EXTRACTION OF SEDIMENTS FOR AROMATIC AND CHLORINATED HYDROCARBONS
|
|
- Elfrieda Bailey
- 5 years ago
- Views:
Transcription
1 EXTRACTION OF SEDIMENTS FOR AROMATIC AND CHLORINATED HYDROCARBONS Juan. A. Ramirez, Bo Wang, Donell S. Frank, Thomas. J. McDonald, Rebecca Price, Susanne J. McDonald and James M. Brooks TDI-Brooks International./B&B Laboratories Inc. College Station, Texas ABSTRACT Determining organic contaminant levels in sediments requires extraction, isolation and concentration from the matrix. Sediment samples are dried, powdered and homogenized prior to extraction. Sediments are extracted in dichloromethane using a Dionex ASE200 Accelerated Solvent Extractor. The extracts are concentrated and then purified using alumina/silica gel chromatography columns. The resultant eluent is concentrated to a final volume of 1.0 ml and submitted for the determination of aromatic and chlorinated hydrocarbons by gas chromatography/mass spectrometry and gas chromatography/electron capture detection. 1.0 INTRODUCTION The procedure described is capable of extracting, isolating, purifying and concentrating aromatic and chlorinated hydrocarbon contaminants at the parts per billion or parts per trillion level in sediments. Sediment samples are thawed and homogenized. An aliquot of 1-2 g is removed for percent moisture determination. Another aliquot is oven dried at 40 C prior to extraction. Once samples are dried they are ground and homogenized using a mortar and pestle. Approximately 15 g of dry sediment is extracted with 100% dichloromethane using a Dionex ASE200 Accelerated Solvent Extractor operated at 100 C and 2,000 psi. The extracts are concentrated to 1 ml by evaporative solvent reduction in a C water bath. The concentrated sediment extract is purified using alumina/silica gel column chromatography. The extracts are once again concentrated to 1 ml and submitted for instrumental analysis. 2.0 APPARATUS AND MATERIALS 2.1 EQUIPMENT Dionex, ASE200 Accelerated Solvent Extractor (ASE) with 33 ml extraction cells Sediment Extraction for Hydrocarbons - 1
2 Water bath, capable of maintaining a temperature of C Balance, top loading, tare capacity to 300 g, capable of weighing to 1 mg Microbalance, capable of weight to 1 µg Calibrated weights, certified Combustion furnace, electric capable of combusting glassware at 400 C for at least 4 hours Oven, capable of 40 C temperature maintenance Conditioning oven, electric, gravity convection, capable of maintaining a stable temperature of up to 200 C Tumbler, Lortone rock tumbler or equivalent Collection vials, 60 ml certified pre-cleaned with open screw caps and Teflon lined VOA septa Micropipettors, calibrated, 1% accuracy, disposable tips 250 ml flat bottom, boiling flasks Borosilicate glass chromatography columns, 300 mm x 19 mm, with Teflon stopcock Kurderna-Danish (K-D tubes), 25 ml, slow dry concentrator tubes Synder columns, 3 ball Boiling chips, Teflon Glass wool 2.2 REAGENTS Water, gas chromatography/hplc grade or equivalent purity Acetone, pesticide grade or equivalent purity Dichloromethane, pesticide grade or equivalent Hexane, pesticide grade or equivalent Pentane, pesticide grade or equivalent Copper, granular, purified with hydrochloric acid Hydrochloric acid Sodium sulfate, purified by combusting at 400 C for at least 4 hours and stored at 120 C. Alumina, mesh, basic, purified by combusting at 400 C for at least 4 hours and stored at 120 C Silica gel, grade 923, mesh, purified in an oven at 170 C for at least 16 hours and store at 170 C Nitrogen, 99.8% purity 3.0 PROCEDURE All glassware used to extract sediments is washed, solvent rinsed or combusted at 400 C for at least 4 hour. All other items that come in contact with samples are washed and solvent rinsed with acetone and dichloromethane. Sediment samples are thawed and Sediment Extraction for Hydrocarbons - 2
3 homogenized. A portion (1-2 g) is removed for percent moisture determination (see Dry Weight Determination of Sediments). Samples are dried in an oven at 40 C and then ground and homogenized using a motar and pestle. Approximately 15 g of the dried sediment is extracted. Sediments are extracted with dichloromethane using an ASE200. Dried samples are loaded into 33 ml stainless steel ASE extraction cells. Appropriate surrogate and spikes are added on top of the samples. Quality control samples (e.g., blanks, duplicates, matrix spikes and standard reference materials) are prepared in the same manner as samples. The ASE extractor tubes are sealed and placed on the ASE cell carousel. The ASE conditions are 100% dichloromethane as the extraction solvent, 2,000-psi solvent pressure, 100 C cell temperature, and 2 static cycles for 2 minutes each. Extracts are collected in 60 ml collection vials. The extracts are concentrated to approximately 10 ml in the 60 ml collection vials in a C water bath. Extracts are then quantitatively transferred to Kurderna-Danish (K-D) tubes and concentrated to 1.0 ml in a C water bath. Extracts are purified using alumina/silica gel chromatography columns. Borosilicate glass columns (300 mm x 19 mm) are packed with conditioned sodium sulfate, alumina and silica gel. Combusted and cooled alumina is deactivated by adding 1% (w/w) reagent water and tumbled for at least 1 hour using a Lortone rock tumbler. Combusted, cooled silica gel is deactivated by adding 5% (w/w) reagent water and tumbled for at least 1 hour using a Lortone rock tumbler. The columns are packed in dichloromethane with glass wool, 1-2 g of cooled sodium sulfate, 10 g of deactivated alumina, 20 g of deactivated silica gel and another 1-2 g of sodium sulfate. The dichloromethane is drained to the top of the column and replaced with 50 ml of pentane. The pentane is drained to the top of the upper sodium sulfate layer and discarded. The sample extract (approximately 1 ml) is loaded on top of the column and flushed with 200 ml of a 50:50 mixture of pentane and dichloromethane at a flow rate of 1 ml/min. The eluent is collected in a 250 ml flatbottom flask. The eluent is concentrated to approximately 10 ml in a C water bath. The extract is transferred to 25 ml K-D tubes and concentrated. The dichloromethane is exchanged to hexane and concentrated to a final volume of 1 ml. The concentrate is transferred to 2 ml amber vials containing pre-cleaned copper granules and stored at 20 C until analysis. High sulfur containing samples may require additional copper treatment. Figure 1 shows a flow chart of the extraction and purification procedure. Sediment Extraction for Hydrocarbons - 3
4 SAMPLE RECEIPT AND STORAGE SAMPLE PREPARATION % MOISTURE DETERMINATION SAMPLE EXTRACTION SAMPLE CLEANUP VIA ALUMINA/SILICA GEL COLUMN EXCHANGE DICHLOROMETHANE TO HEXANE ANALYSIS BY GC/MS (PAHS) ANALYSIS BY GC/ECD (OCS) Figure 1. Methodology for Extraction, Isolation and Quantification of Sediment Samples for Aromatic and Chlorinated Hydrocarbons. Sediment Extraction for Hydrocarbons - 4
5 4.0 QUALITY CONTROL (QC) All reagents used are verified to be contaminant free. All equipment and glassware used to extract samples are thoroughly cleaned by solvent rinsing or combustion at 400 C. The calibration and accuracy of balances, weights, pipettors and thermometers are checked daily. The calibration and accuracy of balances, weight, pipettors and thermometers are verified yearly by an independent source. All samples are shipped and received under chain-of-custody. A series of quality control samples are processed with each batch of 20 samples or less. The following quality controls are used to ensure the accuracy and precision of sediment data. Surrogates. Solutions containing analytes that do not interfere with the analytes of interest are prepared at concentrations approximately 5 to 10 times the method detection limit (MDL). Specified surrogates are added to each sample extracted, including QC samples, at a specified volume (typically 100 µl) immediately prior to extraction. Method Blank. Method blanks are extractions of all support material used for extraction of samples, with the exception of sediment. A method blank is analyzed with each extraction batch of 20 or fewer samples. The method blank is extracted and analyzed in a manner identical to samples. Matrix Spike. Matrix spikes are extractions of sample matrix fortified with spikes of selected target analytes. A matrix spike and matrix spike duplicate are analyzed with each extraction batch of 20 or fewer samples. Matrix spikes are extracted and analyzed in a manner identical to samples. Laboratory Duplicates. A sample is analyzed in duplicate with each extraction batch of 20 or fewer samples. Standard Reference Material. A standard reference material from the National Institute of Standards and Technology (NIST) is analyzed with each extraction batch of 20 or fewer samples. Sediment Extraction for Hydrocarbons - 5
EXTRACTION OF SEDIMENTS FOR BUTYLTINS
EXTRACTION OF SEDIMENTS FOR BUTYLTINS Juan A. Ramirez, Donell S. Frank, Susanne J. McDonald, and James M. Brooks TDI-Brooks International/B&B Laboratories Inc. College Station, Texas 77845 ABSTRACT Determining
More informationEXTRACTION PROCEDURE
SPE Application Note for Multiresidue Exraction and Clean Up from Fruit and Vegetables This note outlines solid phase extraction (SPE) methodology for the multiresidue extraction and clean up of fruits
More informationAnalytical Method for Coumaphos (Targeted to agricultural, animal and fishery products)
Analytical Method for Coumaphos (Targeted to agricultural, animal and fishery products) The target compound to be determined is coumaphos. 1. Instruments Gas chromatograph-flame thermionic detector (GC-FTD)
More informationDetermination of Melamine Residue in Milk Powder and Egg Using Agilent SampliQ Polymer SCX Solid Phase Extraction and the Agilent 1200 Series HPLC/UV
Determination of Melamine Residue in Milk Powder and Egg Using Agilent SampliQ Polymer SCX Solid Phase Extraction and the Agilent 1200 Series HPLC/UV Application Note Food Safety Authors Chen-Hao Zhai
More informationTitle: Reagents Copy No: ## SOP No.: 3.13/3.1/S Effective Date: July 8, 2013 Location: ### Reagents
QSM Approval: Reagents 1. Introduction Laboratory reagents are used in all procedural steps during the course of sample preparation. To ensure that reagents are of known quality and purity and free of
More informationExtraction of Phenolic Acids from Plant Tissue Using Accelerated Solvent Extraction (ASE )
Application Note 357 Extraction of Phenolic Acids from Plant Tissue Using Accelerated Solvent Extraction (ASE ) Introduction Polyphenols are a large family of metabolic compounds that occur naturally in
More informationASSET EZ4-NCO Dry Sampler Extraction Procedure.
ASSET EZ4-NCO Dry Sampler Extraction Procedure. Michael Halpenny Jamie Brown March 2013 Rev.1.1 sigma-aldrich.com/analytical 1 Abstract: This presentation introduces and details the procedure used for
More informationRESOLUTION OIV-OENO ANALYSIS OF VOLATILE COMPOUNDS IN WINES BY GAS CHROMATOGRAPHY
RESOLUTION OIV-OENO 553-2016 ANALYSIS OF VOLATILE COMPOUNDS IN WINES BY GAS CHROMATOGRAPHY THE GENERAL ASSEMBLY, In view of Article 2, paragraph 2 iv of the Agreement of 3 April 2001 establishing the International
More informationUse of Accelerated Solvent Extraction (ASE) with Centrifugal Evaporation to Automate Fat Determination in Chocolate
Use of Accelerated Solvent Extraction (ASE) with Centrifugal Evaporation to Automate Fat Determination in Chocolate Aaron Kettle Thermo Scientific, Sunnyvale, CA Overview Purpose: To demonstrate automated
More informationPECTINASE Product Code: P129
PECTINASE Product Code: P129 Enzyme for sample clarification prior to patulin analysis. For in vitro use only. P129/V1/02.06.16 www.r-biopharm.com Contents Page Test Principle... 3 Kit Components... 3
More informationSynthesis 0732: Isolating Caffeine from Tea
Work Completed: 01.22.09 Work Submitted: 02.03.09 Synthesis 0732: Isolating Caffeine from Tea Abstract Caffeine was extracted from instant tea and purified by recrystallization. The yield was determined
More informationDetermination of Pesticides in Coffee with QuEChERS Extraction and Silica Gel SPE Cleanup
Determination of Pesticides in Coffee with QuEChERS Extraction and Silica Gel SPE Cleanup UCT Part Numbers ECMSSC50CT-MP 50-mL centrifuge tube and Mylar pouch containing 4000 mg MgSO4 and 1000 mg NaCl
More informationISO INTERNATIONAL STANDARD. Oilseed residues Determination of oil content Part 2: Rapid extraction method
INTERNATIONAL STANDARD ISO 734-2 First edition 1998-08-15 Oilseed residues Determination of oil content Part 2: Rapid extraction method Tourteaux de graines oléagineuses Détermination de la teneur en huile
More informationBromine Containing Fumigants Determined as Total Inorganic Bromide
Bromine Containing Fumigants Determined as Total Inorganic Bromide Introduction: Fumigants containing bromine, mainly methyl bromide, are used for soil disinfection as well as postharvest treatment of
More informationStudent Handout Procedure
Student Handout Procedure Lab period 1: Reaction: Measure 0.75 g of solid cinnamic acid and 25 ml of your unknown alcohol in a 100 ml round bottom flask. Add a stir bar and stir solution until it is completely
More informationC27 Chromatography. Collect: Column Mortar and pestle Dropper (229 mm) Capillary tube TLC plate Aluminum foil UV light
C27 Chromatography (2017/04/24) Collect: Column Mortar and pestle Dropper (229 mm) Capillary tube TLC plate Aluminum foil UV light Prepare: Green leaves Beaker (30 100 ml) Erlenmeyer flask (50, 125 ml)
More informationThe Determination of Pesticides in Wine
Application Note Abstract According to the state institute for chemical and veterinary analysis of food, Conventionally grown wine grapes are one of the crops most extensively treated with pesticides (CVUA
More informationRapid Analysis of Soft Drinks Using the ACQUITY UPLC H-Class System with the Waters Beverage Analysis Kit
Rapid Analysis of Soft Drinks Using the ACQUITY UPLC H-Class System with the Waters Beverage Analysis Kit Mark E. Benvenuti, Raymond Giska, and Jennifer A. Burgess Waters Corporation, Milford, MA U.S.
More informationOchratoxin A N H. N-{ [(3R)-5-chloro-8-hydroxy-3-methyl-1-oxo-3,4-dihydro-1H-isochromen-7-yl]carbon yl}- L-phenylalanine
chratoxin A H H N H Cl N-{ [(3R)-5-chloro-8-hydroxy-3-methyl-1-oxo-3,4-dihydro-1H-isochromen-7-yl]carbon yl}- L-phenylalanine C 20 H 18 ClN 6 MW: 403.81 CAS No.: 303-47-9 [Summary of ochratoxin A] chratoxin
More informationDNA Extraction from Radioative Samples Grind plus kit Method
DNA Extraction from Radioative Samples Grind plus kit Method 4 th Edition 2017.5.24 To extract DNA from radioactive sediment samples with low biomass, we are currently not allowed to use chloroform or
More informationIdentification of reconstituted milk in pasteurized and UHT milk
Translated English of Chinese Standard: NY/T939-2005 Translated by: www.chinesestandard.net Wayne Zheng et al. Email: Sales@ChineseStandard.net NY Agriculture Industry Standard of The People s Republic
More informationJournal of Chemical and Pharmaceutical Research, 2017, 9(9): Research Article
Available online www.jocpr.com Journal of Chemical and Pharmaceutical Research, 2017, 9(9):135-139 Research Article ISSN : 0975-7384 CODEN(USA) : JCPRC5 The Identification and Quantitation of Thymol and
More informationTi-Pure TITANIUM DIOXIDE DETERMINATION OF UNBRUSHED 325 MESH GRIT, SLURRY METHOD: T
Ti-Pure TITANIUM DIOXIDE DETERMINATION OF UNBRUSHED 325 MESH GRIT, SLURRY METHOD: T4400.300.01 DuPont Titanium Technologies Page 2 of 5 1. Principle A slurry sample of known percent solids is diluted with
More informationFAT, TOTAL (Hydrolysis)
FATTO.01-1 FAT, TOTAL (Hydrolysis) PRINCIPLE The major portions of the native fats in corn starch are bound in a manner as to render them unextractable by the usual methods of solvent extraction. When
More information! " # # $% 004/2009. SpeedExtractor E-916
! "# # $% 004/2009 SpeedExtractor E-916! " # # $% The Genépi plant (Artemisia umbelliformis) grows in alpine areas. It is also cultivated and used to produce a herb liquor. Costunolide is a sesquiterpene
More informationGB Translated English of Chinese Standard: GB NATIONAL STANDARD
Translated English of Chinese Standard: GB5009.6-2016 www.chinesestandard.net Sales@ChineseStandard.net GB NATIONAL STANDARD OF THE PEOPLE S REPUBLIC OF CHINA GB 5009.6-2016 National food safety standard
More informationWORK ORDER NUMBER:
Page 1 of 221 Supplemental Report 1 The original report has been revised to include the Level III deliverables package. WORK ORDER NUMBER: 17-03-0531 AIR SOIL WATER MARINE CHEMISTRY Analytical Report For
More informationCHEM Experiment 4 Introduction to Separation Techniques I. Objectives
1 CHEM 0011 Experiment 4 Introduction to Separation Techniques I Objectives 1. To learn the gravity filtration technique 2. To learn the suction filtration technique 3. To learn about solvent extraction
More informationFast Analysis of Smoke Taint Compounds in Wine with an Agilent J&W DB-HeavyWax GC Column
Application Note Flavors and Fragrances Fast Analysis of Smoke Taint Compounds in Wine with an Agilent J&W DB-HeavyWax GC Column Author Vanessa Abercrombie Agilent Technologies, Inc. Abstract The analysis
More informationSolid Phase Micro Extraction of Flavor Compounds in Beer
Solid Phase Micro Extraction of Flavor Compounds in Beer ANNE JUREK Reducing Carryover in Environmental Water Samples Application Note Environmental Author Anne Jurek Applications Chemist EST Analytical
More information*Level IV report narratives are more detailed than other levels.
Reporting Packages Brooks Applied Labs recognizes that report packages and electronic data deliverables (EDD) should be easy to read, reference, understand, and evaluate while containing all necessary
More informationApplication Note: Analysis of Melamine in Milk (updated: 04/17/09) Product: DPX-CX (1 ml or 5 ml) Page 1 of 5 INTRODUCTION
Page 1 of 5 Application Note: Analysis of Melamine in Milk (updated: 04/17/09) Product: DPX-CX (1 ml or 5 ml) INTRODUCTION There has been great interest recently for detecting melamine in food samples
More informationDetermination of Caffeine in Coffee Products According to DIN 20481
Deteration of Caffeine in Coffee Products According to DI 81 Application ote Food Testing & Agriculture Food Authenticity Author Edgar aegele Agilent Technologies, Inc. Waldbronn, Germany Abstract This
More informationAvocado. recipe or working method? WLODEK. Wlodzimierz S. BOREJSZA-WYSOCKI Ph.D. IR-4 Southern Regional Laboratory Research Director
Avocado recipe or working method? WLODEK Wlodzimierz S. BOREJSZA-WYSOCKI Ph.D. IR-4 Southern Regional Laboratory Research Director Food & Environmental Toxicology Laboratory Institute of Food and Agricultural
More informationZoe Grosser, Vinson Leung, Jim Fenster, Brian LaBrecque Horizon Technology, Inc., Salem, NH USA
Zoe Grosser, Vinson Leung, Jim Fenster, Brian LaBrecque Horizon Technology, Inc., Salem, NH USA To develop an automated SPE method for the extraction of 20 organochlorine pesticides using an established,
More informationSolid Phase Micro Extraction of Flavor Compounds in Beer
Solid Phase Micro Extraction of Flavor Compounds in Beer ANNE JUREK Low Level Detection of Trichloroanisole in Red Wine Application Note Food/Flavor Author Anne Jurek Applications Chemist EST Analytical
More informationExtraction of Multiple Mycotoxins From Animal Feed Using ISOLUTE Myco SPE Columns prior to LC-MS/MS Analysis
Application Note AN804 Extraction of Multiple Mycotoxins From Animal Feed Using ISOLUTE Myco Page 1 Extraction of Multiple Mycotoxins From Animal Feed Using ISOLUTE Myco SPE Columns prior to LC-MS/MS Analysis
More informationGeneral overview of the two stages of the QuEChERS technique. Stage 1: Sample extraction. Stage 2: Sample cleanup
QuEChERS Sample Preparation Procedures cat.# 25847, 25848, 25849, 25850, 25851, 25852, 26123, 26124, 26125, 26126, 26215, 26216, 26217, 26218, 26219, 26220, 26221, 26222, 26223, 26224, 26225, 26226, 26242,
More informationRoyal Society of Chemistry Analytical Division East Anglia Region National Schools' Analyst Competition
Royal Society of Chemistry Analytical Division East Anglia Region 2017 National Schools' Analyst Competition East Anglia Region Heat Thursday 20th April, 2017 School of Chemistry University of East Anglia
More informationExtraction of Acrylamide from Coffee Using ISOLUTE. SLE+ Prior to LC-MS/MS Analysis
Application Note AN796 Extraction of Acrylamide from Coffee using ISOLUTE SLE+ Page 1 Extraction of Acrylamide from Coffee Using ISOLUTE SLE+ Prior to LC-MS/MS Analysis This application note describes
More informationCall for AOAC INTERNATIONAL Collaborator
Call for AOAC INTERNATIONAL Collaborator -- The collaborative study protocol of "High Throughput GC-MS(GC-MS/MS) and LC-MS-MS Method for Multi-classes and Multi-kinds of Residual Pesticides in Tea" 1.
More informationTotal Dissolved Solids: Environmental Express StableWeigh Analytical Testing Vessels. Dr. Edward F. Askew June 15, 2016
Total Dissolved Solids: Environmental Express StableWeigh Analytical Testing Vessels Dr. Edward F. Askew June 15, 2016 Table of Contents Abstract... 4 Introduction... 5 Historical Review of Total Dissolved
More informationCopyright JnF Specialties, LLC. All rights reserved worldwide.
www.quality-control-plan.com/copyright.htm PROCEDURE FOR PREPARING STANDARD REAGENTS, MISCELLANEOUS SOLUTIONS, AND INDICATORS (mo/yr) Revisions Rev: Letter E.O. Number - Description Date Used On Contract#:
More informationYeast nuclei isolation kit. For fast and easy purification of nuclei from yeast cells.
ab206997 Yeast nuclei isolation kit Instructions for use: For fast and easy purification of nuclei from yeast cells. This product is for research use only and is not intended for diagnostic use. Version
More informationAnalysis of Beta-Carotene and Total Carotenoids from Pacific Sea Plasma (Spectrophotometric Method)
Analysis of Beta-Carotene and Total Carotenoids from Pacific Sea Plasma (Spectrophotometric Method) Background: Spirulina has several carotenoids, the major components being β-carotene, zeaxanthin, echinenone,
More informationOverview. Hydrometer Selection. About Specific Gravity. Conditions Affecting Hydrometer Accuracy
2 Hydrometer Selection Specific Gravity (Pg. 4) Precision (Pg. 4) Tall Form High Precision (Pg. 4) Short Form High Precision (Pg. 4) Broad (Pg. 5) Baume (Pg. 5) Narrow (Pg. 5) Broad (Pg. 5) Dual Scale
More informationExperiment 6 Thin-Layer Chromatography (TLC)
Experiment 6 Thin-Layer Chromatography (TLC) OUTCOMES After completing this experiment, the student should be able to: explain basic principles of chromatography in general. describe important aspects
More informationDetermination of Methylcafestol in Roasted Coffee Products According to DIN 10779
Deteration of Methylcafestol in Roasted Coffee Products According to DIN 1779 Application Note Food Testing & Agriculture Food Authenticity Author Edgar Naegele Agilent Technologies, Inc. Waldbronn, Germany
More informationDetermination of Ochratoxin A in Roasted Coffee According to DIN EN 14132
Deteration of Ochratoxin A in Roasted Coffee According to DIN EN 14132 Application Note Food Testing & Agriculture Pesticides, Mycotoxins & Other Contaants Author Edgar Naegele Agilent Technologies, Inc.
More informationE25 ISOLATION OF A BIOLOGICALLY ACTIVE COMPOUND The isolation of caffeine from tea leaves
E25 ISLATI F A BILGICALLY ACTIVE CMPUD The isolation of caffeine from tea leaves ITRDUCTI The overwhelmin majority of bioloically active molecules are oranic compounds, e.. alcohol, salicylic acid and
More informationEDICT ± OF GOVERNMENT
EDICT ± OF GOVERNMENT Inordertopromotepubliceducationandpublicsafety,equal justiceforal,abeterinformedcitizenry,theruleoflaw,world tradeandworldpeace,thislegaldocumentisherebymade availableonanoncommercialbasis,asitistherightofal
More informationTSKgel TECHNICAL INFORMATION SHEET No. 131
TSKgel TECNICAL INFORMATION SEET No. Analysis of Synthetic Sweeteners in Coffee by PLC Synthetic sweeteners are used in many foods because they have fewer calories than sugar. Acesulfame potassium (Acesulfame-K),
More informationHigher Resolution Separation of Organic Acids and Common Inorganic Anions in Wine
Application Note 73 Higher Resolution Separation of Organic Acids and Common Inorganic Anions in Wine INTRODUCTION The flavors imparted by wine are in part due to its organic acid composition. Tartaric,
More informationIntroduction to the General Chemistry II Laboratory. Lab Apparatus and Glassware
Introduction to the General Chemistry II Laboratory Lab Apparatus and Glassware Review the first of two photographs at the end of the Data Documentation section, near the beginning of your lab manual.
More informationPreparation 1: Chloroform
SECTION 3: General Lab Procedures Part 3: The Preparation of General Lab Chemicals General laboratory processes involve those chemical reactions where basic chemicals are being reacted, and produced. General
More informationDensity Measurement of Petroleum Products by Hydrometer Method ASTM D287 - ASTM D IP API ASTM D6822
ASTM D287 - ASTM - IP 160 - API 2547 - ASTM D6822 Small footprint Detachable leakage tray Easy to drain and clean Stainless steel Cylinder visible over full length Wide temperature range General This specific
More informationIntroducing the Automated Fiber Analyzer
Introducing the Automated Fiber Analyzer Performs Crude Fiber, ADF and NDF determinations Automatically adds required solutions and hot water rinses Batch Process up to 24 samples at one time Accurate
More informationDetermination of Alcohol Content of Wine by Distillation followed by Density Determination by Hydrometry
Sirromet Wines Pty Ltd 850-938 Mount Cotton Rd Mount Cotton Queensland Australia 4165 www.sirromet.com Courtesy of Jessica Ferguson Assistant Winemaker & Chemist Downloaded from seniorchem.com/eei.html
More informationDetermination of natamycin in wines Résolution OIV-SCMA
Method OIV-MA-AS323-09 Type IV methods Résolution OIV-SCMA 461-2012 1. INTRODUCTION Different methods for the determination of natamycin are used based mainly on HPLC in combination with DAD or MS detection.
More information5KCM0512A W A_ENv10.indd 1 10/13/15 12:41 PM
5KCM0512A PARTS AND FEATURES PARTS AND ACCESSORIES Scale readout display Timer readout display Plunger handle Power button Lid Plunger (stainless steel mesh filter with silicone seal not shown) Timer button
More information15. Extraction: Isolation of Caffeine from Tea
15. Extraction: Isolation of Caffeine from Tea In this experiment you will isolate a compound from a natural source using two extraction techniques. Such compounds are often referred to as natural products.
More informationDensity Measurement of Petroleum Products by Hydrometer Method ASTM D287 - ASTM D IP API ASTM D ASTM D ISO 3675
ASTM D287 - ASTM - IP 160 - API 2547 - ASTM D3142 - ASTM D6822 - ISO 3675 Small footprint Detachable spill tray Easy to drain and clean Stainless steel Hydrometer visible over full length Wide temperature
More informationAutomation of AOAC for the Identification of FD&C Color Additives in Foods using Solid Phase Extraction
Automation of AOAC 988.13 for the Identification of FD&C Color Additives in Foods using Solid Phase Extraction Keywords Introduction Application Note FB0112 GX-274 ASPEC, AOAC, Spectrophotometer, Food
More information1. Blender: Osterizer, 10-speed, or equivalent. 2. Separatory Funnel: Kilborn or equivalent (see figure 1) 2. HCl Solution: HCl/water (7:93 by volume)
EXTER.01-1 INFESTATION IN WHOLE CORN PRINCIPLE Whole corn is suspended in aqueous borax solution to float insects and insect fragments, which are collected on filter paper for microscopic identification
More informationPART I CENTRIFUGE EXTRACTION METHOD USING CHLORINATED SOLVENT
Test Procedure for DETERMINING ASPHALT CONTENT OF BITUMINOUS MIXTURES BY TxDOT Designation: Tex-210-F Effective Dates: April 2008 October 2016. 1. SCOPE 1.1 Use this test method to determine, by four cold
More informationSeparations. Objective. Background. Date Lab Time Name
Objective Separations Techniques of separating mixtures will be illustrated using chromatographic methods. The natural pigments found in spinach leaves, β-carotene and chlorophyll, will be separated using
More informationMethanol (Resolution Oeno 377/2009, Revised by OIV-OENO 480/2014)
Method OIV-MA-AS312-03A Type II method (Resolution Oeno 377/2009, Revised by OIV-OENO 480/2014) 1. Scope of application This method is applicable to the determination of methanol in wine for concentrations
More informationEXPERIMENT 6. Molecular Fluorescence Spectroscopy: Quinine Assay
EXPERIMENT 6 Molecular Fluorescence Spectroscopy: Quinine Assay UNKNOWN Submit a clean, labeled 500-mL volumetric flask to the instructor so that your unknown quinine solution may be issued. Your name,
More informationBrooks 1250/55 Sho-Rate TM Series Glass Tube Flowmeters
Data Sheet Brooks 1250/55 Sho-Rate Series Brooks 1250/55 Sho-Rate TM Series Glass Tube Flowmeters 1 Brooks 1250/55 Sho-Rate Series Data Sheet Description Series 1250/55 are flowmeters for liquids and gases
More informationAppNote 13/2012. Automated Solid Phase Extraction (SPE)-LC-MS/MS Method for the Determination of Acrylamide in Brewed Coffee Samples KEYWORDS ABSTRACT
AppNote 13/2012 Automated Solid Phase Extraction (SPE)-LC-MS/MS Method for the Determination of Acrylamide in Brewed Coffee Samples Fredrick D. Foster, John R. Stuff, and Edward A. Pfannkoch Gerstel, Inc.,
More informationAnalysis of trace elements and major components in wine with the Thermo Scientific icap 7400 ICP-OES
APPLICATION NOTE 43355 Analysis of trace elements and major components in wine with the Thermo Scientific icap 7400 ICP-OES Authors Sanja Asendorf, Application Specialist, Thermo Fisher Scientific, Bremen,
More informationIn the preparation of this Tanzania Standard assistance was derived from:
TANZANIA BUREAU OF STANDARDS DRAFT TANZANIA STANDARD COCONUT MILK AND COCONUT CREAM SPECIFICATION (DRAFT FOR COMMENT ONLY) AFDC 4 (3761) P3 0 FOREWORD Coconut milk and coconut cream shall be prepared by
More information, FAX
Detecting 2,4,6 TCA in Corks and Wine Using the znose Edward J. Staples, Ph.D. Electronic Sensor Technology, 1077 Business Center Circle, Newbury Park, California, Ph. 805-480-1994, FAX 805-480-1984, Email:
More informationProfiling of Aroma Components in Wine Using a Novel Hybrid GC/MS/MS System
APPLICATION NOTE Gas Chromatography/ Mass Spectrometry Authors: Sharanya Reddy Thomas Dillon PerkinElmer, Inc. Shelton, CT Profiling of Aroma Components in Wine Using a Novel Hybrid GC/MS/MS System Introduction
More informationComparative determination of glycosides in senna by using different methods of extraction (Soxhlet, maceration and ultrasonic bath)
1 Experiment 1, 2 and 3 Comparative determination of glycosides in senna by using different methods of extraction (Soxhlet, maceration and ultrasonic bath) Aim: determine the yield among different extraction
More informationTyler Trent, SVOC Application Specialist; Teledyne Tekmar P a g e 1
Application Note Flavor and Aroma Profile of Hops Using FET-Headspace on the Teledyne Tekmar Versa with GC/MS Tyler Trent, SVOC Application Specialist; Teledyne Tekmar P a g e 1 Abstract To brewers and
More informationA Fast Method for Sugar Analysis of Instant Coffee Samples
A Fast Method for Sugar Analysis of Instant Coffee Samples Sachin Patil and Jeffrey Rohrer Thermo Fisher Scientific, Sunnyvale, CA Application Update 202 Key Words Dionex CarboPac SA10-4μm Column, Electrochemical
More information3. Aspirin Analysis. Prelaboratory Assignment. 3.1 Introduction
In this experiment, you will analyze the purity of your crude and recrystallized aspirin products using a method called thin layer chromatography (TLC). You will also determine the percent yield of your
More informationCHROMATOGRAPHY. Think about food Care about safety. Vol. 1 Melamine.
CRMATGRAPY Think about food Care about safety Vol. 1 Melamine www.mn-net.com Focus on melamine The global production and trading of food and feed products makes monitoring and control difficult. nly analysis
More informationIntroduction. Experimental. : : APPLICATION NOTE SP024: Pesticide Anaylsis: Standard QuEChERS vs Modified Method. : : APPARATUS: Geno/Grinder
Tissue Homogenization Cell Lysis Mixing & Blending A P P L I C A T I O N N O T E Analysis of Pesticides in Fruit and Vegetable Products using a Standard QuEChERS Method and a Modified Method Involving
More informationEDICT ± OF GOVERNMENT
EDICT ± OF GOVERNMENT Inordertopromotepubliceducationandpublicsafety,equal justiceforal,abeterinformedcitizenry,theruleoflaw,world tradeandworldpeace,thislegaldocumentisherebymade availableonanoncommercialbasis,asitistherightofal
More informationION FORCE DNA EXTRACTOR FAST Cat. N. EXD001
ION FORCE DNA EXTRACTOR FAST Cat. N. EXD001 User Manual Via San Geminiano, 4 41030 San Prospero (MO) Italy : +39 059 8637161 : +39 059 7353024 : laboratorio@generon.it : www.generon.it [1] User Manual
More informationChapter 14 Tex-619-J, Analysis of Water for Chloride and Sulfate Ions
Chapter 14 Tex-619-J, Analysis of Water for Contents: Section 1 Overview... 14-2 Section 2 Apparatus... 14-3 Section 3 Reagents... 14-4 Section 4 Procedures... 14-5 Section 5 Calculations... 14-6 Section
More informationMeasuring tannins in grapes and red wine using the MCP (methyl cellulose precipitable tannin assay
Measuring tannins in grapes and red wine using the MCP (methyl cellulose precipitable tannin assay Scope The MCP (methyl cellulose precipitable) tannin assay is a simple and robust means of measuring the
More informationIPPA SAG method revision 0,
IPPA SAG method revision 0, 23.01.2017 This procedure is based on the method described by Cox and Higby, in Food Inds., 16, 441 (1944) and Joseph and Baier, Food Technol. 3, 18 (1949) and is a modification
More informationthree different household steam ovens, representing a number of identically constructed ovens (see attached list at the end of this document):
This is to confirm to BSH Hausgeräte GmbH Carl-Wery-Str. 34 D-81739 München that within a study (reports March 26 th,2015; June 18 th, 2015; July 16 th, 2015) conducted by Universidad Zaragoza (Plant Foods
More informationTHIN LAYER CHROMATOGRAPHY AND MELTING POINT DETERMINATION: DETECTION OF CAFFEINE IN VARIOUS SAMPLES
EXPERIMENT 8 THIN LAYER CHROMATOGRAPHY AND MELTING POINT DETERMINATION: DETECTION OF CAFFEINE IN VARIOUS SAMPLES Additional Resources http://orgchem.colorado.edu/hndbksupport/tlc/tlc.html http://coffeefaq.com/caffaq.html
More informationPractical 1 - Determination of Quinine in Tonic Water
Practical 1 - Determination of Quinine in Tonic Water Introduction Quinine has a fluorescence and a UV absorbance and so can be quantified using either of these. In the method described here the absorbances
More informationBOKELA BACKFLUSH FILTER
BOKELA BACKFLUSH FILTER BOKELA Ingenieurgesellschaft für Mechanische Verfahrenstechnik mbh 76131 Karlsruhe Germany Phone +49 (721) 9 64 56-0 Fax -10 e-mail: bokela@bokela.com Iwww.bokela.com 3 / 2009 e
More informationVINIFI CATION INTÉGR ALE
VINIFI CATION INTÉGR ALE If Tonnellerie Ermitage has chosen to offer the Integral Winemaking method, (Vinification Intégrale ) it is because this system has proved its efficacity for over ten years. Tested
More informationBeyond TPH. John Fitzgerald Massachusetts Department of Environmental Protection
Beyond TPH John Fitzgerald Massachusetts Department of Environmental Protection Characterizing Petroleum Contamination Source Migration Screening: PID, TPH ID/Detailed: GC, GC/MS Risks Posed by Hydrocarbons
More informationAWRI Refrigeration Demand Calculator
AWRI Refrigeration Demand Calculator Resources and expertise are readily available to wine producers to manage efficient refrigeration supply and plant capacity. However, efficient management of winery
More informationDetermination of the concentration of caffeine, theobromine, and gallic acid in commercial tea samples
Determination of the concentration of caffeine, theobromine, and gallic acid in commercial tea samples Janna Erickson Department of Chemistry, Concordia College, 901 8 th St S, Moorhead, MN 56562 Abstract
More informationDraft for comments only Not to be cited as East African Standard
EAST AFRICAN STANDARD Meat and meat products Determination of total fat content EAST AFRICAN COMMUNITY CD/K/720:2010 ICS 67.120.10 EAC 2010 First Edition 2010 CD/K/720:2010 Foreword Development of the
More informationDetermination of Metals in Wort and Beer Samples using the Agilent 5110 ICP-OES
Determination of Metals in Wort and Beer Samples using the Agilent 5110 ICP-OES Authors Application Food and Beverages Dana Sedin 1, Stacey Williams 1, Elizabeth Kulikov 2, Jenny Nelson 3, Greg Gilleland
More informationSeptember is NATIONAL PREPAREDNESS MONTH
Sources of Water in a Disaster Water will become your most precious commodity during any disaster event. Because of our susceptibility to major flooding incidents and the possibility of earthquake, it
More informationQUANTITATIVE ASSAY FOR OCHRATOXIN A IN COFFEE, COCOA, AND SPICES (96-well kit)
QUANTITATIVE ASSAY FOR OCHRATOXIN A IN COFFEE, COCOA, AND SPICES (96-well kit) OCHRATOXIN A Ochratoxin A is a toxic secondary metabolite produced by several molds of the Aspergillus and Penicillium genera,
More informationASTM D6042 Calibration Mixture
Analytical Reference Materials ASTM D6042 Calibration Mixture Catalog # 31628 Lot # A073321 & A066644 110 Benner Circle Bellefonte, PA 16823-8812 (814) 353-1300 FOR LABORATORY USE ONLY. READ MSDS PRIOR
More informationSequential Separation of Lysozyme, Ovomucin, Ovotransferrin and Ovalbumin from Egg White
AS 662 ASL R3104 2016 Sequential Separation of Lysozyme, Ovomucin, Ovotransferrin and Ovalbumin from Egg White Sandun Abeyrathne Iowa State University Hyunyong Lee Iowa State University, hdragon@iastate.edu
More informationPRODUCT SELECTION GUIDE
PRODUCT SELECTION GUIDE REPIPET DISPENSERS Products Repipet Dispenser Reagent Volume (ml) Reservoir Type Accuracy Reproducibility Composition Compatibility Autoclavable Page 0.5, 1, 5, 10 950 ml Round,
More information