ISTA TEZ- Committee
List of Committee Members Chair: Stefanie Krämer* Germany Vice-Chair: Ronald Don* United Kingdom Augusto Martinelli* Argentina Christofer Wood* United Kingdom Anny van Pijlen* Netherland Valerie Blouin* France Sharon Davidson* United States María Belén Aranguren* Argentina Teresita Farras* Uruguay Gary Duffy* Ireland Linda Maile* United Kingdom Irena Jumburga* Latvia Jose B. Franca- Neto* Brazil Izelle Allison South Africa Sergio Pasquini* Italy
A RULES Development Introduction of new species: Table 6 A Part I Lycopersicon completed 2007 Allium completed 2007 Cucurbita completed 2007 Lactuca Glycine max completed 2008 Table 6 A Part II Larix, Picea completed 2008
A: The figures are representitive for Triticum, x Triticosecale, Secale, Hordeum and Avena when prepared by bisection or bisected for evaluation. B: Triticum prepared by excised embryo method. B II is scutellum seen from the back. Figure 6.3 b Evaluation guide for cereals: non-viable seeds I II III IV V A Continuation of this figures for all cereals B
B Publications Reorganisation of Tetrazolium Handbook Creation of a digital version completed 2007 Update of information Inclusion of theoretical background Rearrangement of test methods completed 2009
Old version Genus and Family References for Additional Guidance Prep. of Dry Seed before Moistening Moistening Method and Time Prep. after Moistening Staining Time Prep. for Evaluation Nutritiv e Tissues Evaluation Remarks 1 2 3 4 5 6 7 8 9 10 Abies (T) Pinacea 29,34,38, 40,84,86, 88,113, 124,158, 204 8 h 18 2,6a,i,8j 24-48 9c,d (+) A;D 1
Genus and Family Abies (T) Pinacea Seed anantomy Embryo with endosperm (Nutritive Tissue)
Pretreatment None, or soaking in water at 20 C Preparation before staining Transversal cut longitudinal cut optionel low pressure treatment Staining 18 h Preparation for Evaluation Cut longitudinally through endosperm, expose embryo, remove seed coat
Evaluation (maximum area of unstained, flaccid and/or necrotic tissue permitted) None, except small superficial necrosis on the outer part of the endosperm, n ot in connection with embryo cavity Remarks Old and dry seeds may give more consistent results if imbibed for 48 hours
Continue Tetrazolium Working Sheets Part I Barbarea Bellis perennis Callistephus chinensis Catharanthus Cicer arientinum Cichorium Cirsium Coreopsis grandiflora Coreopsis tinctoria Cosmos Dahlia Foeniculum vulgare Gazania Gentiana lutea Gentiana septemfida Meconopsis Passiflora Perilla Pimpinella Polygonum Quamoclit Rhinanthus Ricinus communis Rudbeckia hirta Salvia Securinega Urtica Valeriana officinalis Valerianella Veronica completed 2006
Continue Tetrazolium Working Sheets Part II Betula Chamaedorea elegans Chimonanthus praecox Citrus spp. Livistona Musa Pachypodium Panax ginseng Phoenix Pittosporum Ruscus Sequoiadendron giganteum Strelitzia Trachycarpus fortunei Yucca Zelkova completed 2008 Revision of ISTA Tetrazolium Working Sheets completed 2007
C Workshops and Seminars 2007 Italy One day theory and praxis of TEZ during the Flowerseed Workshop in Milano 2008? 2009? 2010? Please contact the TEZ Committee if someone is interessted in organizing a workshop or seminar.
D Proficiency Tests 2007 Panicum maximum 2007 Medicago sativa
Building of a slide collection with pictures of viable and non viable seeds? Questionnaire about TEZ -Testing?
Thank you