Evaluating Soil Health Using Tea Bags Iowa Watershed Academy October 25, 2017 Marshall McDaniel Teresa Middleton Stefan Gailans
Soil Tilth Soil Quality Soil Health
SOM is central to soil health, but emphasis on soil biology is what separates it from tilth/quality Source: Al-Kaisi and Kwah-Mensah (2016)
The living SOM and soil health
How can we increase soil health? 1. Control traffic on soil 2. Minimum tillage 3. Manure use 4. Cover crops 5. Increase residue cover 6. Crop rotations increase crop variety (i.e. diversity)
How can we measure soil health (specifically the biology)? 1.Many available to scientists 2.Haney Test 3.Solvita 4.Other tests???
Cover crops increase soil biological activity (Solvita) in corn-soybean rotations May 5 Cover No Cover Solvita CO 2 -C (ppm C) 89.1 60.6 24 samples x $50/sample = EXPENSIVE! Cover No cover
Crop rotations increase soil biological activity in extended rotations Percent decomposed: 7 % 20 % #SoilYourUndies 2-year Rotation 4-year Rotation 2-year Rotation 4-year Rotation Source: Matt Woods 7 weeks later
Crop rotations increase soil biological activity in reduced tillage Percent decomposed: 19 % 48 % #SoilYourUndies Conventional Till No-Till Conventional Till No-Till 7 weeks later
The Soil Food Web
Soil Your Undies Works great as a demonstration tool but not as a robust, scientific indicator of biological activity, but It s messy and inconvenient add lots of variability Difficult to compare among soils/treatments Can we develop a scientifically-robust soil health (biology) indictor that is relatively independent of soil temperature and moisture?
How does the health of soil determine decomposition of HIGH and LOW quality residues? Continuous corn Unhealthy Corn-soy-wheat Healthy Could such a comparison be used to calculate a Soil Health Index? McDaniel et al., 2014
How does the health of soil determine decomposition of HIGH and LOW quality residues? Total Respired C (mg CO 2 -C g soil -1 ) 14 12 10 8 6 4 2 Red Clover (13) Unhealthy Healthy Wheat (42) Unhealthy Healthy Baseline (at optimal soil moist & temp) SHI mc = 8.9 / 12.6 = 0.71 SHI CSW2 = 10.7 / 12.8 = 0.84 McDaniel et al., 2014 0 mc CSW2 mc Crop Rotation CSW2 The closer to 1, the more healthy the soil is
SDI increased with crop system complexity Basal Respiration (mg CO 2 -C g soil -1 h -1 ) 40 35 30 25 20 15 mc CS CSW CSW1 CSW2 R 2 = 0.21 P = 0.049 0.7 0.8 0.9 1.0 SDI
It s not easy, or cheap, for just anyone to do this type of experiment!
Use two types of tea bags as easy indicators Rooibos C:N = 43 Green C:N = 12 Relative Mass Remaining (g g -1 ) 1.0 0.8 0.6 0.4 0.2 0.0 Rooibos Tea Green Tea = 60 o F = 80 o F 0 25 50 75 100 125 Time (days) ~$20 for 60 pyramids adapted from Keuskamp et al. (2013)
The Tea Bag Index (or TBI) of Soil Health Unhealthy Soil Healthy Soil Relative Mass Remaining (g g -1 ) 1.0 0.8 0.6 0.4 0.2 0.0 1.0 0.8 0.6 0.4 0.2 0.0 TTTTTT UUUU = TTTTTT HHHH = (11. 00 00. 88) (11. 00 00. 3333) (11. 00 00. 66) (11. 00 00. 3333) = 00. 3333 = 00. 6666 0 25 50 75 100 125 0 25 50 75 100 125 Time (days) = Rooibos Tea Time (days) = Green Tea The closer to 1, the more healthy the soil is
The Soil Food Web
Calibration and Validation of the TBI Microbial Biomass or Activity High Low Healthy Soil Unhealthy Soil Basal Respiration (mg CO 2 -C g soil -1 h -1 ) 40 35 30 25 20 15 mc CS CSW CSW1 CSW2 R 2 = 0.21 P = 0.049 0.2 0.3 0.4 0.5 0.6 TBI SDI 0.7 0.7 0.8 0.9 1.0 SDI
The Approach, 2017 Cover Crop = Healthy Soil, No Cover = Less-Healthy Soil Last summer we buried tea bags (8 cm deep) in 1. Replicated strip trials (n = 3-5) of cover crops of 10 farms located around Iowa wide variety of soil types, fertilizer management, and tillage 2. Nine volunteer farmers buried their own tea bags (not replicated) 3. Retrieved tea bags at 4, 7, 14, 30, 68 and 130 days IOWA Source: http://practicalfarmers.org/blog/2014/04/16/cover-crop-affect-corn-soybean-yields/ N
Step 1: Finding a good row to bury the tea bags Source: http://practicalfarmers.org/blog/2014/04/16/cover-crop-affect-corn-soybean-yields/ a) Row should be between strips, free of wheel traffic and other weird stuff b) Should start a few feet down the row from the field margin c) If you have a GPS, it would be good to mark them or draw a map of where you buried them. Bury them shortly after planting
Step 2: Mark out your holes for the tea bags a) Row of tea bags should parallel crop rows (1/3) distance between rows Rooibos Tea Bag Green Tea Bag
Step 3: Dig small holes for each tea bag a) With a hand towel dig a small hole for each tea bag (~2.4 wide, 3.2 deep) b) Place the teabag inside, and cover with soil c) Gently pat down with tag remaining on surface STEP 3a STEP 3b and 3c
Step 4: Retrieving buried tea bags a) Collect on the date suggested, or within the acceptable range b) Record the date, weather, any other information on the conditions (mail and email information to ISU) c) Gently pull (or excavate) two tea bags (green and rooibos) out of the soil from each plot d) Carefully brush off any soil adhering to the tea bags Order Tea Bag Time After Burial Letters (acceptable range of days) 1 a 4 (3-5) 2 b 7 (6-8) 3 c 14 (13-15) 4 d 30 (28-32) 5 e 68 (64-72) 6 f 130 (125-135) Date Retrieved
Step 5: Handling the tea bags after retrieval a) The tea bags should be dried at about 140 o F, but most ovens do not go this low and are not consistent at this low temperature
The Approach, 2017 Cover Crop = Healthy Soil, No Cover = Less-Healthy Soil Last summer we buried tea bags (8 cm deep) in 1. Replicated strip trials (n = 3-5) of cover crops of 10 farms located around Iowa wide variety of soil types, fertilizer management, and tillage 2. Nine volunteer farmers buried their own tea bags (not replicated) 3. Retrieved tea bags at 4, 7, 14, 30, 68 and 130 days IOWA Source: http://practicalfarmers.org/blog/2014/04/16/cover-crop-affect-corn-soybean-yields/ N
Hypothesis: SDI with tea bags to measure Soil Health Unhealthy Soil Healthy Soil Relative Mass Remaining (g g -1 ) 1.0 0.8 0.6 0.4 0.2 0.0 1.0 0.8 0.6 0.4 0.2 0.0 SSSSSS UUUU = SSSSSS HHHH = (11. 00 00. 6666) (11. 00 00. 3333) (11. 00 00. 4444) (11. 00 00. 3333) = 00. 3333 = 00. 6666 0 25 50 75 100 125 0 25 50 75 100 125 Time (days) = Rooibos Tea Time (days) = Green Tea The closer to 1, the more healthy the soil is
Not much difference between COVER and NO COVER crop, yet 100 80 Farm A Farm B Farm C Farm D Farm E 60 % Mass Remaining 40 20 100 80 60 Farm F Farm G Farm H Farm I 0 20 40 60 80 100 120 No Cover_Green Tea Cover_Green Tea No Cover_Red Tea Cover_Red Tea 40 20 0 20 40 60 80 100 120 0 20 40 60 80 100 120 0 20 40 60 80 Day 100 120 0 20 40 60 80 100 120
7/9 farms have higher SDI with cover crops, but a lot of variability Soil Decomposition Index 0.6 0.5 0.4 0.3 0.2 Cover Crop No Cover A B C D E F G H I Ave. Farm ** Difference in SDI (CC - No Cover) 0.25 0.20 0.15 0.10 0.05 0.00-0.05-0.10 A B C D E F G H I Ave. * = P < 0.1 Farm * * ** = P < 0.05
The SDI with Tea Bags Shows Some Promise, Next Steps What soil and management factors explain the variation in SDI? Look at other aspects of decomposition as an index (i.e. rate) Validate any TB index with known measures of soil biological health Look at microbial community composition in 7 d and 130 d tea bags Sociological survey on participants (citizen scientists) and IA farming community Expand to other long-term experiments in Iowa and beyond!?!?
Materials Needed Provided by PFI and ISU, or in the Tea Bag Kit 48 green and rooibos tea bags 48 Ziploc snack plastic bags 6 Pre-addressed envelope mailers 8 flags for marking plots You will need to provide Hand trowel (depending on soil) Bucket or Rubbermaid tote to carry Ziploc bags and collect teas Notebook, pen, and sharpie to record any unusual findings/observations
Procedure Overview 1. Finding a good row to place the tea bags 2. Mark out your holes for the tea bags 3. Dig small holes for each tea bag 4. Retrieving buried tea bags 5. Handling the tea bags after retrieval 6. Sending the tea bags to ISU
Step 6: Sending the tea bags to ISU a) No more than a day or two after you collected and dried the tea bags b) Close the Ziploc bags c) Place all of the Ziploc bags (each with 2 tea bags) into a pre-addressed mailer provided by PFI d) Drop it off at the post office e) You are ready for the next collection date! Marshall McDaniel 2517 Agronomy Hall Ames, IA 50011
Incorporating landscape position helps to eliminate some noise Soil Decomposition Index 0.6 0.5 0.4 0.3 0.2 Cover Crop No Cover A B C D E F G H I Ave. Farm ** Difference in SDI (CC - No Cover) 0.25 0.20 0.15 0.10 0.05 0.00-0.05-0.10 * = P < 0.1 ** * ** * A B C D E F G H I Ave. Farm ** = P < 0.05