Suitability of Different PCR-DGGE Primer Sets for the Monitoring of Lactic Acid Bacteria in Wine
|
|
- Robyn Dawson
- 6 years ago
- Views:
Transcription
1 Suitbility of Different PCR-DGGE Primer Sets for the Monitoring of Lctic Acid Bcteri in Wine S. Myrhofer*, R. Filipp, D. Lehner, C. Reiterich, W. Kneifel, K.J. Domig BOKU - University of Nturl Resources nd Life Sciences Vienn, Deprtment of Food Science nd Technology, Institute of Food Science, Muthgsse 18, A-1190 Vienn, Austri Submitted for publiction: October 2013 Accepted for publiction: My 2014 Key words: Mlolctic fermenttion, lctic cid bcteri, wine, PCR-DGGE, primer-set Lctic cid bcteri (LAB) ply dul role in winemking s they re the min effectors of mlolctic fermenttion, but some members cn lso cuse wine spoilge. PCR-DGGE hs proved to be quick tool to study the LAB community nd their fluctution in wine. For detecting wine-ssocited LAB by PCR- DGGE, the primer sets WLAB1/WLAB2 GC, WBAC1/WBAC2 GC, Lc1/Lc1o/Lc2 GC, 341f GC /518r nd rpob1/ rpob1o/rpob2 GC were tested nd evluted in this study. The primer systems were ssessed by the seprtion of LAB reference strins on DGGE gels nd by ttributing the resulting mplicons to defined species. Subsequently, the detection of LAB in wine smples nd enrichments thereof ws compred. While the primer systems WBAC1/WBAC2 GC nd 341f GC /518r were not pproprite, the Lc1/Lc1o/Lc2 GC primer set performed well. However, multiple bnds complicted the evlution. The rpob1/rpob1o/rpob2 GC set seemed to be promising for the detection of LAB in wine, lthough further improvements in terms of the detection limit need to be done. Due to the pronounced sensitivity nd the sufficient discrimintion of LAB t species level, the WLAB1/WLAB2 GC primer system ws found to be most suitble for studying the occurrence of LAB in wine. INTRODUCTION Winemking is complex microbil process in which primrily yests, but lso lctic cid bcteri (LAB), ply pivotl roles (Lonvud-Funel, 1999). Mlolctic fermenttion (MLF) cn occur t the end of the lcoholic fermenttion conducted by yests (Lonvud-Funel, 1999). This fermenttion is usully desirble in most of the red wines, some white cultivrs, including Chrdonny, some sprkling wines nd lso in cool-climte Riesling wines (Lerm et l., 2010; Knoll et l., 2012). MLF is the bcteril conversion of L-mlic cid to L-lctic cid nd CO 2 (Bousbours & Kunkee, 1971) nd exerts significnt influences on wine cidity, flvour nd microbiologicl stbility (Moreno-Arribs & Polo, 2005). The min effectors of MLF re LAB. Of these, Oenococcus oeni is the species minly responsible for MLF (Dvis et l., 1986), s it is the species tht hs ccommodted the best to the difficult fermenttion conditions, such s low ph vlues nd high ethnol concentrtions (Wibowo et l., 1988). Due to its prticulr role, this species is commonly used s strter culture to promote MLF (Mills et l., 2005). Furthermore, it is not very frequently reported to be ssocited with off flvours like voltile cidity nd mousiness, spoilge like ropiness, or the formtion of undesirble metbolites such s ethyl crbmte nd biogenic mines, which cn be cused by other wine-relted LAB (Mills et l., 2005). Owing to these observtions there is need to control MLF to enhnce the positive ttributes or to reduce potentil negtive impcts on the prticulr wine (Mills et l., 2005). Trditionl culture-bsed techniques re often used to detect LAB in wine smples (Cho et l., 2011) but, especilly in cse of the min effector O. oeni, up to 14 dys re required to yield results. Such long cultivtion periods, however, do not llow the crrying out of possible oenologicl prevention or opertion in wine production (Pinzni et l., 2004). Thus, severl culture-independent methods (e.g. PCR-DGGE, qpcr) hve been developed becuse they overcome the problems described bove. PCR-denturing grdient gel electrophoresis (DGGE) is commonly used culture-independent fingerprinting technique for the rpid nlysis of microbil communities nd hs been used to nlyse LAB in food (Cocolin et l., 2001). This technique is pplied to seprte mixture of PCR mplicons of the sme size but of different sequences (Ercolini, 2004). Double-strnded PCR mplicons in the gel re subjected to n incresingly denturing environment. The migrtion is stopped when the DNA frgments re completely dentured (Renouf et l., 2007), yielding ptterns tht visulise the genetic diversity of the investigted microbil community (Ercolini, 2004). Implementing new method in the own lbortory lwys requires intensive literture reserch. In terms of PCR-DGGE pplied to study wine LAB microbiot, severl *Corresponding uthor: sigrid.myrhofer@boku.c.t Other uthors: konrd.domig@boku.c.t; wolfgng.kneifel@boku.c.t Acknowledgements: This study ws funded by the Austrin Economic Chmbers (WKO; Wirtschftskmmerpreis 2009) 185
2 PCR-DGGE for LAB Detection 186 different primer sets nd PCR conditions hve lredy been introduced by reserchers. Of these, five primer sets were shortlisted, s they seemed to be pproprite (Lopez et l., 2003; Rntsiou et l., 2004; Endo & Okd, 2005; Be et l., 2006; Renouf et l., 2006; Spno et l., 2007). The objective of this study ws to prtly modify nd extensively test nd evlute these primer systems regrding their suitbility to monitor LAB in wine. The results of this study cn be consulted to investigte the presence of LAB in wine by PCR-DGGE. MATERIALS AND METHODS Strins nd growth conditions Bcteril reference strins nd their corresponding growth conditions (medium nd temperture) used in this study re listed in Tble 1. The strins were grown nerobiclly (85% N 2, 10% CO 2, 5% H 2 ) using MACS VA 500 microerophilic worksttion (Don Whitley Scientific, Shipley, U.K.). In ddition, further LAB (Enterococcus feclis LMG 7937 T, Enterococcus fecium LMG T ; Streptococcus thermophilus LMG 6897 T nd Tetrgenococcus muriticus LMG T ) were included in the tests in order to evlute the specificity of primer Lc1o. Except for Tetrgenococcus muriticus, ll strins were grown on MRS medium (demn, Rogos, Shrpe; Merck, Drmstdt, Germny): Streptococcus thermophilus nerobiclly t 37 C nd Enterococcus feclis s well s Enterococcus fecium TABLE 1 LAB reference strins nd growth conditions. Genus Species Subspecies Source Growth conditions Lctobcillus brevis - LMG 6906 T MRS, 30 C Lctobcillus buchneri - LMG 6892 T MRS, 37 C Lctobcillus csei - LMG 6904 T MRS, 30 C Lctobcillus collinoides - LMG 9194 T MRS, 30 C Lctobcillus coryniformis torquens LMG 9197 T MRS, 30 C Lctobcillus curvtus curvtus LMG 9198 T MRS, 30 C Lctobcillus delbrueckii delbrueckii LMG 6412 T MRS, 37 C Lctobcillus frciminis - LMG 9200 T MRS, 30 C Lctobcillus fermentum - LMG 6902 T MRS, 37 C Lctobcillus fructivorns - LMG 9201 T MRS, 30 C Lctobcillus hilgrdii - LMG 6895 T MRS, 30 C Lctobcillus lindneri - LMG T MRS, 30 C Lctobcillus mli - LMG 6899 T MRS, 30 C Lctobcillus ngeli - LMG T MRS, 37 C Lctobcillus prcsei prcsei LMG T MRS, 30 C Lctobcillus pentosus - LMG T MRS, 30 C Lctobcillus plntrum - LMG 6907 T MRS, 30 C Lctobcillus rhmnosus - LMG 6400 T MRS, 37 C Lctobcillus zee - LMG T MRS, 37 C Lctococcus lctis lctis LMG 6890 T MRS, 30 C Leuconostoc mesenteroides mesenteroides LMG 6893 T MRS, 30 C Oenococcus oeni - LMG 9851 T MLO, 30 C Pediococcus cidilctici - LMG T MRS, 30 C Pediococcus dmnosus - LMG T MRS, 30 C Pediococcus inopintus - LMG T MRS, 30 C Pediococcus prvulus - LMG T MRS, 30 C Pediococcus pentosceus - LMG T MRS, 30 C Weissell confus - LMG 9497 T MRS, 30 C Weissell prmesenteroides - LMG 9852 T MRS, 30 C LMG: BCCM/LMG Bcteri Collection, Ghent University, Ghent, Belgium
3 187 PCR-DGGE for LAB Detection erobiclly t 37 C. Tetrgenococcus muriticus ws cultivted on GYP sodium cette minerl slts medium with 5% sodium chloride (BCCM/LMG Bcteri Collection, Ghent University, Ghent, Belgium; medium 244) under erobic conditions t 30 C. Isoltes of the ccompnying bcteril flor (Bcillus cogulns MSB 29W, Gluconobcter jponicus MSB 32W, Gluconobcter oxydns MSB 107W nd Acetobcter ceti MSB 109W) grown nerobiclly on MLO medium (medium for Leuconostoc oeni; Germn Collection of Microorgnisms nd Cell Cultures (DSMZ); medium 59) t 30 C were lso included. Wine smples nd corresponding microbil enrichment cultures In ddition to the reference strins described bove, wine smples contining n unknown vriety of microorgnisms were investigted. However, s the detection limit of PCR- DGGE is round 10 4 cells/ml or even higher (Andorrà et l., 2008), enrichment cultures of wine smples were lso used for this investigtion. For this purpose, 1 ml of wine smple ws inoculted in 9 ml MRS nd MLO medium nd incubted for seven dys t 30 C under nerobic conditions to enhnce nturlly occurring wine LAB. Initilly, the DNA of 16 wine smples ws isolted for the investigtion. As the first PCR-DGGE results of these smples displyed no or only fint bnds, their enrichments were used minly to compre the performnce of the different primer systems. DNA extrction DNA extrction from 2 ml of pure or enriched cultures ws performed with the Archive Pure DNA Yest & Grm+ Kit (5 Prime, Hmburg, Germny), ccording to the mnufcturer s instructions. The DNA from the wine smple ws extrcted directly using protocol described by Renouf et l. (2009), with minor modifictions. In brief, microbil cells were collected from 50 ml of wine by centrifugtion (4 500 x g, 15 min, 4 C) nd the pellet ws wshed in 600 µl TE buffer (10 mm Tris, 1 mm EDTA). After the next centrifugtion step ( x g, 7 min, 4 C), the superntnt ws discrded nd the pellet resuspended in 300 µl TE buffer. Furthermore, 300 µl of sterile glss beds were dded nd the smples were vortexed for 10 min t 4 C. The superntnt ws mixed with 300 µl cell lysis solution (5 Prime). Subsequently, 200 µl of protein precipittion solution (5 Prime) were dded nd mixed. Precipittion of cellulr frgments ws done on ice for 5 min, followed by centrifugtion step t x g for 3 min t 4 C. The superntnt ws then trnsferred to new 1.5 ml micro-centrifuge tube lredy contining 100 µl of 10% polyvinyl-pyrrolidone solution (PVP, Sigm-Aldrich, St. Louis, Missouri, USA) for the elimintion of tnnins. After vortexing nd further centrifugtion ( x g, 10 min, 4 C), the superntnt ws once more trnsferred to new micro-centrifuge tube contining 300 µl of isopropnol. The tube ws gently mixed by inversion nd centrifuged t x g for 3 min t 4 C. Subsequently, the superntnt ws discrded, nd 300 µl of 70% ethnol were dded to the pellet nd mixed by inversion. A finl centrifugtion step ( x g, 3 min, 4 C) followed, nd the ethnol ws removed crefully. The tube ws dried for 15 min. To rehydrte the DNA, 25 µl TE buffer nd 0.5 µl RNse (4 mg/ml) were dded to the smple overnight t 4 C. The DNA of the wine smples ws stored t -20 C until use. DNA mplifiction nd primers The primer systems WLAB1/WLAB2 GC (Lopez et l., 2003), WBAC1/WBAC2 GC (Lopez et l., 2003), Lc1/Lc2 GC /Lc3 (Wlter et l., 2001; Endo & Okd; 2005), Lc1/Lc1o/Lc2 GC (Wlter et l., 2001; this study) nd 341f GC /518r (Be et l., 2006; Muyzer et l., 1993) were used for the mplifiction of frgments of the bcteril 16S ribosoml RNA (rrna) gene (Tble 2). In ddition, the primer system rpob1/rpob1o/rpob2 GC ws pplied for the dupliction of frgments of the RNA polymerse bet subunit, rpob (Renouf et l., 2006b; Spno et l., 2007). PCR mplifiction ws performed t finl volume of 25 µl with thermocycler (Mstercycler, Eppendorf, Hmburg, Germny) contining combintion of the corresponding primers nd templte DNA, s indicted by the uthors (Muyzer et l., 1993; Wlter et l., 2001; Lopez et l., 2003; Spno et l., 2007), nd 2.5 µl 10 x PCRbuffer (Finnzymes, Vnt, Finlnd), 0.5 µl dntp-mix (10 mm), nd 0.5 µl DNA polymerse (2U/µL, Dynzyme II; Finnzymes). The remining volume ws filled up with sterile distilled wter. Amplicons were run on 2% grose gels, stined with ethidium bromide nd photogrphed under UV trnsillumintion. DGGE The Dcode universl muttion detection system TM (Bio- Rd, Hercules, Cliforni, USA) ws used for the sequencespecific seprtion of PCR products. These were run on 8% (w/v) polycrylmide gels in TAE-buffer (40 mm Triscette; 2 mm N 2 -EDTA x H 2 O, ph 8.5) nd denturing grdient s described originlly or modified ccording to Tble 2. The electrophoresis ws performed t 85 V for 16 h in 1 x TAE-buffer t constnt temperture of 60 C. Bnd-mtching nlysis Using the BioNumerics softwre, version 6.6 (Applied Mths, Sint-Mrtens-Ltem, Belgium), wine-ssocited LAB species were identified by performing bnd mtching. Accordingly, bnds were utomticlly ssigned to bnd clsses defined by the progrm. Uncertin bnds were ignored. The ssignments were corrected mnully, leding to n optimistion of 0% (WBAC, Lc, rpob primer set) or 0.5% (WLAB, 341f GC /518r primer set) nd position tolernce of 0.5% (WLAB primer set) or 1% (WBAC, Lc, 341f GC /518r, rpob primer set). Sequence nlysis After stining the DGGE gel, bnds of interest were excised directly from the gels with sclpel, mixed with 100 µl of 1x PCR buffer, nd incubted overnight t 4 C. Two microlitres of this solution were used to re-mplify the PCR product. The PCR products were purified with the PCRExtrct Mini Kit (5 Prime) nd subjected to commercil sequencing (Eurofins
4 PCR-DGGE for LAB Detection 188 MWG Operon, Ebersberg, Germny). Sequence compiltion nd comprison were performed with the BLASTn progrm. RESULTS AND DISCUSSION PCR-DGGE is fst method for bcteril nlysis, enbling the survey of LAB during winemking (Renouf et l., 2006b). Therefore, selection of primer systems proposed in the literture were checked ginst ech other by testing set of reference strins s well s wine smples nd their enrichments. Lopez et l. (2003) hve lredy shown tht number of primers re not suited, s they lso mplify nonbcteril DNA, resulting in msking of bcteril popultions in DGGE profiles. They therefore developed two new primer sets specificlly for the mplifiction of bcteril 16S rrna gene in wine fermenttion smples. One primer set, termed WLAB1/WLAB2 GC, mplifies LAB, while the other one, termed WBAC1/WBAC2 GC, mplifies LAB nd cetic cid bcteri (AAB). The primer set WLAB1/WLAB2 GC trgets the V4 nd V5 regions of the 16S rrna gene nd produces frgment of pproximtely 400 bp (Lopez et l., 2003). Pure reference cultures were exmined using this primer system. It ws found tht severl LAB species exhibited similr electrophoretic mobilities, becuse ll mplicons were only TABLE 2 Primers tested for PCR-DGGE. Primer Sequence (5 3 ) Trget region Reference Modified PCR-DGGE conditions WLAB1 WBAC1 Lc1 341f GC 518r TCCGGATTTATTGGGCG- TAAAGCGA WLAB2 GC CGCCCGCCGC- GCCCCGCGCCCGGCCC- GCCGCCCCCGCCCCTC- GAATTAAACCACAT- GCTCCA GTCGTCAGCTCGTGTC- GTGAGA WBAC2 GC CGCCCGCCGC- GCCCCGCGCCCGGCCC- GCCGCCCCCGCCCCCCC- GGGAACGTATTCACCGCG AGCAGTAGGGAATCTTC- CA Lc2 GC CGCCCGGGGC- GCGCCCCGGGCGGCCC- GGGGGCACCGGGGGAT- TYCACCGCTACACATG Lc3AGCAG- TAGGGAATCTTCGG Lc1oTGCAG- TAGGGAATTTTCCG CGCCCGCCGC- GCGCGGCGGGC- GGGGCGGGGGCAC- GGGGGGCCTACGGGAG- GCAGCAG ATTACCGCGGCTGCTGG 16S rrna gene (V4 V5) 16S rrna gene (V7 V8) 16S rrna gene (V3) 16S rrna gene (V3) Lopez et l., 2003 Lopez et l., 2003 Wlter et l., 2001 Endo & Okd, 2005 this study PCR mplifiction: Lopez et l., 2003 DGGE denturing grdient: 30 to 55% of ure nd formmide PCR mplifiction: Lopez et l., 2003 DGGE denturing grdient: Lopez et l., 2003 PCR mplifiction: Wlter et l., 2001 DGGE denturing grdient: 35 to 55% of ure nd formmide Muyzer et l., 1993 PCR mplifiction: Be et l., 1993, except touchdown: 0,5 C/cycle DGGE denturing grdient: 35 to 60% of ure nd formmide rpob1 rpob1o rpob2 GC ATTGACCACTTGGGTA- ACCGTCG ATCGATCACTTAG- GCAATCGTCG CGCCCGCCGC- GCGCGGCGGGC- GGGGCGGGGGCAC- GGGGGGGCACGATCAC- GGGTCAAACCACC Modified nucleobses (bold nd itlic letters) rpo gene Renouf et l., 2006b; Spno et l., 2007 (bet-subunit) PCR mplifiction: Spno et l., 2007 DGGE denturing grdient: Renouf et l., 2006b
5 189 PCR-DGGE for LAB Detection displyed in smll rnge of the denturnt concentrtion. Vrying the concentrtion of denturnt of the electrophoresis gel did not improve the seprtion of the tested reference strins. Due to severl copies of the trgeted gene, some species even resulted in multiple bnds, complicting the lloction of bnds to certin LAB species. However, ll of the tested LAB could be detected nd differentited, except for Lb. csei nd Lb. prcsei (Fig. 1). Primer set WBAC1/WBAC2 GC trgets the V7 to V8 regions of the 16S rrna gene nd produced n pproximtely 320 bp mplicon with ll tested reference strins. With reference to Lopez et l. (2003), this primer system works prticulrly well to resolve AAB strins on DGGE. Anyhow, ccording to our results, this primer pir ws not cpble for nlysing the LAB diversity in wine, s the seprtion of the tested reference strins ws not sufficient nd mny species migrted to the sme position (Fig. 1). Nevertheless, it ws possible to discriminte between Lb. csei nd Lb. prcsei. Compred to the WLAB primer system, more multiple nd stronger bnds were obtined with the reference strins, except for Lb. buchneri nd Lb. fructiovorns, which resulted in wek bnds. Along with ll the LAB reference strins, the Bcillus sp. isolte ws lso detected. This isolte, s well s the AAB, produced mplicons t the sme gel positions s LAB. The primer pir Lc1/Lc2 GC ws designed for nlysing the diversity of fecl or vginl LAB nd is specific for the gener Lctobcillus, Pediococcus, Weissell nd Leuconostoc. The primer pir forms 340 bp frgment of the V3 region of the 16S rrna gene (Wlter et l., 2001). An dditionl primer ws constructed by Endo nd Okd (2005) to extend the rnge of detectble LAB for the investigtion of fermented foods. This Lc3 primer ttches t the sme position s Lc1 nd mplifies the 16S rrna gene of Lctococcus, Streptococcus, Enterococcus, Vgococcus nd Tetrgenococcus Testing different primer combintions, Endo nd Okd (2005) observed tht the use of ll three primers in PCR t the sme time ws useful to nlyse LAB diversity. Applying the primer mixture Lc1/Lc2 GC /Lc3, the most relevnt wine LAB, O. oeni, ws not mplified (dt not shown). To overcome this problem, the primer Lc1 or Lc3 ws modified in this work (Lc1o, Tble 2). The specificity of the new primer set Lc1/Lc1o/Lc2 GC ws nlysed using BLASTn nd evluted by performing PCR-DGGE with reference strins nd isoltes of the unwnted, ccompnying bcteril wine micro-flor (for detils see Mterils nd Methods). DGGE bnds were obtined for ll strins of the gener Lctobcillus, Pediococcus, Leuconostoc, Weissell nd O. oeni. In contrst, no bnds were chieved for the gener Enterococcus, Streptococcus, Tetrgenococcus (dt not shown) nd Lctococcus, for which the Lc3 primer ws designed by Endo nd Okd (2005). However, these gener generlly re not relevnt for the fermenttion processes of wines. Due to the genertion of multiple bnds for mny reference strins, the evlution of the results ws difficult. Except for Lb. prcsei, the identifiction of species of the Lctobcillus csei nd Lb. plntrum group, s well s the Pediococcus genus, ws often only possible t genus or species group level (Fig. 1), lthough good seprtion ws chieved for ll other reference strins. No bnds on DGGE gel were obtined for the non-lab bcteri Bcillus cogulns, Acetobcter ceti nd Gluconobcter (Fig. 1). The universl primer set 341f GC /518r, designed by Muyzer et l. (1993), ws pplied successfully by Be et l. (2006) to detect LAB ssocited with wine grpes. It mplifies frgment of the V3 region of the 16S rrna gene, forming 233 bp product. The PCR product from the Lb. fructivorns reference strin ws wek when using this primer set without GC-clmp, nd resulted in no visible bnd on the DGGE gel. However, the distribution of the bnds of ll other LAB reference strins ws good, lthough multiple bnds per strin ppered (Fig. 1). In ddition, different LAB of one genus or species group (e.g. the Pd. dmnosus, Pd. prvulus, Pd. inopintus, Lctobcillus csei nd Lb. plntrum group) showed identicl results on the DGGE gel. As this primer set is universl, fint bnds of AAB nd the Bcillus isolte were displyed, but not in the concentrtion rnge of LAB. As ribosoml genes re present in severl copies with different sequences (Rntsiou et l., 2004), ll primer systems considered generted diverse mplicons, resulting in multiple bnds on the gel. Thus, nother primer set trgeting the RNA polymerse bet subunit gene rpob, which is only present s single copy (Rntsiou et l., 2004), ws lso included in the tests. This primer set, originlly developed by Renouf et l. (2006), hd lredy been used to study the effect of different oenologicl prctices on LAB popultions nd their evolution during winemking. Bsed on the rpob1/rpob1o/rpob2 GC primer system, the bnds of the reference strins were well seprted within this study (Fig. 2). However, the optimlly expected single bnd per strin ppered s min bnd with wek double bnds in its neighbourhood (Renouf et l., 2006). Amongst others, these bnds my be due to n enzymtic process involving the TAQ polymerse (Jnse et l., 2004). Nevertheless, the min bnds were clerly seprted nd visible. In the cse of wine smples, the unmbiguous detection of min bnds could even be improved with mixture of vrious species. Otherwise, reference strins of some LAB species lredy found in wine could not be detected beside the ccompnying bcteril flor (e.g. Lc. lctis, Lb. brevis, Lb. csei, Lb. coryniformis, Lb. curvtus, Lb. delbrueckii, Lb. fermentum, Lb. fructivorns, Lb. hilgrdii, Lb. lindneri, Lb. ngeli, Lb. zee nd Pd. inopintus), or generted only fint bnds (e.g. Lb. prcsei nd Pd. prvulus). Although Renouf et l. (2006) could determine the wine-relevnt species Lb. brevis nd Lb. hilgrdii by PCR-DGGE, we could not produce mplicons for the used reference strins of these two species in our study when pplying this primer system. However, Lb. brevis could be identified in one of the subsequently tested smples. As PCR-DGGE ptterns obtined with the reference strins should llow the tenttive identifiction of DNA frgments in ech smple, wine-ssocited LAB species were identified by mtching their bnd distnces to those of the reference strins using the BioNumerics softwre. O. oeni, Lb. brevis, members of the Pediococcus genus, the Lb. buchneri, Lb. csei nd Lb. plntrum group were
6 PCR-DGGE for LAB Detection 190 FIGURE 1 Digitised PCR-DGGE ptterns of ctive rnges from 29 reference strins, four ccompnying flor isoltes, one wine smple nd 26 wine enrichments with primer sets WLAB1/WLAB2 GC (30% to 80%), WBAC1/WBAC2 GC (45% to 75%), Lc1/Lc1o/ Lc2 GC (25% to 85%) nd 341f GC /518r (10% to 80%). Verticl lines indicte the specified bnd clsses. The bnds lbelled 1 to 56 re described in Tble 4. bnd clss ssignment
7 191 PCR-DGGE for LAB Detection TABLE 3 Detection of LAB in wine smples nd/or their enrichment cultures bsed on bnd position, bnd pttern of species, sequencing results nd comprison between different primer systems. Smple Primer set WLAB1/WLAB2 GC WBAC1/WBAC2 GC Lc1/Lc1o/Lc2 GC 341f GC /518r rpob1/rpob1o/rpob2 GC 1 b O. oeni, Pd. dmnosus Pd. prvulus O. oeni, Pediococcus Pediococcus O. oeni, Pd. dmnosus 2 c O. oeni O. oeni O. oeni O. oeni, Pediococcus O. oeni 3 c O. oeni, Pd. dmnosus O. oeni, Pd. dmnosus O. oeni, Pediococcus Pediococcus O. oeni, Pd. dmnosus 4 c Pd. dmnosus Pd. dmnosus Pediococcus Pediococcus Pd. dmnosus 5 c O. oeni O. oeni O. oeni O. oeni, Pediococcus O. oeni 6 c Le. mesenteroides, Pd. dmnosus 7 c Le. mesenteroides, Pd. prvulus, Lb. csei-group Pd. dmnosus O. oeni, Pediococcus Pediococcus O. oeni, Pd. dmnosus Lb. prcsei O. oeni, Pd. prvulus, Lb. prcsei Pediococcus, Lb. cseigroup O. oeni, Pd. prvulus 8 c Le. mesenteroides, Pd. prvulus Pd. prvulus Pediococcus Pediococcus Pd. prvulus 9 c Lb. buchneri/hilgrdii Lb. prcsei Lb. buchneri, Lb. prcsei Lb. buchneri, Lb. csei-group O. oeni, Lb. buchneri, Lb. rhmnosus 10 c Lb. csei-group, Pd. prvulus Lb. prcsei Lb. prcsei, Pediococcus Lb. csei-group, Pediococcus O. oeni, Pd. prvulus 11 c Lb. csei-group Lb. prcsei Lb. prcsei Lb. csei-group Lb. rhmnosus 12 c Lb. csei-group, Pd. prvulus Lb. prcsei Lb. prcsei, Pediococcus Lb. csei-group, Pediococcus Lb. buchneri, Lb. rhmnosus 13 c Lb. csei-group Lb. prcsei Lb. prcsei Lb. csei-group Lb. rhmnosus 14 c Lb. buchneri Lb. buchneri Lb. buchneri Lb.buchneri Lb. buchneri 15 c O. oeni, Pd. prvulus O. oeni O. oeni, Pediococcus Pediococcus O. oeni, Pd. prvulus 16 c Pd. prvulus Pd. prvulus O. oeni, Pediococcus Pediococcus O. oeni, Pd. prvulus 17 c Pd. prvulus Pd. prvulus O. oeni, Pediococcus Pediococcus Pd. prvulus, Pd. dmnosus 18 c O. oeni O. oeni O. oeni O. oeni, Geminicoccus O. oeni 19 c Lb. brevis Lb. brevis Lb. brevis Lb. brevis, Enterobcter Lb. brevis 20 c Pd. prvulus Pd. prvulus, Gluconobcter 21 c Lb. buchneri/hilgrdii Acetobcter, Gluconobcter Pd. prvulus Pediococcus Pd. prvulus Lb. diolivorns Lb. diolivorns/hilgrdii Lb. buchneri
8 PCR-DGGE for LAB Detection 192 TABLE 3 (CONTINUED) Primer set Smple WLAB1/WLAB2 GC WBAC1/WBAC2 GC Lc1/Lc1o/Lc2 GC 341f GC /518r rpob1/rpob1o/rpob2 GC 22 c Lb. csei-group Lb. prcsei Lb. prcsei Lb. csei-group Lb. prcsei 23 c O. oeni O. oeni, Gluconobcter O. oeni O. oeni - Lb. prcsei Lb. diolivorns/hilgrdii, Lb. csei-group 24 c Lb. buchneri/hilgrdii Lb. prcsei Lb. diolivorns/hilgrdii, Lb. prcsei 25 c Lb. plntrum Lb. plntrum-group Lb. plntrum-group Lb. plntrum-group Lb. plntrum 26 c Lb. plntrum Lb. plntrum-group Lb. plntrum-group Lb. plntrum-group Lb. plntrum 27 c Pd. prvulus Pd. prvulus Pd. prvulus Pediococcus Pd. prvulus The highest bnd ppered in ll strin mixtures (used s mrker, dt not shown) nd smples with strong bnd signls. It therefore ws not used for differentition. b wine smple, c enrichment smple FIGURE 2 Digitised PCR-DGGE ptterns of the ctive rnge (10% to 75%) from 29 reference strins, four ccompnying flor isoltes, one wine smple nd 26 wine enrichments with primer set rpob1/rpob1o/rpob2 GC. Verticl lines indicte the specified bnd clsses. The bnds lbelled 57 to 67 re described in Tble 4. bnd clss ssignment
9 193 PCR-DGGE for LAB Detection TABLE 4 Similrities of sequenced bnds. Primer system Bnd Closest reltives GenBnk ccession no. % sequence similrity WLAB 1, 10 O. oeni NR_ % 2, 3 Pd. dmnosus NR_ % 4, 9 Lb. csei, Lb. prcsei, Lb. zee NR_ , NR_ , NR_ % 5-8 Lb. buchneri NR_ % 11 Lb. plntrum, Lb. prplntrum, Lb. pentosus NR_ , NR_ , NR_ % 12 Pd. prvulus NR_ % WBAC Pd. dmnosus NR_ % 16, 18 Pd. prvulus, Pd. ethnolidurns NR_ , NR_ % 17 Lb. prbuchneri, Lb. buchneri NR_ , NR_ % 19 Lb. brevis NR_ % 20, 24 Gluconobcter NR_ , NR_ , NR_ , NR_ % 21 Acetobcter NR_ , NR_ , NR_ % 22 Lb. prcsei, Lb. csei NR_ , NR_ % 23 O. oeni NR_ % Lc 25 Pd. dmnosus NR_ % 26, 27, 39, 42 Pd. prvulus NR_ % 28, 29, Lb. buchneri NR_ % Lb. prcsei, Lb. csei, Lb. zee NR_ , NR_ , NR_ % 38 Lb. brevis NR_ % 40 Lb. diolivorns NR_ % 41 Lb. plntrum, Lb. prplntrum, Lb. pentosus NR_ , NR_ , NR_ % 341f Pd. prvulus, Pd. dmnosus, Pd. inopintus NR_ , NR_ , NR_ % Lb. buchneri NR_ % 49, 55 Lb. rhmnosus, Lb. csei, Lb. prcsei, Lb. zee NR_ , NR_ , NR_ , NR_ %, 99% 50 O. oeni NR_ % 51 Geminicoccus sp. NR_ % 52 Lb. brevis NR_ % 53 Klebsiell sp., Erwini sp., Enterobcter sp. NR_ , NR_ , NR_ % 54 Lb. diolivorns, Lb. hilgrdii NR_ , NR_ % 56 Lb. plntrum, Lb. prplntrum, Lb. pentosus NR_ , NR_ , NR_ % rpob 57, 58 Pd. dmnosus DQ %, 100% 59, 60, 64 O. oeni CP % Pd. prvulus AY %-100% 65 Lb. brevis AP % 66 Lb. prcsei, Lb. csei CP , HE % 67 Lb. plntrum CP % sme similrity for ll sequenced bnds
10 PCR-DGGE for LAB Detection 194 detectble when investigting the smples using ech selected primer system (Tble 3). In ddition to these LAB, wek bnds corresponding to the species Le. mesenteroides were displyed by the WLAB set. This species, s well s the species detected by ll primer sets, belong to the min LAB isolted from must nd wines (Pozo-Byón et l., 2009). Compred to the other primer systems used in our experiment, O. oeni ws rrely detected by the 341f GC /518r primer set, wheres this species ws frequently identified by the Lc nd rpob primer systems with specific primers for O. oeni. Furthermore, species of the Lb. buchneri group nd the Pediococcus genus were rrely found by the WBAC primer set. The detection of the diversity of species by vrious primer sets my be influenced by their differing ffinity to different species (Be et l., 2006). According to our observtions, the primer pir used itself lso ffects the detection limit of PCR-DGGE. Thus, O. oeni could not or hrdly be identified in smple 23 by the rpob nd 341f GC /518r primer sets respectively, wheres its presence ws clerly detected by ll other primer systems (Tble 3). This detection limit even increses when competitive templte DNAs re present (Andorrà et l., 2008). Furthermore, Be et l. (2006), s well s Renouf et l. (2006b), concluded independently tht their pplied primer sets were only ble to revel the predominnt species. When pplying the primer sets described bove to investigte 27 wine smples nd enrichment cultures, the sme species were mostly detected by the Lc1/Lc1o/Lc2 GC nd WLAB1/WLAB2 GC primer sets, followed by the rpob1/rpob1o/rpob2 GC primer systems. The biggest diversity of LAB species ws lso verified by these primer sets. The WBAC1/WBAC2 GC nd 341f GC /518r primer systems showed the poorest complince. Bsed on the sequence nlysis performed for selected bnds (Tble 4), it turned out tht the similrity of the sequences of LAB bnds generted by primers trgeting the 16S rrna gene (WLAB, WBAC, Lc nd 341f GC /518r systems) with those vilble in the dtbse ws 97%, wheres ll tested rpob sequences corresponded to dtbse sequences with similrity of 98%. Furthermore, sequences generted by the rpob primer set were more discrimintive for the identifiction of relted LAB species thn those produced by 16S rrna gene primer systems, which is in ccordnce with the literture (Renouf et l., 2006; Lv et l., 2012). CONCLUSIONS Due to poor complince with the other primer sets, the WBAC1/WBAC2 GC nd 341f GC /518r primer systems re not suitble to investigte the diversity of LAB involved in winemking. In ddition, multiple bnds were frequently produced for the reference strins tested, complicting the lloction of prticulr bnd to defined species. Compred to these primer systems, the Lc set with the modified primer Lc1o exhibited slightly better performnce, lthough multiple bnds lso were obtined. Due to the proper seprtion of different species on the gel, s well s their verifiction by distinctive sequence nlysis, the rpob1/rpob1o/rpob2 GC primer system seems to be promising tool for monitoring the evolution of wine LAB. However, improvements should be mde, s the detection limit of this set seems to be higher thn tht of the other primer sets. Owing to its pronounced sensitivity nd its cpbility of discriminting to species level, the WLAB1/WLAB2 GC primer set turned out to be dvntgeous for LAB detection purposes in wine. LITERATURE CITED Andorrà, I., Lndi, S., Ms, A., Guillmón, J.M. & Esteve-Zrzoso, B., Effect of oenologicl prctices on microbil popultions using culture-independent techniques. Food Microbiol. 25, Be, S., Fleet, G.H. & Herd, G.M., Lctic cid bcteri ssocited with wine grpes from severl Austrlin vineyrds. J. Appl. Microbiol. 100, Bousbours, G.E. & Kunkee, R.E., Effect of ph on mlo-lctic fermenttion in wine. Am. J. Enol. Vitic. 22, Cho, G.-S., Kruß, S., Huch, M., Du Toit, M. & Frnz C.M.A.P., Development of quntittive PCR for detection of Lctobcillus plntrum strters during wine mlolctic fermenttion. J. Microbiol. Biotechnol. 21, Cocolin, L., Mnzno, M., Cntoni, C. & Comi, G., Denturing grdient gel electrophoresis nlysis of the 16S rrna gene V1 region to monitor dynmic chnges in the bcteril popultion during fermenttion of Itlin susges. Appl. Environ. Microbiol. 67, Dvis, C.R., Wibowo, T.H.L. & Fleet, G.H., Growth nd metbolism of lctic cid bcteri during nd fter mlolctic fermenttion of wines t different ph. Appl. Environ. Microbiol. 51, Endo, A. & Okd, S., Monitoring the lctic cid bcteri diversity during shochu fermenttion by PCR-denturing grdient gel electrophoresis. J. Biosci. Bioeng. 99, Ercolini, D., PCR-DGGE fingerprinting: Novel strtegies for detection of microbes in food. J. Microbiol. Methods. 56, Jnse, I., Bok, J. & Zwrt, G., A simple remedy ginst rtifctul double bnds in denturing grdient gel electrophoresis. J. Microbiol. Methods. 57, Knoll, C., Fritsch, S., Schnell, S., Grossmnn, M., Krieger-Weber, S., Du Toit, M. & Ruhut, D., Impct of different mlolctic fermenttion inocultion scenrios on Riesling wine rom. World J. Microbiol. Biotechnol. 28, Lerm, E., Engelbrecht L. & Du Toit, M., Mlolctic fermenttion: The ABC s of MLF. S. Afr. J. Enol. Vitic. 31, Lonvud-Funel, A., Lctic cid bcteri in the qulity improvement nd deprecition of wine. Antonie vn Leeuwenhoek 76, Lopez, I., Ruiz-Lrre, F., Cocolin, L., Orr, E., Phister, T., Mrshll, M., Vndergheynst, J. & Mills, D.A., Design nd evlution of PCR primers for nlysis of bcteril popultions in wine by denturing grdient gel electrophoresis. Appl. Environ. Microbiol. 69, Lv, X.-C., Weng, X., Zhng, W., Ro, P.F. & Ni, L., Microbil diversity of trditionl fermenttion strters for Hong Qu glutinous rice wine s determined by PCR-medited DGGE. Food Control 28, Mills, D.A., Rwsthorne, H., Prker, C., Tmir, D. & Mkrov, K., Genomic nlysis of Oenococcus oeni PSU-1 nd its relevnce to winemking. FEMS Microbiol. Rev. 29,
11 195 PCR-DGGE for LAB Detection Moreno-Arribs, M.V. & Polo, M.C., Winemking microbiology nd biochemistry, current knowledge nd future trends. Crit. Rev. Food Sci. nd Nutr. 45, Muyzer, G., De Wl, E.C. & Uitterlinden, A.G., Profiling of complex microbil popultions by denturing grdient gel electrophoresis nlysis of polymerse chin rection mplified genes coding for 16S rrna. Appl. Environ. Microbiol. 59, Pinzni, P., Boncini, L., Pzzgli, M., Orlndo, C., Guerrini, S. & Grnchi, L., Rpid detection of Oenococcus oeni in wine by rel-time quntittive PCR. Let. Appl. Microbiol. 38, Pozo-Byón, M.A., Prdo, I., Ferrer, S. & Moreno-Arribs, M.V., Moleculr pproches for the identifiction nd chrcteristion of oenologicl lctic cid bcteri. Afr. J. Biotechnol. 8, Rntsiou, K., Comi, G. & Cocolin, L., The rpob gene s trget for PCR-DGGE nlysis to follow lctic cid bcteril popultion dynmics during food fermenttions. Food Microbiol. 21, Renouf, V., Clisse, O. & Lonvud-Funel, A., RpoB gene: A trget for identifiction of LAB cocci by PCR-DGGE nd melting curves nlysis in rel time PCR. J. Microbiol. Methods 67, Renouf, V., Clisse, O. & Lonvud-Funel, A., Inventory nd monitoring of wine microbil consorti. Appl. Microbiol. Biotechnol. 75, Renouf, V., Clisse, O., Miot-Sertier, C. & Lonvud-Funel, A., 2006b. Lctic cid bcteri evolution during winemking: Use of rpob gene s trget for PCR-DGGE nlysis. Food Microbiol. 23, Renouf, V., Vyssieres, L.C., Clisse, O. & Lonvud-Funel, A., Genetic nd phenotypic evidence for two groups of Oenococcus oeni strins nd their prevlence during winemking. Appl. Microbiol. Biotechnol. 83, Spno, G., Lonvud-Funel, A., Clisse, O. & Mss, S., In vivo PCR-DGGE nlysis of Lctobcillus plntrum nd Oenococcus oeni popultions in red wine. Curr. Microbiol. 54, Wlter, J., Hertel, C., Tnnock, G.W., Lis, C.M., Munro, K. & Hmmes, W.P., Detection of Lctobcillus, Pediococcus, Leuconostoc, nd Weissell species in humn feces by using group-specific PCR primers nd denturing grdient gel electrophoresis. Appl. Environ. Microbiol. 67, Wibowo, D., Fleet, G.H., Lee, T.H. & Eschenbruch, R.E., Fctors ffecting the induction of mlolctic fermenttion in red wines with Leuconostoc oenos. J. Appl. Bcteriol. 64,
Chemical Characterization of Wines Fermented
APPLIED MICROBIOLOGY, JUIY, 1966 Copyright 1966 Americn Society for Microbiology Vol. 14, No. 4 Printed in U.S.A. Chemicl Chrcteriztion of Wines Fermented with Vrious Mlo-lctic Bcteri' GORDON J. PILONE,2
More informationEvaluation of Yogurt with Enhanced Cysteine Content
Diry Foods Evlution of Yogurt with Enhnced Cysteine Content S. Bl nd K. A. Schmidt Summry Amino cids re the uilding locks of protein nd ssist with metolism in the ody. In the humn ody, the mino cid cysteine
More informationClonal Selection of the Greek Grape Wine Cultivar Xinomavro
Clonl Selection of the Greek Grpe Wine Cultivr Xinomvro H.C. Spinthiropoulou nd.. Leventkis itro Hells S.A Niseli, Alexndri A.G. Goulioti nd.. Mrinos Ampeloeniki Ltd Technologicl Prk of Thessloniki.. Stvrkkis
More informationInfluence of Fermentation Temperature on Composition and Sensory Properties of Semillon and Shiraz Wines
Influence of Fermenttion on Properties of Semillon nd Shirz Wines 235 Influence of Fermenttion Temperture on Composition nd Sensory Properties of Semillon nd Shirz Wines Andrew Reynolds, 1* Mrgret Cliff,
More informationScienceDirect. Development of a method for manufacturing noodles from finger millet
Avilble online t www.sciencedirect.com ScienceDirect Procedi Food Science 6 (2016 ) 293 297 Interntionl Conference of Sbrgmuw University of Sri Lnk 2015 (ICSUSL 2015) Development of method for mnufcturing
More informationPostharvest Application of 1-Methylcyclopropene (1-MCP) Extends Shelf Life of Kiwifruit
Posthrvest Appliction of 1-Methylcyclopropene (1-MCP) Extends Shelf Life of Kiwifruit C.M. Cntin 1, D. Holcroft 2 nd C.H. Crisosto 1 1 University of Cliforni, Dvis, Deprtment of Plnt Sciences, Dvis, CA
More information2011 ORGANIC SOYBEAN VARIETY TRIAL MATERIALS AND METHODS
2011 ORGANIC SOYBEAN VARIETY TRIAL In 2011, the University of Vermont Extension conducted soyben vriety tril in Alburgh, VT. The purpose of this tril ws to provide yield comprisons of food- nd feed-grde
More informationCARBOHYDRATE FERMENTATION REACTIONS OF STAPHYLOCOCCI*
CARBOHYDRATE FERMENTATION REACTIONS OF STAPHYLOCOCCI* GEORGE H. CHAPMAN AND MERRITT H. STILES Clinicl Reserch Lbortory, New York, N. Y. A survey of the literture indictes tht wide vritions in technic nd
More informationIndicate (X) client(s) to whom this final report is submitted. Replace any of these with other relevant clients if required. FINAL REPORT FOR 2011/12
Finl report 1 CFPA Cnning Fruit Producers Assoc. Sumit to: Wiehhn Victor Tel: +27 (0)21 872 1501 inmk@mwe.co.z SAAPPA / SASPA / SAT Fruitgro Science Sumit to: Louise Lieenerg Tel: +27 (0)21 882 8470/1
More informationAsian Journal of Food and Agro-Industry ISSN Available online at
As. J. Food Ag-Ind. 2010, 3(04), 368-372 Asin Journl of Food nd Agro-Industry ISSN 1906-3040 Aville online t www.jofi.info Reserch Article Effect of drying nd frying time on texturl nd sensory chrcteristics
More informationSemi-commercial evaluation of SmartFresh with South African export avocados in static containers at the Westfalia packhouse during 2002
South Africn Avocdo Growers' Assocition Yerbook. 2003. 26:55-64 Semi-commercil evlution of SmrtFresh with South Africn export vocdos in sttic continers t the Westfli pckhouse during 2002 D Lemmer #, J
More informationThe Effect of Oat Bran Fiber on Texture, Moisture and Palatability of Pumpkin Muffins
The Effect of Ot Brn Fiber on Texture, Moisture nd Pltbility of Pumpkin Muffins M. Montrsi & K. Reidenbch 11/19/2012 NUTR 453 Finl Report Abstrct: Reserch hd shown n lrmingly low fiber intke of United
More informationTechnical Brief In situ Measurements of Dissolved Oxygen during Low-Level Oxygenation in Red Wines
Technicl Brief In situ Mesurements of Dissolved Oxygen during Low-Level Oxygention in Red Wines V. Felipe Lurie, 1 Robert Lw, 2 Willy S. Joslin, 2 nd Andrew L. Wterhouse 3 * Abstrct: Dissolved oxygen ws
More informationEffect of Ultra-high Pressure Treatment on the Chemical Properties, Colour and Sensory Quality of Young Red Wine
Effect of Ultr-high Pressure Tretment on the Chemicl Properties, Colour nd Sensory Qulity of Young Red Wine X. Sun#, X. Chen#, L. Li, T. M, F. Zho, W. Hung, J. Zhn* College of Food Science nd Nutritionl
More informationFood Research International
Food Reserch Interntionl 43 (2010) 2284 2288 Contents lists vilble t ScienceDirect Food Reserch Interntionl journl homepge: www.elsevier.com/locte/foodres Effect of honey powder on dough rheology nd bred
More informationComparative of fermentation in red and yellow watermelon juice by lactic acid bacteria
Comprtive of fermenttion in red nd yellow wtermelon juice by lctic cid bcteri 1 Zhr sdt lvi Islmic Azd University Deprtment of Food Science nd Technology Vrmin, Irn zhr.lvi110@yhoo.com 2 Seyed HdiRzvi,
More information2014 Sunflower Planting Date Trial
214 Sunflower Plnting Dte Tril Dr. Hether Drby, UVM Extension Agronomist Sr Ziegler, Eric Cummings, Susn Monhn, nd Julin Post UVM Extension Crops nd Soils Technicins (82) 524-651 Visit us on the web t
More informationAcknowledgements. Recent Research: Vine Balance and Fruit Thinning. Brief Outline. Take Aways. How many of you have read? Vine Balance.
Recent Reserch: Vine Blnce nd Fruit Thinning Sustinble Ag Expo Monterey County Jim Wolpert Extension Viticulturist Deprtment of Viticulture nd Enology UC Dvis Acknowledgements Funding Americn Vineyrd Foundtion
More informationRapid Reduction in Aroma Volatiles of Pacific Rose Apples in Controlled Atmospheres
Rpid Reduction in Arom Voltiles of Pcific Rose Apples in Controlled Atmospheres H.J. Tough nd E.W. Hewett Centre for Posthrvest nd Refrigertion Reserch Institute of Nturl Resources, Mssey University Plmerston
More informationMicrobial species associated with different sections of broccoli harvested from three regions in Australia
Interntionl Journl of Food Microbiology 0 (000) 1 www.elsevier.nl/ locte/ ijfoodmicro Microbil species ssocited with different sections of broccoli hrvested from three regions in Austrli b b, * Msdin Pdg,
More informationEVALUATION OF SEED AND IN-FURROW AT-PLANTING APPLIED INSECTICIDES ON SORGHUM
EVALUATION OF SEED AND IN-FURROW AT-PLANTING APPLIED INSECTICIDES ON SORGHUM Texs Agriculturl Experiment Sttion, Corpus Christi, TX, nd Michel nd Wlter Kuck Frm, Lvc County, 1999 Roy D. Prker, Emil D.
More informationResearch Article Oenological and Quality Characteristic on Young White Wines (Sauvignon Blanc): Effects of High Hydrostatic Pressure Processing
Hindwi Journl of Food Qulity Volume 2017, rticle ID 8524073, 12 pges https://doi.org/10.1155/2017/8524073 Reserch rticle Oenologicl nd Qulity Chrcteristic on Young White Wines (Suvignon Blnc): Effects
More informationVALUE ADDITION OF IDLI THROUGH INCORPORATION OF GREENS AND LEGUMES
VALUE ADDITION OF IDLI THROUGH INCORPORATION OF GREENS AND LEGUMES Thesis submitted to Pondicherry University for the wrd of the degree DOCTOR OF PHILOSOPHY in FOOD SCIENCE AND NUTRITION By S. UMA MAHESWARI
More informationKiller Yeasts: Incidence in the Ecology of Spontaneous Fermentation
352 Gutiérrez et l. Killer Yests: Incidence in the Ecology of Spontneous Fermenttion A.R. Gutiérrez, 1 * S. Epifnio, 2 P. Grijo, 2 R. López, 2 nd P. Sntmrí 2 The occurrence of the killer chrcter ws studied
More informationTALLINN, ESTONIA APRIL 25, 2017 TECHNICAL OVERVIEW ON CIDER PRODUCTION
TALLINN, ESTONIA APRIL 25, 2017 TECHNICAL OVERVIEW ON CIDER PRODUCTION 23 LALLEMAND OENOLOGY TALLINN, ESTONIA, APRIL 25, 2017 TECHNICAL OVERVIEW ON CIDER PRODUCTION PROCEEDINGS OF THE XXVII es ENTRETIENS
More informationINTERNATIONAL JOURNAL OF FOOD ENGINEERING
INTERNATIONAL JOURNAL OF FOOD ENGINEERING Discrimintion of Wines Produced from Cbernet Suvignon Grpes Treted with Aqueous Ethnol Post-Bloom Using n Electronic Nose Amnd Mrtin 1, Kumr Mllikrjun, 2 nd Bruce
More informationElectronic Nose Evaluation of the Effects of Canopy Side on Cabernet franc (Vitis vinifera L.) Grape and Wine Volatiles
Electronic Nose Evlution of the Effects of Cnopy Side on Cbernet frnc (Vitis vinifer L.) Grpe nd Wine Voltiles Ymun S. Devrjn, 1 Bruce W. Zoecklein, 2 * Kumr Mllikrjunn, 3 nd Denise M. Grdner 1 Abstrct:
More informationHow to get the best eating quality of pork
The effect of ft on the eting qulity of pork Ensuring the best eting qulity Ensuring the best flvours The effect of cooking ft on the eting qulity of pork How to get the best eting qulity of pork The cooking
More informationDNA extraction method as per QIAamp DNA mini kit (Qiagen, Germany)
APPENDIX 3 (MOLECULAR TECHNIQUES) 3.2.2a) DNA extraction method as per QIAamp DNA mini kit (Qiagen, Germany) Two hundred microliters (200 µl) of the EDTA blood was added to 200 µl of Buffer AL and 20 µl
More informationSeparation of Sunlight and Temperature Effects on the Composition of Vitis vinifera cv. Merlot Berries
Sunlight nd Temperture Effects on Merlot Berries 171 Seprtion of Sunlight nd Temperture Effects on the Composition of Vitis vinifer cv. Merlot Berries S.E. Spyd, 1 * J.M. Trr, 2 D.L. Mee, 1 nd J.C. Ferguson
More informationThe Effect of Acetic and Lactic Acid on the Oil Uptake, Texture and Color of Rice (Sang Tarom) During Cooking
World Applied Sciences Journl 4 (2): 183-187, 2008 ISSN 1818-4952 IDOSI Publictions, 2008 The Effect of Acetic nd Lctic Acid on the Oil Uptke, Texture nd Color of Rice (Sng Trom) During Cooking 1 1 2 E.
More informationColonization of chasmothecia of grapevine powdery mildew by Ampelomyces quisqualis
Biocontrol of powdery mildew Coloniztion of chsmotheci of grpevine powdery mildew y Ampelomyces quisqulis Drio Angeli, Frnc Vlentini, Chir Msiero, Oscr Giovnnini, Ilri Pertot Fondzione Edmund Mch, S.Michele
More informationPREPARATION AND HANDLING FRESH-CUT ROOT VEGETABLES. MERETE EDELENBOS DEPT. OF FOOD SCIENCE AARHUS UNIVERSITY DENMARK
PREPARATION AND HANDLING FRESH-CUT ROOT VEGETABLES MERETE EDELENBOS DEPT. OF FOOD SCIENCE AARHUS UNIVERSITY DENMARK merete.edelenos@food.u.dk Preprtion nd hndling fresh-cut root vegetles Production Hndling
More informationPerformance of Seyval Blanc Grape in Four Training Systems Over Five Years
CROP PRODUCTON HORTSCENCE 37(7): 1023-1027.2002. Performnce of Seyvl Blnc Grpe in Four Trining Systems Over Five Yers D. Ferree, T. Steiner, J. Gllnder, D. Scurlock, G. Johns, nd R. Riesen Deprtment of
More informationApplication of Toasted Oak and Micro-oxygenation to Ageing of Cabernet Sauvignon Wines
Appliction of Tosted Ok nd Micro-oxygention to Ageing of Cernet Suvignon Wines Dr. Jeff McCord StVin Inc., Cliforni, USA Introduction For mny yers, tosted ok in the form of rrels hs een used for wine storge
More informationJ. //«/. Brew., May-June, 1976, Vol. 82, pp FACTORS RESPONSIBLE FOR THE DECREASE IN ph DURING BEER FERMENTATIONS
J. //«/. Brew., My-June, 19, Vol. 82, pp. 149-3 149 ACTOS ESPONSIBLE O THE DECEASE IN ph DUING BEE EMENTATIONS By N. Coote nd B. H. Kirsop (Brewing Industry eserch oundtion. Nutfield, edhill, Surrey, Englnd)
More informationImpact of Shoot and Cluster Thinning on Yield, Fruit Composition, and Wine Quality of Corot noir
Impct of Shoot nd Cluster Thinning on Yield, Fruit Composition, nd Wine Qulity of Corot noir Qun Sun, 1 Gvin L. Scks, 2 Steven D. Lerch, 3 nd Justine E. Vnden Heuvel 4,5 * Abstrct: Cluster thinning (CL),
More informationFactors Associated with Dough Stickiness as Sensed by Attenuated Total Reflectance Infrared Spectroscopy
Fctors Associted with Dough Stickiness s Sensed by Attenuted Totl Reflectnce Infrred Spectroscopy Ewoud J. J. vn Velzen, 1,2 John P. M. vn Duynhoven, 1 Pul Pudney, 3 Peter L. Weegels, 1 nd John H. vn der
More informationThe Effect of Quinoa on the Taste and Texture of Chocolate Chip Cookies. Hannah Doren Tarryn Hake Mitch Simmonds NUTR 453.
The Effect of Quino on the Tste nd Texture of Chocolte Chip Cookies Hnnh Doren Trryn Hke Mitch Simmonds NUTR 453 November 19, 2012 Title: The Effect of Quino on the Tste nd Texture of Chocolte Chip Cookies
More informationEffects of chemical treatments on dormancy breaking and some sprouting characteristics of two potato cultivars in different tuber sizes
Avilble online t www.pelgireserchlibrry.com Europen Journl of Experimentl Biology, 2014, 4(4):98-102 ISSN: 2248 9215 CODEN (USA): EJEBAU Effects of chemicl tretments on dormncy breking nd some sprouting
More informationTHE EXTRACTION KINETICS OF ANTHOCYANINS AND PROANTHOCYANIDINS FROM GRAPE TO WINE IN THREE DIFFERENT VARIETIES
THE EXTRACTION KINETICS OF ANTHOCYANINS AND PROANTHOCYANIDINS FROM GRAPE TO WINE IN THREE DIFFERENT VARIETIES An B. BAUTISTA-ORTÍN 1, Nir BUSSE-VALVERDE 1, José I. FERNÁNDEZ-FERNÁNDEZ 2, Encrn GÓMEZ-PLAZA
More informationPreview. Chapter 3. Labor Productivity and Comparative Advantage: The Ricardian Model
hpter 3 Lbor roductivity nd omprtive Advntge: The Ricrdin Model review Opportunity costs nd comprtive dvntge roduction possibilities Reltive supply, reltive demnd & reltive prices Trde possibilities nd
More informationCharacterization of Fruitbody Morphology on Various Environmental Conditions in Pleurotus ostreatus
Mycobiology 31(3): 145-150 (2003) Copyright 2003 by The Koren Society of Mycology Chrcteriztion of Fruitbody Morphology on Vrious Environmentl Conditions in Pleurotus ostretus Kb-Yeul Jng, Chng-Sung Jhune,
More informationConsumer evaluation of cold smoked fat in beef sausages
Interntionl Food Reserch Journl 23(4): 1782-1786 (2016) Journl homepge: http://www.ifrj.upm.edu.my Consumer evlution of cold smoked ft in beef susges Mrtinez, C.C. nd * Mchdo, T.J. Deprtment of Animl,
More informationEffect of AVG Application on Fruit Set, Yield and Fruit Size in Abate Fetel and Packam s Triumph Pears in a Semi-Commercial Statistical Trial
Effect of AVG Appliction on Fruit Set, Yield nd Fruit Size in Ate Fetel nd Pckm s Triumph Pers in Semi-Commercil Sttisticl Tril E. Sánchez nd M. Curetti Instituto Ncionl de Tecnologí Agropecuri Estción
More informationEffects of transglutminase on the quality of white salted noodles made from Korean wheat cultivars
Interntionl Food Reserch Journl 21(1): 195-202 (2014) Journl homepge: http://www.ifrj.upm.edu.my Effects of trnsglutminse on the qulity of white slted noodles mde from Koren whet cultivrs 1 Kng, C. S.,
More informationEffects of Prohexadione-calcium on Grape Yield Components and Fruit and Wine Composition
Effects of Prohexdione-C on Yield nd Composition 73 Effects of Prohexdione-clcium on Grpe Yield Components nd Fruit nd Wine Composition Dnielle Lo Giudice, 1 Tony K. Wolf, 2 * nd Bruce W. Zoecklein 3 Abstrct:
More informationBrassinosteroids Regulate Anthocyanin Biosynthesis in the Ripening of Grape Berries
Brssinosteroids Regulte Anthocynin Biosynthesis in the Ripening of Grpe Berries L.-Y. Lun 1, Z.-W. Zhng 1,2*, Z.-M. Xi 1,2*, S.-S. Huo 1 nd L.-N. M 1 (1) College of Enology, Northwest A&F University, Yngling,
More informationMiniprep - Alkaline Lysis
Miniprep - Alkaline Lysis by A. Untergasser (contact address and download at www.untergasser.de/lab) Version: 1.0 - Print Version (.PDF) ATTENTION: This is a low priced protocol. Use it preferably! 1.
More informationMiniprep - Alkaline Lysis for BACs
Miniprep - Alkaline Lysis for BACs by A. Untergasser (contact address and download at www.untergasser.de/lab) Version: 1.0 - Print Version (.PDF) ATTENTION: This is a low priced protocol. Use it preferably!
More informationProduction of Two Types of Pocket-Forming Flat Bread by the Sponge and Dough Method
Production of Two Types of Pocket-Forming Flt Bred y the Sponge nd Dough Method A. Amr 1,2 nd R. Ajo 1 ABSTRACT Cerel Chem. 82(5):499 503 Two types of flt red (thin nd thick) were produced from strightgrde
More informationEFFECT OF BORON AND ARSENIC ON JUICE ACIDITY
SMITH: BORON & ARSENIC ON GRAPEFRUIT 95 Fig. 2. Vlenci ornge leves from declining tree showing type of mteril used for electron microscopic study Ar rows point to typicl res long midveins tht were excised,
More informationby high-performance liquid chromatography
Journl of Chromtogrphy A, 922 (2001) 359 363 www.elsevier.com/ locte/ chrom Short communiction Anlysis of phenolic constituents of biologicl interest in red wines q by high-performnce liquid chromtogrphy
More informationCluster-zone leaf removal refers to deliberate removal of selected
Lef Removl s Influence on Pinot Noir Bsl lef removl chnges chemicl nd voltile compositions By Hui Feng, Fng Yun, Ptrici A. Skinkis nd Michel C. Qin Cluster-zone lef removl refers to delierte removl of
More informationInfluence of Gibberellic Acid (GA 3 ) on Fruit Quality of Sweet Cherries John Cline
Influence of Gibberellic Acid (GA 3 ) on Fruit Qulity of Sweet Cherries John Cline University of Guelph, Simcoe & Vinelnd Cmpuses Tel: 519-426-7127 Ext 331 Jcline@uoguelph.c 1 Presenttion Outline Introduction
More informationResearch Note Effect of Volume and Toast Level of French Oak Barrels (Quercus petraea L.) on Cabernet Sauvignon Wine Characteristics
Reserch Note Effect of Volume nd Tost Level of French Ok Brrels (Quercus petre L.) on Cernet Suvignon Wine Chrcteristics Pedro Rodríguez-Rodríguez 1 nd Encrn Gómez-Plz 1 * Astrct: The effects of tost level
More informationThe determination of iron in grapes and their alcoholic
OLALLA ET AL.: JOURNAL OF AOAC INTERNATIONAL VOL. 83, NO. 1, 2000 189 RESIDUES AND TRACE ELEMENTS Optimized Determintion of Iron in Grpe Juice, Wines, nd Other Alcoholic Beverges by Atomic Absorption Spectrometry
More informationArchived at
Archived t http://orgprints.org/13657/ Results from three yer testing project of new strwberry cultivrs in Verticillium infested soils nd under orgnic frming conditions A. Spornberger 1, H. Weissinger
More informationQuantification of Glycosidase Activity in Selected Strains of Brettanomyces bruxellensis and Oenococcus oeni
Quntifiction of Glycosidse Activity 303 Quntifiction of Glycosidse Activity in Selected Strins of Brettnomyces ruxellensis nd Oenococcus oeni Ann K. Mnsfield, 1 Bruce W. Zoecklein, 2 * nd Roert S. Whiton
More informationOptimized wine quality potential through fruit-zone management practices in red varieties
Finl Progress Report Virgini Wine Bord, 20 July 2016 Optimized wine qulity potentil through fruit-zone mngement prctices in red vrieties Principl Investigtor: Tony K. Wolf AHS Jr. AREC 595 Lurel Grove
More informationInfluence of Soil Salinity on Sensory Characteristics and Volatile Aroma Compounds of Nero d Avola Wine
Influence of Soil Slinity on Sensory Chrcteristics nd Voltile Arom Compounds of Nero d Avol Wine Antonio Sccco, 1 Antonell Verzer, 2 * Crmel M. Lnz, 1 Antonio Sprcio, 3 Giuseppe Genn, 3 Slvtore Rimondi,
More informationLACTIC ACID BACTERIA (OIV-Oeno , Oeno )
LACTIC ACID BACTERIA (OIV-Oeno 328-2009, Oeno 494-2012) 1. OBJECT, ORIGIN AND FIELD OF APPLICATION Lactic acid bacteria are used in oenology to perform malolactic fermentation. The lactic acid bacteria
More informationThe effects of UV-C treatment on the quality of orange, carrot and celery juice blend
Review Article http://www.lliedcdemies.org/journl-food-science-nutrition/ The effects of UV-C tretment on the qulity of ornge, crrot nd celery juice blend Nik Nornis, Chndrn Somsundrm*, Zulin Rzli Biotechnology
More informationWorm Collection. Prior to next step, determine volume of worm pellet.
Reinke Lab ChIP Protocol (last updated by MK 05/24/13) Worm Collection 1. Collect worms in a 50ml tube. Spin and wait until worms are collected at the bottom. Transfer sample to a 15ml tube and wash with
More informationEffect of Phosphate Salts on the Pasting Properties of Korean Instant-Fried Noodle
Effect of Phosphte Slts on the Psting Properties of Koren Instnt-Fried Noodle Li Wng, 1,2 Gry G. Hou, 2,3 Y-Hsun Hsu, 2 nd Lirong Zhou 4 ABSTRACT Cerel Chem. 88(2):142 146 Up to 15% modified potto strch
More informationOxidation treatments affecting Sauvignon blanc wine sensory and chemical composition
Oxidtion tretments ffecting Suvignon blnc wine sensory nd chemicl composition by Crien Coetzee Disserttion presented for the degree of Doctor of Philosophy (Agriculturl Science) t Stellenbosch University
More informationBiological Control of Alternaria Fruit Rot of Chili by Trichoderma Species under Field Conditions
Mycobiology 38(2) : 113-117 (2010) The Koren Society of Mycology DOI:10.4489/MYCO.2010.38.2.113 Biologicl Control of Alternri Fruit Rot of Chili by Trichoderm Species under Field Conditions Most. Ferdousi
More informationThe effect of seed treatments on the yield and yield components of various levels of sprouted wheat
Brnrd nd Booyse (2018). Seed Science nd Technology, 46, 1, 53-63. https://doi.org/10.15258/sst.2018.46.1.05 The effect of seed tretments on the yield nd yield components of vrious levels of sprouted whet
More informationArticle. Discrimination of Commercial Roasted and Ground Coffees According to Chemical Composition. Romilaine M. N. de Souza and Marta T.
Article J. Brz. Chem. Soc., Vol. 23, No. 7, 1347-1354, 2012. Printed in Brzil - 2012 Sociedde Brsileir de Químic 0103-5053 $6.00+0.00 A Discrimintion of Commercil Rosted nd Ground Coffees According to
More informationTHE EFFECT OF INSTANT ARABICA COFFEE ON THE VIABILITY OF LACTOBACILLUS ACIDOPHILUS AND BIFIDOBACTERIUM BIFIDUM IN A PROBIOTIC, LACTO- FREE DESSERT
THE EFFECT OF INSTANT ARABICA COFFEE ON THE VIABILITY OF LACTOBACILLUS ACIDOPHILUS AND BIFIDOBACTERIUM BIFIDUM IN A PROBIOTIC, LACTO- FREE DESSERT G.Durte, 1,2 C. O. Fernndes 1, M.M. Lemos, 3 I. Felberg,
More informationEvaluation of Grey Forecasting Method of Total Domestic Coffee Consumption in Indonesia
Interntionl Journl of Business nd Economics Reserch 2017; 6(4): 67-72 http://www.sciencepublishinggroup.com/j/ijber doi: 10.11648/j.ijber.20170604.15 ISSN: 2328-7543 (Print); ISSN: 2328-756X (Online) Evlution
More informationA simple method for the quantitative analysis of tyrosol by hplc in liquid Czapek Cultures from endophytic fungi
Universidde de São Pulo Bibliotec Digitl d Produção Intelectul - BDPI Deprtmento de Físic e Químic - FCFRP/DFQ Artigos e Mteriis de Revists Científics - FCFRP/DFQ 2009 A simple method for the quntittive
More information*Joseph Adubofuor, Michael Amoafo Mensah and Sylvia Dabri
Africn Journl of Food Science nd Technology (ISSN: 2141-5455) Vol. 3(10) pp. 244-251, December, 2012 Avilble Online http://www.interesjournls.org/ajfst Copyright 2012 Interntionl Reserch Journls Full Length
More informationEffect of Whitener Type and Paddy Moisture Content on Rice Grain Damage During Milling Process
Americn-Eursin J. Agric. & Environ. Sci., (3): 470-474, 2011 ISSN 1818-6769 IDOSI Publictions, 2011 Effect of Whitener Type nd Pddy Moisture Content on Rice Grin Dmge During Milling Process 1 2 Seed Firouzi
More informationEarly feeding practices seem to have no impact on the risk of developing celiac disease during childhood
FOCUS Erly feeding prctices seem to hve no impct on the risk of developing celic disese during childhood Primry Prevention of Celic Disese: Environmentl Fctors with Focus on Erly Nutrition by Ann Chmielewsk
More information2006 BELL PEPPER VARIETY EVALUATION TRIALS
26 BELL PEPPER VARIETY EVALUATION TRIALS In Sn Joquin County University of Cliforni Coopertive Extension 42 South Wilson Wy Stockton, CA 9525 1 26 BELL PEPPER VARIETY EVALUATION TRIAL In Sn Joquin County
More informationEffect of oak barrel type on the volatile composition of wine: Storage time optimization
LWT 39 (26) 199 25 www.elsevier.com/locte/lwt Effect of ok rrel type on the voltile composition of wine: Storge time optimiztion Teres Grde Cerd n, Crmen Ancı n-azpilicuet Deprtment of Applied Chemistry,
More informationRelationship between fermentation behaviour, measured with a 3D vision Structured Light technique, and the internal structure of bread.
Reltionship between fermenttion behviour, mesured with 3D vision Structured Light technique, nd the internl structure of bred. Smuel Verdú 1*, Eugenio Ivorr 2, Antonio J. Sánchez 2, Jose M. Brt 1, Rúl
More informationAbstract. Ramiréz-Aristizabal, L. S. * ; Ortiz, A. ; Restrepo-Aristizabal, M.F.; Salinas-Villada, J.F. Universidad Tecnológica de Pereira,Colombia
Evlution of the ntioxidnt cpcity nd content of polyphenols obtined from te (Cmelli sinensis) of four brnds sold in Colombi by extrction t room temperture Rmiréz-Aristizbl, L. S. * ; Ortiz, A. ; Restrepo-Aristizbl,
More informationRudd Report. Are Fast-Food Restaurants Keeping Their Promises to Offer Healthier Kids Meals? Summary. August 2017
Rudd Report UCONNRUDDCENTER.ORG August 2017 Are Fst-Food Resturnts Keeping Their Promises to Offer Helthier Kids Mels? AUTHORS: Jennifer Hrris, PhD, MBA Mi Hyry, MPA Nicole Seymour, MSW Yoon-Young Choi,
More informationCERTIFIED CLONE AND POWDERY MILDEW IMPACT ROTUNDONE IN DURAS WINES
Olivier GEFFROY *, Olivier YOBRÉGAT, Thierry DUFOURCQ, Trcey SIEBERT,Éric SERRANO IFV Pôle Sud-Ouest, V innopôle, BP, 80 Lisle sur Trn Frnce IFV Pôle Sud-Ouest, Domine de Mons, 00 Cussens Frnce The Austrlin
More informationTemporal variation in temperature and rainfall differentially affects ectomycorrhizal colonization at two contrasting sites
New Phytol. (1998), 139, 733 739 Temporl vrition in temperture nd rinfll differentilly ffects ectomycorrhizl coloniztion t two contrsting sites BY RANDY L. SWATY*, CATHERINE A. GEHRING, MATT VAN ERT, TAD
More informationEnsiling characteristics and aerobic stability of temperate grasses containing different concentrations of water soluble carbohydrates
REPORT TO THE STAPLEDON MEMORIAL TRUST Ensiling chrcteristics nd eroic stility of temperte grsses contining different concentrtions of wter solule crohydrtes Aner A. Rodríguez-Crís Deprtment of Animl Science,
More informationRelationship Between Microclimatic Data, Aroma Component Concentrations and Wine Quality Parameters in the Prediction of Sauvignon blanc Wine Quality
Reltionship Between Microclimtic Dt, Arom omponent oncentrtions nd Wine Qulity Prmeters in the Prediction of Suvignon blnc Wine Qulity J. Mris, F. litz 2 nd P. D. Hsbroek3 1) AR lnfruiiec-nietvoorbij,
More informationEFFECT OF DISSOLVED OXYGEN AND DEOXYGENATION ON THE QUALITY OF ORANGE JUICE
EFFECT OF DISSOLVED OXYGEN AND DEOXYGENATION ON THE QUALITY OF ORANGE JUICE By ROSALIA GARCIA TORRES A DISSERTATION PRESENTED TO THE GRADUATE SCHOOL OF THE UNIVERSITY OF FLORIDA IN PARTIAL FULFILLMENT
More informationCoffee Berry Processing By-Product Valorization: Coffee Parchment as a Potential Fiber Source to Enrich Bakery Goods
Reserch Article imedpub Journls www.imedpub.com Journl of Food, Nutrition nd Popultion Helth Coffee Berry Processing By-Product Vloriztion: Coffee Prchment s Potentil Fiber Source to Enrich Bkery Goods
More informationMALTING CONDITIONS FOR EVALUATION OF RYE CULTIVARS
MALTING CONDITIONS FOR EVALUATION OF RYE CULTIVARS A Thesis Submitted to the Grdute Fculty of the North Dkot Stte University of Agriculture nd Applied Science By Yujun Wng In Prtil Fulfillment of the Requirements
More informationJournal of Food Engineering
Journl of Food Engineering 97 (1) 135 143 Contents lists vilble t ScienceDirect Journl of Food Engineering journl homepge: www.elsevier.com/locte/jfoodeng Noodle qulity ffected by different cerel strches
More informationAn Economic And Simple Purification Procedure For The Large-Scale Production Of Ovotransferrin From Egg White
An Economic And Simple Purification Procedure For The Large-Scale Production Of Ovotransferrin From Egg White D. U. Ahn, E. J. Lee and A. Pometto Department of Animal Science, Iowa State University, Ames,
More informationSurveillance and outbreak reports
Surveillnce nd outbrek reports Outbrek of slmonellosis fter wedding prty, Bvri, Germny, summer 2010: the importnce of implementing food sfety concepts B von Wissmnn (betrix.wissmnn@gmil.com) 1,2, C Klinc
More informationEffects of guar gum and arabic gum on the physicochemical, sensory and flow behaviour characteristics of frozen yoghurt
doi: 1.1111/j.1471-37.211.75.x ORIGINAL RESEARCH Effects of gur gum nd ric gum on the physicochemicl, sensory nd flow ehviour chrcteristics of frozen yoghurt RAHIL REZAEI, MORTEZA KHOMEIRI,* MAHDI KASHANINEJAD
More informationAlcohol Dehydrogenase Activities of Wine Yeasts in Relation
APPLID AND NVIRONMNTAL MICROBIOLOGY, Nov. 1976, p. 666-67 Copyright 1976 Americn Society for Microbiology Vol. 32, No. 5 Printed in U.S.A. Alcohol Dehydrogense Activities of Wine Yests in Reltion to Higher
More informationAgronomic approaches in yield and quality stability of high oleic sunflowers (Helianthus annuus L.)
Agronomic pproches in yield nd qulity stbility of high oleic sunflowers (Helinthus nnuus L.) to obtin the Ph. D. degree in the Fculty of Agriculturl Sciences, Georg-August-University Göttingen, Germny
More informationCentral NC Organic Bell and Hot Pepper Variety Trial, Summer 2014
Ojective Centrl NC Orgnic Bell nd Hot Pepper Vriety Tril, Summer 2014 North Crolin A&T Stte University John Beck, Aron Moore, nd Dr. Snjun Gu NC A&T Frm, Greensoro, NC To evlute the performnce of some
More informationModeling Impacts of Viticultural and Environmental Factors on 3-Isobutyl-2-Methoxypyrazine in Cabernet franc Grapes
Modeling Impcts of Viticulturl nd Environmentl Fctors on 3-Isobutyl-2-Methoxypyrzine in Cbernet frnc Grpes Justin J. Scheiner, 1 Justine E. Vnden Heuvel, 2 Bruce Pn, 3 nd Gvin L. Scks 4 * Abstrct: A study
More informationMem. Faculty. B. O. S. T. Kindai University No. 38 : 1 10 (2016)
Mem. Faculty. B. O. S. T. Kindai University No. 38 : 1 10 (2016) 1 2 Memoirs of The Faculty of B. O. S. T. of Kindai University No. 38 2016 In recent years, several papers were published on microflora
More informationLactic acid bacteria associated with wine grapes from several Australian vineyards
Journal of Applied Microbiology ISSN 1364-5072 ORIGINAL ARTICLE Lactic acid bacteria associated with wine grapes from several Australian vineyards S. Bae, G.H. Fleet and G.M. Heard Food Science and Technology,
More informationMaxiprep - Alkaline Lysis
Maxiprep - Alkaline Lysis by A. Untergasser (contact address and download at www.untergasser.de/lab) Version: 1.0 - Print Version (.PDF) ATTENTION: This is a low priced protocol. Use it preferably! 1.
More informationIdentification and Classification of Pink Menoreh Durian (Durio Zibetinus Murr.) Based on Morphology and Molecular Markers
RESEARCH Identification and Classification of Pink Durian (Durio Zibetinus Murr.) Based on Morphology and Molecular Markers Nandariyah a,b * adepartment of Agronomy, Faculty of Agriculture, Sebelas Maret
More informationGarlic Sprouts Grown Indoors at Kitchen Sites
Medicinl nd Aromtic Plnt Science nd Biotechnology 008 Globl Science Books Grlic Sprouts Grown Indoors t Kitchen Sites FeiFei Qin,,3* HuiLin Xu Gng M 4 Interntionl Nture Frming Reserch Center, Ht, Ngno,
More information