GUIDE VEGAN wines TECHNOLOGICAL

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www.martinvialatte.com VEGAN wines GUIDE TECHNOLOGICAL

The Origins of Veganism Veganism is a movement that began in the United Kingdom around 7 years ago, initiated by Donald Watson and inspired by several great thinkers like Voltaire and De Vinci. It consists of refusing to exploit animals in any way. Stricter than vegetarians, who do not eat meat, in addition to not consuming food that comes from animals, vegans do not consume any products that REPRESENTATION OF VEGETARIANS ACROSS THE WORLD have caused suffering to an animal. Reliable data on vegan populations is rare. However, statistics estimate that the population of vegans in the USA (the largest in the world) has doubled since 9 and is close to.5%. Less than 3% 1% and above Between 5 and 9.9% Between 3 and 4.9% And wine does not escape that rule. Above and beyond the allergenic potential of some animal proteins like casein, egg albumin and isinglass, some wine drinkers lead a vegan lifestyle. They aim to eliminate the use of any animals in wine-growing and wine-making. Many vegan logos can now be found on bottles of wine (first logo on wine in France in 14). Veganism is a doctrine according to which humans should live without exploiting animals.

Developing alternatives to animal-based fining agents 1 Plant proteins as alternatives to gelatins Aware of this evolution in consumer trends, the company Martin Vialatte has been working on the development of non-animal fining agents for over 15 years. Indeed, in 1997, the outbreak of Creutzfeld-Jacob s disease was already calling the use of these products into question. The development of these new fining agents is clearly in keeping with the vegan philosophy of some consumers. The first efforts to replace gelatin as a fining agent by plant-based proteins were made in 1997. At that time, around 15 proteins from various plant origins were sampled (rice, wheat, corn, soy, lupin, potato, pea etc.) then tested in laboratories to determine their affinities for polyphenols and their influence on the organoleptic characteristics of wines. Some were discarded due to risks relating to GMOs or allergens, while others were judged to be ineffective. At the end of the laboratory testing process and the 3 years of industrial testing in wine cellars, pea proteins were selected. Martin Vialatte also referred the project to the OIV, so that in 5 the fining of musts and wines with pea proteins was adopted as a new oenological practice. The ProVgreen range was created after this authorization, and today brings together 4 specialties composed uniquely of pea proteins: ProVgreen Pure Must for the clarification of musts, ProVgreen Pure Wine for the fining of wines, ProVgreen Flot for the clarification of musts by flotation and ProVgreen L17, a liquid version of pea proteins for the clarification and fining of musts and wines. This range has been recognized and honored on several occasions (VINITECH 6, INTERVITIS 1). Replacing products of animal origin and allergens In 8, the R&D department at Martin Vialatte initiated the replacement of products made using casein, egg albumin and isinglass with formulations based on plant proteins. In addition to being unsuitable for vegan wines, these animal products are also potentially allergenic for consumers. Numerous tests carried out in laboratories and wine cellars have allowed alternatives to these products of animal origin and allergens to be developed. 3 Chitin derivatives: new tools for winemaking For several years, chitin derivatives have been available for treating musts and wines. Chitin is a polysaccharide that is widespread in nature and mainly used in the agro-food, cosmetics and health industries. Chitin is mainly produced from crustacean shells, but only chitin of fungal origin is authorized for use in wine-making. Its principal derivatives are chitosan and chitin-glucan. These compounds have several properties: Clarification of musts and wines Improvement to the organoleptic characteristics of wines Reduction of micro-organism populations, in particular Brettanomyces Reduction in Ochratoxin A concentration. Today, Martin Vialatte has two chitin-derived formulations that are non-allergenic and not of animal origin: KTS Control, a specific chitosan-based formulation for the elimination of unwanted micro-organisms, in particular Brettanomyces, and KTS Clear, a specific chitin-glucan-based formulation for eliminating unpleasant tastes (reduction, ethyl phenols) and for clarifying and fining wines.

MARTIN VIALATTE SOLUTIONS There is no official charter specifying the permitted practices or oenological products for making vegan wines. But some key principles are recognized by most vegans. First, the producer must not use animals to cultivate the vines. Then, the oenological products used must not be of animal origin. Finally, whenever possible, it is preferable that vegan wines are made from organic grapes. The Martin Vialatte range today offers several products that are compatible with a vegan lifestyle. These formulations have a mode of action and an impact on the musts and wines similar to fining agents of animal origin, and are important tools for makers of vegan wines. ProVGreen Pure Must Pea proteins selected for the clarification and treatment of musts against oxidation Rapid flocculation of suspended particles in must Removes oxidized or oxidizable phenolic compounds from must Ensures well compacted must deposits Contributes to aromatic freshness and to the preservation of a young tint in white and rosé wines Comparison of deposit compacting with PROVGREEN PURE MUST on Chardonnay musts Turbidity (NTU) 4 3 1 T T 7h T 4h T 48h Time (hours) Control PROVGREEN PURE MUST at 3 g/hl Conclusions At equivalent doses, PROVGREEN PURE MUST display identical results. For fining with products based on plant-proteins, winemakers can choose between products in powder and liquid form. PolyGreen Casein-free compound based on selected pea proteins for the preventive and curative treatment of musts and wines against oxidation Removal of oxidized and oxidizable polyphenols Revitalization of color and aromatic expression Reduction of astringency and removal of bitterness Stabilization of SO through the removal of binding factors Organoleptic profiles of control wine and treated wines Effect of different Chardonnay must treatments on DO8 and DO3 of corresponding wines Bitterness Color 6 5 4 3 1 Fruity Control Casein reference POLYGREEN 14 1 1 8 6 DO 8 DO 3 Dryness Floral 4 Herbaceous Acid balance Control Casein reference POLYGREEN

Products for making vegan wines ProVGreen Pure Wine Selected pea proteins for fining and maturing wines Rapid flocculation with suspended particles in the wine Improved organoleptic characteristics of the wine: elimination of aggressive tannins and bitterness with greater suppleness and roundness on the palate Better aromatic expression of the wine, enhancing the fruity notes Ensures well compacted must deposits, more so than an animal-based fining treatment Good preparation of wines for pre-bottling filtration Better organoleptic result than gelatin and potato proteins Improvement of organoleptic characteristics Tasting results for a Cabernet fined with PROVGREEN PURE WINE Tasters preferences Palate quality Bitterness Nose intensity 1 1 8 6 4 Nose Quantity of tannins Control Gelatin PV 1 PV 18 16 14 1 1 8 Control Gelatin PV 1 PV 6 4 Astringency Herbaceous Roundness PV 1 = PROVGREEN PURE WINE Control Gelatin PROVGREEN PV PURE WINE KTS Control (controls indigenous microorganisms) Specific formulation based on chitosan Removal of undesirable microorganisms Significant decrease in Brettanomyces yeasts Fast acting MICROORGANISMS Brettanomyces Zygosaccharomyces Lactobacillus Pediococcus Oenococcus Saccharomyces c. DOSES AND IMPACT 3-15 g/hl - Elimination >,5 g/hl Population decrease 5 à 18 g/hl - Elimination > 1 g/hl Population decrease 5-1 g/hl Malolactic fermentation delayed Little effect KTS Clear Specific formulation based on chitin-glucan Removal of undesirable molecules, including unpleasant tastes, which may form in wines due to the development of alteration-causing microorganisms Clarification and fining of wines as an alternative to fining agents of animal origin Rapid result in 48 hours TYPE OF WINE CONTROL WINE TREATED AT DOSE OF 1 g/hl OF KTS CLEAR Burgundy Pinot noir Significant reduction Elimination of reduction and perception of fruity notes Alsace Pinot noir Languedoc Chardonnay Alsace Muscat Strong reduction (H S, cabbage) on the nose and palate Mousiness Lacks cleanness on the nose and loss of aromatic intensity Removal of reduction and dryness on a gummy palate Elimination of defect and improved freshness and fruitiness Very clear aromatic improvement and distinctive character of the grape recovered

A protocol Aromatic white STAGE PRODUCT DOSE (g/hl) COMPOSITION PROPERTIES Sulfiting BAKTOL P 1 g/hl Effervescent potassium metabisulfite Grape temperature < C to limit maceration and oxidation HARVEST HARVEST RECEPTION DIRECT PRESSING T < C to preserve flavors. Otherwise, use a harvest heat exchanger. Controlling microorganisms KTS CONTROL 5 g/hl Chitosan Allows indigenous microorganisms to be controlled, avoids excessive sulfiting Passage au froid 8-1 C Enzyming VIAZYM CLARIF + 1 to 15 NTU 1- ml/hl Pectinases Ester production Specific enzymes for the rapid clarification of pectin-rich musts COLD STATIC SETTLING Fining (poured) Fining (pressed) PROVGREEN PURE MUST or PROVGREEN L17 POLYGREEN 1-5 cl/hl 4-8 g/hl Pea proteins Compound based on pea proteins Affects oxidized polyphenols, good compacting of must deposits Helps protein stability Affects color, bitterness and astringency Racking NEO CRISPY g/hl Yeast derivatives Yeasting SO.DELIGHT g/hl Reducing antioxidant compounds (GSH, peptides): avoids changes to tint and protects flavors, freshness. To be added quickly, as soon as the settling process ends Yeast with transferase activity (ester production) gives very aromatic wines T AF =14 C / 16 C mid AF / C where d<11 Nutrition NUTRICELL FULLAROM g/hl Yeast derivatives, amino acids Control of AF, amplified ester production and improved volume on the palate. To be added during yeast inoculation AF Tannin addition density -15 points SUBLIWHITE 5-1 g/hl Condensed tannins, gallotannins Antioxidant, reinforces floral character and citrus notes Oxygenation density O 8 mg/l O Sterol biosynthesis, increased yeast viability. Option of doubling dose if PA is high Nutrition at mid-af End AF / d<1 NUTRICELL FINISH g/hl Full vats Compound based on yeast derivatives Difficult AF: detoxification of conditions, prevents stopping and reactivating AF Avoids oxidation of wines FINING END AF Racking D+3 SO Active SO =,6 mg/l 1 x / per week Potassium metabisulfite PROVGREEN PURE WINE 5-1 g/hl Pea proteins Effective clarification of wine, good compacting of lees KTS CLEAR 1- g/hl Compound based on chitin-glucan Clarifies and improves organoleptic cleanness

A protocol Red, traditional maceration STAGE PRODUCT DOSE (g/hl) COMPOSITION PROPERTIES Vat inertion CO Tapis gaz 4 cm 3 à 5 g/hl CO (g) Protection against oxidation Sulfiting BAKTOL P K Metabisulfite Controls indigenous microorganisms VAT FILLING Enzyming VIAZYM EXTRACT ml/hl Homogenization Remontage Volume cuve Addition of tannins if vatting < 8 days Pectinases and cellulases Deterioration of cell walls to extract polyphenols, polysaccharides and aromas SIMILIOAK,-,4 g/l Stabilization of color Improved volume, freshness and vanilla notes Tannin coating or SIMILIOAK TOASTED Yeast derivatives NEO SWEET g/hl Cold soak at D Yeasting (D+ if cold soak),3-,4 g/l Insufficient maturity. Protection of color and aroma, toasted notes, sweetness, erases vegetable character T <15 C / 3 j Compound based on yeast derivatives, rich in polysaccharides VIALATTE FERM R96 g/hl Active dry yeast Coating of hard tannins and increased volume on the palate Extraction of anthocyanins, tannins and aromas Develops complex, spicy berry aromas T AF = 5 C then <5 C when d< 1 Nutrition density 3 pts -> mid-af NUTRICELL MIDFERM g/hl Compound based on mineral nitrogen and yeast derivatives Ensures the proper conducting of the end of the AF, oxygenates after addition to reinforce cell walls Prevents AF stoppages and reactivates AF Tannin add density 15 pts SUBLIRED 1 g/hl Protects oxygen, increased mid-palate and reinforced berry aromas AF Extraction (d>1) d> 14 pumping over d< 14 pumping over Or punching down of cap before mid-af 1 vol / d in doses + O.5 vol / d in two doses (O ) Proanthocyanidic tannins Limits extraction to conserve freshness, fruit and color Avoids acetic souring μoxygenation O 1-4 weeks To be managed based on the target product and wine structure Devatting Aeration -6 ml/l/month O (g) Separation of lees and free-run/press wines Possible additional racking (+ 48h) Racking of press wines to avoid herbaceous tastes MLF Inoculation (optional) REFLEX MALO 36 Kit dose/hl Oenococcus oeni Works in difficult conditions (low ph, high ABV). Guarantees optimum fermentation and organoleptic results MATURING μoxygenation O 1-3 ml/l/month O (g) Depending on taste FINING PROVGREEN PURE WINE 5-15 g/hl Pea proteins Flocculation of suspended particles, eliminates aggressiveness of tannins, brings roundness and suppleness

www.martinvialatte.com SAS SOFRALAB - 79, av. A.A. Thévenet - CS 1131-5153 MAGENTA - France - Phone: + 33 3 6 51 9 3 - Fax: + 33 3 6 51 87 6