EUREKA project 5885! SQAH /Czech-English cooperation /

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EUREKA project 5885! SQAH /Czech-English cooperation 2011-2014/ Development of sustainable quality aroma dwarf hops in both the CR and the U.K. to supply brewing worldwide Vyšlechtění jemných aromatických odrůd českého a anglického chmele vhodných pro pěstování na nízkých konstrukcích s celosvětovým uplatněním při výrobě kvalitního piva Ing. Vladimír Nesvadba, Ph.D. a Ing. Josef Vostřel, CSc. Hop Research Institute Ltd., Žatec

Project partners Czech Republic Hop Research Institute Ltd., Žatec Vladimír Nesvadba, Josef Vostřel United Kingdom Philip Davies & Son, Dormington, Hereford Peter Glendinning Wye Hops Limited, Canterbury, Kent Peter Darby

Main advantages 1. Very large reduction of the demand of casual labor 2. Significant reduction of agrochemicals lower environmental impact 3. Harvesting is performed by a mobile picking machine offering more flexible operation and producing higher quality crop.

Activities 1. Selection of perspective parental components 2. Assessment of progenies and selection of the best genotypes 3. Assessment of the best genotypes in field trials 4. Registration trials

Researchers and technicians involved Necessary equipment: Glasshouses, hop gardens, laboratories, pilot brewery Research team 17 empoloyees (5 Dept.)): Breeding Nesvadba, Polončíková, Henychová, Hervert, Krejčíková, Oppelová, Špánková Hop protection Vostřel, Zahrádková Molecular biology Patzak, Henychová Chemistry Krofta, Vrabcová, Mravcová Agricultural techn. Ježek, Kozlovský, Karban Area: 2011 6 NK = 3.5 ha 2012 8 NK = 4.9 ha 2013 8 NK = 5.1 ha 2014 9 NK = 6.3 ha 55,000 seeds 28,000 seedlings.

Aroma genotypes H Row Field Plant Alpha acids (% w.w.) Beta Acids (% hm). Ratio Alpha/Beta Cohumulone (% rel.) Colupulone (% rel.) X DMX 18 5 1 1 4.7 4.6 1.0 42.5 69.7 0.41 0.08 16 2 5 6 4.1 3.3 1.2 26.9 46.8 0.26 0.16 18 4 5 2 6.5 4.7 1.4 19.3 37.5 0.28 0.10 N25 ANK1109 5.2 3.7 1.4 22.4 40.1 0.31 0.11 18 3 7 11 6.1 4.2 1.4 16.7 37.5 0.26 0.13 16 9 7 8 4.4 3.0 1.5 22.9 41.1 0.25 0.09 18 6 13 5 7.8 5.3 1.5 18.0 37.6 0.49 0.11 16 8 17 9 6.8 4.5 1.5 24.4 44.3 0.31 0.18 16 14 2 3 3.5 2.3 1.5 23.9 40.4 0.23 0.10

Business trips to England

Hop crossing new progenies

Assessment of perspective genotypes

PG 1428

N3

N5

Results of Breeding Work 2009 2014 Applying for11 genotypes into registration trials 5 in 2013 6 in 2014

Brewing tests d

1. Series Krušovice I II III IV V VI Hořkos t - Intenz ita 4,0 3,5 C elková obliba 3,0 2,5 Hořkos t - K valita 1. Series Alpha Points Order H 15 2r 8f 1p 3.8 40 3 H 20 12r 11f 16p 5.1 44 1 SAAZER Os.cl.31 4.0 42 2 H 21 8r 2f 5p 8.9 28 3 NK R 5380 5.1 43 2 Rubin 11.6 46 1 Aroma - K valita 2,0 A roma - Intenz ita Hops I II III IV V VI Intensity of Bitterness 2.69 2.88 2.56 2.75 3.19 3,75 Quality of Bitterness 3.19 3.56 3.88 3.06 3.13 2,88 Intensity of Aroma 3.25 3.31 3.31 3.38 3.31 3,75 Quality of Aroma 2.75 2,63 3.25 3.06 3.50 2,88 Deliciousness 2.56 2.75 3.31 2.69 3.00 2,63 Order (total) 41 35 20 28 21 23 Order 6 5 1 4 2 3

2. Series Krušovice 1 2 3 K Hořkos t - Intenz ita 4,0 3,5 C elková obliba 3,0 2,5 2,0 1,5 Hořkos t - K valita 3. Series Alpha Point Order H 20 11r 1f 2p 4.69 66 3 H 21 2r 14f 15p 4.4 71 1 H 21 3r 7f 7 p 4.87 70 2 Saazer 3.98 62 4 Aroma - Kvalita Aroma - Intenz ita Hops 1 2 3 K Intensity of Bitterness 3.43 3.64 3.64 3.36 Quality of bitterness 3.21 2.86 2.93 3.36 Intensity of Aroma 2,42 1,92 2,33 2.25 Quality of Aroma 3.50 3.17 3.25 3.00 Deliciousness 3.14 3.21 3.29 2.93 Order (total) 18 17 16 19 Order 3 2 1 4

3. Series Pilsen Taste Sample 1 Sample 2 Sample 3 Sample 4 SWEET 4.2 4.0 4.0 4.0 BITTER 5.7 5.8 6.0 6.7 BODY 5.3 5.8 5.8 5.6 ESTERY / AROMA 2.0 2.0 2.0 2.0 ASTRINGENT 3.2 3.2 3.0 2.5 CARAMEL 1.7 1.5 1.5 1.3 3. Series Alp ha Poin ts Order H 20 11r 1f 2p 4.69 66 3 H 21 2r 4f 15p 4.40 71 1 H 21 3r 7f 7 p 4.87 70 2 Saazer 3.98 62 4 BURNT 1.2 1.0 1.7 1.5 MALTY 2.8 2.8 3.0 3.0 DIACETYL 1.8 1.8 2.0 2.2 HOPPY 3.7 4.3 3.5 4.5 KETTLE HOP 1.5 2.0 1.7 2.0 HOP OIL 1.3 1.8 1.3 1.8 RESINOUS 0.2 FRESHLY CUT GRASS 0.3 1.2 0.5 0.2 STRAWLIKE 0.2 0.2 0.3 0.2 ISOVALERIC 0.3 0.2 0.2 0.3 AUTOLYSED 0.8 SOUR 0.7 0.5 0.7 0.5 DRINKABILITY RATING 6.9 7.4 6.3 8.3

Presentation of the Results 1. IHGC Scientific Commission, Lublin, Poland 2011 Proceedings. 2. New knowledge from genetics and breeding of agricultural crops. Piešťany, Slovakia, 2011 Proceedings. 3. American Hop Convention 2012 Hop Research Council CA, USA. 4. IHGC Scientific Commission, Kiev, Ukraine, 2013 Proceedings. 5. ISHS, Third International Humulus Symposium, Acta Horticulturae, Zatec, Czech Republic 2013 Proceedings. 6. Publications in scientific and technical journals. 7. Workshop: Use of new perspective hop genotypes in brewing industry. 8. Experimental hop gardens planted with perspective genotypes. 9. Applying for registration trials 11 genotypes their assertion in breweries.

Practical Results after Finishing the Project 1. Till 2018 the first Czech Dwarf Varieties (EUREKA, Saaz EUREKA) will be released (Plant Variety Rigths Protection). 2. Growing of the New Dwarf Varieties in Practice and their Use in Brewing Industry. New Types of Beer Eureka Lager (Ale) 3. Further Cooperation with English Researchers (Commercialisation of Highly Aroma European hops by both the Czech Republic and the U.K. to supply brewing demand worldwide).

Thank you for your attention!