1 Sample: Client: Sample: Brambleberry Batch # 10390662 CAS Number 8007-08-7 Type: Ginger (Zingiber officinalis) Essential Oil Conclusion: No adulterants, diluents, or contaminants were detected via this method. X Validated By: Phone: 317-361-5044 Essential Validation Services 747 SW 2nd Ave, Gainesville, FL 32601 E-mail: Adam@essentialvalidationservices.com Summary of Analyses A fresh sample of Ginger (Zingiber officinalis) Essential Oil (10 ul in.5to.5 ml Hexane + ETOH) was prepared and analyzed via split injection GC/EI-MS.
2 Main Compounds Percentages Zingiberene 15.92% Ar-Curcumene 12.68% Camphene 10.66% Geranial 6.78% (E,E)-alpha-Farnesene 4.52% Citral 4.35% 1,8-Cineole 2.94% alpha-pinene 2.65% Geraniol 2.13% Limonene 2.10% Borneol 1.68% 6-Methyl-5-hepten-2-one 1.46% Geranyl acetate 1.18% Monoterpenes Sesquiterpenes Monoterpenols Sesquiterpenols Ketones 21.83% 52.65% 5.85% 4.41% 1.52% Esters Ethers Aldehydes Phenols Other 3.14% 2.94% 6.78% 0.00% 0.36% Monoterpenes Sesquiterpenes Monoterpenols Sesquiterpenols Ketones Esters Ethers Phenols Other
3 RT: 9.08-29.84 100 22.95 119 90 80 22.73 119 70 60 11.14 23.47 69 50 18.60 69 40 30 20 10 0 10.72 13.21 13.29 43 14.85 16.50 95 16.99 59 18.01 41 19.00 95 20.73 69 24.98 69 25.78 59 10 12 14 16 18 20 22 24 26 28 Time (min) NL: 4.29 TIC MS EVS-128 (103906 (Zingiber officinale Bramble (5-18-20
4 Total Percentage 99.48% Peak Number RT Area % Compound Name RI Chemical Family 1 10.39 0.19% Tricyclene 923.20 Monoterpenes 2 10.72 2.65% alpha-pinene 6.10 Monoterpenes 3 11.14 10.66% Camphene 953.30 Monoterpenes 4 11.89 0.27% beta-pinene 977.70 Monoterpenes 5 12.05 1.46% 6-Methyl-5-hepten-2-one 985.90 Ketones 6 12.18 1.12% Myrcene 989.20 Monoterpenes 7 12.59 0.11% alpha-phellandrene 1004.10 Monoterpenes 8 12.75 0.05% delta-3-carene 1011.30 Monoterpenes 9 13.09 0.09% para-cymene 1024.30 Monoterpenes 10 13.21 2.10% Limonene 1029.50 Monoterpenes 11 13.21 Co-Eluting beta-phellandrene 1030.00 Monoterpenes 12 13.29 2.94% 1,8-Cineole 1031.80 Ethers 13 14.66 0.24% Terpinolene 1089.43 Monoterpenes 14 14.85 0.73% Linalool 1099.00 Monoterpenols 15 16.04 0.22% Camphene hydrate 1148.70 Esters 16 16.13 0.06% Camphor 1153.86 Ketones 17 16.50 1.68% Borneol 1168.71 Monoterpenols 18 16.72 0.26% Terpinen-4-ol 1180.57 Monoterpenols 19 16.84 0.06% para-cymen-8-ol 1184.43 Monoterpenols 20 16.99 0.48% alpha-terpineol 1191.71 Monoterpenols 21 17.66 0.51% Citronellol 1230.10 Monoterpenols 22 18.01 4.35% Citral 1244.12 Monoterpenes 23 18.23 2.13% Geraniol 1258.29 Monoterpenols 24 18.33 0.16% UK #N/A #N/A 25 18.60 6.78% Geranial 1275.57 Aldehydes 26 19.00 0.54% Bornyl acetate 1288.29 Esters 27 19.19 0.07% Geranyl formate 1304.50 Esters 28 20.14 0.14% Neryl acetate 1362.90 Esters 29 20.73 1.18% Geranyl acetate 1384.71 Esters 30 20.87 0.27% alpha-copaene 1379.00 Sesquiterpenes 31 21.12 0.47% beta-elemene 1388.30 Sesquiterpenes 32 21.28 0.17% 7-epi-Sesquithujene 1398.00 Sesquiterpenes 33 21.75 0.08% beta-caryophyllene 1427.14 Sesquiterpenes 34 21.89 0.29% trans-alpha-bergamotene 1435.00 Sesquiterpenes 35 22.00 0.34% 9-epi-β-Caryophyllene 1449.00 Sesquiterpenes 36 22.07 0.12% beta-cedrene 1450.00 Sesquiterpenes 37 22.14 0.24% (E)-beta-Farnesene 1456.29 Sesquiterpenes 38 22.24 0.29% alpha-humulene 1461.43 Sesquiterpenes 39 22.52 0.18% allo-aromadendrene 1471.00 Sesquiterpenes 40 22.73 12.68% Ar-Curcumene 1482.71 Sesquiterpenes 41 22.85 0.21% beta-selinene 1487.57 Sesquiterpenes 42 22.95 15.92% Zingiberene 1491.75 Sesquiterpenes
5 43 23.07 4.52% (E,E)-alpha-Farnesene 1508.14 Sesquiterpenes 44 23.18 7.41% beta-bisabolene 1509.86 Sesquiterpenes 45 23.47 9.21% beta-sesquiphellandrene 1524.57 Sesquiterpenes 46 23.60 0.25% gamma-bisabolene 1531.75 Sesquiterpenes 47 23.96 0.59% Elemol 1548.57 Sesquiterpenols 48 24.04 0.24% (Z)-Nerolidol 1567.00 Sesquiterpenols 49 24.39 0.14% trans-beta-sesquiphellandrol 1580.00 Sesquiterpenols 50 24.59 0.39% cis-beta-sesquiphellandrol 1586.10 Sesquiterpenols 51 24.98 0.79% Cedrol 1600.04 Sesquiterpenols 52 25.26 0.46% 7-epi-cis-sesquisabinene hydrate 1613.10 Esters 53 25.30 0.32% 10-epi-gamma-Eudesmol 1620.00 Sesquiterpenols 54 25.43 0.23% 7-epi-trans-sesquisabinene hydrate 1631.00 Esters 55 25.56 0.16% Cubenol 1642.14 Sesquiterpenols 56 25.78 0.76% beta-eudesmol 1647.86 Sesquiterpenols 57 25.92 0.18% alpha-eudesmol 1652.86 Sesquiterpenols 58 26.18 0.40% beta-bisabolol 1670.00 Sesquiterpenols 59 26.26 0.15% Uk Sesquiterpene #N/A #N/A 60 26.34 0.31% alpha-bisabolol 1677.50 Sesquiterpenols 61 26.65 0.06% (Z,E)-Farnesol 1709.40 Sesquiterpenols 62 26.86 0.05% Unknown Sesquiterpene #N/A #N/A 63 26.97 0.07% (Z,Z)-Farnesol 1723.00 Sesquiterpenols 64 28.04 0.30% Farnesyl acetate (unknown isomer) 1785.00 Esters