Volatile monophenols in belgian special beers : identification of specific markers
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1 Volatile monophenols in belgian special beers : identification of specific markers C. Scholtes, M. Shiba, T. Haube, S. Nizet and S. Collin Caroline Scholtes PhD student UCLouvain Brewing department
2 Volatile monophenols in belgian special beers 1. State of the art
3 Volatile phenols Important flavor contributors in beverages e.g : Weizen beers and Belgian white beers
4 associated with lignins (ether or ester bound) or under hydroxycinnamic acids form (Li et al., 2008) Phenylpropanoid pathway (stress related) L-phenylalanine monophenols Lignin-carbohydrate complex in herbaceous straws (Buranov et al., 2008) Lignin polymer (Gross et al., 2007) Glycoside forms
5 Hydroxycinna mic acids Structure Malt (ppm) Concentration in Hop (ppm) Pils (ppm) Threshold in beer (ppm) Ferulic acid 7,8-12,8 13,2-14,1 5,6-13,1 > 660 p-coumaric acid 0,3-1,3 2,2-2,8 0,21-1,45 Sinapic acid ,4-4,5 - (Wackerbauer and Kramer, 1982) 520
6 Little impact of hydroxycinnamic acids on beer profile! Structure Malt (ppm) Concentration in Hop (ppm) Pils (ppm) Threshold in beer (ppm) Ferulic acid 7,8-12,8 13,2-14,1 5,6-13,1 > 660 p-coumaric acid 0,3-1,3 2,2-2,8 0,21-1,45 Sinapic acid ,4-4,5 - (Wackerbauer and Kramer, 1982) 520
7 Hydroxycinnamic acids decarboxylation Phenylacrylic acid decarboxylase (PF + yeast) Saccharomyces cerevisiae Brettanomyces/Dekkera spp. ou Enterobacteriacea. (McMurrough et al., 1996, Suarez et al., 2007 ) Volatile phenols 7
8 Malting Thermic decarboxylation Brewing T Humidity T Humidity Lignin pyrolysis
9 H p-coumaric acid C 2 4-vinylphenol 2 p-hydroxybenzaldehyde H C 2 Ferulic acid 4-vinylguaiacol vanillin 2 H 2 H p-hydroxybenzaldehyde apocynol C 4-methylphenol C 4-methylguaiacol 2 Phenol guaiacol Me H Me sinapic acid Me Me syringaldehyde acetovanillon H H o-coumaric acid salicylaldehyde
10 Volatile monophenols in belgian special beers : 3. Material & methods
11 BL1 BL2 BL3 BL4 Blond AM1 Amber Conventional Triple rganic Blond Amber Trappist Brown TR1 BR1 7 Commercial Belgian special beers Dry-hopped Trappist Brown XIV Jean De Clerck chair September 2012
12 5 EBC 50 EBC Green malt kilning 15 min 125 C 500 EBC 10 min 180 C 900 EBC 1500 EBC PILSEN MALT TRREFACTR step : until 225 C 10 min 225 C 20 min 225 C
13 EBC method congress wort Malt EBC Congress wort Phenols Ebullition
14 echerchés 4-Vinylguaiacol 4-Methylphenol 4-Ethylphenol 4-Vinylphenol Vanillin Guaiacol 4-Methylguaiacol 4-Ethylguaiacol Apocynol
15 GC-MS analysis (SIM Mode) 4-Methylphenol Guaiacol 4-Ethylphenol 4-Vinylphenol 4-Ethylguaiacol 4-Vinylguaiacol Vanillin Apocynol 4-Methylguaiacol
16 Volatile monophenols in belgian special beers : 4. Results
17 A. Analysis of fresh beers
18 Concentration (µg/l) Concentration (µg/l) 2,5 2 1,5 1 0,5 0 BL3 BL4 BR1 BL1 BL2 TR1 AM BL3 BL4 BR1 BL1 BL2 TR1 AM1 Thermal degradation of ferulic and p-coumaric acids + lignins during roasting
19 B. Special malts investigation
20 Malting procedure Steeping Caramel malt RASTER Green malt Germination Kilning Pilsen malt TRREFACTR Chocolate malt
21 5 EBC 50 EBC Green malt kilning 15 min 125 C 500 EBC 10 min 180 C 900 EBC 1500 EBC PILSEN MALT TRREFACTR step : until 225 C 10 min 225 C 20 min 225 C
22 Emergence of 3 specific profiles
23 Evolution 4-methylphenol, guaiacol and 4-methylguaiacol Temperature of roasting (>250 C) Specific to the use of torrefied malts
24 H p-coumaric acid C 2 4-vinylphenol 2 p-hydroxybenzaldehyde H C 2 Ferulic acid 4-vinylguaiacol vanillin 2 H 2 H p-hydroxybenzaldehyde apocynol C 4-methylphenol C 4-methylguaiacol 2 Phenol guaiacol Me H Me sinapic acid Me Me syringaldehyde acetovanillon H H o-coumaric acid salicylaldehyde
25 Evolution of vanillin, 4-ethylguaiacol, 4-ethylphenol and apocynol.
26 Evolution of 4-vinylphenol and 4-vinylguaiacol.
27 Concentration (µg/l) Phenols glucosides : vanillin? BF 3M 6M 14M
28 Phenols glucosides : extraction procedure ctyl-glucopyranoside 1ml 1000ppm IST Beer or EBC wort - Washing with H 2 mq, 5x5 ml Ether diéthylique - Elution : 5x5mL méthanol Dryness + 50 ml acetate buffer β-d-glucosidase ctanol 3x L:L diethyl ether + dodecane 1ml, 20ppm (EST) Concentration to 500 µl
29 (a) Abundance Released IST µv.s Vanillin µv.s Time (min) (b) Abundance Released IST µv.s Vanillin µv.s Figure 4. Time (min)
30 Volatile monophenols in belgian special beers 5. Conclusions
31 As suspected by beer analysis Use of dark specialty malts liberate specific phenolic compounds These compounds are present in beers produced with torrefied malts Malting process and thermic steps are key factors Investigation of glucosides
32 Thank you for your attention
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