CMC-se News. Pavel Kessler. UM Karlsruhe 2016
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1 CMC-se News Pavel Kessler UM Karlsruhe 2016
2 CMC-se Assisted Structure Elucidation and Verification Acquired Data Structure Verification Structure Elucidation Populates Table Table Refinement
3 CMC-se 2.5 Structure Elucidation/Verification PROTON HSQC HMBC Required + any additional spectra as C13 COSY N15 HSQC/HMBC H2BC, ADEQUATE Optional
4 CMC-se 2.5 Assisted Structure Elucidation and Verification Easy of use Support for bigger molecules Performance improvements Simplified fragment handling Handling of underdetermined structures Use on-the-fly ranking Improved data analysis Seamless integration of additional data in existing project General usage of charged atom states Functional groups Inner salts Verification Use partial assignment
5 CMC-se Workflow
6 CMC-se Workflow Analysis
7 CMC-se Workflow Interactive data inspection
8 CMC-se Workflow Structure Generation
9 CMC-se Workflow Inspect the results
10 CMC-se Workflow Additional input
11 CMC-se 2.5 News Easy of use Generated structures may be edited Support for ROESY experiments Interactive definition of relative stereochemistry (Up/Downs) Display of correlations reworked Allows to build up the structure manually Interface to CSEARCH Robot Referee Structure Assignment Check Dereplication
12 CMC-se 2.5 News Generated structures may be edited Add explicit proton (s) Refine the structure Bruker Corporation 11
13 CMC-se 2.5 News Support for ROESY experiments ROESY/COSY correlations connect the respective protons COSY Thru space ROESY Bruker Corporation 12
14 CMC-se 2.5 News Support for ROESY experiments Define the relative stereochemistry Bruker Corporation 13
15 CMC-se 2.5 News Display of correlations reworked Selection of visible correlations Bruker Corporation 14
16 CMC-se 2.5 News Display of correlations reworked Correlations shown in fragment editor Build up the structure manually 5 Oxygen still missing Bruker Corporation 15
17 CMC-se in the practice From a Diary of an Oenophile Valpolicella Superiore Ripasso Montere DoP Corvina Molinara Rondinella Corvinone Rossignola Negrara, Barbera, Sangiovese Bigolona Sensoric analysis Primitivo!? Bruker Corporation 16
18 PCA of LC-MS data obtained from SPE extracts corvina m/z, retention time ripasso primitivo
19 Non-targeted time slice-spe-nmr Injection: 5x10uL -> 55 SPE extracts in CD 3 OD corvina Cartridge E4 primitivo
20 Corvina vs. Primitivo
21 Aromatic region Cartridge E4 quercetin OH HO O OH OH OH O
22 NMR spectra of corvina and primitivo marker after isolation and cleaning
23 Structure elucidation using CMC-se: Corvina
24 Structure elucidation using CMC-se: Primitivo
25 Extracted rows from time slice OH HO O OH OH OH O m/z 477? OH O OH HO O O OH OH
26 CMC-se in the practice From a Diary of an Oenophile Unknown substance C21H18O13 30 HMBC + 3 COSY 29 Unique correlations (21+18)/29 = 0.74 correlation / atom Bruker Corporation 25
27 CMC-se in the practice From a Diary of an Oenophile Unknown substance 1) Try to identify it Bruker Corporation 26
28 CMC-se in the practice From a Diary of an Oenophile Unknown substance 1) Try to identify it Bruker Corporation 27
29 CMC-se in the practice From a Diary of an Oenophile Unknown substance 1) Try to identify it 2) Verify it in CMC-se Bruker Corporation 28
30 CMC-se in the practice From a Diary of an Oenophile Conclusions NMR is the method to detect falsified or wrong declared wines. SPE NMR and CMC-se are appropriate tools for the identification and elucidation of components in wines Miquelianin identified as a marker for Corvina wines It exhibits antioxidant and antidepressant activities It is able to reach the CNS after oral administration Makes red wine happy? Bruker Corporation 29
31 Innovation with Integrity Acknowledgments: Markus Godejohann, Bruker Copyright 2011 Bruker Corporation. All rights reserved.
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