Effect of Hibiscus sabdraffira and tea extract on cellular immunity in lab animals

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1 Effect of Hibiscus sabdraffira and tea extract on cellular immunity in lab animals تاثير نبات الكجرات والشاي في المناعة الخلوية في الحيوان المختبري اسهار ع زا نطيف انذهب جايعة بابم كهية انعهوو وس سايي شكز جايعة انكوفة كهية انطب فيي هيذ انةراسية فيى يعزفية فيص يز ان ي ه ان يا ي ن بياا انكايزاا واناياد ااسيو واا يز فيي ان اعية ان هو ية فيي انأييوا ن بيزد ي رهية با بيار انبهع ية فيب ا يشال ويباة زوبهوف زاسونييوو وا بيار انأ اسيية ان يص زت ي يرب با بيار اناهيةتوالكز بياا انكايزاا س يا ت يع و ية وا يأة بيار انبهع ية فأي ي يي و 05. p يقار يية بانايياد وكييا ا بييار اناهيية ا اابيييام ب كييور عبييياا ان أ ييل ا ييزار و ان ييهب وان زولكور ا ية بعة يزور 2442 ساعة ي انأق ت Abstract In this study, the effect of aqueous extract of Hibiscus sabdariffa calyces,black and green tea leaves were studied in lab animals. Cellular immunity which represented by the phagocytes as in the reduction of NBT dye and skin DHT test. Hibiscus extract was showed higher significant NBT reduction in P.05 compare with tea extract and control where's there three plants were stimulated cellular immunity in skin DTH test demonstrated by signs of reaction such as redness,thickness,necrotic,indurations and erythma after 2472 hr. Introduction In the last years there has been significant increase in the use of natural products in health care and there potential application in agriculture, pharmaceutical and food industry are being investigated (Gomez et al.,2008).in fact about 30% of drugs industrialized countries are derived from plants (Kanfman et al,1999).plants play an essential role in the health care needs not only for treatment disease, but also to improve the immunological response against many pathologies. Hibiscus sabdariffa is the member of the family malvaceae,was used for different uses as vegetables,some of oils,refreshing drink and food preserves (Fasoyira et al,2005). The calyces have been found to be rich in vitamin c and other antioxidants such as flavonoids and also minerals (Wong et al,2002 ;Babalola et al,2001 ). Fruits are also rich sources of vitamins and antioxidants, which are essential as health food in the building up of body immune system and preventing disease (CAT,2001.).The tea plant Cammellia sinesis, family is theaceae is a perennial evergreen plant that is semi tree or shrub depending on the environment. Green and black tea are processed differently during manufacturing.green tea is non oxidized where's black tea is fully oxidized. Caffeine the most content of black tea where's catechins in green tea is the most important. The effect of plants are multiple from antitumor,antimicrobial,antimutagenis and immune system immunomodulatory (Chan et al,2006;roberts et al,2005 ;Charles et al,2007 ; DellAlca,2004) Materials and Methods Plant extracts 1gm of tea leaves and Hibiscus calyces with 10 ml of boiling distal water for 1hr at water bath. Aqueous extract were then filtered and cooled at room temperature (Charles et al,2007).

2 Animals Healthy Newzland rabbit(orcyctalagus cuninculus ) about 115 kg. were used as experimental animals. They hands at room temperature in labium condition during experimental condition. Immunization program Animals were divided into 4 groups, 5 animals for each group. Three groups were given 5ml of plant extracts orally and 5 animals were given 5 ml of distal water as normal control daily for three weeks. At the beginning of five week, animals were heart punctured for blood.blood were saved with anticoagulant for cellular test (Al Thahab, 2006). Cellular immune procedures: Nitrobluetetrazolium done according with (Park et al.,1968). test was done as in (Tompkins et al.,1973 ). Statistical analysis Results Statistical analysis was done depending on )Dawed and ALYas,1990) The ability of three plants extract was varied in P.05 Hibiscus extract showed higher effect in phagocyte reduction NBT process compare with tea extract and control. Tea extract was rarely less effect, table (1).In effect of three plants on skin DHT test,there are stimulate T cells showed clearly in tuberculin like mild reaction were noted as redness,erythma,indurations, necrotic,table (2). Discussion Natural products play an important role in the field of new drugs research and development (Jin Ming et al,2003 ). Knowledge of plant biologically active compound and their mechanism of action are desirable not only for the discovery of novel therapeutic agents that would validate folkloric remedies, but also for the design of new active molecules or modification of current drugs against divers maladies. One of important application area is immunotherapy. Plants have been shown to modulate the immune system (Spelman et al,2006 ;Cooper,2007 ).In this study the effect of Hibiscus extract on phagocytes process and delayed type hyper sensitivity as in skin test was clearly. Many researches that proved the plant was induce apoptosis in human leukemia cells and inductive effect on tumor promotion in mouse skin and in human leukemia. Seeds of this plant when breasting in the first six months of life stimulates immune system and protects them from diarrhea and acute respiratory infection (UNICEF,2006; Okasha et al,2008 ). Anthocyanin,flavonoids,glycosides,protocatechuic acid, vitamin c and other substances which are antioxidant so there are improve immune system.green and black tea are posses some contents that share in effects on immune system. Researches improved tea plant is protect against several forms of cancer,cardiovascular disease and microbial infection. Immunomodulatory properties was show in human peripheral blood mononuclear cell (Charles et al,2007 ). Caffeine, polyphenol,saponin,tannin,glycosides in black tea and catechins in green tea that share in effect on immune system varies in stimulatory or suppressor.so in this study we investigated the effect of complementary and alternative medicine plants such as Hibiscus sabdroffira and

3 black and green tea may be immunomodulatory and found that black tea had more effect on T cell compared with others. (Kamath et al,2003 ;Hamant and Yassen,2007 ; Chan et al,2006 ). Table (1) : The effect of plants extract on phagocytes process in rabbits Plant extracts Hibiscus sabdraffira Black tea Green tea Control Mean ± Standard deviations.4240 ± ± ± ± Table (2): test in rabbits P Plants extract Redness Necrotic H.sabdraffira + + Black tea + + Green tea + + Control + : positive effect : negative effect Refrenses indurations(cm) (24 hours).8 indurations (cm) (48 hours).9 indurations (cm) (72 hours).8 Azhar A. L. Al Thahab, (2006). Cryptic Meningitis and some aspects of its local and systemic immunity. Phd. thesis, Babylon University. Babalola,S.O ;Babalola,A.O.,Aworth,o. c. (2001).Compositional attributes of rosella Hibiscus sabdarifa.j.food.technical. Africa.6: CAT(2001).The fruits we eat. Technical center for agricultural and rural cooperation. Washington. The Netherland.pp:920. Chan,C.;Koo, m. Ng,E.;Tang,O. ;Yenng,w.;Ho,p.(2006). Effect of chines green tea on weight,hormonal and biochemical profile in obeses patint with polycystic ovary syndrome J.Soc Gynecol.Investig :13(1). Charles, O.; Michael, V.; Chukwuemeka, S.Damian,C.(2007). Adaptogenic potentials of Camellia sinesis leave,garcinia kola and Kola nitida seed,.scientific ReseaEssay.2(7): Cooper,E. L. ;(2007). The immune system and complemantary and medicine CAM 4(S1):58 Dawed, K.M. and ALYas, Z.A(1990). Statistical Methods in Agricultural Researches. ALMousel University, Iraq. 54. DellAica,I ;Dona,m; Tonello,f. ;Piris,a. ; Mok,M.;(2004).Potent inhibitors of anthrax lethal factor from green tea.embo reports V5(4).

4 Fasoyiro, S.B.; Babalala, S. O.; Owosibo, T. (2005). Chemical composition and sensory quality of fruit flavoured rosella Hibiscus sabdariffa drinks. World. J. of Agricullture Sciences. 1 (2): Gomez, R.;Verastegni,L. ; Quintanella,R.; Tanez,P.,Tanez,R. ; Rodriguez,C.; (2008). Ivn vitro rat lumphocyte proliferationindused by Ocinum basilicum,persea American,Plantago virginica and Rossa spp. Extract.J Medicinal plants researches. Vol.2(1): Hamant and Yaseen,(2007).Immunomodulatory Plants :phytopharmacological Review. Pharmacognosy Reviews.Vol.1,issue 2. JinMing,K.;Ngoh, G.;Lian.C;Tet,C.(2003).Resent advance in tradiotional plant drugs and orchids.acta.pharmacol.sin.24:721. Kamath,A. ;Wong,. ;Das, H. ;Li,,L. ;Reinholl,V. ;Bukowski, j. (2003). Antigen in tea beverage prime human v&2v&2 cells in vitro and in vivo for memory and non memory antibacterial cytokine response ProcNatAcad Sci. V.100(10) Kanfman Mpb.,;Cseke, LJ. ;Waber.S.;Dcuke,JA.;Brekman,HL. (1999).Natural productys from plants.bocaraton.florida:cpc Press Okasha,M; Abubakar,M,;Bako,i. (2008). Study the effect of aqueous Hibiscus sabdarifa Lin seed extract on serum prolactin level of lactating femal albino rats.european J.of Scientific research.issn X Vol22(4): Park, B.H.; Fikring, S.M. and Smith Wick, E.J. (1968). Infection and nitroblue tetrazolium reduction by neutrophils. A diagnostic aid. Lancet 2: Robert,AT., Leviton,L.;Parker, CC,; Grreen,FL.(2005)./ Efeect of plant tea and caffeine on oxygen consumption in human. Med Reviw.(10): Spelman,K. Burn,JJ. Nichols,D. (2006).Modulation of cytokine exsprasion by traditional medicin.areview of herbal immunomodulation.altern,med.review.11: Tompkins, W.A.; Schultz, R. M. and Rama Rao, V.S. (1973). Depressed cell ediated immunity in newborn rabbits bearing fibroma virus induced tumors. Infect. Immun. 7(4): UNICEF, (2006). Areport card on nutrition progress for children. Wong,P.;Yusof,S.;Ghazan,H.;Cheman, Y.(2002).Physico.chemical caharecteristic of rosella Hibiscus sabdarifa.nutr. and Dood.Sci.32:6873.

A BEGINNER S GUIDE TO TEA. Types of Tea, Best Steeping Practices and Natural Health Benefits

A BEGINNER S GUIDE TO TEA. Types of Tea, Best Steeping Practices and Natural Health Benefits A BEGINNER S GUIDE TO TEA Types of Tea, Best Steeping Practices and Natural Health Benefits What is Tea? Tea comes from the plant, Camellia Sinensis. It s a sub-tropical plant that grows best in acidic