Date : March 29, 2018 CERTIFICATE OF ANALYSIS - GC PROFILING SAMPLE IDENTIFICATION Internal code : 18C20-ALZ3-1-CC Customer identification : Rosemary Type : Essential oil Source : Rosmarinus officinalis ct. 1,8-Cineole Customer : Organic Aromas ANALYSIS Method: PC-PA-014-17J19 - Analysis of the composition of an essential oil, or other volatile liquid, by FAST GC-FID (in French); identifications validated by GC-MS. Analyst : Sylvain Mercier, M. Sc., Chimiste Analysis date : March 28, 2018 Checked and approved by : Alexis St-Gelais, M. Sc., chimiste 2013-174 Note: This report may not be published, including online, without the written consent from Laboratoire PhytoChemia. This report is digitally signed, it is only considered valid if the digital signature is intact. Page 1/9
PHYSICOCHEMICAL DATA Physical aspect: Clear liquid Refractive index: 1.4660 ± 0.0003 (20 C) CONCLUSION No adulterant, contaminant or diluent has been detected using this method. Page 2/9
ANALYSIS SUMMARY Identification DB-5 (%) DB-WAX (%) Classe Isovaleral tr tr Aliphatic aldehyde Toluene tr 13.64* Simple phenolic (2E)-Hexenal tr 0.01 Aliphatic aldehyde (3Z)-Hexenol tr 0.01 Aliphatic alcohol Hexanol tr tr Aliphatic alcohol Hashishene 0.01 [13.64]* Monoterpene Tricyclene 0.13 0.13 Monoterpene α-thujene 0.09 0.09 Monoterpene α-pinene 13.61 [13.64]* Monoterpene Camphene 4.71* 4.29 Monoterpene α-fenchene [4.71]* 0.41 Monoterpene Thuja-2,4(10)-diene 0.03 0.11* Monoterpene Sabinene 9.68* [0.11]* Monoterpene β-pinene [9.68]* 9.59 Monoterpene Octen-3-ol 0.08* 0.06* Aliphatic alcohol Octan-3-one [0.08]* 0.02 Aliphatic ketone Myrcene 0.70 0.71 Monoterpene Octan-3-ol 0.13* tr Aliphatic alcohol α-phellandrene [0.13]* 0.11 Monoterpene Pseudolimonene [0.13]* 0.02 Monoterpene Δ3-Carene 0.03 0.03 Monoterpene α-terpinene 0.19 0.19 Monoterpene para-cymene 50.92* 1.04 Monoterpene β-phellandrene [50.92]* 1.86 Monoterpene 1,8-Cineole [50.92]* 46.77 Monoterpenic ether Limonene [50.92]* 1.28 Monoterpene (Z)-β-Ocimene 0.04 0.04 Monoterpene (E)-β-Ocimene 0.02 0.02 Monoterpene γ-terpinene 0.51 0.52 Monoterpene cis-sabinene hydrate 0.03 0.02 Monoterpenic alcohol Isoterpinolene tr tr Monoterpene Terpinolene 0.11 0.12 Monoterpene para-cymenene 0.03 0.03 Monoterpene trans-sabinene hydrate 0.01 0.01 Monoterpenic alcohol Linalool 0.39 0.41 Monoterpenic alcohol endo-fenchol 0.03 0.03 Monoterpenic alcohol cis-para-menth-2-en-1-ol 0.02 0.01 Monoterpenic alcohol Camphor 12.28* 12.36* Monoterpenic ketone trans-pinocarveol [12.28]* 0.05 Monoterpenic alcohol Camphene hydrate 0.04 1.35* Monoterpenic alcohol Isoborneol 0.01 0.13* Monoterpenic alcohol Pinocarvone 0.02 0.03 Monoterpenic ketone Borneol 1.71 2.59* Monoterpenic alcohol δ-terpineol 0.19 0.15 Monoterpenic alcohol Terpinen-4-ol 0.27 0.27 Monoterpenic alcohol para-cymen-8-ol 0.02 0.03 Monoterpenic alcohol α-terpineol 0.84* [2.59]* Monoterpenic alcohol Myrtenal [0.84]* 0.01 Monoterpenic aldehyde Page 3/9
Myrtenol 0.03 0.03 Monoterpenic alcohol Verbenone 0.06* 0.07* Monoterpenic ketone Unknown [0.06]* tr Unknown Carvone 0.01 0.02 Monoterpenic ketone Bornyl acetate 0.40 0.41 Monoterpenic ester α-cubebene 0.01 [0.06]* Sesquiterpene α-ylangene 0.03 0.03 Sesquiterpene α-copaene 0.07 [12.36]* Sesquiterpene Methyleugenol 0.01 0.01 Phenylpropanoid β-caryophyllene 1.35 [1.35]* Sesquiterpene β-copaene 0.01 0.01 Sesquiterpene Aromadendrene 0.02 0.02 Sesquiterpene α-humulene 0.14 [0.13]* Sesquiterpene γ-muurolene 0.06 0.06 Sesquiterpene α-amorphene 0.01 [0.07]* Sesquiterpene β-selinene 0.03 0.01 Sesquiterpene α-selinene 0.03 0.02 Sesquiterpene α-muurolene 0.03 0.03 Sesquiterpene β-bisabolene 0.03 0.04 Sesquiterpene γ-cadinene 0.04 0.03 Sesquiterpene δ-cadinene 0.10* 0.09 Sesquiterpene trans-calamenene [0.10]* 0.02 Sesquiterpene trans-cadina-1,4-diene 0.01 0.02 Sesquiterpene α-calacorene 0.01 0.02 Sesquiterpene Caryophyllene oxide isomer 0.06* 0.01 Sesquiterpenic ether Caryophyllene oxide [0.06]* 0.06 Sesquiterpenic ether Humulene epoxide II 0.01 0.01 Sesquiterpenic ether Total identified 99.43% 99.56% *: Two or more compounds are coeluting on this column [xx]: Duplicate percentage due to coelutions, not taken account in the identified total tr: The compound has been detected below 0.005% of total signal. Note: no correction factor was applied Page 4/9
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FULL ANALYSIS DATA Identification Column DB-5 Column DB-WAX R.T R.I % R.T R.I % Isovaleral 0.59 639 tr 0.79 884 tr Toluene 1.13 754 tr 1.49* 999 13.64 (2E)-Hexenal 1.97 846 tr 3.58 1178 0.01 (3Z)-Hexenol 2.07 854 tr 5.90 1345 0.01 Hexanol 2.23 867 tr 5.55 1320 tr Hashishene 2.79 912 0.01 1.49* 999 [13.64] Tricyclene 2.83 915 0.13 1.34 976 0.13 α-thujene 2.94 923 0.09 1.53 1003 0.09 α-pinene 3.04 929 13.61 1.49* 999 [13.64] Camphene 3.22* 941 4.71 1.83 1031 4.29 α-fenchene 3.22* 941 [4.71] 1.76 1024 0.41 Thuja-2,4(10)- diene 3.31 947 0.03 2.42* 1088 0.11 Sabinene 3.66* 970 9.68 2.42* 1088 [0.11] β-pinene 3.66* 970 [9.68] 2.26 1072 9.59 Octen-3-ol 3.88* 985 0.08 6.93* 1419 0.06 Octan-3-one 3.88* 985 [0.08] 4.13 1219 0.02 Myrcene 3.96 990 0.70 3.03 1136 0.71 Octan-3-ol 4.10* 1000 0.13 6.20 1367 tr α-phellandrene 4.10* 1000 [0.13] 2.95 1130 0.11 Pseudolimonene 4.10* 1000 [0.13] 2.99 1133 0.02 Δ3-Carene 4.18 1005 0.03 2.74 1114 0.03 α-terpinene 4.30 1012 0.19 3.13 1144 0.19 para-cymene 4.57* 1029 50.92 4.28 1229 1.04 β-phellandrene 4.57* 1029 [50.92] 3.40 1164 1.86 1,8-Cineole 4.57* 1029 [50.92] 3.55 1176 46.77 Limonene 4.57* 1029 [50.92] 3.39 1163 1.28 (Z)-β-Ocimene 4.71 1038 0.04 3.96 1206 0.04 (E)-β-Ocimene 4.86 1048 0.02 4.16 1221 0.02 γ-terpinene 4.98 1055 0.51 4.00 1209 0.52 cis-sabinene hydrate 5.13 1065 0.03 7.06 1429 0.02 Isoterpinolene 5.38 1080 tr 4.42 1239 tr Terpinolene 5.43 1084 0.11 4.47 1243 0.12 para-cymenene 5.46 1085 0.03 6.50 1388 0.03 trans-sabinene hydrate 5.62 1095 0.01 8.11 1507 0.01 Linalool 5.73 1102 0.39 8.21 1515 0.41 endo-fenchol 5.89 1113 0.03 8.54 1540 0.03 cis-para-menth- 2-en-1-ol 6.01 1120 0.02 8.28 1520 0.01 Camphor 6.29* 1138 12.28 7.42* 1456 12.36 trans- Pinocarveol 6.29* 1138 [12.28] 9.33 1601 0.05 Camphene hydrate 6.33 1141 0.04 8.67* 1550 1.35 Isoborneol 6.49 1151 0.01 9.53* 1618 0.13 Pinocarvone 6.54 1154 0.02 8.09 1505 0.03 Page 8/9
Borneol 6.68 1163 1.71 9.94* 1651 2.59 δ-terpineol 6.71 1165 0.19 9.63 1626 0.15 Terpinen-4-ol 6.84 1173 0.27 8.74 1556 0.27 para-cymen-8-ol 7.03 1186 0.02 11.78 1804 0.03 α-terpineol 7.08* 1189 0.84 9.94* 1651 [2.59] Myrtenal 7.08* 1189 [0.84] 8.88 1566 0.01 Myrtenol 7.15 1193 0.03 11.01 1739 0.03 Verbenone 7.27* 1201 0.06 9.76* 1637 0.07 Unknown [m/z 95, 93 (32), 121 (24), 79 (22), 91 7.27* 1201 [0.06] 11.17 1753 tr (21), 105 (16)... 154 (2)] Carvone 7.82 1238 0.01 10.19 1671 0.02 Bornyl acetate 8.46 1280 0.40 8.44 1533 0.41 α-cubebene 9.38 1344 0.01 6.93* 1419 [0.06] α-ylangene 9.67 1365 0.03 7.30 1447 0.03 α-copaene 9.73 1369 0.07 7.42* 1456 [12.36] Methyleugenol 10.22 1403 0.01 13.47 1956 0.01 β-caryophyllene 10.32 1411 1.35 8.67* 1550 [1.35] β-copaene 10.45 1421 0.01 8.58 1543 0.01 Aromadendrene 10.58 1430 0.02 8.80 1560 0.02 α-humulene 10.77 1445 0.14 9.53* 1618 [0.13] γ-muurolene 11.12 1470 0.06 9.84 1643 0.06 α-amorphene 11.16 1474 0.01 9.76* 1637 [0.07] β-selinene 11.22 1478 0.03 10.12 1665 0.01 α-selinene 11.34 1487 0.03 10.14 1667 0.02 α-muurolene 11.44 1495 0.03 10.28 1678 0.03 β-bisabolene 11.58 1505 0.03 10.39 1687 0.04 γ-cadinene 11.60 1507 0.04 10.65 1708 0.03 δ-cadinene 11.74* 1518 0.10 10.66 1710 0.09 trans- Calamenene 11.74* 1518 [0.10] 11.46 1778 0.02 trans-cadina- 1,4-diene 11.85 1526 0.01 10.89 1729 0.02 α-calacorene 11.96 1535 0.01 12.34 1854 0.02 Caryophyllene oxide isomer 12.44* 1573 0.06 12.91 1905 0.01 Caryophyllene oxide 12.44* 1573 [0.06] 12.99 1912 0.06 Humulene epoxide II 12.78 1599 0.01 13.58 1966 0.01 Total identified 99.43% 99.56% Total reported 99.43% 99.56% *: Two or more compounds are coeluting on this column [xx]: Duplicate percentage due to coelutions, not taken account in the identified total tr: The compound has been detected below 0.005% of total signal. Note: no correction factor was applied R.T.: Retention time (minutes) R.I.: Retention index Page 9/9