IR-4 Ornamental Horticulture Program Research Report Cover Sheet

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1 pdf IR4 Ornmentl Horticulture Progrm Reserch Report over Sheet Resercher(s) Gry hstgner Tril: 013WA Affilition: Wshington Stte University Pullyup WA PhoneNumer: (53) Emil: ProjectTitle: Botrytis Efficcy PR# Reserch Trget rop/plnt Product EPA Reg. # Production Site Sttus 3153 Botrytis Gry Mold Botrytis cinere Peoni sp. hipco 6019 N/G Iprodione Byer Botrytis Gry Mold Botrytis cinere Peoni sp. Dconil Wether Stik hlorothlonil Syngent Botrytis Gry Mold Botrytis cinere Peoni sp. Decree Fenhexmid SePro 3159 Botrytis Gry Mold Botrytis cinere Peoni sp. Disrm 480S Fluoxstroin OHP 315 Botrytis Gry Mold Botrytis cinere Peoni sp. FM Botrytis Gry Mold Botrytis cinere Peoni sp. Kocide 000 (Griffin) opper hydroxide Griffin Botrytis Gry Mold Botrytis cinere Peoni sp. Medllion Fludioxonil Syngent 3153 Botrytis Gry Mold Botrytis cinere Peoni sp. Plldium yprodinil + fludioxnil Syngent Botrytis Gry Mold Botrytis cinere Peoni sp. Gliocldium ctenultum Str AgBio 3154 Botrytis Gry Mold Botrytis cinere Peoni sp. Thyme oil (5.6%) Biohumnetics 3155 Botrytis Gry Mold Botrytis cinere Peoni sp. SP770 10WP SP770 SePro 3156 Botrytis Gry Mold Botrytis cinere Peoni sp. SP773 SP773 SePro 3157 Botrytis Gry Mold Botrytis cinere Peoni sp. Torque 3.6S Teuconzole lery Botrytis Gry Mold Botrytis cinere Peoni sp. Trinity S Triticonzole BASF 3158 Botrytis Gry Mold Botrytis cinere Peoni sp. V10135 Fenpyrzmine Vlent 574 Botrytis Gry Mold Botrytis cinere Fir, Dougls Pseudotsug menziesii Pgent 38WG Bosclid + Pyrclostroin BASF Field ontiner

2 Ornmentl Horticulture Progrm Reserch Report Form Resercher: Gry hstgner nd Annie DeBuw Dte: 9/5/0133 BT113; IR4 Efficcy of Mngement Tools forr Botrytis on PRnumers: 574, 3153, 31533, 3159, 315, 31534, 3153, 31535, 3154, 3155, 3156, 3157, 31531, 3158, 31686, Nrrtive Summry (Results/Discussion) Although Botrytis peoniee nd Botrytis cineri weree ssocited with irregulr shped lef lesions nd shoot light/dieck on plnts during this tril, insufficient disese cused y these Botrytis spp. developedd to evlutee the control of these pthogens (Tle ). However, we were le to evlute the effectiveness of the fungicide tretments in controlling red spot or lotch (Figure 1), which is cused y ldosporium peonie. The ANOVA nlysis of dt in this test indicte tht there ws significnt (P< <0.05) tretment effect on the severity of red spot tht developed on leves. Averge tretmentt severity rnged from 0. to 5.5. All of the tretments, except Medllion,, hipco 6019, SP770 nd reduced the severity of diseses compred to the nontreted check (Tle ). Torque, Pgent, Disrm, Dconil WetherStik, Kocide, Trinity, Plldium, nd SP773 were the most effective mterils tested. Kocide nd Dconil Wether Stik were the only products tht resulted in visile residues on the plnts (Tle ). ompred to the nontreted checks, none of the tretments hd ny effect on overll vigor off the plnts in this tril (Tle 3). Figure 1. Red lotch cused y ldosporium peonie

3 Ornmentl Horticulture Progrm Reserch Report Form Resercher: Gry hstgner nd Annie DeBuw Dte: 9/5/0133 BT113; IR4 Efficcy of Mngement Tools forr Botrytis on PRnumers: 574, 3153, 31533, 3159, 315, 31534, 3153, 31535, 3154, 3155, 3156, 3157, 31531, 3158, 31686, Results Tle Tle 1. Tretment List nd Appliction Dtes Applictions TRT TRT/PRODUTT Noninoculted check Plldium SP770 10WP SP773 Torque V10135 S Disrm 480S Pgent 38WG Trinity S hipco 6019 Decree 50 WDG Medllion WDG Dconil WetherStik Kocide DF PRnumers Rte/100 gl 4 oz 6 oz 4 qurts.66 ls 1.66 ls 14 oz 1 oz 1.5 ls 0.50% 1.4 pints 1 l Dtes Dte 1 = 3// 13, 3/9/13, 4/3/13, 4/1/13, 4/18/13, 4/6/13, 4/6/ 13, 5//13, 5/ /10/13, 5/0/13, 5/31/13, 6/7/13, 6/17/13; Dte = 3//13, 4/3/13, 4/18/13, 5//13, 5/0/13, 6/3/13, 6/17/ /13; Dte 3 = 3//13, 4/1/13, 4/18/13, 5/0/13, 6/ /17/13

4 Ornmentl Horticulture Progrm Reserch Report Form Resercher: Gry hstgner nd Annie DeBuw Dte: 9/5/0133 BT113; IR4 Efficcy of Mngement Tools forr Botrytis on PRnumers: 574, 3153, 31533, 3159, 315, 31534, 3153, 31535, 3154, 3155, 3156, 3157, 31531, 3158, 31686, Tle. Effect of folir fungicides on severity of Redd Spot, Botrytis lef lesions nd shoot light, ndd fungicide residuess on mixed vriety plnting of peonies on July, Severity Rtings Botrytis Lef Shoot Fungicide TRT TRT/PRODUT PRnumers Rte/100 gl Red Spots Lesions Blight Residue Torque 3.6 S Pgent 38WG Disrm 480S Dconil WetherStik Kocide DF Trinity S Plldium 6.5 WG SP773 Decree V10135 S Medllion 50 WDG hipco 6019 N/G SP770 10WP Noninoculted check oz 1.4 pints 1 l 1 oz 6 oz 1.66 ls 4 oz 1.5 ls 0.50%.66 ls 4 qurts d d cd cd cd cd cd cd c c c c c c Numers in ech column followed y the sme letter re nott significntly different, P= =0.05, Tukey s Studentized Rnge Test. Rted on scle of (010) where: 0 = none, 1 = 110%, = 110%, 100 = 91100% folige exhiiting symptoms of red lef spot or Botrytis lef lesions. Botrytis shoot light ws rted on 010 scle: where: 0 = none, 1 = 110%, = 110%, 100 = 91100% of the shootss on the plntss exhiited symptoms of shoot dieck. 3 Fungicide residue rting (03) where 0 = none, 1 = slight, = moderte, 3 = severe fungicide residue.

5 Ornmentl Horticulture Progrm Reserch Report Form Resercher: Gry hstgner nd Annie DeBuw Dte: 9/5/0133 BT113; IR4 Efficcy of Mngement Tools forr Botrytis on PRnumers: 574, 3153, 31533, 3159, 315, 31534, 3153, 31535, 3154, 3155, 3156, 3157, 31531, 3158, 31686, Tle 3. Effect of folir fungicides on vigor of plnt vigor in mixed vriety plnting of peonies on July, TRT 7 TRT/PRODUT Torque 3.6 S PRnumers 3157 Rte/100 gl Vigor.0 14 Medllion 50 WDG Disrm 480S Trinity S oz. 3 Plldium 6.5 WG oz oz. 13 Decree ls. 16 Dconil WetherStik pints SP ls 4 qurts Noninocul ted check.3 10 Pgent 38WG oz.4 17 Kocide DF l.4 8 V10135 S hipco 6019 N/G SP770 10WP ls 0.50% Numers in ech column followed y the sme letter re not significntly different, P=0.05, Tukey s Studentizedd Rnge Test. Vigor (13) where: 1 = plnt < 1" tll, = plnt 1"5" tll, 3 = plnt > 5" tll

6 Ornmentl Horticulture Progrm Reserch Report Form Resercher: Gry hstgner nd Annie DeBuw Dte: 9/5/0133 BT113; IR4 Efficcy of Mngement Tools forr Botrytis on PRnumers: 574, 3153, 31533, 3159, 315, 31534, 3153, 31535, 3154, 3155, 3156, 3157, 31531, 3158, 31686, Mterils & Methods/Rec cordkeeping Nme(s) of Personnel onducting Reserch: Gry hstgner nd Annie DeBuw Loction of Tril (city/stte): ommercil Grower s Field locted in Sumner, WA Use Site (greenhouse/shdehouse/field continer/etc): Field rop History rop ultivr/vriety: Mixed Vriety y Plnting cell: 15 ft # Plnts per plnting cell: 45 Plnt spcing: ft Experiment Informtion Experimentl Design: Numer of Reps: Rndomized Split Block 5 Mterils & Methods: Mterils & Methods: This field tril ws loctedd t commercil grower s field in the Puyllupp River Vlley in Sumner, WA. The plnting is 4yerold mixed peony plnting with field rows spced 5 prt nd 850 long. Plnts re spced on centers. This tril consisted of 17 tretments with 5 replictions. Ech repliction ws 15 long nd contined 4 to 5 plnts or crowns per plnting cell. Weeds weree initilly controlled with spot ppliction of % Rnger Pro solution on Mrch 19, 013 using Solo ckpck spryer equipped with 800 TeeJet nozzle. Susequent weed mngement ws done y hnd cultivting or with trctor. The first fungicide ppliction ws mde on Mrch, 013 nd the lst ppliction ws mde on June 17,, 013 (see Tle 1 for specific intervls nd dtes). Fungicides were pplied with O spryer t 15 psi in 100 gllons of wter. Spryer ws equipped n 800 TeeJet nozzle. Plnts were spryed to the point of drip. A nontreted check ws included in ech lock nd disese development ws the result of nturlly occurring inoculum in the field. On verge ech plnt received 50 mls of fungicide solution per ppliction. Finl disese severity dt ws collected onn July, 013.

7 Ornmentl Horticulture Progrm Reserch Report Form Resercher: Gry hstgner nd Annie DeBuw Dte: 9/5/0133 BT113; IR4 Efficcy of Mngement Tools forr Botrytis on PRnumers: 574, 3153, 31533, 3159, 315, 31534, 3153, 31535, 3154, 3155, 3156, 3157, 31531, 3158, 31686, Product(s) pplied during experiment (including tretments, fertilizers, etc): PRODUT/ODE Pgent hipco 6019 N/G Decree 50 WDG Disrm 480S Plldium 6.5 WG Torque 3.6 S Medllion 50 WDG Trinity S SP770 10WP SP773 V10135 Dconil WetherStik Kocide DF Rnger Pro PRNUMBERS OMMON NAME pyrclostroin (1.8). osclid (5.) iprodione fenhexmidd fluoxstroin extrct of Lupinus cyprodinil & fludioxinil teuconzole fludioxonil triticonzole Gliocldium ctenultum thyme oil proprietry proprietry proprietry chlorothlonil copper hydroxide Glyphostee MANUFATURER BASF OHP Aryst OHP FM Syngent lery hemicls Syngent BASF AgBio BioHumnetrics SePRO SePRO Vlent Syngent Griffin orp Monsnto

8 Ornmentl Horticulture Progrm Reserch Report Form Resercher: Gry hstgner nd Annie DeBuw Dte: 9/5/0133 BT113; IR4 Efficcy of Mngement Tools forr Botrytis on PRnumers: 574, 3153, 31533, 3159, 315, 31534, 3153, 31535, 3154, 3155, 3156, 3157, 31531, 3158, 31686, Photos See next pge for photos of field lyout nd tretment effects. Dt ollected Initil emergence dt ws tken on Mrch 1, 013 just priorr to first fungicide ppliction. On July, 103 dt were collected on ldosporium red spot, irregulr shped Botrytis lef lesions nd Botrytis shoot light, visile fungicide residue, plnt vigor, nd overll plnt helth which included plnt color. Rting Scles were s follows: Red spot severity ws rted on scle of 010 where : 0 = none, 1 = 110% %, = 110%, 10 = 91100% plnt covered with purple spots nd lesions. Irregulr shped Botrytis lef lesions were rted onn scle of 010 where: 0 = none, 1 = 110%, = 110%, 10 = 91100% plnt covered with symptom. Shoot light ws rted on scle of 010 where: 0 = none, 1 = 110%, = 110%, 10 = % of the shoots on the plnts exhiited symptoms of Botrytis shoot dieck. Visile fungicide residues were rted on scle off 03 where: 0 = none, 1 = slight, = moderte, 3 = severe fungicide residue evident. Vigor ws rted on scle of 13 where: 1 = plntt < 1" tll, = plnt 1" "5" tll, 3 = plnt > 5" tll Isoltions from symptomtic plnt tissue were collected throughout the tril. Red lef spot, which is cused y ldosporium peonie ws the most prevlent disese tht developed during this tril. Both Botrytis peonie nd Botrytis cineri were recovered from folige with irregulr shped tncolored lef lesions nd shoots tht exhiited light/dieck symptoms. Appliction Equipment: All tril tretment pplictions were pplied with O spryer equipped with n 800 Tee Jet nozzle t 15 psi 100 gllons of wter Rw Dt See BT113 Botrytis IR4 013 file for rw dt Environmentl conditions during the experiment: See BT113 Botrytis IR4 013 file for environmentl dt.

9 13017 Botrytis Efficcy hstgner/debuw Residue: Week 6 Shoot Blight: Week 9 Field Lyout: Week 9 Irregulr Shped Lesion: Week 19 Red Spot Lesions: Week 19