandría (Muscat of Alexandria) Monastrell (Mourvedre) Peiró et al. 2018. Spanish Journal of Agricultural Research vol.56(2) 46 accesions, 93.466 SNPs, Neighbour-Joining (NJ) bootstrap 1000. Reference clon MOU-369
0.05 Morenillo Carignan NI-5 Rojal Tinta Planta Nova-1 Planta Nova-2 Alicante Henry Bouchet Ullet de Perdiu Alcañon Verdil-1 Verdil-2 Mari Sancho Trepadell Planta Fina REF Planta Fina Alarije Merseguera Bobal REF Bobal Planta Mula Morsí (Sbaa Tolba) Macabeo Xarello REF Esclafagerres Tortosina Valencí Negre Valencí Blanc-3 Valencí Blanc-1 Valencí Blanc-2 NI-1 REF Bonicaire Forcallat Tinta Arcos Montalbana REF Benicarló Monastrell REF Monastrell Raïm del Clotet NI-3 NI-4 Morastell REF Pampolat Crujidera (Marufo) REF Gateta Mondragón NI-2 Botó de Gall (Ahmeur bou Ahmeur) Mamella de Vaca Afus Ali-1 (Rosetti) Afus Ali-2 (Rosetti) Alfonse Lavalleé Cardinal Cornichon Blanc Dominga Grumer Moscatell (Muscat d Istanbul) Muscat of Alexandria REF Moscatel E-110 Moscatel de Hamburgo Italia Jiménez et al. 2018/9 53% chlorotype A 23% chlorotype D 13% chlorotype B 11 % chlorotype C Interand Intravarietal variability
UE2002/11/CE Virus analysis XII International Conference on Grapevine Breeding and Genetics, Bordeaux, 2018 Grapevine fanleaf virus (GFLV) Grapevine leafroll associated virus 1 (GLRaV-1) Grapevine leafroll associated virus 3 (GLRaV-3) Grapevine fleck virus (GFkV) Arabis mosaic virus (ArMV) x High incidence 35% simple 37% doble 23% triple Development of protocols for virus cleaning Meristem culture Somatic embryogenesis
Development of protocols for in vitro storage XII International Conference on Grapevine Breeding and Genetics, Bordeaux, 2018
XII International Conference on Grapevine Breeding and Genetics, Bordeaux, 2018 Thank you/merci