Capacity Building in Veterinary Public Health and Livestock Biotechnology: (VPH-BIOTECH GLOBAL)

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1 Capacity Building in Veterinary Public Health and Livestock Biotechnology: (VPHBIOTECH GLOBAL) WOWOSSEN A. GEBREYES THE OHIO STATE UNIVERSITY

2 Infectious Diseases Molecular Epidemiology Laboratory (IDMEL): Foodborne Pathogens Salmonella Campylobacter Yersinia Nosocomial pathogens Staphylococcus Clostridium Acinetobacter Understanding the Molecular Mechanism of Antimicrobial Resistance and hypervirulence Farm/ Hospital bed to Genomes

3 Goals Strengthening/building a sustainable capacity for an integrated food safety, zoonoses, veterinary public health and Livestock biotechnology using the One Health model. Why zoonotic diseases? 75% of emerging infections Why One Health model? Prevention of control needs integrated partnership among disciplines.

4 Current partners! VPH Biotech Global Consortium

5 Formats for Capacity Building 1. Institutional Training Modular skill building courses, 2. Technology transfer Genotyping etc. 3. Graduate fellowship and innovation: MSc and PhD levels 4. Knowledgesharing and awareness expanding skills and modifying behavior 5. Metaleadership skill development effective interaction among stakeholders

6 Examples of VPHBiotech Global activities Skill building courses: Molecular Epidemiology of Infectious Diseases Milk Quality and genotyping projects Collaborators: OSU, UFPB, UNIVASF, and INSA Sponsor: OSU Global Gateways Grant Int l Congress on pathogens at the Human Animal Interface (ICOPHAI)

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8 MICROBIOLOGICAL QUALITY OF MILK A GENOTYPING IN SEMIARID BRAZIL

9 Federal University of Paraiba College for Agricultural Sciences Department of Animal Sciences Genotypic relatedness of staphylococci isolated from critical control points of smallscale dairy plants for goat milk pasteurization in semiarid Brazil Celso Oliveira 1, Francisca Sousa 1, Melanie Abley 2, Ariana Meira 1, Wondwossen Gebreyes 2 1 Center for Agricultural Science, Federal University of Paraiba (CCA/UFPB), Brazil 2 College of Veterinary Medicine, The Ohio State University (OSU), USA ICOPHAI 2011

10 Goats and NORTHEAST BRAZIL 95% of goats concentrated in developing countries Arid and semiarid regions 93% of the Brazilian goat herd/ NE Brazil 67% of all goat milk production Highest rural concentration of impoverished people in Latin America 67% of population living below the poverty line Thus limited ability for professional oversight

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12 BACTERIAL CONTAMINANTS OF MILK: Milk: excellent medium for pathogen growth. Brucella Campylobacter Listeria Salmonella Staphylococcus Tuberculosis E. coli Shigella Streptococcus pyogenes Yersinia enterocolitica All are zoonotic (infect humans and animals.) Milk adulteration and Fome Zero

13 Governmental Programs Private Market MILK QUALITY A SAFETY ICOPHAI 2011

14 Key findings: Less than 40% of the goat milk samples complied with the national regulations for raw goat milk bacterial threshold. High prevalence and counts of staphylococci in milk Higher somatic cell count in milk as compared to other countries. Range from 275,860 cells/ml to 11,055,620 cells/ml Further investigations: Qualitative impacts of public health: Antimicrobial resistance Genotypic analysis to determine dissemination patterns.

15 STAPHYLOCOCCUS SPP. Staphylococcus spp 100% prevalence Range from 500 cfu/ml 30,900,000 cfu/ml Mean value= 1,971,206 cfu/ml

16 ATCC M c N CÓDIGO IDENTIFICAÇÃO ATCC ST 0016/08 Faz. 7 2 ST 0020/08 Faz. 8 3 ST 0040/08 Faz ST 0056/08 Faz ST 0086/08 Faz ST 0087/08 Faz ST 0088/08 Faz ST 0095/08 Faz ST 0096/08 Faz ST 0101/08 Faz. 28 N CÓDIGO IDENTIFICAÇÃO 11 ST 0102/08 Faz ST 0103/08 Faz ST 0104/08 Faz ST 0110/08 Faz ST 0111/08 Faz ST 0112/08 Faz ST 0124/08 Faz ST 0131/08 Faz ST 0132/08 Faz ST 0140/08 Faz ST 0141/08 Faz. 35

17 (82 entries) Pearson correlation (Opt:1.00%) [0.0%100.0%] Rep PCR Rep PCR NM3b CC8 77 PFGE and MLST Genotyping P6S S aureus a ATCC CPC02b Cabaceiras Pasteurized milk 55.1 CPC02a Cabaceiras Pasteurized 90.8 Key Dairy Plant Source Coagulase P7S9a Sumé Hand swab from dairy plant worker PM10b Monteiro Pasteurized milk P7S9b Sumé Hand swab from dairy plant worker 84.5 CM36a Monteiro Raw milk CM36b Monteiro Raw milk P2M3a Monteiro Equipment of dairy plant 98.1 CC2b Cabaceiras Raw milk 70 CM35 Monteiro Raw milk 49 P2M3b Monteiro Equipment of dairy plant P2S9a Sumé Equipment of dairy plant P2S9b Sumé Equipment CCC02 Cabaceiras Raw milk CCC02a Cabaceiras Raw milk PM10a Monteiro Pasteurized milk CC6 Cabaceiras Raw milk NM11 Monteiro Raw milk CC10 Cabaceiras Raw milk 79.9 CM29 Monteiro Raw milk PS4 Sumé Pasteurized milk NS9a Sumé Raw milk CS29 Sumé Raw milk 72.9 P6S4b 47.8 CS23 Sumé Raw milk CC1 Cabaceiras Raw milk CM30 Monteiro Raw milk 96.9 PS03a Sumé Pasteurized PS03c Sumé Pasteurized CC2a Cabaceiras Raw milk S. aureus c ATCC 2593 S. aureus b ATCC PM12 Monteiro Pasteurized NS9b Sumé Raw milk 89.7 PS03b Sumé Pasteurized P3S10a Sumé Equipment of dairy plant 83 PS08 Sumé Pasteurized P6S4a Sumé Equipment of dairy plant NM11a Monteiro Raw milk NM11b Monteiro Raw milk NS9c Sumé Raw milk PM11a Monteiro Pasteurized CS22 Sumé Raw milk P6S2 Sumé Hand of worker of dairy plant NS2 Sumé Raw milk. PM11b 97.6 P3S10b NM3a Monteiro Raw milk P6M3b Monteiro Hand of worker of dairy plant NM3b Monteiro Raw milk. CC8 cabaceiras Raw milk 88.7 P8S2 Sumé Hand of worker of dairy plant P6S7 Sumé Hand of worker of dairy plant S. aureus d ATCC S aureus a ATCC P6M3a Monteiro Hand of worker of dairy plant CPC02b Cabaceiras Pasteurized milk CPC02a Cabaceiras Pasteurized PM10b Monteiro Pasteurized milk CCC03a Cabaceiras Raw milk P2M3a Monteiro Equipment of dairy plant P2M3b Monteiro Equipment of dairy plant CCC03b Cabaceiras Raw milk. CCC02a Cabaceiras Raw milk CC10 Cabaceiras Raw milk NS9a Sumé Raw milk 41.3 NM12b Monteiro Raw milk CC1 Cabaceiras Raw milk CC2a Cabaceiras Raw milk NS9b Sumé Raw milk CCM04c Monteiro Raw milk PS08 Sumé Pasteurized 98.5 NS9c Sumé Raw milk 75.6 NS2 Sumé Raw milk CPM04b Monteiro Pasteurized P6M3b Monteiro Hand of worker of dairy plant P8S2 Sumé Hand of worker of dairy plant 85.7 CPM04a Monteiro Pasteurized P6M3a Monteiro Hand of worker of dairy plant CCC03a Cabaceiras Raw milk CCC03b Cabaceiras Raw milk. PS9 Sumé Pasteurized milk NM12b Monteiro Raw milk CCM04c Monteiro Raw milk CPM04b Monteiro Pasteurized 80.8 NM10 Monteiro Raw milk CPM04a Monteiro Pasteurized 98 PS9 Sumé Pasteurized milk 72.3 NM10 Monteiro Raw milk 92.5 NS8a Sumé Raw milk NS8a Sumé Raw milk NS4a Sumé Raw milk 87.6 NS4a Sumé Raw milk NS4b Sumé Raw milk NS03a Sumé Raw milk NS03c Sumé Raw milk NS4b Sumé Raw milk NS03b Sumé Raw milk NS8b Sumé Raw milk NS8c Sumé Raw milk 56.6 NS03a Sumé Raw milk NM12a Monteiro Raw milk 99.5 P6M11a Monteiro Hand of worker of dairy plant P6M11b Monteiro Hand of worker of dairy plant 98.4 NS03c Sumé Raw milk P8M3a Monteiro Hand of worker of dairy plant CPC03a Cabaceiras Pasteurized milk NS03b Sumé Raw milk CPC03b Cabaceiras Pasteurized milk 48.8 P8M3b Monteiro Hand of worker of dairy plant CC7 Cabaceiras Raw milk NS8b Sumé Raw milk P8S4 Sumé Pasteurized milk 79.2 Unique MLST= ST313 was common P3S8 Sumé Equipment NM3a S. aureus d NS8c Monteiro Monteiro cabaceiras Sumé ATCC Sumé Raw milk. Raw milk. Raw milk Hand of worker of dairy plant Raw milk NM12a Monteiro Raw milk P6M11a Monteiro Hand of worker of dairy plant P6M11b Monteiro Hand of worker of dairy plant

18 MILKMILK ADULTERATION G B TWO BAS= Goat milk adulterated with bovine milk. G= Only goat milk B= Only bovine milk Test limit of detection= 0.5% (Lane 3).

19 Methiciilin Resistance/ MRSA Common in milk in NE Brazil and similar genotypes with that of milk from the U.S. Dice (Tol 1.0%1.0%) (H>0.0% S>0.0%) [0.0%100.0%] Brazil Staph Aureus Brazil Staph Aureus Key Origin Host SRpattern meca blaz PBP2' SCCmec ST00104 ST00108 USA USA Feline Canine. AmAnAxChEnErOxMrPbPn. AmAnAxChEnErLnOxMrPbPn I & II I & II 35.4 ST00094 USA Equine. AmAxChGmOxPbPnTbTr IV ST00075 USA Equine. AmAxChGmOxPbPnTr IV ST00877 ST00878 ST00293 ST00296 USA USA Brazil Brazil Bovine Bovine Bovine Bovine. AmOxPbPn. AmOxPbPn. OxPbPn. AmCfOxPbPn I & IV I & IV ST00356 ST00357 Brazil Brazil Bovine Bovine. Ox. Ox No No I & II I & II ST00266 Brazil Bovine. AmAxOxPn None ST00284 ST00313 Brazil Brazil Bovine Bovine. AmAxOxPbPnTe. ChOxPbPnTe No I & III I 77.7 ST00304 Brazil Bovine. AmOxPbPnTe I & III ST00325 Brazil Bovine. AmOxPn I & III ST00375 Brazil Bovine. AmOxPbPnTe I

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21 Organizing partners VPH Biotech Global Consortium

22 8 key thematic areas 3 keynote and 8 plenary speakers 45 oral presentations 93 poster presentations 275 Participants from 34 nations 356 online registrants: following via Video streaming 147 participants received scholarship Full, partial or registration fee sponsorship

23 Closing remarks THEMATIC AREAS Enteric diseases, food and waterborne pathogens Wildlife and interface at human diseases Respiratory diseases and global impact Drug discovery and antimicrobial resistance Parasites and environmental impact Genomics and molecular epidemiology Immunology and vaccine development Policy, capacity building and other significant issues All slides and videos on

24 Sponsors

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26 ICOPHAI 2013 Brazil Interested to partner/ listserve/ sponsor: or

27 Muito Obrigado! Asante Sana! Muchas gracias! በበበበበበበበበበበበበ Merci Beaucoup!