(75, 50, 25) (15, 10, 5) A Study of the Effect of Thermal Treatments "Hot Water & Microwaves" on Contaminant Fungi of some Stimulating Drinks

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1 ) (7, 0, ) (, 0, ) 0 7 (80, 0) 60 (0, 0) A Study of the Effect of Thermal Treatments "Hot Water & Microwaves" on Contaminant Fungi of some Stimulating Drinks Yussra A. M. Al-rawi Asst. Prof. Dr. Saleh E. M. EL-Gibury Department of Biology College of Science / Mosul University Abstract: The effect of hot water on viability of fungi contaminate for both black tea (Lepton_yellow label) and coffee (Bun Al-amid) at thermal degree (, 0, 7) c of exposing periods (, 0, ) minutes, showed that growth of contaminated fungi had been occurred for both sampleson both and 0 c for all exposing periods, but no growth of fungi on 7 c.

2 Treatment ofthe above samples with microwaves with doses (0, 80, 60) watt with exposing periods (0, 0, ) seconds showed that fungal growth had been occurred on both samples, expect the dose (60) watt for seconds which showed no fungal growth of the two samples also. Hyphea Mycelium Mycetea,Dehoog& Guiro Saprophytes Kown_chung& Bennett, Parasites Pitt & MoGinnis, 80 Colleeet al.,6 Hocking, Theacae

3 Poly phenol Namitaet al., Evans, Rubiaceae 7 Stimulating drink Abdel_satar Hasan 0 Eutrtium Aspergillus,Penicillium,Gladosporium. A (004) Suarez_Quiroz A.ngier A.ochraceus Noonimet al., Aspergillus 7-77) Aspergillus, Penicillium, Fusarium, Mucor, 0 Mohammed (Aly&Bokhari, 00) Rhizopus,Alternaria),A.parasiticus, A. niger A.ochraceusAlternaria, Penicillium 0 Microwaves ) 00

4 B, B, Iha&Truckess, , ) - 00, A 0 G, G) 0.6) Viswanath Samples - Akbar brothers ltd. Akbar brothers ltd. Al- Tunkaya Akbar brothers ltd. Uniliver Uniliver

5 Vretiatrima Preparation of samples Marth, Dilution method 0(Ronald et al., ; 78 Grinder Syringe Potato Dextrose Agar(PDA) 7 6) Raper&Fenel Smith, 7 ;; Konemanet al., 7 ;7Pitt & Hocking

6 Hot water 7, 00 ( 0) 000 (, 0, ) (00, 7, 0, ) (PDA) Potato Dextrose Agar Pitt & Hocking, 7 Microwaves 7, 00) 0 60, ( 000, 0,0) 80, 0 Microwaves Microwaves TERMIKEL PDA Potato Dextrose Agar Pitt & Hocking, 7)

7 8, 0, 7,, 4,,, R.stolonifer 6 penicillium,, 8 Alternariaalternata () 8 7 Aspergillusniger Rhizopusstolonifer Penicilium 6 7

8 6 R.stolonifer A. niger Alternariaalternata R.stolonifer , 7, 0, ) 0, 0, (0, 00 7) (0) 0 (00 7) 0

9 0 Aspergillusniger Uniliver R.stolonfer R.stolonifer / 6 /

10 , 0, 0 60, 80, 0 60, A.sp, Penicilliumsp., Cladosporumsp., Botrytis cinerea, (00) David 4 Helminthosporium AspergillusPenicilliumsp., Aurobasidiumsp. 4 4 Aspergillusniger Uniliver 7 Cladosporum sp. Botrytis cinerea 0

11 4 6 A.sp. Cladosporum sp. Halimenthosporum Penicilliumsp,

12 . (000) 4 (000). (6) (6). (7) 4 4 (0) B (0) 4. (8) (0)

13 Bokhari, E. M. and Aly, M. M. (00). Evolution of traditional Meaus of roasting and mycotoxins contaminated coffee beans in Saudi Arabia. Advances in biological research. (-4) : Collee, J. G. ; Fraser, A. G. ; Marmion, B. P. and Simmeus, A. (6). Practical Medical Microbiology. 4 th ed,chur- Chill Livingstone, pp David, E. ; leahu, A. ; OanCea, S. and Mironescu, M. (00). Effect of microwaves on mouldes is dated from Surfaces. Actavniversitatis series E: Food technology, XIV() : 4. dehoog, G. S. and Guiro, J. ().Atlas of Clinical fungi.univ ersitatroviraivirgili, Spain, 70pp. Evans, W. C. (): Pharmacognosy, 4 th ed. Nottingham, U.K, 6 pp. Hassan, H. A. H. and Abdel_satar, M. A. (). Studies on myc- Flora and aflatoxin in regular and decaffeinated black Tea. Journal of Islamic academy of sciences. 6(): 4-. Iha, M. H. and Trucksess, M. w. (00).Aflatoxins and Ochratoxin A in tea Prepared from ueturallycontamina- ted powdered ginger. Food additives and containats. 7(8): Koneman, E. W.; Roberts, G. D. and Wright, S. E. (7).Practical laboratory mycology. The Williams and Willkimugscompany. nd ed. Baltmor, USA., pp. Kwon, Chung, K. J. and Bannatt, J. E. ().Medical mycology. Lea and Febiger, Philadelphia. London, P Marth, E. H. (78).Standards methods for the examination of dairy products.4 th Edition, American public health association 0 Eighteenth street, N. M. Washington.DC. 006, p. 46. McGinnins, M. R. (80). Laboratory Handbook of Medical Mycology. Academic press, p. 66. Mohammed, M. J. (0). Study the pollution by mycoflora and aflatoxin of some ed black tea kindes in Iraq markets. Journal of Tikrit university for agriculture of sciences. (): 6-6. Namita, P. ;Mukesh, R. and Vijay, K. J. (0).Camellia sineusis (Green tea) : A evicu Global Journal of pharmacology. 6(): -. Noounim, P.; Mahakaruchanakul, W.; Frisvad, J. C. and Gamson, R. A. (007). Ecology and taponomy of ochratoxigenim Aspergillus spp.from coffee beans in Thailand. Kaset Sartuniversity, Bangkok, Thailand. Pitt, J. I. and Hocking, A. D. (7).Fungi and food spoilage. nd ed., Academic press, Sydney. P.66. Raper, B. and Fennel, D. I.(6).The genus Aspergillus. Roberts E. Krieger publishing company. New York. USA., 404 pp.

14 Ronald, M. A.; Alfard, E. B. and Lawrence, C. P. (). Laboratory manual of experimental microbiology, Mosby year book, Inc. United states of America. Smith, J. E. (7).Mycotoxins formation analysis and significance. Jhonwiley and sons Itd.Great Britain, 0 pp. Suarez_Quiroz, M. ; Gonzalez -Rios, O. ; Barel, M. ; Guyot, B.; Schorr_Galindo, S.and Couiraud, J. (004).Stady ofochratoxin A producing strains in coffee processing. International journal of food science and technology. : Viswanath, R.; Nanjegowda, D.; Govindegowda, H.; Dattatreya, And Siddappa, V. (0). Aflatoxin determination In black tea (Camellia sinensis) status and develop. ofaprotocal. Journal of food safety. : -

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