The 11 th TSAE International Conference April 2018

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The 11 th Thai Society of Agricultural Engineering International Co, 26-27 April 2018 The 11 th TSAE International Co 26-27 April 2018 Available online at www.tsae.asia Characteristic and Some Physical Properties of Dried Ripe Fruit of Sacha Inchi (Plukenetia volubilis L.) Co Nuttaphon Sokudlor 1,2,3 *, Kittipong Laloon 1,2,3, Sakda chumpana 2,3, Somposh Sudajan 1,2,3 1 Department of Agricultural Engineering, Faculty of Engineering, Khon Kaen University, Khon Kaen, 40002, Thailand 2 Postharvest Technology Innovation Center, Commission on Higher Education, Bangkok 10400, Thailand 3 Agricultural Machinery and Postharvest Technology Center, Khon Kaen University, Khon Kaen, 40002, Thailand *Corresponding author: Tel: +66-8-6853-3832, Fax: +66-43-362-149, E-mail: sokudlor@kkumail.com Abstract The physical properties of agricultural products are very useful in processing improvement such as harvesting, transportation and storage. In this study, physical properties of sacha inchi is important in designing machinery to preparation of processing chain from raw materials to product. Dried ripe fruit of sacha inchi from Lampang province (Northern part of Thailand) with moisture content of 4.05% w.b. was studied. The fruit was composed of skin (Exocarp Part) 19.32%, shell (Mesocarp Part) 26.84%, seed soat (Endocarp Part) 18.73 % and kernel (Endosperm) 34.85%. The fruit s diameter was in the range of 35.17 to 50.95 mm. Thickness was in the range of 22.82 to 24.47 mm. Breadth of pod and seed were 23.38 to 25.45 mm. and 15.67 to 17.68 mm. Length of pod and seed were 21.15 to 25.20 mm. and 18.85 to 21.43 mm. Thickness of pod and seed were 14.54 to 15.61 mm. and 8.34 to 8.80 mm. Angle of repose of fruit, pod and seed were 41.0 to 52.5 degrees 23.2 to 27.0 degrees and 27.2 to 31.3 degrees, respectively. Static coefficient of friction on steel /stainless /rubber were fruit: 0.781 to 0.869/ 0.726 to 0.839/ 0.839 to 0.932 pod: 0.754 to 0.810/ 0.781 to 0.839/ 0.839 to 0.932 and seed: 0.600 to 0.753/ 0.488 to 0.554/ 0.649 to 0.753 respectively. Bulk density of fruit pod and seed were 655.0 725.5 and 999.4 kg/m 3 respectively. Co Keywords: Sacha Inchi, Physical Properties, Fruit composition of Sacha Inchi applications in the food and pharmaceutical industries 1 Introduction and Gustavo F. et al. (2014) have reported sacha inchi Native to the Amezon jungles plant, sacha inchi oil consumed has good acceptability after week-1 of (Plukenetia volubilis L.), also known as the Inca consumption and it is safety. Most Informations and peanut. Plant of the Euphorbiaceae family alike Cassava (Manihot esculenta), castor plant (Ricinus communis) and jatropha (genus Jatropha). Sacha inchi is a potential oilseed crop because the seeds of this plant are rich in oil and proteins (35 60% oil and Co around 27-33% proteins) (M.D. Guillén et al., 2003; A.K.L. Nascimento et al., 2013), The oil is characterized by its high content of unsaturated fatty acids (FAs), particularly A-Linolenic Acid (ALA), a kind of omega-3 fatty acids and in less proportion Linoleic Acid (LA), a kind of omega-6 fatty acids (Gustavo F et al., 2014). Many researches have reported the benefits of sacha inchi oil (SIO). Such Longjian Niu et al. (2014) have reported sacha inchi oil has agreat potential for applications in the food and pharmaceutical industries according Luis-Felipe Gutiérrez et al. (2011) have reported sacha inchi is an important new crop with 164 research supported the performance of SIO indicated this crop can be apply to commercial production. However, there is very little information on the physical properties of sacha inchi dried fruits or seeds. The physical properties very important for processing chain, which affects to quantity and quality from raw materials to product. The objective of this study was to determine characteristic and some physical properties of dried ripe fruit of sacha inchi, so that the knowledge gained Co will be used in optimizing machine design parameters. 2 Materials and Methods 2.1 Raw material and sample preparation Dried ripe fruit of sacha inchi used in this study were procured from north parts (Lampang province: 8 20'54.2"N 99 24'31.6"E) of Thailand. The sample Co

The 11 th Thai Society of Agricultural Engineering International Co, 26-27 April 2018 was cleaned manually to remove all foreign materials. The initial moisture content was determined by drying samples in a hot air oven set at 105 C (±1 C) for 24 h (ASAE, 1994) and was found to be 4.05% w.b. Five sample were selected from a total bulk of 200 kg, each sample weighted with an electronic balance with an accuracy of ±0.001g then separated into classes according of pods. Counted and weighed each classes, it was found that can be divided into four groups include 4 5 6 and 7 pods, each group has proportion by were 45.81% 37.06% 14.94% and 2.19% respectively, and proportion by weight were 42.5% 38.2% 17.9% and 1.4% respectively. Because proportion of 7 pods are very small compared to other groups, and not enough for study. The preparation of sample for this study were divided into three main groups were characterized by 4 pods, 5 pods and 6 pods. After the separation process, samples were were kept in an airtight plastic bags and stored at 5 C until use. Before starting a test the samples were allowed to warm up under ambient room conditions (22 25 C, 30 40% RH) to the equilibrium moisture (V. Sharma et al., 2011). 2.2 Structure and composition of fruit Randomly three sample from three main groups (30 fruits per sample), weighted with an electronic balance. Then, separate and weighted the fruit components according fruit structurals as shown in Fig. 1 include 4 parts were skin ( Exocarp) husk (Mesocarp) seed coat (Endocarp) and kernel ( Endosperm) ( Cappers & Bekker, 2013), and computed percentage by weight. Figure 1 Fruit structural of Sacha Inchi Dried Ripe Fruit. 2.3 Physical properties of Plukenetia volubilis L. 2.3.1. Size of samples Ninety samples were selected from three main groups. To measuring the average size of the fruits, the diamentions (D) and thickness (T) were measured using a digital caliper with an accuracy of 0.01 mm. the measurement as shown in Fig. 2, then splitting the pods out of the fruit and measuring the pods size in terms of length (x) breadth (y) and thickness (z) (S. Karaj and J. Müller, 2010). After that, shelled the pods manually and measured the seeds size same the pods methods, the measurement of pods and seeds size as shown in Fig. 2. Co Co 165 Fruit Pod Seed Figure 2 Size measurement of Fruits Pods and Seeds. 2.3.2 Shape of sacha Inchi. The shape of Sacha inchi fruits, Chiara Fanali et al. (2011) are reported that shaped as a star-shaped. For the shape of pods and seeds determined in terms of arithmetic mean diameter geometric mean diameter and sphericity the procedure of measurement as follow: The arithmetic mean diameter (D a ) and geometric mean diameter (D g ) of samples were calculated from the geometrical dimensions as (Mohsenin, 1980; S. Karaj and J. Müller, 2010): D a = (x+y+z) (1) 3 and Co D g =(x y z) 1/3 (2) Sphericity (Ø) of samples was calculated based on the isoperimetric property of a sphere (Mohsenin, 1980): Co Ø = (x y z)1/3 x = D g x (3) The higher the sphericity value nearly one denotes its shape closer to a sphere. Co

The 11 th Thai Society of Agricultural Engineering International Co, 26-27 April 2018 2.3.2 Bulk density, Angle of repose and Coefficient of static friction The bulk density was measured by weighing a filled measuring cylinder with known volume and calculated as (S. Karaj and J. Müller, 2010): ρ b = m v (4) where ρ b is bulk density (g/cm 3 ), v is container volume (m 3 ) and m is mass (g) of the sample. The angle of repose ( ) is the angle of repose is the angle from the horizontal at which the material will rest in a pile. This was determined by using an open-ended cylinder. The cylinder was placed at the centre of a plate and was filled with samples. The samples was released slowly from cylinder until it formed a cone on the plate. Measured the height (H) and the base diameter of cone. The angle of repose was calculated using the formula (R.C. Pradhan et al., 2009): = tan -1 [ 2H D ] (5) The static coefficient of friction (µ) was determined on three different materials namely steel stainless and rubber. The procedure of measurment was conducted to the methodology described by R.C. Pradhan et al. (2009) and V. Sharma et al. (2011). The static coefficient of friction was calculated using the formula: μ = tan α (9) where µ is the coefficient of friction and α is the angle of tilt in degrees. 3 Results and Discussion The initial moisture content of sample was 4.05% w.b. The results of this study are presented as follows: 3.1 Structure and composition of fruit Sacha Inchi fruit was composed of skin (Exocarp Part) shell (Mesocarp Part) seed coat (Endocarp Part) and kernel (Endosperm). In 4 pods composed of skin shell seed coat and kernel were 19.46 26.95 18.38 and 33.98 % by weight respectively. In 5 pods were 19.75 26.73 18.73 and 34.13 % by weight respectively and in 6 pods was 18.71 26.85 19.08 and 36.45 % by weight respectively. The result presented in table 1 166 Table 1 Result of Sacha Inchi fruits composition. Number of pods 4 5 6 % Composition by weight Skin Shell Seed Coat Kernel Max 22.02 33.33 20.69 39.02 Min 16.95 24.39 15.38 26.26 Mean 19.46 26.95 18.38 33.98 Max 23.41 31.73 21.74 39.22 Co Min 16.09 20.83 18.52 22.12 Mean 19.75 26.73 18.73 34.13 Max 21.33 31.97 21.21 38.68 Min 16.09 20.45 15.38 22.12 Mean 18.71 26.85 19.08 36.45 3.2 Physical properties of Plukenetia volubilis L. 3.2.1 The size of sacha inchi. The size characteristics of sacha inchi fruits in terms of length, breadth and thickness presented in Table 2. It was observed that fruit s diameter was in the range of 35.17 to 50.95 mm. Thickness was in the range of 22.82 to 24.47 mm. Breadth of pod and seed were 23.38 to 25.45 mm. and 15.67 to 17.68 mm. Length of pod and seed were 21.15 to 25.20 mm. and 18.85 to 21.43 mm. Thickness of pod and seed were 14.54 to 15.61 mm. and 8.34 to 8.80 mm. Table 2 Basic dimensions characteristics of sacha inchi. Properties Max Min Mean SD Fruits Diameter (mm) 50.95 35.17 42.95 2.46 Thickness (mm) 24.47 22.82 23.69 0.93 Pods Breadth (mm) 25.45 23.38 24.01 2.51 Length (mm) 25.20 21.15 23.15 2.01 Thickness (mm) 15.61 14.54 15.19 0.76 Seed Breadth (mm) 17.68 15.67 16.52 1.10 Length (mm) 21.43 18.85 19.74 1.20 Thickness (mm) 8.80 7.67 8.27 1.08 Co Co 3.2.2 The shape of sacha Inchi pods and seeds The shape of pods and seeds was presented in table 3. It was observed that sphericity of pods higher mean values compared with seeds. 3.2.3 Bulk density, Angle of repose and Coefficient of static friction Bulk density are presented in table 4 demonstrates since seeds size was smaller than pods and fruits size, more unit of seeds were entering into the container volume compared with unit of pods and fruits. The seeds fraction was highest values of bulk density that Co Co

The 11 th Thai Society of Agricultural Engineering International Co, 26-27 April 2018 was in the range of 998.8 to 999.9 kg/m 3, fraction of pods was in the range of 717.7 to 729.2 kg/m 3 and fruits fraction was in the range of 650.7 to 660.0 kg/m 3. Angle of repose of fruits pods and seeds was presented in table 4. The highest values of angle of repose were recorded from fraction of fruits with in the range 41.0 to 52.5 degrees. It was also noticed that angle of repose of seeds that was higher than for pods. Similar results were obtained by Shkelqim Karaj and Joachim Müller (2010). This might be explained by the cohesion forces between seeds units are stronger than cohesion between pods units. Coefficient of static friction of fruits on various surfaces showed that static friction on rubber is higher then on other surfaces and static friction on stainless is the lowest. Coefficient of static friction of pods similarly fruits was highest on ruber but lowest on steel. Coefficient of static friction of seeds on stainless and rubber surfaces are equally but lowest on steel. The coefficient of static friction of fruits was highest on all surfaces. This might be explained by the friction directed variation with weight. The fruits are heavier combined with roughness skin's surface, which not allowed moving easily on the studied surfaces. Table 3 Geometric characteristics of Sacha inchi pods and seeds (mean values±standard deviation). Arithmetic Geometric Sphericity Sample diameter diameter Ø source D a (mm) D g (mm) Pods 4 pods 20.29±0.49 19.95±0.44 0.944±0.031 5 pods 20.74±1.02 20.21±0.99 0.832±0.028 6 pods 22.03±1.56 21.45±1.36 0.853±0.030 Seeds 4 pods 15.12±0.53 14.24±0.47 0.710±0.009 5 pods 14.48±1.47 14.07±1.58 0.798±0.798 6 pods 15.62±1.32 14.31±1.08 0.669±0.020 Co Co Table 4 Complex characteristics of Sacha inchi. Properties Fruit Pod Seed Max Min mean Max Min mean Max Min mean Bulk density (kg/m 3 ) 660.0 650.7 655.1 729.2 717.7 725.5 999.9 998.8 999.4 Angle of repose ( ๐ ) 52.5 41.0 46.0 27.0 21.9 24.0 31.3 27.2 29.4 Static coefficient of friction (μ) - steel 0.900 0.759 0.824 0.787 0.776 0.783 0.577 0.554 0.566 - stainless 0.810 0.708 0.761 0.816 0.770 0.799 0.695 0.669 0.685 - rubber 0.907 0.869 0.887 0.913 0.827 0.851 0.695 0.669 0.685 Co 4 Conclusions The component of Plukenetia volubilis L. dried ripe fruits only 32% can used for the processing of foods in greated values product, orther mass are shell, skin and seed coat more than 60% have to be investigated in further research for value added. Size characteristics of sacha inchi fruits in terms of geometical diamentions. Average fruit diameter was 42.95 mm and 23.69 mm with thickness. The average diamentions of pod, length breadth and thickness were 23.15 24.01 and 15.19 mm respectively, for the seeds were 19.74 16.52 and 8.27mm respectively. Shape of Sacha inchi fruits shaped like star-shape. When considered in sphericity for determining the shape of pods and seeds, the pods fraction nearly a shape of sphere than seeds. The complex properties determining in term of bulk density, angle of repose and coefficient of static friction, it could be used for sorting or designing or designing another process for further. 5 Acknowledgements The authors are thankful to The Agricultural Machinery and Postharvest Technology Center, Khon Kaen University and Postharvest Technology Innovation Center, Commission on Higher Education, Bangkok, for provided supporting and all of facilitated in this studied. 6 References ASAE, 1994. S352.3. Moisture measurement ungrounded grains and seeds. Ana Karina Lima Nascimento, Raniere FagundesMelo-Silveira, Nednaldo Dantas-Santos, JúliaMorais Fernandes, SilvanaMaria Zucolotto, Hugo Alexandre Oliveira Rocha and Katia Castanho Scortecci. 2013. Antioxidant and Antiproliferative Activities of Leaf Extracts from Plukenetia volubilis Linneo (Euphorbiaceae). Co 167 Co

The 11 th Thai Society of Agricultural Engineering International Co, 26-27 April 2018 Evidence-Based Complementary and Alternative Medicine. Volume 2013 Chiara Fanali, Laura Dugo, Francesco Cacciola, Marco Beccaria, Simone Grasso, Marina Dacha, Paola Dugo and Luigi Mondello. 2011. Chemical Characterization of Sacha Inchi (Plukenetia volubilis L.) Oil. Journal of Agricultural and Food Chemistry. 59, 13043 13049 Cappers, R & Bekker, R 2013, A manual for the identification of plant seeds and fruits. Groningen Archaeological Series, vol. 23, Barkhuis Publishing & Groningen University Library. Gustavo F. Gonzales and Carla Gonzales. 2014. A randomized, double-blind placebo-controlled study on acceptability, safety and efficacy of oral administration of sacha inchi oil (Plukenetia volubilis L.) in adult human subjects. Food and Chemical Toxicology. 65 (2014) 168 176 Longjian Nui, Jialong Li, Mao-Sheng Chen, Zeng-Fu Xu. 2014. Determination of oil contents in Sacha Inchi (Phukenetia Volubilis) seed at different developmental stages by two method: Soxhlet extraction and Time-domain nuclear magnetic resonance. Industrial Crops and Products. 56: 187-190. Luis-Felipe Gutiérrez, Lina-María Rosada and Álvaro Jiménez. 2011. Chemical composition of 168 Sacha Inchi (Plukenetia volubilis L.) seeds and characteristics of their lipid fraction. 77 grasasy aceites, 62 (1), enero-marzo, 76-83. Mohsenin, N.N., Physical properties of plant and agricultural materials. Gordon and Breach Science Publishers, New York. 1980. María D. Guillén, Ainhoa Ruiz, Nerea Cabo, Rosana Chirinos, and Gloria Pascual. 2003. Characterization of Sacha Inchi (Plukenetia volubilis L.) Oilby FTIR Spectroscopy and 1H Co NMR. Comparison with Linseed Oil. Journal of the American oil chemists' society (JAOCS), Vol. 80, no. 8 (2003) R.C. Pradhan, S.N. Naik, N. Bhatnagar and V.K. Vijay. Moisture-dependent physical properties of jatropha fruit. Industrial crops and products. 9 (2 0 0 9) 341 347 Shkelqim Karaj, Joachim Müller. 2010. Determination of physical, mechanical and chemical properties of seeds and kernels of Jatropha curcas L.. Industrial Crops and Products. 32 (2010) 129 138 Sharma V, Das L, Pradhan R, Naik S, Bhatnagar N, Co Kureel R. 2011. Physical properties of Tung seed: An industrial oil yielding crop. Industrial Crops and Products. 33(2) 440-444 Co Co Co