BioSciWARE. Scientific Labware

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1 BioSciWARE Scientific Labware

2 BioSciWARE Tissue Grinders Wheaton Tissue Grinders enable primary cells to be prepared from excised tissues, as well as subcellular components from harvested cell packs. Tissue grinders are used to extract DNA, RNA and proteins for blots or other procedures. They are also used to enhance recovery of other cell products. Capacities on the grinders range from 0.1 ml to 55 ml. There are a variety of grinders to suit many tissue types. Tapered Tissue Grinders are ideal for connective tissue including heart, muscle and lung. These glass-on-glass grinders feature a long taper design that maximizes grinding efficiency. Potter-Elvehjem Tissue Grinders incorporate a serrated pestle design to disperse homogenate into the mortar cylinder. Standard units with Teflon pestles are well suited for soft tissue, such as brain and liver. Tenbroeck Tissue Grinders have a hollow handle that allows packing with ice, making them suitable for tissue such as liver, intestines and heart. Dounce Tissue Grinders are supplied with two types of pestles: a loose-fitting pestle for sample reduction and a second, tighter pestle for complete homogenization. The ball-shaped pestles leave cell nuclei intact in soft tissues. The Wheaton Micro Tissue Grinder Kit offers a complete selection of micro tissue grinders. All Wheaton tissue grinders are manufactured from Wheaton 33 low extractable borosilicate glass that conforms to USP Type I and ASTM E-438 Type I, Class A requirements and replacement parts can be purchased separately. Dounce Tissue Grinder Ideal for soft tissue Provided with loose and tight pestle Wheaton 33 low extractable borosilicate glass x A 7 13 x B x C x Dounce Dura-Grind Tissue Grinder Rugged alternative to glass Dounce Tissue Grinder Precision machined from No. 316 stainless steel Mortar and pestle provided as a matched set Not autoclavable A 7 35 x C x x

3 Tissue Grinders Potter-Elvehjem Tissue Grinder Ideal for tissue homogenates Use with power homogenization Parts interchangeable Teflon Pestle x x A x B x C x D x Potter-Elvehjem Tissue Grinder with Radial Serrations Mortar made from Wheaton 33 low extractable borosilicate glass Radial serrated Teflon pestle Interchangeable parts x x x x x x Potter-Elvehjem Safe-Grind Tissue Grinder Exterior plastic coated glass mortar provide greater safety Ideal for tissue homogenates Mortar made from Wheaton 33 low extractable borosilicate glass PTFE pestle with stainless steel shaft can be used with power homogenization A 2 11 x C 5 13 x x G x J x M x Potter-Elvehjem Micro Tissue Grinder Made from Wheaton 33 low extractable borosilicate glass Micro size for extremely precise work Reservoir and pouring lip are incorporated into the design A x B x

4 Tapered Tissue Grinder Homogenizes connective tissue including heart, muscle and lung Made from Wheaton 33 low extractable borosilicate glass Interchangeable components x x x x Tenbroeck Tissue Grinder Ideal for grinding liver, intestine and heart tissue Made from Wheaton 33 low extractable borosilicate glass Hollow pestle can be packed with ice Pour lip on mortar x A 2 11 x B 7 16 x C x D x Tapered Tissue Grinder Teflon pestle with stainless steel rod fits power homogenizer Homogenizes soft tissue such as brain Mortar made from Wheaton 33 low extractable borosilicate glass Interchangeable components x x x x Disposable Micro Tissue Grinder Disposable tissue grinder eliminates cross contamination Useful for radioactive samples Small grinder area allows for high sample recovery Made from Wheaton 33 low extractable borosilicate glass Cat. No. Size (ml) ID x Length (mm) Overall Length (mm) Qty/Case x

5 Tissue Grinders Roller Bottles Reusable Strong borosilicate glass to withstand repeated wet or dry sterilization 38, 45 or 51 mm screw thread Micro Tissue Grinder Kit Made from Wheaton 33 low extractable borosilicate glass Complete kit includes 7 micro tissue grinders Replacement parts available separately Complete Kit 1 Kit Replacement Parts Cat. No. Description Qty/Kit Qty/Case ml Tenbroeck Tissue Grinder ml Tissue Grinder, G.P.I cap ml Dounce Tissue Grinder A 0.1 ml Tissue Grinder B 0.2 ml Tissue Grinder ml Potter-Elvehjem Tissue Grinder ml Tapered Tissue Grinder 1 1 Dia x Flat Overall Usable Approx Cat. No. Length (mm) Surface Area (cm 2 ) Surface (cm 2 ) Capacity (ml) Qty/Case Roller bottle with 38 mm deep skirted, black phenolic screw cap with white styrene-butadiene rubber liner (G.P.I ) A 110 x x x D 110 x Roller bottle with 45 mm white polypropylene screw cap with inner sealing ring, and polypropylene pour ring. This bottle can also be used with the Vented Cap (Cat. No ) A 110 x B 110 x Roller bottle with 51 mm black phenolic screw cap with shallow skirt and white styrene-butadiene rubber liner (G.P.I ) C 110 x x x

6 Trypsinizing Flasks W heaton trypsinizing flasks are used to further reduce tissue to cellular components. Place tissue in flask, add trypsin and stir the vessel using the Wheaton Micro-Stir Magnetic Stirrer. The deep-fluted flask design promotes mixing turbulence; a dam below the sidearm retains untrypsinized tissue when decanting. Dissociated cells can then be collected. Trypsinizing flasks are also useful when removing cells from microcarrier beads. Single cells can then be used to start a seed culture in a flask or multiple flasks. After reaching a specific density, cultures can seed in a suspension culture flask or roller bottle for high volume cell production. Trypsinizing Flask Fluted design improves mixing ml size Graduated and non-graduated Non-Graduated Trypsinizing Flask With Pourout and Caps Fluted design improves mixing Deep skirted screw caps ml size Graduted and non-graduated Graduated Non-Graduated Non-Graduated Cat. No. Capacity (ml) Cap Size Flask (ml) Qty/Case A B C D Graduated Graduated Cat. No. Capacity (ml) Cap Size Graduations (ml) Flask (ml) Qty/Case A Every B , 25, 50, C , 75, 100, D , 100, 150, Every E Every Non-Graduated Cat. No. Capacity (ml) Top Screw Cap Size Flask (ml) Qty/Case A B C D E F Graduated Cat. No. Capacity (ml) Top Screw Cap Size Graduations (ml) Flask (ml) Qty/Case A Every B , 25, 50, C , 75, 100, D , 100, 150, E Every F Every

7 Trypsinizing Flasks Shake Flask Maximizes aeration for improved growth Break resistant lip Nephelo Culture Flask For use with colorimeter or spectrophotometer Scratch and striation free side tube Cat. No. Capacity (ml) Sidearm Size (mm) Qty/Case A x Nephelo Culture Flask With Cleanout Port and Caps For use with colorimeter or spectro photometer Scratch and striation free side tube Three baffle flask Four baffle flask Cat. No. Capacity (ml) Qty/Case With Three Baffles A B C D E F With Four Baffles A B C E F Erlenmeyer Flask Ideal for media storage and cell culture Made from Wheaton 33 low extractable borosilicate glass Cat. No. Capacity (ml) Volume (ml) Sidearm Size (mm) Qty/Case A x x x x x Cat. No. Capacity (ml) Cap Size Qty/Case

8 Spinner Flasks heaton Science Products has long been an innovator in W cell culture flasks. The Wheaton Magna Flex impeller produces high-yield cultures by maintaining even circulation while keeping cultures in suspension. Slow acceleration and slow stop from the Wheaton Micro-Stir Magnetic Stirrers prevent cell detachment from microcarriers. Wheaton also invented an adjustable impeller flask that does not have a rod extending through the top of a flask. Celstir flasks feature adjustable blade impeller for increased aeration and dimpled bottom to prevent the accumulation of cells under the impeller. When used with the Micro-Stir, which can operate at very low speeds, the Celstir flask provides an optimum environment for most suspension lines, including insect cell cultures. Hybridomas and adapted cell lines can also be grown in these spinner flasks. Sidearms on Magna Flex and Celstir vessels 500 ml and larger were widened to accommodate monitoring and control devices or media inlet/outlet lines. For more information on the line Wheaton Magnetic Stirrers, see catalog numbers , , , and Double Sidearm Celstir Spinner Flask Impeller rod does not extend through the top of cap Sidearms for 500 ml - 8 L flask allow vented caps which enables the flask to breathe while protecting from contamination. 1:1 headspace for good gas exchange No contamination from stirring rod Completely autoclavable Cat. No. Volume (ml) Dia x H (mm) Cap Size, Top Cap Size, Sidearm Qty/Case A x B x C x D x E x mm J x mm F x mm G x mm H x mm 1 7

9 Spinner Flasks Jacketed Double Sidearm Celstir Spinner Flask Water jacket allows for precise temperature control Manufactured from Wheaton 33 low extractable borosilicate glass Proportioned to provide a head space ratio of 1:1 or greater Impeller rod does not extend through the top of cap Fully autoclavable PTFE faced rubber-lined caps on sidearms Cat. No. Volume (ml) Dia x H (mm) Cap Size, Top Cap Size, Sidearm Qty/Case A x B x C x D x E x mm F x mm 1 Magna Flex Microcarrier Spinner Flask Designed for use with microcarrier cultures Side arms for 500 ml - 6 L flasks allow vented caps which enables the flask to breath while protecting from contamination Gentle, even stirring action Cat. No. Volume (ml) Dia x H (mm) Cap Size, Top Cap Size, Sidearm Qty/Case A x B x C x mm D x mm E x mm F x mm 1 Vented Cap, 45 mm Sterile cap with 0.2 μm Gore-Tex filter Flask and bottles with 45 mm screw thread Cat. No. Description Cap Size (mm) Qty/Case Vented Cap Vented Cap

10 Staining/ Mounting/ Microscopy heaton offers a variety of staining dishes and Coplin W jars suitable for various histology staining. Routine staining is performed to give contrast to the tissue being examined, as without staining it is very difficult to see differences in cell morphology. Hematoxylin and eosin (abbreviated H&E) are the most commonly used stains in histology and histopathology. Hematoxylin colors nuclei blue, eosin colors the cytoplasm pink. To see the tissue under a microscope, the sections are stained with one or more pigments. There are hundreds of various techniques which have been used to selectively stain cells and cellular components. Other compounds used to color tissue sections include Safranin, Oil Red O, Congo red, Fast Green FCF, Silver salts and numerous natural and artificial dyes that were usually originated from the development dyes for the textile industry. Wheaton staining dishes, covers, and racks are manufactured from soda-lime glass that resists staining from Eosin or Hematoxylin. Staining dishes are available in many configurations to accommodate up to 50 slides, with various glass and stainless steel racks. Coplin jars can be used for pre-staining with an alcohol buffer. Slides are held vertically for easy manipulation. A Coplin jar can also serve as a preparation chamber for micro thin layer chromatography. Staining Dish, 50-Slide Unit Stainless steel rack holds 50 slides A Complete (Dish, Cover, Rack) Dish Cover C Dish and cover Slide Stainless Steel Rack, with Handle Attached 3 Staining Dish, Slide Unit Holds 10 single slides or 20 slides back to back Staining Dish and Cover 6 Staining Dish, Slide Unit Holds 10 single slides or 20 slides back to back Complete (Dish, Cover, Rack, Handle) Dish Cover A Dish and Cover B Glass Slide Rack C Handle 6 9

11 Staining Dish, Slide Unit Staining Ware Dish accommodates 16, 20 or 30 slide racks Select a glass or stainless steel rack rack holds 16 slides or 32 slides back to back rack holds 20 slides or 40 slides back to back Dish and Cover Dish Cover Slide Rack Stainless Steel with hinged handle (16-32) Slide Rack, Glass (20-40) Slide Rack, Glass 3 Common Histology Stains Stain Common use Nucleus Cytoplasm Red Blood Cell (RBC) Collagen Fibers Specifically stains Hematoxylin General staining Blue N/A N/A N/A Nucleic acids - Blue when paired with Eosin blue eer (ergastoplasm) - Blue Eosin General staining N/A Pink Orange/Red Pink Elastic fibers - Pink when paired with Hematoxylin reticular fibers - Pink Toluidine blue General staining Blue Blue Blue Blue Mast cells granules - Purple Masson s trichrome stain Connective tissue Black Red/Pink Red Blue/Green Cartilage - Blue/green Muscle fibers - Red Mallory s trichrome stain Connective tissue Red Pale Red Orange Deep Blue Keratin - Orange Cartilage - Blue Bone matrix - Deep Blue Muscle fibers - Red Weigert s elastic stain Elastic fibers Blue/Black N/A N/A N/A Elastic fibers - Blue/Black Heidenhains azan Distinguishing cells from Red/Purple Pink Red Blue Muscle fibers - Red trichrome stain extracellular components Cartilage - Blue Bone matrix - Blue Silver stain Reticular fibers, Nerve fibers N/A N/A N/A Brown/Black Reticular & Nerve Fibers - Brown/Black Wright s stain Blood cells Bluish/Purple Bluish/Gray Red/Pink N/A Neutrophil granules - Purple/Pink Eosinophil granules - Bright Red/Orange Basophil granules - Deep Purple/Violet Platelet granules - Red/Purple Orcein stain Elastic fibres Deep Blue N/A Bright Red Pink Elastic fibres - Dark Brown Mast cells granules - Purple Smooth Muscle - Light Blue Periodic (PAS) Basement Membrane, Blue N/A N/A Pink Glycogen and other acid-schiff stain Localising carbohydrates Carbohydrates - Magenta Reference from: Michael H. Ross, Wojciech Pawlina, (2006). Histology: A Text and Atlas. Hagerstown, MD: Lippincott Williams & Wilkins. ISBN

12 Staining Dish, 8-16 Slide Unit Fits 8 single slides or 16 slides backto-back Used as staining dish or TLC developing chamber Includes glass lid Coplin Staining Jar, 5-10 Slide Unit, with Screw Cap Unit holds 5 slides vertically or 10 slides back-to-back Polyproplene screw cap 60 ml capacity Staining Dish E Coplin Jar with Screw Cap Replacement Screw Cap, Columbia Jar for Cover Slips Unit holds 4 cover slips Polyproplene screw cap with PTFE coated PE liner Coplin Staining Jar, 5-10 Slide Unit Holds 10 slides Polypropylene screw cap 55 ml capacity Columbia Jar with Cap Columbia Jar with Cap D Coplin Jar with Screw Cap Replacement Screw Cap,

13 Staining Ware Coplin Staining Jar, 5-10 Slide Unit Fits 5 single slides or 10 slides back-to-back Extra tall lid accommodates 100 mm slides Staining Jar, 8-10 Slide Unit Fits 8 single slides or 16 slides back-to-back Wide top makes transferring slides convenient Includes glass lid Staining Jar with Lid Staining Jar with Lid 6 Coplin Staining Jar, 5-10 Slide Unit Glass Cover 65 ml lid prevents evaporation Fits 5 slides or 10 slides back-to-back Slide Grip Allows for easy and self transfer of slides to Coplin jar Holds 5 slides Coplin Jar with Glass Lid Slide Grip 2 12

14 Cryogenic Vials heaton cryogenic vials, caps, vial racks, and freezer W boxes are designed for simple identification and easy handling. With Wheaton plastic Cryules, both the closures and tubes are manufactured from medical-grade polypropylene with the same coefficient of expansion; this further enhances the leak proof qualities of these vials at changing temperatures. All Wheaton Cryules and racks are autoclavable up to 121 C. When autoclaving plastic Cryules, the cap must be loosened or removed to allow pressure equalization upon cooling; this prevents pressure build-up within the vial. Facts About Cryopreservation Liquid nitrogen (LN ): liquid density at the triple point is g/ 2 ml, is inert, colorless, odorless, non-corrosive, nonflammable, and extremely cold. Liquid nitrogen boils at -196 C, and is a cryogenic fluid which is potentially capable of causing rapid burns on contact with living tissue. Cryopreservation is a process where cells or whole tissues are preserved by cooling to low sub-zero temperatures, such as (typically) 77 K or 196 C (the boiling point of liquid nitrogen). When cryoprotectant solutions are not used, the cells being preserved are often damaged due to freezing during the approach to low temperatures or warming to room temperature. A cryoprotectant is a substance that is used to protect biological tissue from freezing damage (damage due to ice formation). Cryoprotectants act like antifreeze: they lower the freezing temperature. They also increase the viscosity. Instead of crystallizing, the syrupy solution turns into an amorphous ice. Cryule Vial with Colored Caps Cryule Cryogenic Vial, Freestanding Sterile External threads 2 ml size Supplied with colored coded caps Conical bottom Fits Rack Cat. No S Cat. No. Cap Color Size (ml) Dia x H (mm) Qty/Pack Qty/Case D White 2 12 x E Red 2 12 x F Pink 2 12 x G Yellow 2 12 x H Green 2 12 x J Blue 2 12 x Cryule Cryogenic Vial, Freestanding Sterile Internal threads Silicone ring sealing system 1.2, 2 and 4 ml size Cat. No. Size (ml) Dia x H (mm) Qty/Pack Qty/Case N x P 2 12 x Q 4 12 x

15 Cryogenic Vials Cryule Vial, Round Bottom Sterile Internal threads Round bottom 2, 4 and 5 ml size Color code cap inserts available separately Cryule Vial Cap Inserts Color coded for sample and lot identification Cat. No. Size (ml) Dia x H (mm) Qty/Pack Qty/Case K 2 12 x L 4 12 x M 5 12 x Cryule Vial, Round Bottom Sterile External threads Round bottom 1 and 2 ml size White screw cap Cat. No. Color Qty/Case Red Blue Green Yellow White Assorted (100 of each color) 500 Cryule Vial Freezer Box For 1 and 2 ml Cryule Vials Holds 81 Vials Cat. No. Size (ml) Dia x H (mm) Qty/Pack Qty/Case B 1 12 x C 2 12 x Cryule Vial, Round Bottom Non-sterile External thread Round bottom 2 ml size Screw cap included Cat. No Fits Dimensions (L x W x H) Qty/Case ml Cryule Vials 5-1/4 x 5-1/4 x 1-1/4 in (13.3 x 13.3 x 3.1 cm) ml Cryule Vials 5-1/4 x 5-1/4 x 1-3/4 in (13.3 x 13.3 x 4.4 cm) 15 Cryule Vial Rack For use with freestanding Cryule Vials Allows for one-handed cap removal Holds 50 vials Polypropylene Cat. No. Description Dia x H (mm) Qty/Case ml Cryule Vial with Marking Area 12 x A 2 ml Cryule Vial without Marking Area 12 x Cat. No. Well ID Dimensions (L x W x H) Qty/Case S 12.5 mm 7-5/8 x 4 x 7/8 in (19 x 10 x 2.2 cm) 5 14

16 Manufactured by: BioSciWARE, Dura-Grind, Safe-Grind, Celstir, Magna Flex, Cryule and the stylized W are registered trademarks of Wheaton Industries Inc. Gore-Tex is a registered trade mark of Gore and Associates. Teflon is a registered trade mark of E.I. dupont de Nemours Copyright, Wheaton Industries Inc. BN Lit. No /09

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