IR-4 Ornamental Horticulture Program Research Report Cover Sheet

Transcription

1 g.pdf IR4 Ornamental Horticulture Program Research Report over Sheet Researcher(s) Gary hastagner Trial: 2014 WA Affiliation: Washington State University Pullayup WA PhoneNumber: (253) ProjectTitle: Botrytis Efficacy Protocol #: PR# Research Target rop/plant Product EPA Reg. # Production Site Status Botrytis Gray Mold Alibi Flora (A13703G) S Difenconazole + Azoxystrobi Syngenta Field ontainer Botrytis Gray Mold Badge X2 opper Oxychloride + opp IsaGro Field InGround Botrytis Gray Mold BAS703 06F/BAS703 01F Fluxapyrosad + pyraclostrobi BASF Field ontainer Botrytis Gray Mold F9110 F9110 FM Field ontainer Botrytis Gray Mold Medallion Fludioxonil Syngenta Field ontainer Botrytis Gray Mold Mural (A18126B) WDG Benzovindiflupyr + Azoxystro Syngenta Field ontainer Botrytis Gray Mold Pageant 38WG Boscalid + Pyraclostrobin BASF Field InGround Botrytis Gray Mold Prestop Gliocladium catenulatum Str AgBio Field ontainer Botrytis Gray Mold Proud 3 Thyme oil (5.6%) Biohumanetics Field ontainer Botrytis Gray Mold Regalia O5 (MOI10605) Extract of Reynoutria sachali Marrone Bio In Field InGround Botrytis Gray Mold S2200 4S Mandestrobin Valent Field ontainer Botrytis Gray Mold SP WP SP2770 SePro Field ontainer Botrytis Gray Mold SP2773 SP2773 SePro Field ontainer Botrytis Gray Mold Torque 3.6S Tebuconazole leary Field ontainer Botrytis Gray Mold ZeroTol Hydrogen dioxide BioSafe Syste Field InGround

2 Researcher: Gary hastagner and Annie DeBauw Date: 2/9/2015 Project Title: BT114; IR4 Efficacy of Foliar Fungicides to ontrol Botrytis on oral Sunset Protocol #: PRnumbers: 32070, 31355, 31935, 31522, 31534, 32071, Narrative Summary (Results/Discussion) A total of 15 products were evaluated for their effectiveness in controlling Botrytis spp. on containergrown oral Sunset peonies. Very little disease developed on the plants during this trial. Initial isolations taken from diseased plant material on April 23, 2014 confirmed the presence of B. cinerea and a newly identified species (G3PDH) which is similar to B. calthae. Isolations taken on October 29, 2014 confirmed the presence of Botrytis as well as other pathogens such as ladosporium, Rhizoctonia and Alternaria. Although average disease severity ranged from 0.8 to 5.0, there was considerable variability within the data and none of the treatments affected disease development (Table 2). Based on overall quality ratings, none of the fungicides delayed senescence of the plants compared to the nontreated checks (Table 2). Badge X2, Pageant, Mural, Medallion and both SP numbered products had significantly higher residue ratings than nontreated checks (Table 2). No phytotoxicity was observed in this trial. Plot Layout

3 Researcher: Gary hastagner and Annie DeBauw Date: 2/9/2015 Project Title: BT114; IR4 Efficacy of Foliar Fungicides to ontrol Botrytis on oral Sunset Protocol #: PRnumbers: 32070, 31355, 31935, 31522, 31534, 32071, Results Tables Table 1: Treatment list and application dates. Trt # Products PR# Prod/ 100 gal Interval Batch/Lot Number Application Dates 1 1 Noninoculated 2 Inoculated 3 BAS703 01F fl oz 14 day F fl oz 7 day D A 5 Proud qts 7 day HG A 6 S2200 4S fl oz 14 day VT SP WP lbs 7 day None provided A 8 SP lb 14 day None provided 9 SP lbs 14 day None provided 10 Torque 3.6 S fl oz 14 day 13203VL Alibi Flora S fl oz 14 day Mural WDG oz 14 day Pageant 38WG oz 14 day 2236S02EJ 14 Regalia 5O fl oz 7 day Z1303B A 15 ZeroTol gal 7 day None provided A 16 Medallion WDG oz 14 day YGM2l Prestop lbs 21 day W1601 D 18 Badge X lbs 714 day None provided B 1 Dates: 1 = 4/18/14, 2= 4/25/14, 3= 5/2/14, 4 = 5/8/14, 5 = 5/16/14, 6 = 5/22/14, 7 = 5/30/14, 8 = 6/6/14, 9 = 6/16/14, 10 = 6/23/14, 11= 8/28/14, 12= 9/4/14, 13= 9/11/14, 14= 9/19/14, 15= 9/27/14, 16= 10/7/14, 17 = 10/10/14; A = 1, 2, 3, 4, 5, 6, 7, 8, 10, 11, 12, 13, 14, 15, 16; B = 1, 2, 3, 4, 5, 7, 9, 11, 13, 15, 17; = 1, 3, 5, 7, 9, 11, 13, 15, 17; D = 1, 4, 7, 10, 11, 14, 17.

4 Researcher: Gary hastagner and Annie DeBauw Date: 2/9/2015 Project Title: BT114; IR4 Efficacy of Foliar Fungicides to ontrol Botrytis on oral Sunset Protocol #: PRnumbers: 32070, 31355, 31935, 31522, 31534, 32071, Table 2: Effect of foliar fungicides on the level of residues observed on foliage on May 16, disease severity on September 25, and the overall plant quality on October 27, Trt # Products Prod/ 100 gal Interval Residues Disease Severity Plant Quality 1 Noninoculated 0.0 e 2.4 a 2.4 a 2 Inoculated 0.0 e 1.2 a 3.0 a 4 F fl oz 7 day 0.0 e 2.8 a 2.4 a 5 Proud 3 4 qts 7 day 0.0 e 3.0 a 2.0 a 17 Prestop 4.2 lbs 21 day 0.0 e 3.6 a 2.0 a 14 Regalia 5O 4 fl oz 7 day 0.2 de 2.8 a 1.8 a 10 Torque 3.6S 8 fl oz 14 day 0.4 de 1.8 a 2.4 a 11 Alibi Flora 14 fl oz 14 day 0.4 de 0.8 a 2.8 a 15 ZeroTol 1 gal 7 day 0.4 de 2.4 a 2.0 a 3 BAS70301F 8 fl oz 14 day 0.6 de 1.8 a 2.5 a 6 S2200 4S 7.5 fl oz 14 day 0.6 de 2.0 a 2.6 a 12 Mural WDG 7 oz 14 day 1.0 cd 2.2 a 3.2 a 13 Pageant 38WG 14 oz 14 day 1.6 bc 3.6 a 2.4 a 8 SP lb 14 day 1.8 bc 2.4 a 2.0 a 9 SP lbs 14 day 1.8 bc 1.6 a 2.2 a 16 Medallion WDG 8 oz 14 day 1.8 bc 2.2 a 2.0 a 7 SP WP 2.66 lbs 7 day 2.0 b 3.6 a 2.6 a 18 Badge X2 2 lbs 714 day 3.0 a 5.0 a 2.0 a 1 Fungicide residue was rated on a scale of 0 to 3 where 0 = no residue, 1 = slight, 2 = moderate, and 3 = severe residue on foliage. Disease severity was rated on a 0 to 10 scale, where 0 = no disease and 10 = 91 to 100% of the foliage was diseased. Overall plant quality was also rated on a scale of 15 where 1 = dead foliage, 2 = yellow foliage, 3 = yellow/green foliage, 4 = mostly green foliage, 5 = excellent plants with green foliage. Numbers in columns that are followed by the same letter are not significantly different, P=0.05, Tukey's Studentized Range Test.

5 Researcher: Gary hastagner and Annie DeBauw Date: 2/9/2015 Project Title: BT114; IR4 Efficacy of Foliar Fungicides to ontrol Botrytis on oral Sunset Protocol #: PRnumbers: 32070, 31355, 31935, 31522, 31534, 32071, Experiment Information Experimental Design: Randomized omplete Block Number of Reps: 5 Materials & Methods/Recordkeeping Peonies used in this trial were planted in 5 gallon pots in the fall of October, Plants were blocked based on their height at the start of the trial. They were arranged in a container nursery area in rows that were spaced 4 feet apart with pots spaced 36 apart within rows. The plants were overhead irrigated during the growing season. Treatments initially started on April 18, 2014 and were applied on 7, 14, and 21 day intervals (Table 1). Treatments were delayed starting midjune due to the onset of very warm and dry weather that did not favor disease development. Plants were fertilized on June 9 and July 29, 2014 with 150 ppm of Peter s Professional water soluble fertilizer. On August 8, 2014 the plants were topdressed with Apex NPK MAX at a rate of 30 g/pot. The treatments were resumed on August 28, All treatments were applied in the equivalent of 100 gallons of water and the foliage on plants were sprayed to wet. Each plant received approximately 70.5 ml solution at each application. On August 29, 2014 the plants were inoculated with a spore suspension of and Botrytis paeoniae. Periodically during the growing season, disease development was assessed by rating disease severity on a 0 to 10 scale, where 0 = no disease and 10 = 91 to 100% of the foliage was diseased. Isolations were also done from randomly selected symptomatic samples to determine the cause of the leaf spots at the onset of the trial and at the end of the trial. Overall plant quality was also rated on a scale of 15 where 1 = dead foliage, 2 = yellow foliage, 3 = yellow/green foliage, 4 = mostly green foliage, and 5 = excellent plants with green foliage at the end of the trial to determine if any of the treatments affected the rate of plant senescence. Fungicide residue was rated on a scale of 0 to 3 where 0 = no residue, 1 = slight, 2 = moderate, and 3 = severe residue on foliage.

6 Researcher: Gary hastagner and Annie DeBauw Date: 2/9/2015 Project Title: BT114; IR4 Efficacy of Foliar Fungicides to ontrol Botrytis on oral Sunset Protocol #: PRnumbers: 32070, 31355, 31935, 31522, 31534, 32071, Symptomatic Plants on April 23, 2014

7 Researcher: Gary hastagner and Annie DeBauw Date: 2/9/2015 Project Title: BT114; IR4 Efficacy of Foliar Fungicides to ontrol Botrytis on oral Sunset Protocol #: PRnumbers: 32070, 31355, 31935, 31522, 31534, 32071, Name(s) of Personnel onducting Research: Gary hastagner and Annie DeBauw Location of Trial (city/state): WSU Puyallup Research and Extension enter Use Site (greenhouse/shadehouse/field container/etc): ontainer nursery rop History rop ultivar/variety: oral Sunset Date of Planting: October, 2013 Soil Mix: Specialty Soils Professional Growing Media Row spacing 4 Pot spacing: 36 Product(s) applied during experiment (including treatments, fertilizers, etc): PRODUT/ODE PRNUMBERS OMMON NAME MANUFATURER BAS F proprietary BASF F extract of Lupinus FM Proud thyme oil BioHumanetrics S2200 4S mandestrobin BuFarm SP WP proprietary SePRO SP proprietary SePRO Torque 3.6S tebuconazole NuFarm Pageant 38 WG pyraclostrobin (12.8), boscalid (25.2) BASF Alibi Flora S Azoxystrobin (18.2), difenoconazole (11.4) Syngenta Badge X copper oxychloride (16.81), copper Gowan hydroxide (15.36) Medallion fludioxonil Syngenta Mural WDG Azoxystrobin (30), benzovindiflupyr (15) Syngenta Prestop Gliocladium catenulatum strain J1446 Verdera Regalia 5O Extract of Reynoutria sachalinensis Marrone Bio Innovations Zerotol Hydrogen dioxide, peroxyacetic acid BioSafe Systems Peter s Professional Scotts APEX NPK MAX Simplot Professional Products

8 Researcher: Gary hastagner and Annie DeBauw Date: 2/9/2015 Project Title: BT114; IR4 Efficacy of Foliar Fungicides to ontrol Botrytis on oral Sunset Protocol #: PRnumbers: 32070, 31355, 31935, 31522, 31534, 32071, Photos See BT114; IR4 Botrytis Photos file for various disease symptom photos. Data ollected Initial plant height data was taken on April 18, Periodically during the growing season, disease development was assessed by rating disease severity on a 0 to 10 scale, where 0 = no disease and 10 = 91 to 100% of the foliage diseased. Isolations were also done from randomly selected symptomatic samples to determine the cause of the leaf spots. Overall plant quality was also rated on a scale of 15 where 1 = dead foliage, 2 = yellow foliage, 3 = yellow/green foliage, 4 = mostly green foliage, 5 = excellent plants with green foliage at the end of the trial to determine if any of the treatments affected the rate of plant senescence. Visible fungicide residue levels were rated on a scale of 0 to 3 where 0 = no residue, 1 = slight, 2 = moderate, and 3 = severe residue on foliage. Initial isolations taken from diseased plant material on April 23, 2014 confirmed the presence of B. cinerea and a newly identified species (G3PDH) which is similar to B. calthae. Isolations taken from plant material on October 29, 2014 confirmed Botrytis spp. were present as well as other pathogens such as ladosporium, Rhizoctonia, and Alternaria. Data analysis: Data were analyzed using ANOVA (GLM SAS). Treatment means were separated using Tukey s Studentized Range (HSD) Test. Application Equipment: During the growing season, weeds were controlled by hand weeding. All trial treatment applications were applied with a O 2 sprayer equipped with a XR Teejet 8006 VS nozzle at 15 psi in the indicated volume of water. Raw Data See BT114; IR4 Botrytis Data file for raw data Environmental conditions during the experiment: See BT114; IR4 Botrytis Data file for outdoor environmental conditions.