Date : April 24, 2018 CERTIFICATE OF ANALYSIS - GC PROFILING SAMPLE IDENTIFICATION Internal code : 18D17-HBN9-1-CC Customer identification : Peppermint Oil - India - 98182 Type : Essential oil Source : Mentha x piperita Customer : Health & Beauty Natural Oils ANALYSIS Method: PC-PA-014-17J19 - Analysis of the composition of an essential oil, or other volatile liquid, by FAST GC-FID (in French); identifications validated by GC-MS. Analyst : Sylvain Mercier, M. Sc., Chimiste Analysis date : April 19, 2018 Checked and approved by : Alexis St-Gelais, M. Sc., chimiste 2013-174 Note: This report may not be published, including online, without the written consent from Laboratoire PhytoChemia. This report is digitally signed, it is only considered valid if the digital signature is intact. Page 1/11
PHYSICOCHEMICAL DATA Physical aspect: Faintly yellow liquid Refractive index: 1.4600 ± 0.0003 (20 C) COMPLIANCE WITH NF T 75-210:2008 OIL OF PEPPERMINT (MENTHA X PIPERITA) Compound USA type Other type Min. % Max. % Min. % Max. % Observed % Complies? 3-Octanol 0.1 0.4 0.1 0.5 0.1 Yes 1,8-Cineole 4.0 6.0 3.0 8.0 5.1 Yes Limonene 1.0 2.5 1.0 3.0 2.1 Yes Sabinene hydrate* 0.5 2.3 0.5 2.0 0.1 No Menthone 15.0 25.0 13.0 28.0 19.9 Yes Isomenthone 2.0 4.5 2.0 8.0 3.3 Yes Menthofuran 1.5 6.0 1.0 8.0 2.1 Yes Neomenthol 2.5 4.5 2.0 6.0 3.0 Yes Menthol 36.0 46.0 32.0 49.0 46.4 Other Pulegone 0.5 2.5 0.5 3 0.8 Yes Menthyl acetate 3.0 6.5 2.0 8.0 5.4 Yes β-caryophyllene 1.0 2.5 1.0 3.5 2.7 Other Refractive index 1.459 1.465 1.459 1.465 1.4600 Yes *The AFNOR norms nomenclature for this compound appears to be confounding. The value indicated in the table is for cis-sabinene hydrate, as recommended for this compound by Robert P. Adams. CONCLUSION No adulterant, contaminant or diluent has been detected using this method. The oil marginally does not comply with the ISO standard for non-usa peppermint oil. Page 2/11
ANALYSIS SUMMARY Identification DB-5 (%) DB-WAX (%) Classe Isobutanol tr 1.00* Aliphatic alcohol Isovaleral 0.01 0.01 Aliphatic aldehyde 2-Methylbutyral 0.01 tr Aliphatic aldehyde 2-Ethylfuran tr tr Furan Isoamyl alcohol 0.01 0.01 Aliphatic alcohol 2-Methylbutanol 0.01 0.01 Aliphatic alcohol (3Z)-Hexenol tr tr Aliphatic alcohol Hexanol tr Aliphatic alcohol trans-2,5-diethyltetrahydrofuran 0.02 0.02 Furan Hashishene tr 0.69* Monoterpene α-thujene 0.02 0.02 Monoterpene α-pinene 0.68 [0.69]* Monoterpene Camphene 0.06* 0.03 Monoterpene α-fenchene [0.06]* 0.01 Monoterpene 3-Methylcyclohexanone [0.06]* 0.04* Aliphatic ketone Thuja-2,4(10)-diene 0.01 0.31* Monoterpene Sabinene 1.30* [0.31]* Monoterpene β-pinene [1.30]* [1.00]* Monoterpene Octen-3-ol 0.03 0.04 Aliphatic alcohol Octan-3-one 0.02 0.04* Aliphatic ketone Myrcene 0.16 0.16 Monoterpene Octan-3-ol 0.14 0.15 Aliphatic alcohol α-phellandrene 0.04* 0.02 Monoterpene Pseudolimonene [0.04]* 0.01 Monoterpene Δ3-Carene tr 0.01 Monoterpene α-terpinene 0.10 0.10 Monoterpene para-cymene 0.12 0.12 Monoterpene Limonene 7.28* 2.11 Monoterpene 1,8-Cineole [7.28]* 5.15 Monoterpenic ether (Z)-β-Ocimene 0.07 0.06 Monoterpene (E)-β-Ocimene 0.03 [0.04]* Monoterpene γ-terpinene 0.17 0.18 Monoterpene cis-sabinene hydrate 0.14* 2.11* Monoterpenic alcohol para-mentha-3,8-diene [0.14]* 0.01 Monoterpene cis-linalool oxide (fur.) 0.01 0.02 Monoterpenic alcohol Octanol 0.14 0.15 Aliphatic alcohol Terpinolene 0.08* 0.07 Monoterpene para-cymenene [0.08]* 0.01* Monoterpene trans-sabinene hydrate 0.03 0.02 Monoterpenic alcohol 2-Methylbutyl isovalerate? 0.01 0.01 Aliphatic ester Linalool 0.12 0.14* Monoterpenic alcohol 2-Methylbutyl 2-methylbutyrate 0.02 0.03 Aliphatic ester Amyl isovalerate 0.03 [0.04]* Aliphatic ester β-thujone 0.01 [0.01]* Monoterpenic ketone cis-para-menth-2-en-1-ol 0.02 [0.14]* Monoterpenic alcohol allo-ocimene 0.01* 0.01 Monoterpene Octan-3-yl acetate [0.01]* tr Aliphatic ester Isopulegol 0.15* 5.46* Monoterpenic alcohol Page 3/11
neo-isopulegol [0.15]* [5.46]* Monoterpenic alcohol Menthone 19.92 19.87 Monoterpenic ketone Isomenthone 5.21* 3.34* Monoterpenic ketone Menthofuran [5.21]* [2.11]* Monoterpenic ether neo-menthol 2.97 2.97 Monoterpenic alcohol δ-terpineol 0.03 0.04 Monoterpenic alcohol Terpinen-4-ol 46.73* 0.32* Monoterpenic alcohol Menthol [46.73]* 45.92 Monoterpenic alcohol Isomenthol [46.73] [0.32]* Monoterpenic alcohol α-terpineol 0.61 0.77* Monoterpenic alcohol neoiso-menthol [0.61] 0.32 Monoterpenic alcohol trans-isopiperitenol 0.03 0.03 Monoterpenic alcohol Unknown 0.02 Unknown trans-piperitol 0.02 0.02* Monoterpenic alcohol Unknown 0.04 Unknown Citronellol 0.03 0.22* Monoterpenic alcohol Pulegone 0.81 1.33 Monoterpenic ketone Carvone 0.08 0.08 Monoterpenic ketone Piperitone 0.61 0.62 Monoterpenic ketone Decanol 0.38* [0.22]* Aliphatic alcohol neo-menthyl acetate [0.38]* 0.17 Monoterpenic ester 2-Ethylmenthone? 0.02 Aliphatic ketone Dihydroedulan I 0.03 0.03 Terpenic ether Menthyl acetate 5.37 [5.46]* Monoterpenic ester Dihydroedulan II 0.01 0.01 Terpenic ether Isomenthyl acetate 0.11 0.11 Monoterpenic alcohol Bicycloelemene 0.11 [3.34]* Sesquiterpene Piperitenone 0.01 0.01 Monoterpenic ketone α-cubebene 0.01 0.02 Sesquiterpene Evodone 0.02 0.02 Monoterpenic ketone α-copaene 0.05 0.05 Sesquiterpene β-bourbonene 0.19 0.21 Sesquiterpene 1,5-diepi-β-Bourbonene 0.03 0.03 Sesquiterpene β-elemene 0.10 2.56* Sesquiterpene Unknown 0.03 Sesquiterpene Isocaryophyllene 0.02 [5.46]* Sesquiterpene β-caryophyllene 2.67 [2.56]* Sesquiterpene β-copaene 0.11 0.08 Sesquiterpene trans-α-bergamotene 0.02 [2.56]* Sesquiterpene Isogermacrene D 0.05 0.05 Sesquiterpene α-humulene 0.34 [45.92]* Sesquiterpene Muurola-4,11-diene 0.04 0.04 Sesquiterpene 9-epi-β-Caryophyllene 0.16* [45.92]* Sesquiterpene (E)-β-Farnesene [0.16]* 0.12 Sesquiterpene Germacrene D 0.47 [0.77]* Sesquiterpene Menthalactone 0.02 0.02 Monoterpenic lactone Viridiflorene 0.17* 0.12 Sesquiterpene Bicyclogermacrene [0.17]* 0.10 Sesquiterpene γ-cadinene 0.02 [0.02]* Sesquiterpene δ-cadinene 0.06 0.06 Sesquiterpene (E)-Nerolidol 0.01 0.01 Sesquiterpenic alcohol Spathulenol 0.01 0.03 Sesquiterpenic alcohol Page 4/11
Caryophyllene oxide 0.06* 0.05 Sesquiterpenic ether Caryophyllene oxide isomer [0.06]* 0.01 Sesquiterpenic ether Viridiflorol 0.05 0.05 Sesquiterpenic alcohol Total identified 98.90% 98.15% *: Two or more compounds are coeluting on this column [xx]: Duplicate percentage due to coelutions, not taken account in the identified total tr: The compound has been detected below 0.005% of total signal. Note: no correction factor was applied Page 5/11
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FULL ANALYSIS DATA Identification Column DB-5 Column DB-WAX R.T R.I % R.T R.I % Isobutanol 0.54 621 tr 2.14* 1071 1.00 Isovaleral 0.59 640 0.01 0.79 890 0.01 2-Methylbutyral 0.62 650 0.01 0.77 882 tr 2-Ethylfuran 0.75 697 tr 0.92 916 tr Isoamyl alcohol 0.95 728 0.01 3.51 1180 0.01 2-Methylbutanol 0.97 730 0.01 3.45 1176 0.01 (3Z)-Hexenol 2.06 854 tr 5.80 1347 tr Hexanol 2.25 870 tr trans-2,5- Diethyltetrahydrofuran 2.52 894 0.02 1.59 1017 0.02 Hashishene 2.78 912 tr 1.39* 996 0.69 α-thujene 2.93 923 0.02 1.45 1003 0.02 α-pinene 3.00 928 0.68 1.39* 996 [0.69] Camphene 3.20* 940 0.06 1.73 1031 0.03 α-fenchene 3.20* 940 [0.06] 1.65 1023 0.01 3- Methylcyclohexanone 3.20* 940 [0.06] 4.78* 1265 0.04 Thuja-2,4(10)-diene 3.29 946 0.01 2.32* 1089 0.31 Sabinene 3.61* 969 1.30 2.32* 1089 [0.31] β-pinene 3.61* 969 [1.30] 2.14* 1071 [1.00] Octen-3-ol 3.77 979 0.03 6.81 1420 0.04 Octan-3-one 3.86 985 0.02 4.03* 1216 0.04 Myrcene 3.93 990 0.16 2.92 1136 0.16 Octan-3-ol 4.02 996 0.14 6.14 1371 0.15 α-phellandrene 4.06* 999 0.04 2.82 1129 0.02 Pseudolimonene 4.06* 999 [0.04] 2.87 1132 0.01 Δ3-Carene 4.15 1005 tr 2.63 1114 0.01 α-terpinene 4.26 1012 0.10 3.00 1142 0.10 para-cymene 4.38 1019 0.12 4.16 1225 0.12 Limonene 4.48* 1026 7.28 3.24 1160 2.11 1,8-Cineole 4.48* 1026 [7.28] 3.36 1169 5.15 (Z)-β-Ocimene 4.67 1037 0.07 3.83 1203 0.06 (E)-β-Ocimene 4.82 1047 0.03 4.03* 1216 [0.04] γ-terpinene 4.94 1054 0.17 3.86 1204 0.18 cis-sabinene hydrate 5.06* 1062 0.14 6.98* 1432 2.11 para-mentha-3,8-diene 5.06* 1062 [0.14] 4.26 1232 0.01 cis-linalool oxide (fur.) 5.16 1068 0.01 6.54 1400 0.02 Octanol 5.23 1073 0.14 8.23 1526 0.15 Terpinolene 5.39* 1083 0.08 4.34 1236 0.07 para-cymenene 5.39* 1083 [0.08] 6.40* 1390 0.01 trans-sabinene hydrate 5.55 1093 0.03 8.00 1508 0.02 2-Methylbutyl isovalerate? 5.61 1097 0.01 4.75 1263 0.01 Linalool 5.64 1099 0.12 8.15* 1520 0.14 2-Methylbutyl 2- methylbutyrate 5.72 1104 0.02 4.51 1248 0.03 Amyl isovalerate 5.79 1109 0.03 4.78* 1265 [0.04] Page 9/11
(12)] trans-piperitol 7.27 1204 0.02 10.48* 1705 0.02 Unknown [m/z 146, 145 (94), 43 (72), 99 (41), 81 (29), 115 (25), 86 (24)...] 7.32 1207 0.04 β-thujone 5.87 1114 0.01 6.40* 1390 [0.01] cis-para-menth-2-en-1- ol 5.90 1116 0.02 8.15* 1520 [0.14] allo-ocimene 6.08* 1127 0.01 5.66 1337 0.01 Octan-3-yl acetate 6.08* 1127 [0.01] 5.30 1311 tr Isopulegol 6.28* 1140 0.15 8.20* 1524 5.46 neo-isopulegol 6.28* 1140 [0.15] 8.20* 1524 [5.46] Menthone 6.44 1151 19.92 6.75 1416 19.87 Isomenthone 6.57* 1159 5.21 7.08* 1440 3.34 Menthofuran 6.57* 1159 [5.21] 6.98* 1432 [2.11] neo-menthol 6.61 1161 2.97 8.68 1561 2.97 δ-terpineol 6.68 1166 0.03 9.46 1622 0.04 Terpinen-4-ol 6.85* 1177 46.73 8.65* 1559 0.32 Menthol 6.85* 1177 [46.73] 9.28 1608 45.92 Isomenthol 6.90 1180 [46.73] 8.65* 1559 [0.32] α-terpineol 7.00 1187 0.61 9.83* 1652 0.77 neoiso-menthol 7.01 1187 [0.61] 9.54 1629 0.32 trans-isopiperitenol 7.15 1196 0.03 10.42 1700 0.03 Unknown [m/z 43, 99 (84), 81 (46), 986 (43), 126 (36), 71 (28)... 170 7.24 1202 0.02 Citronellol 7.65 1230 0.03 10.79* 1732 0.22 Pulegone 7.69 1233 0.81 9.02 1587 1.33 Carvone 7.76 1237 0.08 10.04 1670 0.08 Piperitone 7.91 1247 0.61 9.99 1666 0.62 Decanol 8.30* 1274 0.38 10.79* 1732 [0.22] neo-menthyl acetate 8.30* 1274 [0.38] 7.74 1489 0.17 2-Ethylmenthone? 8.37 1278 0.02 Dihydroedulan I 8.49 1286 0.03 7.14 1444 0.03 Menthyl acetate 8.59 1293 5.37 8.20* 1524 [5.46] Dihydroedulan II 8.71 1301 0.01 7.43 1466 0.01 Isomenthyl acetate 8.75 1304 0.11 8.34 1535 0.11 Bicycloelemene 9.15 1332 0.11 7.08* 1440 [3.34] Piperitenone 9.22 1337 0.01 12.09 1843 0.01 α-cubebene 9.26 1340 0.01 6.77 1417 0.02 Evodone 9.33 1345 0.02 12.50 1880 0.02 α-copaene 9.68 1370 0.05 7.18 1447 0.05 β-bourbonene 9.79 1378 0.19 7.52 1472 0.21 1,5-diepi-β- 9.81 1379 0.03 7.48 1470 0.03 Bourbonene β-elemene 9.92 1387 0.10 8.47* 1544 2.56 Unknown [m/z 106, 119 (99), 43 (78), 91 (74), 105 (60), 134 (55)... 204 (19)] 10.09 1399 0.03 Isocaryophyllene 10.16 1404 0.02 8.20* 1524 [5.46] Page 10/11
β-caryophyllene 10.26 1411 2.67 8.47* 1544 [2.56] β-copaene 10.39 1421 0.11 8.40 1539 0.08 trans-α-bergamotene 10.52 1431 0.02 8.47* 1544 [2.56] Isogermacrene D 10.60 1437 0.05 9.05 1590 0.05 α-humulene 10.71 1445 0.34 9.35* 1614 [45.92] Muurola-4,11-diene 10.78 1451 0.04 9.41 1618 0.04 9-epi-β-Caryophyllene 10.86* 1456 0.16 9.35* 1614 [45.92] (E)-β-Farnesene 10.86* 1456 [0.16] 9.56 1631 0.12 Germacrene D 11.08 1473 0.47 9.83* 1652 [0.77] Menthalactone 11.22 1483 0.02 15.95 2211 0.02 Viridiflorene 11.29* 1488 0.17 9.71 1642 0.12 Bicyclogermacrene 11.29* 1488 [0.17] 10.11 1674 0.10 γ-cadinene 11.53 1507 0.02 10.48* 1705 [0.02] δ-cadinene 11.67 1518 0.06 10.46 1704 0.06 (E)-Nerolidol 12.22 1562 0.01 13.84 2003 0.01 Spathulenol 12.30 1568 0.01 14.47 2064 0.03 Caryophyllene oxide 12.36* 1572 0.06 12.83 1910 0.05 Caryophyllene oxide isomer 12.36* 1572 [0.06] 12.75 1902 0.01 Viridiflorol 12.48 1582 0.05 14.05 2024 0.05 Total identified 98.90% 98.15% Total reported 98.98% 98.15% *: Two or more compounds are coeluting on this column [xx]: Duplicate percentage due to coelutions, not taken account in the identified total : Peaks apexes were resolved, but peaks overlapped and were summed for analysis tr: The compound has been detected below 0.005% of total signal. Note: no correction factor was applied R.T.: Retention time (minutes) R.I.: Retention index Page 11/11