Status: Development of Mapping Popula5ons

Transcription

1 Status: Development of Mapping Popula5ons Sco7 E. Schlarbaum, University of Tennessee and Mark V. Coggeshall, University of Missouri

2 Mapping popula+on development for selected species Host Order Species Parent Selected in Hand DNA Collected Paternity Exclusion Complete Established Fagales Northern red oak Quercus rubra Yes 0 Yes Yes 509 Magnoliales Yellow- poplar Liriodendron tulipifera Yes 550+ Yes No 0 Fabales Honey locust Gleditsia triacanthos Yes 0 Yes No 226+ Sapindales Sugar maple Acer saccharum Yes 150+ Yes No 0 Fagales Black walnut Juglans nigra Yes 0 Yes Yes 303 Proteales Sweetgum Liquidambar styraciflua Yes 150+ No NA 0 Lamiales Green ash Fraxinus pennsylvanica Yes 700+ Yes NA 0 Cornales Black gum Nyssa sylva@ca Yes 125+ No NA 0

3 Northern red oak (Q. rubra) mapping parents SM2 SM1 3

4 Northern red oak mapping popula5on A total of 509 full sibs derived from crossing SM1 and SM2 at Purdue University. Open pollinated seeds collected in 2001, 2003, 2007 and Full sibs iden5fied using paternity exclusion (PE). Full sibs established at Purdue and at the UT Ames Planta5on in western TN. 4

5 Northern red oak mapping popula5on In 2013, we started to gra_ all of the 509 NRO full sibs in Missouri. This clonal popula5on will serve as a back- up to seedlings popula5ons previously established and provide addi5onal phenotyping opportuni5es for the purpose of developing the first gene5c linkage map for the species. 5

6 Oak leaf morphology: QTL mapping in Q. rubra WP LW SW NV LL In 2013, we re- propagated 265 Q. rubra full sibs, plus both parents. For each tree, we measured six different leaf morphology traits, using two mature, first flush leaves from three gra_ed ramets. This dataset will allow us to map QTLs associated with leaf morphology traits in Q. rubra for the first 5me. PL 6

7 Oak leaf morphology: QTL mapping in Q. rubra 7

8 Oak leaf morphology: QTL mapping in Quercus robur Source: Gailling QTL analysis of leaf morphological characters in a Quercus robur full- sib family (Q. robur Q. robur ssp. slavonica). Plant Biol 10:

9 Oak flowering: QTL mapping in Q. rubra 9

10 Mapping popula+on development for selected species Host Order Species Parent Selected in Hand DNA Collected Paternity Exclusion Complete Established Fagales Northern red oak Quercus rubra Yes 0 Yes Yes 400+ Magnoliales Yellow- poplar Liriodendron tulipifera Yes 550+ Yes No 0 Fabales Honey locust Gleditsia triacanthos Yes 0 Yes No 226+ Sapindales Sugar maple Acer saccharum Yes 150+ Yes No 0 Fagales Black walnut Juglans nigra Yes 0 Yes Yes 303 Proteales Sweetgum Liquidambar styraciflua Yes 150+ No NA 0 Lamiales Green ash Fraxinus pennsylvanica Yes 700+ Yes NA 0 Cornales Black gum Nyssa sylva@ca Yes 125+ No NA 0

11 Fagales: Black walnut (Juglans nigra) Schessler Sparrow Angiosperms Monocots Amborellales Magnoliales Laurales Proteales Vitales Iden5fica5on of full sibs using paternity exclusion (PE) has been an integral part of the MU breeding program since 2009, resul5ng in the iden5fica5on of 650+ full sibs, including 303 Sparrow x Schessler seedlings (as of summer 2013). Eudicots Core eudiocts Rosids Malphigiales Fabales Rosales Fagales Myrtales Brassicales Malvales Sapindales Cornales Asterids Lamiales

12 Fagales: Black Walnut (Juglans nigra) Mapping Popula5on Established in spring 2011, with addi5onal seedlings planted in spring Total of 303 full sibs successfully established to date. Total of 370 full sibs represented as DNA samples in ND library The size of this mapping popula5on will be increased in 2014 with the addi5on of ~30 full sibs from the Carlson lab. Addi5onal o.p. seeds were collected in September 2012 for future paternity exclusion, if needed. 2012

13 Mapping popula+on development for selected species Host Order Species Parent Selected in Hand DNA Collected Paternity Exclusion Complete Established Fagales Northern red oak Quercus rubra Yes 0 Yes Yes 400+ Magnoliales Yellow- poplar Liriodendron tulipifera Yes 550+ Yes No 0 Fabales Honey locust Gleditsia triacanthos Yes 0 Yes No 226+ Sapindales Sugar maple Acer saccharum Yes 150+ Yes No 0 Fagales Black walnut Juglans nigra Yes 0 Yes Yes 303 Proteales Sweetgum Liquidambar styraciflua Yes 150+ No NA 0 Lamiales Green ash Fraxinus pennsylvanica Yes 700+ Yes NA 0 Cornales Black gum Nyssa sylva@ca Yes 125+ No NA 0

14 Proteales: Sweetgum (Liquidambar styraciflua) Angiosperms Monocots Amborellales Magnoliales Laurales Proteales Vitales Eudicots A new seed- bearing SWG mother tree (MT) was selected in 2011 and seedlings produced in Stress tes5ng at PSU and CU will be conducted in Core eudiocts Rosids Asterids Malphigiales Fabales Rosales Fagales Myrtales Brassicales Malvales Sapindales Cornales Lamiales

15 Mapping popula+on development for selected species Host Order Species Parent Selected in Hand DNA Collected Paternity Exclusion Complete Established Fagales Northern red oak Quercus rubra Yes 0 Yes Yes 400+ Magnoliales Yellow- poplar Liriodendron tulipifera Yes 550+ Yes No 0 Fabales Honey locust Gleditsia triacanthos Yes 0 Yes No 226+ Sapindales Sugar maple Acer saccharum Yes 150+ Yes No 0 Fagales Black walnut Juglans nigra Yes 0 Yes Yes 303 Proteales Sweetgum Liquidambar styraciflua Yes 150+ No NA 0 Lamiales Green ash Fraxinus pennsylvanica Yes 700+ Yes NA 0 Cornales Black gum Nyssa sylva@ca Yes 125+ No NA 0

16 Lamiales: Green ash (Fraxinus pennsylvanica) Angiosperms Monocots Amborellales Magnoliales Laurales Proteales Vitales The development of a GRA mapping popula5on was part of the original NSF proposal related to the current project. However, PE of the 700+ (1+1) seedlings currently available has not been ini5ated due to funding constraints. Eudicots Core eudiocts Rosids Asterids Malphigiales Fabales Rosales Fagales Myrtales Brassicales Malvales Sapindales Cornales Lamiales

17 Lamiales: Green ash (Fraxinus pennsylvanica) In 2012, We completed DNA sampling of the MT to facilitate gene5c marker development at ND. Completed DNA sampling of all puta5ve males for future PE (if funded). Mul5ple 5ssue types were collected from the MT for mrna extrac5on by PSU. Supplied 100 GRA seedlings to CU for stress tes5ng In 2013, produced 3,000+ addi5onal seedlings for rootstocks

18 Mapping popula+on development for selected species Host Order Species Parent Selected in Hand DNA Collected Paternity Exclusion Complete Established Fagales Northern red oak Quercus rubra Yes 0 Yes Yes 400+ Magnoliales Yellow- poplar Liriodendron tulipifera Yes 550+ Yes No 0 Fabales Honey locust Gleditsia triacanthos Yes 0 Yes No 226+ Sapindales Sugar maple Acer saccharum Yes 1 Yes No 0 Fagales Black walnut Juglans nigra Yes 30 Yes Yes 303 Proteales Sweetgum Liquidambar styraciflua Yes 150+ No NA 0 Lamiales Green ash Fraxinus pennsylvanica Yes 700+ Yes No 0 Cornales Black gum Nyssa sylva@ca Yes 125+ No NA 0

19 Magnoliales: Yellow- poplar (Liriodendron tulipifera) Open pollinated seedlings were grown from an isolated group of three trees in New Franklin, MO to serve as a back up to the UT mapping popula5on, if needed. 1-2 seedlings (~300) are available at MU for plan5ng in spring 2014.

20 Mapping popula+on development for selected species Host Order Species Parent Selected in Hand DNA Collected Paternity Exclusion Complete Established Fagales Northern red oak Quercus rubra Yes 0 Yes Yes 400+ Magnoliales Yellow- poplar Liriodendron tulipifera Yes 550+ Yes No 0 Fabales Honey locust Gleditsia triacanthos Yes 0 Yes No 226+ Sapindales Sugar maple Acer saccharum Yes 150+ No NA 0 Fagales Black walnut Juglans nigra Yes 0 Yes Yes 303 Proteales Sweetgum Liquidambar styraciflua Yes 150+ No NA 0 Lamiales Green ash Fraxinus pennsylvanica Yes 700+ Yes No 0 Cornales Black gum Nyssa sylva@ca Yes 125+ No No 0

21 Sapindales: Sugar maple (Acer saccharum) Angiosperms Monocots Amborellales Magnoliales Laurales Proteales Vitales Eudicots We collected seeds in fall 2012 from a campus tree at MU, resul5ng in 150+ seedlings available for stress tes5ng at Penn State, beginning in spring Core eudiocts Rosids Asterids Malphigiales Fabales Rosales Fagales Myrtales Brassicales Malvales Sapindales Cornales Lamiales

22 Mapping popula+on development for selected species Host Order Species Parent Selected in Hand DNA Collected Paternity Exclusion Complete Established Fagales Northern red oak Quercus rubra Yes 0 Yes Yes 400+ Magnoliales Yellow- poplar Liriodendron tulipifera Yes 550+ Yes No 0 Fabales Honey locust Gleditsia triacanthos Yes 0 Yes No 226+ Sapindales Sugar maple Acer saccharum Yes 150+ Yes No 0 Fagales Black walnut Juglans nigra Yes 0 Yes Yes 303 Proteales Sweetgum Liquidambar styraciflua Yes 150+ No NA 0 Lamiales Green ash Fraxinus pennsylvanica Yes 700+ Yes No 0 Cornales Black gum Nyssa sylva@ca Yes 125+ No NA 0

23 Cornales: Black gum (Nyssa Angiosperms Monocots Amborellales Magnoliales Laurales Proteales Vitales Black gum was included in an earlier version of this project, and efforts to develop a mapping popula5on began in Eudicots Core eudiocts Rosids Malphigiales Fabales Rosales Fagales Myrtales Brassicales Malvales Sapindales Cornales In 2013, we provided ~100 seedlings to PSU for stress tes5ng. Asterids Lamiales