Supplemental Data. Ginglinger et al. Plant Cell. (2013) /tpc

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1 -3. 1:1 3. At4g1673 At4g1674 At2g2421 At1g6168 At3g2581 At3g533 At1g137 At3g4425 At2g4558 At3g157 At4g3948 At4g3949 At5g4462 At3g5313 At3g2583 or At3g2582 At5g4259 At4g1331 At4g1329 At3g1468 At4g3741 At5g5886 At5g4799 At2g14 At1g556 At5g48 At5g3614 TPS2 TPS3 TPS1 TPS14 TPS24 CYP71B31 CYP71B27 CYP71B38 CYP76C3 CYP77A6 CYP96A9 CYP96A1 CYP76A3 CYP97C1 TPS23 or 27 CYP71A16 CYP71A2/A19 CYP72A14 CYP81F4 CYP86A1 CYP75A5 CYP75A13 CYP75A25 CYP78A2 CYP716A2 suspension.cell..1d..19. suspension.cell..3d..19. suspension.cell..5d..19. suspension.cell..7d..19. suspension.cell..h suspension.cell..3h suspension.cell..6h suspension.cell..12h suspension.cell..24h suspension.cell.12.c suspension.cell.17.c suspension.cell..22.c suspension.cell..27.c suspension.cell..dark suspension.cell..light suspension.cell..senescing..26. suspension.cell..aphidicolin..h...5. suspension.cell..aphidicolin..2h..5. suspension.cell..aphidicolin..4h..5. suspension.cell..aphidicolin..6h..5. suspension.cell..aphidicolin..8h..5. suspension.cell..aphidicolin..1h..5. suspension.cell..aphidicolin..12h..5. suspension.cell..aphidicolin..14h..5. suspension.cell..aphidicolin..16h..5. suspension.cell..aphidicolin..19h..5. suspension.cell..sucrose..h..6. suspension.cell..sucrose..2h..6. suspension.cell..sucrose..4h..6. suspension.cell..sucrose..6h..6. suspension.cell..sucrose..8h..6. suspension.cell..sucrose..1h..6. suspension.cell..sucrose..12h..6. suspension.cells..xylogenesis..brassinolide.and.h3bo3..od suspension.cells..xylogenesis..brassinolide.and.h3bo3..2d suspension.cells..xylogenesis..brassinolide.and.h3bo3..4d suspension.cells..xylogenesis..brassinolide.and.h3bo3..6d suspension.cells..xylogenesis..brassinolide.and.h3bo3..8d suspension.cells..xylogenesis..brassinolide.and.h3bo3..1d suspension.cell..h..1. suspension.cell..ler..26. protoplast..62. callus..transdifferentiation.a callus..transdifferentiation.a callus..transdifferentiation.a callus..transdifferentiation.a callus..transdifferentiation.a callus..transdifferentiation.a seedlings..post.germination seedlings..2d..28. seedlings..3d..sucrose..63. seedlings..3d..no.sucrose..63. seedings..7d..no.sugars..15. seedings..7d..no.sugars..14. seedlings..7d..short.day seedlings..7d.22.c..then.3d.12.c seedlings..7d.22.c..then.3d.17.c seedlings..7d.22.c..then.3d.22.c seedlings..7d.22.c..then.3d.27.c seedlings..1d..ler seedlings..1d.ms.plate..3d.ms.liquid..col..13. seedlings..1d.ms.plate..4d.ms.liquid..col..13. seedlings..true.leaves.emerged..29.c..55. seedlings..14d..25. seedling..59. seedling..75. seedling..81. seedling..87. seedling..93. seedling..98. seedling..99. seedling seedling..14. seedling seedling seedling seedling seedling seedling..cont..light..26h seedling..cont..light..26h seedling..cont..light..34h seedling..cont..light..38h seedling..cont..light..42h seedling..cont..light..46h seedling..cont..light..5h seedling..cont..light..54h seedling..cont..light..58h seedling..cont..light..62h seedling..cont..light..66h seedling..cont..light..7h seedling..cont..light..74h seedling..far.red.light..2h seedling..far.red.light..6h seedling..far.red.light..1h seedling..far.red.light..14h seedling..far.red.light..18h seedling..far.red.light..22h seedling..far.red.light..26h seedling..far.red.light..3h seedling..far.red.light..34h seedling..far.red.light..38h seedling..far.red.light..42h seedling..far.red.light..46h seedling..far.red.light..5h seedling..white.light.2h..24h seedling..white.light.2h..26h seedling..white.light.2h..28h seedling..white.light.2h..3h seedling..white.light.2h..32h seedling..white.light.2h..34h seedling..white.light.2h..36h seedling..white.light.2h..38h seedling..white.light.2h..4h seedling..white.light.2h..42h seedling..white.light.2h..44h seedling..white.light.2h..46h seedling..white.light.2h..48h seedling..white.light.28h..24h seedling..white.light.28h..26h seedling..white.light.28h..28h seedling..white.light.28h..3h seedling..white.light.28h..32h seedling..white.light.28h..34h seedling..white.light.28h..36h seedling..white.light.28h..38h seedling..white.light.28h..4h seedling..white.light.28h..42h seedling..white.light.28h..44h seedling..white.light.28h..46h seedling..white.light.28h..48h seedling..far.red.then.white.light..83. seedling..dark.then.white.light..83. Cotyledon..87. cotyledon Hypocotyl..87. Hypocotyl shoots..16d..12. shoots..16d.and.15min..12. shoots..16d.and.3min..12. shoots..16d.and.1h..12. shoots..16d.and.3h..12. shoots..16d.and.4h..12. shoots..16d.and.6h..12. shoots..16d.and.12h..12. shoots..16d.and.24h..12. leaf..stage leaves..juvenile..86. leaves..juvenile leaves..juvenil..8. leaves..juvenile..48. leaves..youngest.fully.expanded..58. leaves..third.or.fourth.pair..17. leaves..young..86. leaves.1.and leaf.young..88. leaf leaf leaf leaf leaf leaf adult.leaf..c adult.leaf..52. adult.leaf..92. mature.leaf..stage leaves..adult..61. leaves..adult..82. leaves..mature leaves..12.week..short.day..then.long.day.until.bolting leaves..rosette shoots d..just.started.to.bolt leaves..juvenil.adult..27. whole.rosette..col..12. rosette..young..88. rosette..young rosette..young rosette..7d..91. rosette..14d..91. rosette..21d..91. rosette..ws rosette..col rosette..cal rosette..col rosette..young..85. rosette..93. rosette..95. rosette..97. leaf..whole.rosette leaf..whole.rosette..h..56. leaf..whole.rosette..1h..56. leaf..whole.rosette..2h..56. leaf..whole.rosette..4h..56. leaf..whole.rosette..8h..56. leaf..whole.rosette..12h..56. leaf..whole.rosette..13h..56. leaf..whole.rosette..14h..56. leaf..whole.rosette..16h..56. leaf..whole.rosette..2h..56. leaf..whole.rosette..24h..56. leaf..whole.rosette..h leaf..whole.rosette..4h leaf..whole.rosette..8h leaf..whole.rosette..12h leaf..whole.rosette..16h leaf..whole.rosette..2h leaf..mature..high.light..68. leaf..mature..low.light..68. leaf..petiole..13. leaf.stage.7..petiol..88. leaf.stage.7..proximal.half..88. leaf.stage.7..distal.half..88. guard.cell.enriched..11. cauline.leaf..88. senescing.leaf..col5..6. senescing.leaf..col..6. senescing.leaf..88. senescing.rosette..low.nitrogen..no.glucose senescing.rosette..low.nitrogen..glucose senescing.rosette..high.nitrogen..glucose leaf..dedifferentiation.a1..5. leaf..dedifferentiation.a2..5. leaf..dedifferentiation.a leaf..dedifferentiation.a leaf..dedifferentiation.a leaf..dedifferentiation.a leaf..dedifferentiation.a leaf..dedifferentiation.a leaf..dedifferentiation.b1..5. leaf..dedifferentiation.b2..5. roots..16d..12. roots..16d.and.15min..12. roots..16d.and.3min..12. roots..16d.and.1h..12. roots..16d.and.3h..12. roots..16d.and.4h..12. roots..16d.and.6h..12. roots..16d.and.12h..12. roots..16d.and.24h..12. roots..from.seedlings roots..87. roots..88. roots roots roots roots..92. root..ws Roots..N3.depleted..64. Roots..N3.depleted..dex.treated..64. roots..4.weeks.hydroponics mature.roots..23. root.elongation.zone..79. root..primary.hair.root.elongation.zone..29. roots..axillary.buds..dormant..3. roots..axillary.buds..active..3. lateral.roots..24. root..endodermis root..lateral.cap root..stage.i.root.tip root..stage.ii.elongation.zone root..stage.iii.hair.zone root.stele root.endodermis...cortex root.epidermis.atrichoblasts hypocotyl..mature.plants hypocotyl..mature.plants..col hypocotyl..mature.plants..ler secondary.hypocotyl..xylem secondary.hypocotyl..cork stem..base..injured.with.needle..8. stem..8. stem stem..88. stem..1st.node..88. stem..tip..66. stem..base..66. stem..tip stem..upper.middle.part stem..lower.middle.part stem..base shoot.apex..87. shoot.apex shoot.apex..88. shoot.apex shoot.apex.and.juvenile.leaf apical.region..vegetative..col..94. apical.region..reproductive..3d..col..94. apical.region..reproductive..5d..col..94. apical.region..reproductive..7d..col..94. apical.region..vegetative..ler..94. apical.region..reproductive..3d..ler..94. apical.region..reproductive..5d..ler..94. apical.region..reproductive..7d..ler..94. shoot.apex...days shoot.apex..3.days shoot.apex..5.days shoot.apex..7.days Inflorescence..empty.vector flower.silique..stage.i..84. flower.silique..stage.ii..84. flower.silique..stage.iii..84. flower.bud..young..9. flower.bud..old..9. flower..8. flower..92. flower... flowers..empty.vector flowers..empty.vector flowers flower.buds flowers..stage col flowers..stage col flowers..stage ler flowers..stage ler flowers..stage flower..stage annot..as.shoot.apex.ap1.15. flower..stage annot..as.shoot.apex.ap1.15. flower..stage flower..stage sepal..stage sepal..stage petal..stage petal..stage pedicel..stage stamen..stage stamen..stage pollen..uninucleate.microspores..22. pollen..bicellular..22. pollen..tricellular..22. pollen..mature..22. pollen..74. pollen..hydrated.grains carpel..stage carpel..stage stigma..ler ovary..ler ovule..unfertilized seeds..24h.post.fertilization seeds..cotyledon.stage seeds..mature..green seeds..post.mature..green siliques silique..young silique..old silique..stage silique..stage silique..stage seed..stage seed..stage seed..stage seed..stage seed..stage seed..dry seed..dry seed..fresh..96. seed..dormant..96. seeds..stratified.at.4.c..2d seed..imbibed..1h seed..imbibed..3h seed..imbibed..22.c seed..imbibed seeds..imbibed..3h seeds..imbibed...9h seeds..imbibed..6h seed..imbibed..far.red..22.c seed..imbibed..4.c seed..imbibed..far.red..4.c Supplemental Figure 1: Expression heatmap of Arabidopsis thaliana monoterpene synthases and co-regulated cytochromes P45 with detailed annotation. Values are based on background corrected Affymetrix microarray data. Color intensity in yellow (positive) or blue (negative) is proportional to log2 ratio compared to the mean intensity of all organs and tissues. Number in columns refers to Genevestigator experiment identifier ( 1

A B C D E F Supplemental Figure 2: Confocal microscopy showing

CYP76C3:eGFP and CYP71B31:eGFP ER were transiently expressed in

mrfp:hdel and visualized by confocal microscopy.

2 A B C D E F Supplemental Figure 2: Confocal microscopy showing co-localization of CYP76C3 and CYP71B31 with an ER marker. CYP76C3:eGFP and CYP71B31:eGFP ER were transiently expressed in N. benthamiana epidermal cells together with the ER marker mrfp:hdel and visualized by confocal microscopy. (A) CYP76C3:eGFP, (D) CYP71B31:eGFP, (B and E) mrfp:hdel, (C and F) merged. Scale bar: 1 µm. 2

A B C D Supplemental Figure 3: Confocal microscopy of

(B and D) merged with chloroplast autofluorescence.

3 A B C D Supplemental Figure 3: Confocal microscopy of CYP71B31:eGFP showing ER membranes encircling the chloroplasts. Chloroplasts (chlorophyll autofluorescence in plastids stroma) in red. (A and C) CYP71B31:eGFP localized on ER membranes. (B and D) merged with chloroplast autofluorescence. Scale bar: 1 µm. Arrows (A and C) point to cavities formed by ER in which chloroplasts fit perfectly with region of potential contact between ER and plastid envelope (B and D). 3

A TPS1 1761bp TPS14 171bp RB LB Line 2: Salk_114189 CYP76C3 1548bp CYP71B31 1497bp 1 2 3 4 5 6 LB RB Line 2: Salk_41114 1 2 3 4 5 6 LB RB Line 3: Salk_5982 1 2 3 RB LB Line 2: Salk_7733 1 LB RB Line

4 A TPS1 1761bp TPS14 171bp RB LB Line 2: Salk_ CYP76C3 1548bp CYP71B bp LB RB Line 2: Salk_ LB RB Line 3: Salk_ RB LB Line 2: Salk_ LB RB Line 2: Salk_9366 RB LB Line1: Salk_1842 LB RB Line1: Salk_39462 RB LB Line1: Salk_56876 in intron 3 LB RB Line1: Salk_34144 B tps14 null line 3 WT WT tps1 tps14 double null line tps14 line 3 WT tps1 tps14 TPS1 TPS14 TPS14 AtTUB4 Legend: T-DNA position and orientation Introns position AtTUB4 C 1.2 Relative transcripts level wild type null mutants. tps1 line 1 tps1 line 2 cyp76c3 line 1 cyp76c3 line 2 cyp71b31 line 1 cyp71b31 line 2 Supplemental Figure 4: Characteristics and validation of the insertion mutants. (A) Positions of T-DNA in candidate genes for each insertion line tested. (B) RT-PCR on transcripts for insertion mutants tps14 line 3 and double mutant line tps1 tps14. (C) Transcript levels in flowers measured by qrt-pcr for insertion mutants tps1 lines 1 and 2, cyp76c3 lines 1 and 2, and cyp71b31 lines 1 and 2. The T-DNA was not located in TPS14 insertion lines 1 and 2. 4

5 A B Relative abundance C Retention time (min) Supplemental Figure 5: Linalool emission is absent from flowers of double mutant tps1 tps14. The emitted volatiles of flower bouquets (~6 inflorescences) of wild type and double mutant line tps1 tps14 were collected for 6 hours and analyzed in GC-MS as decribed in Methods. (A) Linalool reference, (B) double mutant tps1 tps14, (C) wild type. Linalool emission was not affected in single mutant lines tps1 and tps14 (data not shown). 5

6 Supplemental Figure 6: Linalool emitted by Arabidopsis flowers from the P45 insertion mutants. A small but significant increase of linalool emission is observed with the flowers of cyp76c3 insertion line. Error bars: SE, n=3. *, p <.5. 6

7 References Additional substructures detected RT (min) Mass [M+H] + [M+H-H 2 O] + [M+H-2H 2 O] + mass 1 formula 1 mass 2 formula 2 mass 3 formula 3 mass 4 formula 4 linalool C1H17O C1H C9H C8H11 1,2 epoxylinalool C9H C7H11 8-hydroxylinalool C8H C8H C7H9 8-oxo-linalool C9H C8H C7H C7H9 lilac alcool C9H15 lilac alcool epoxide C9H15 Compound 1 - RT: 4.52 min Compound 2 - RT: 6.38 min 8-carboxy-dihydro-linalool C1H18O C1H18O hydroxy-8-oxo-linalool lilac alcool epoxy- 1,2- or 6,7- epoxy-linalool epoxy- epoxy- + hexose (+C6H12O6 - H2O) C16H28O hexose (+C6H12O6 - H2O) C16H28O C9H C7H C1H C7H C8H Compound 3 - RT: 7.4 min Compound 4 - RT: 7.44 min C1H18O C1H18O ,2- or 6,7- epoxy-linalool lilac alcool + malonyl hexose (+C9H16O1-2H2O) C19H3O hexose (+C6H12O6 - H2O) C16H28O malonyl glucose-h2o C9H12O Compound 5 - RT: 7.56 min Compound 6 - RT: 7.6 min C1H18O carboxy-linalool C1H16O lilac alcool hydroxy-oxo-linalool epoxy-lilac aldehyde epoxy-8-oxo-linalool + hexose (+C6H12O6 - H2O) C16H28O Compound 7 - RT: 7.67 min Compound 8 - RT: 7.92 min C1H18O linalool C1H18O ,2- or 6,7- epoxy-linalool + malonyl hexose (+C9H16O1-2H2O) C19H3O hydroxy-dihydro-linalool C1H2O ,2- or 6,7-epoxy-dihydro-linalool + malonyl hexose (+C9H16O1-2H2O) C19H3O malonyl hexose (+C9H16O1-2H2O) C19H32O malonyl glucose-h 2O C9H12O malonyl glucose-h2o C9H12O Compound 9 - RT: 8.48 min Compound 1 - RT: 8.6 min C1H18O hydroxy-dihydro-linalool C1H2O lilac alcool 1,2- or 6,7-epoxy-dihydro-linalool C7H malonyl hexose (+C9H16O1-2H2O) C19H3O Compound 11 - RT: 8.62 min Compound 12 - RT: 8.85 min C1H18O hydroxy-dihydro-linalool C1H2O ,2- or 6,7- epoxy-linalool 1,2- or 6,7-epoxy-dihydro-linalool C7H malonyl hexose (+C9H16O1-2H2O) C19H3O Compound 13 - RT: 1.25 min Possible structure Brut formula Mass [M+H] + [M+H-H 2 O] + [M+H-2H 2 O] + C1H18O ,2- or 6,7- epoxy-linalool Supplemental Figure 7: Linalool derivatives detected in UHPLC-Orbitrap analysis. Top lists expected masses of reference compounds, dehydrated forms and derived fragments in ESI+ mode. Bottom lists detected masses of 13 linalool derivatives found in Arabidopsis flower methanol extracts and respective putative identification. 7

8 Incubation product 1 4- identified by NMR Incubation product 5 8-oxo-linalool or OH Incubation product 2 1,2-epoxy-linalool Reference: 8-oxo-linalool O O Reference: 1,2-epoxy-linalool Reference: 9- OH Incubation product 3 5- identified by NMR OH Incubation product Incubation product 4 5- identified by NMR OH Reference: 8- OH m/z Supplemental Figure 8: GC-MS mass spectra of the linalool conversion products and references. Compounds were identified by comparison with authentic references (shown on figure) or by NMR. 8

9 Genotyping of Arabidopsis null mutant lines PCR with T-DNA Left Border Insertion line primer 1 primer 2 TPS1 line 1 GCGTGGACCGCTTGCTGCAACT (LBb1) CGTTGTCCGCAAAAAACGTTTCTTAATTG TPS1 line 2 GCGTGGACCGCTTGCTGCAACT (LBb1) CGATCACAATATTGTGCTACAAGTG TPS14 line 1 GCGTGGACCGCTTGCTGCAACT (LBb1) GATGTGGAGAAGGCAAGAAGCCAGCCG TPS14 line 2 GCGTGGACCGCTTGCTGCAACT (LBb1) CCATTCTGGATCTTCTTGACCCGCG TPS14 line 3 GCGTGGACCGCTTGCTGCAACT (LBb1) GGTTCTTCACGTTCTTCGGATTTGCTTCC CYP76C3 line 1 GCGTGGACCGCTTGCTGCAACT (LBb1) AGAGCCATCGAGATTCCCGGAC CYP76C3 line 2 GCGTGGACCGCTTGCTGCAACT (LBb1) AGAGCCATCGAGATTCCCGGAC CYP71B31 line 1 GCGTGGACCGCTTGCTGCAACT (LBb1) CTTCCTTGTGAAGTGGTTTGC CYP71B31 line 2 GCGTGGACCGCTTGCTGCAACT (LBb1) GATGTGCCTCGACAGAGTTGCC PCR over insertion locus Insertion line primer 1 primer 2 TPS1 line 1 CGTGATCCCATTCCTCAAAAAATC CGTTGTCCGCAAAAAACGTTTCTTAATTG TPS1 line 2 GAGTTCCTCTTGATGAATAGCTTGTACG CGATCACAATATTGTGCTACAAGTG TPS14 line 1 CGAACTCTGGCGAATGGCGTGG GATGTGGAGAAGGCAAGAAGCCAGCCG TPS14 line 2 GGTTCTTCACGTTCTTCGGATTTGCTTCC CCATTCTGGATCTTCTTGACCCGCG TPS14 line 3 CCATTCTGGATCTTCTTGACCCGCG GGTTCTTCACGTTCTTCGGATTTGCTTCC CYP76C3 line 1 TGAAGCTTGGAAGGTTAACCGC AGAGCCATCGAGATTCCCGGAC CYP76C3 line 2 TGAAGCTTGGAAGGTTAACCGC AGAGCCATCGAGATTCCCGGAC CYP71B31 line 1 CACTGACTTCTTCCCCAGAGG CTTCCTTGTGAAGTGGTTTGC CYP71B31 line 2 CGACCGGCTCCAAAGGGCAAG GATGTGCCTCGACAGAGTTGCC Amplification of candidate genes Forward primer Reverse primer TPS1 GGCTTAAUATGGCCACTCTCCTGCAA GGTTTAAUTCAATCTAAAGGAATCGGATTG TPS1 without plastid transit GGCTTAAUATGCAGCGTCGTTCTGCG GGTTTAAUTCAATCTAAAGGAATCGGATTG sequence TPS14 GGCTTAAUATGGCCTTAATAGCTACC GGTTTAAUTTACATTAGAGACTTGAGATATTCG TPS14 without plastid transit GGCTTAAUATGATCGATGTCATTCAAAGT GGTTTAAUTTACATTAGAGACTTGAGATATTCG sequence CYP76C3 GGCTTAAUATGGACCTCTCACTAATTCAAGG GGTTTAAUTTAATAAGAAGACGATATTGTAGGTTTC CYP71B31 GGCTTAAUATGTCTATGTTCCTAGGTTTGCTC GGTTTAAUTTATGGAAGAGTTGGTACGAGC Amplification of promoter of candidate genes Prom. TPS1 GGCTTAAUGTTCTCGTGTGATTTGATGATAC GGTTTAAUATTGAATAAATGTATTATTATGCTATACGTAAC Prom. TPS14 GGCTTAAUCACCATTCGATTCAATTTTAAG GGTTTAAUGATGTATGAACTTAAGTTTTGTTTTG Prom. CYP76C3 GGCTTAAUCTGACTCTTATTGTGGTTTTTGTT GGTTTAAUTGGACGACCACTTTTTTCTTTAGG Prom. CYP71B31 GGCTTAAUCAATGATATGGACGATGACAG GGTTTAAUGCAAACCTAGGAATATAGATATAATTATTG Amplification of candidate genes and fusion with egfp or mrfp TPS1 GGCTTAAUATGGCCACTCTCCTGCAA ATGTGGCGACCGGUACCATCTAAAGGAATCGGATTG TPS14 GGCTTAAUATGGCCTTAATAGCTACC ATGTGGCGACCGGUACCCATTAGAGACTTGAGATATTCG CYP76C3 GGCTTAAUATGGACCTCTCACTAATTCAAGG ATGTGGCGACCGGUACCATAAGAAGACGATATTGTAGG CYP71B31 GGCTTAAUATGTCTATGTTCCTAGGTTTGCTC ATGTGGCGACCGGUACCTGGAAGAGTTGGTACGAGC egfp ACCGGTCGCCACAUGGTGAGCAAGGGCGAGG GGTTTAAUTTAGGCCATGATATAGACGTTGTGG mrfp ACCGGTCGCCACAUGGCCTCCTCCGAGGACGTCATC GGTTTAAUTTAGGCGCCGGTGGAGTGGCG qrt-pcr and RT-PCR TPS1 ATCGTACAAGCTATTCATCAAGAGGAACT ACCTAAACCTGTCTCCATCCACC TPS14 GTCATTGACTCAAGGAGAAATGTCTCAAAC GCTTCTTGCCTTCTCCACATCTTT CYP76C3 CCCGGAAACATTGACATGAGCGA ACGATATTGTAGGTTTCTTGACGGGT CYP71B31 CTTACGATCATCTCATAGCAATGATGTCGG ATTGTTACTGTTCCAGCGTTTACTCCC SAND (At2g2839) GGATTTTCAGCTACTCTTCAAGCTA CTGCCTTGACTAAGTTGACACG TIP41 (At4g3427) GAACTGGCTGACAATGGAGTG ATCAACTCTCAGCCAAAATCG PP2A (At1g1332) GACCGGAGCCAACTAGGAC AAAACTTGGTAACTTTTCCAGCA EXP (At4g2641) GAGCTGAAGTGGCTTCCATGA GGTCCGACATACCCATGATCC Supplemental Table 1: Primers used for genotyping of insertion lines, for amplification of ORFs and 1.5 kbpromoter region of candidate genes, and for qrt-pcr or RT-PCR. 9

10 Arabidopsis insertion mutant lines SALK line name Status tps1 line 1 SALK_1842 Homozygous tps1 line 2 SALK_41114 Homozygous TPS14 line 1 SALK_39462 Heterozygous TPS14 line 2 SALK_ Heterozygous tps14 line 3 SALK_5982 Homozygous cyp76c3 line 1 SALK_56876 Homozygous cyp76c3 line 2 SALK_7733 Homozygous cyp71b31 line 1 SALK_34144 Homozygous cyp71b31 line 2 SALK_9366 Homozygous tps1 tps14 Selected after cross fecundation of tps1 line 2 and tps14 line 3 Homozygous Supplemental Table 2: Name and status of employed Arabidopsis insertion mutant lines. 1

Supplemental Data. Jeong et al. (2012). Plant Cell /tpc

Supplemental Data. Jeong et al. (2012). Plant Cell /tpc Suppmemental Figure 1. Alignment of amino acid sequences of Glycine max JAG1 and its homeolog JAG2, At-JAG and NUBBIN from Arabidopsis thaliana, LYRATE from Solanum lycopersicum, and Zm- JAG from Zea mays.