ISOPYRAZAM (249) The first draft was prepared by Dr G Ye, Institute for the Control of Agrochemicals, Ministry of Agriculture, Beijing China

Transcription

1 68 ISOPYRAZAM (49) The first drft ws prepred by Dr G Ye, Institute for the Control of Agrochemicls, Ministry of Agriculture, Beijing Chin EXPLANATION is brod-spectrum folir fungicide belonging to the chemicl clss of ortho-substituted phenyl mides. It controls wide rnge of fungl pthogens. ws first evluted by the JMPR s new compound in ±. An ADI of.6 mg/kg bw/dy nd n ARfD of. mg/kg bw were estblished. The residue definitions were estblished: for complince with MRL for plnt commodities: (sum of syn-isomer nd nti-isomer); for risk ssessment for plnt commodities: Sum of isopyrzm nd -difluoromethyl--methyl-h-pyrzole-4-crboxylic cid [9-(-hydroxyl-- methylethyl)-(rs, 4RS, 9RS)-,,,4-tetrhydro-,4-methnonphthlen-5-yl] mide (CSCD459488) expressed s isopyrzm; for niml commodities (enforcement nd risk ssessment): (sum of syn-isomer nd nti-isomer). The residue is ft-soluble. ws scheduled by the 48 th Session of the CCPR s dditionl MRLs for considertion by the JMPR. The Meeting received informtion on registered lbels nd supporting residue trils dt for pome fruit, stone fruit, cucurbits, pepper, tomto, crrot, brley, whet, oilseed rpe nd penut, s well s storge stbility of isopyrzm nd CSCD during processing. Storge stbility under frozen conditions The Meeting received study report on storge stbility of metbolite CSCD Residues in Crops stored deep frozen for up to 8 Months (Gemrot, F.,, Report No. T495-5-). The stbility of CSCD residues ws tested between nd in whet grin nd strw, oilseed rpe (seed), pple fruit, lentils seed, ornge fruit, spinch leves nd crrot root, covering representtives of ll five crop types i.e. predominntly high wter, cid, oil, protein nd strch contining mterils. g crop smples were fortified with known mount of CSCD in cetonitrile t rte of.5 mg/kg. The solvent ws llowed to evporte nd the smples seled nd stored in freezer t nominl temperture of - ± 5 C. The initil concentrtion ws determined by nlysis of three freshly-prepred fortified smples. At subsequent storge s of pproximtely, 6,, nd 8 months smple set of ech substrte, consisting of control smple, two freshly fortified smples nd two freezer-stored fortified smples, were nlysed for residues of CSCD using method GRM6.A. The test results re shown in tble.there ws no significnt chnge in the residue level of CSCD in ny commodity during the 8 month storge period. Therefore, residues of CSCD re expected to be stble in ll crop commodities when stored t - ± 5 C for t lest 8 months. Tble Stbility of CSCD residues in vrious crops following frozen storge Storge Period (months) CSCD Concentrtion Uncorrected Men Procedurl Recovery (%) b CSCD Concentrtion Corrected c Whet (grin) Whet (strw) Percentge Recovery d

2 68 Storge Period (months) CSCD Concentrtion Uncorrected Men Procedurl Recovery (%) b CSCD Concentrtion Corrected c Oilseed rpe (seed) Apple (fruit) Lentils (seed) Ornge (fruit) Spinch (leves) Crrot (roots) Men of two smples (three t months), not corrected for procedurl recovery b Men of two recoveries c Men of two smples (three t months), corrected for procedurl recovery d Percentge of dy = (residue concentrtion / initil residue concentrtion using corrected residues) x Percentge Recovery d USE PATTERNS The registered GAP informtion is summrised in following tble. Tble Use pttern (GAP) for isopyrzm Crop Country or region Method Mx No of Apps App Intervl (dys) App Rte (g i/h) Pome fruit (outdoor) Chile Folir spry 5 Min PHI (dys)

3 68 Crop Country or region Method Mx No of Apps App Intervl (dys) App Rte (g i/h) Min PHI (dys) Apricot nd pech (outdoor) Turkey Folir spry -4 5 (pech) (pricot) Cucumber nd courgette Europe Folir spry 5 g i/h (protected) (UK) Melon nd wtermelon Europe Folir spry 5 (protected) (UK) Pepper, sweet nd chili Europe Folir spry 5 (protected) (UK) Tomto nd ubergine Europe Folir spry 5 (protected) (UK) Crrot Europe Folir spry (outdoor) (UK) Brley, whet, rye nd Europe Folir spry 4 5 n/ triticle (outdoor) (UK) Oilseed rpe Europe Folir spry n/ 5 n/ (outdoor) (UK) Penut (outdoor) Ltin Americ (Nicrgu) Folir spry per crop cycle (4 per yer) 4-5 (mx. 5 g i/h per yer) Not pplicble - defined by the vegettion period from ppliction until hrvest. N.B. Ltest time of ppliction is BBCH 6-69 (end of flowering). ( ): N.B. The crop cycle for penut (i.e. plnting to hrvest) tkes c. 4-5 months. RESIDUES FROM SUPERVISED FIELD TRIALS ON CROPS Apple A totl of sixteen supervised trils were conducted on pple in Europe (Frnce, Germny, Greece, Hungry, Itly, Polnd, Portugl nd Spin) in. The trils support the registered GAP for pome fruit in Chile. Smples of pple whole fruits were collected on the sme dy of ppliction immeditely fter the spry deposit hd dried nd pproximtely,, 4 nd dys fter the finl ppliction. Smples were immeditely frozen nd mintined in frozen storge for periods of up to months prior to extrction. Residues of isopyrzm (both isomers) in pple were determined using nlyticl method GRM6.B with n LOQ of.5 mg/kg for ech isomer (. mg/kg for the sum of both isomers). Residues of CSCD in pple were determined using nlyticl method GRM6.A with n LOQ of.5 mg/kg. The results of the trils re summrised in tble, where residues relevnt to risk ssessment nd the setting of n MRL for pome fruit re underlined Tble Residues in pple (outdoor) from supervised trils in Europe involving folir pplictions of ( g/l SC formultion) Apple Appliction DALA Tril Loction Country, yer (Vriety) Tril: S-4- Brnim, Brndenburg,Germny, (Idred) Tril: S-4- Stde, Niederschsen,Germny, (Elstr) Rte (g i/h) dy dy Wter (L/h) No. (dys) 4 4 Residues CSCD Totl b S-4 S-4 (SC) S-4 S-4 (SC)

4 684 Apple Appliction DALA Tril Loction Country, yer (Vriety) Rte (g i/h) Wter (L/h) No. (dys) Residues CSCD Totl b Tril: S-4- Frnkfurt, Brndenburg,Germny, (Shmpion) Tril: S-4-4 Stde, Niederschsen Germny, (Elstr) Tril: S-4-5 Szmotulski, Wielkopolskie, Polnd, (Szmpion) Tril: S-4-6 Obornicki, Wielkopolskie, Polnd, (Golden Delicious) Tril: S-4- Fejer, Kozep-Dunntul, Hungry, (Idred) Tril: S-4-8 Toln, Del- Dunntul,Hungry, (Jongold) dy dy dy dy 4+ 5 dy dy Tril: S Pell, Kentriki 5 Mkedoni, Greece, dy (Grnny Smith) Tril: S-44- Grd, Lnguedoc- Roussillon, Frnce, (Gl) Tril: S-44- Argon, Zrgoz, Spin, (Golden Delicious) Tril: S-44-4 Zrgoz, Argon, Spin, (Verde Doncell) Tril: S-44-5 Crcedo, Entre Douro Minho, Portugl, (Golden Delicious) dy 6+ 4 dy dy 5+ 4 dy S-4 S-4 (SC) S-4 S-4 (SC) S-4 S-4 (SC) S-4 S-4 (SC) S-4 S-4 (SC) S-4 S-4 (SC) S-44 S-44 (SC) S-44 S-44 (SC) S-44 S-44 (SC) S-44 S-44 (SC) S-44 S-44 (SC)

5 685 Apple Appliction DALA Residues Tril Loction Country, yer (Vriety) Rte (g i/h) Wter (L/h) No. (dys) CSCD Totl b Tril: S Ferrr, Emili Romgn, 5 Itly, dy (Impertore Dllgo) S-44 S-44 (SC) Tril: S Ferrr, Emili Romgn, 64 Itly, dy (Fujiko) S-44 S-44 (SC) Tril: S-44-8 Pell, Kentriki Mkedoni, Greec, (Grnny Smith) 5+ 5 dy S-44 S-44 (SC) Sum of syn- nd nti-isomer (i.e. residue definition for enforcement) b Sum of isopyrzm nd CSCD expressed s isopyrzm (i.e. residue definition for risk ssessment) Stone fruit Apricot nd pech A totl of eight supervised trils were conducted on pricot (4 trils) nd pech (4 trils) in Europe (Frnce, Itly nd Spin) in. The pech trils support the registered cgap in Turkey (i.e. g i/hl; dy PHI for pech). Smples of pech nd pricot whole fruits were collected on the sme dy of ppliction immeditely fter the spry deposit hd dried nd pproximtely, nd dys fter the finl ppliction. Smples were immeditely frozen nd mintined in frozen storge for periods of up to 4 months prior to extrction. Residues of isopyrzm (both isomers) in stone fruit were determined using nlyticl method GRM6.B with n LOQ of.5 mg/kg for ech isomer (. mg/kg for the sum of both isomers). Residues of CSCD in stone fruit were determined using nlyticl method GRM6.A with n LOQ of.5 mg/kg. The results of the trils re summrised in Tbles 4 nd 5. Tble 4 Residues in pech (outdoor) from supervised trils in Europe involving folir pplictions of ( g/l SC formultion). Pech Appliction DALA Residues Tril Loction Country, Yer (Vriety) Rte (g i/h) Wter (L/h) No. (dys) CSCD Totl b Tril: S-498- Longuedoc-Roussillin, Pyrenees-Orientles, Frnce, (Millrdiv) dy w/o stone w/o stone w/o stone w/o stone S- 498 S- 498 (SC)

6 686 Pech Appliction DALA Residues Tril Loction Country, Yer (Vriety) Rte (g i/h) Wter (L/h) No. (dys) CSCD Totl b Tril: S-498- Zrgoz, Spin, (Crlson) dy w/o stone w/o stone w/o stone w/o stone S- 498 S- 498 (SC) Tril: S-498- Zrgoz, Spin, (Ctherine) dy 55 6 w/o stone w/o stone w/o stone w/o stone S- 498 S- 498 (SC) Tril: S Bologn, Itly, (P) dy w/o stone w/o stone w/o stone w/o stone S- 498 S- 498 (SC) Sum of syn- nd nti-isomer (i.e. residue definition for enforcement) b Sum of isopyrzm nd CSCD expressed s isopyrzm (i.e. residue definition for risk ssessment) c Residues clculted using the peel/pulp rtio Tble 5 Residues in pricot (outdoor) from supervised trils in Europe involving folir pplictions of ( g/l SC formultion). Apricot Appliction DALA Residues Tril Loction Country, yer (Vriety) Rte (g i/h) Wter (L/h) No. (dys) CSCD Totl b Tril: S Vlenci, Vlenci, Spin, (Mitxer de Cstelló) dy 44 w/o stone w/o stone w/o stone w/o stone S- 499 S- 499 (SC) Tril: S Vlenci, Vlenci, Spin, (Tdeo) dy 6 49 w/o stone w/o stone w/o stone w/o stone S- 499 S- 499 (SC)

7 68 Apricot Appliction DALA Residues Tril Loction Country, yer (Vriety) Rte (g i/h) Wter (L/h) No. (dys) CSCD Totl b Tril: S Ferrr, Emili Romgn, Itly, (Portici) dy w/o stone w/o stone w/o stone w/o stone S- 499 S- 499 (SC) Tril: S Bologn, Emili Romgn, Itly, (Precoce D'Imol) 5+ 5 dy w/o stone w/o stone w/o stone w/o stone S- 499 S- 499 (SC) Sum of syn- nd nti-isomer (i.e. residue definition for enforcement) b Sum of isopyrzm nd CSCD expressed s isopyrzm (i.e. residue definition for risk ssessment) c Residues clculted using the peel/pulp rtio ing vegetbles, cucurbits - edible peel Cucumber nd courgette (summer sqush) A totl of eight supervised trils were conducted on protected cucumber in Europe (Frnce, Germny, Itly, Spin nd the UK) in nd. These trils support the registered indoor GAP in the UK (i.e. 5 g i/h; dy PHI). Smples of cucumber fruits were collected on the sme dy of ppliction immeditely fter the spry deposit hd dried nd pproximtely, nd dys fter the finl ppliction. Smples were immeditely frozen nd mintined in frozen storge for periods of up to months prior to extrction. Residues of isopyrzm (both isomers) in cucumber were determined using nlyticl method GRM6.B with n LOQ of.5 mg/kg for ech isomer (. mg/kg for the sum of both isomers). Residues of CSCD in cucumber were determined using nlyticl method GRM6.A with n LOQ of.5 mg/kg. The results of the trils re summrised in Tble 6 Tble 6 Residues in Cucumber (indoor trils) from supervised trils in Europe involving folir pplictions of isopyrzm (5 g/l EC formultion) Cucumber Appliction DALA Residues Tril Loction Country, yer (Vriety) Rte (g i/h) Wter (L/h) No. (dys) CSCD Totl b Tril: S-55- Regine Kuchly, Frnce, (Verdon) + dy Report : S-55 Study : S-55 Tril: S-55- Snlúcr de Brrmed, Spin, (Llno Verde) dy <.5 Report : S-55 S-55

8 688 Cucumber Appliction DALA Residues Tril Loction Country, yer (Vriety) Rte (g i/h) Wter (L/h) No. (dys) CSCD Totl b Tril: S-645- Cottinghm, UK, (Bon Bon) 6 + dy 4. <. <. < <.5 Report : S-645 Study : S-645 Tril: S-645- Vetschu, Brndenburg, Germny, (Indir) 9 + dy Report :S-645 Study :-645 Tril: S-645- Longué-Jumelles, Frnce, (Verdon) 5 + dy < <.5 Report : S Tril: S-646- Fondi, Itly, (Ekron) dy <. < <.5 Report : S-646 Study : S-646 Tril: S-646- Andlucí, Spin, (Alnis) dy <. < <.5 Report : S-646 Study : S-64 Tril: S-646- Andlucí, Spin, (Llnero) 9 + dy <. < <.5 Report : S-646 Study : S-646 Sum of syn- nd nti-isomer (i.e. residue definition for enforcement) b Sum of isopyrzm nd CSCD expressed s isopyrzm (i.e. residue definition for risk ssessment) ing vegetbles, cucurbits - inedible peel Melon nd wtermelon A totl of eight supervised trils were conducted on protected melon in Europe (Frnce, Spin nd Itly) in nd. These trils support the registered indoor GAP in the UK (i.e. 5 g i/h; dy PHI). Smples of melon whole fruits were collected on the sme dy of ppliction immeditely fter the spry deposit hd dried nd pproximtely, nd dys fter the finl ppliction. Smples were immeditely frozen nd mintined in frozen storge for periods of up to 5 months prior to extrction. Residues of isopyrzm (both isomers) in melon were determined using nlyticl method GRM6.B with n LOQ of.5 mg/kg for ech isomer (. mg/kg for the sum of both isomers). Residues of CSCD in melon were determined using nlyticl method GRM6.A with n LOQ of.5 mg/kg. The results of the trils re summrised in Tble 8.

9 689 Tble 8 Residues in melon (indoor) from supervised trils in Europe involving folir pplictions of (6.5 g/l or 5 g/l EC formultion) Melon Appliction DALA Tril Loction Country, yer (Vriety) Rte (g i/h) Wter (L/h) No. (dys) Residues CSCD Totl b Tril: S- 55- Epieds, Frnce, (Ugo) Tril: S- 64- Stotzheim, Frnce, (Snton) Tril: S- 64- Orgerus, Frnce, (Edgr) Tril: S- 64- Epieds, Frnce, (Hugo) dys + 6 dys + 4 dys dys Peel Pulp Peel Pulp Peel Pulp Peel Pulp Peel Pulp Peel Pulp Peel Pulp Peel Pulp Peel Pulp Peel Pulp Peel Pulp Peel Pulp Peel Pulp Peel Pulp Peel Pulp Peel Pulp.65 <...9 < <..9.5 <...5 <... <..5.4 <..9. <...5 <..5.8 <..6.4 <...9 <...4 <..4.4 <...4 <... < < < < < < < < < <.5..4 <.5..9 < < < <.5..9 <.5.. <.5.9 S-55 S-55 S-64 S-64 S-64 S-64 S-64 S-64

10 69 Melon Appliction DALA Residues Tril Loction Country, yer (Vriety) Rte (g i/h) Wter (L/h) No. (dys) CSCD Totl b Tril: S Snlúcr de Brrmed, Spin, (Prim) + dys 8 Peel Pulp Peel Pulp Peel Pulp Peel Pulp.6 < < < <... < < < <.5. S-554 S-554 Tril: S Cstelsrrsin, Frnce, (Hugo) dys Peel Pulp Peel Pulp Peel Pulp Peel Pulp.8 <...6 <...4 <..5.9 <...8 < < <.5..4 <.5.8 S-648 S-648 Tril: S Mudison, Frnce, (Cisco) 5 + dys 9 Peel Pulp Peel Pulp Peel Pulp Peel Pulp.46 < <..49. < < < < <.5.5. <.5. S-648 S-648 Tril: S Sermide, Itly, (Honey Moon) 5 + dys interv 5 Peel Pulp Peel Pulp Peel Pulp Peel Pulp.49 < <..9.6 < <...54 < < <.5..6 < S-648 Sum of syn- nd nti-isomer (i.e. residue definition for enforcement) b Sum of isopyrzm nd CSCD expressed s isopyrzm (i.e. residue definition for risk ssessment) c Residues clculted using the peel/pulp rtio

11 69 ing vegetbles, other thn Cucurbits Pepper (sweet nd chili) A totl of eight supervised trils were conducted on protected pepper in Europe (UK, Frnce, Itly nd Spin) in nd. These trils support the registered indoor GAP in the UK (i.e. 5 g i/h; dy PHI). Smples of pepper fruits were collected on the sme dy of ppliction immeditely fter the spry deposit hd dried nd pproximtely, nd dys fter the finl ppliction. Smples were immeditely frozen nd mintined in frozen storge for periods of up to 6 months prior to extrction. Residues of isopyrzm (both isomers) hve been found to be stble during frozen storge in wide rnge of crops stored t bout -8 C, during storge periods over 4 months. Residues of isopyrzm (both isomers) in pepper were determined using nlyticl method GRM6.B with n LOQ of.5 mg/kg for ech isomer (. mg/kg for the sum of both isomers). Residues of CSCD in pepper were determined using nlyticl method GRM6.A with n LOQ of.5 mg/kg. The results of the trils re summrised in Tble 9. Tble 9 Residues in pepper (indoor) from supervised trils in Europe involving folir pplictions of (5 g/l or 6.5 g/l EC formultion) Pepper Appliction DALA Tril Loction Country, yer (Vriety) Tril: S-649- Wlthm Abbey, UK, (Soprno) Tril: S-649- St Pryvé St Mesmin, Frnce, (Sprtkus) Tril: S-649- Orgerus, Frnce, (Lipri) Tril: S Vrennes Sur Loire, Frnce, (Denver) Tril: S-558- Fondi, Itly, (Peppone) Tril: S-65- Fondi, Itly, (Corrdo) Tril: S-65- Vllemrin, Itly, (Senior) Tril: S-65- Ige Mrin, Itly, (Roni) Rte (g i/h) dy + dy + dy + dy + 8 dy + dy dy 4 + dy Wter (L/h) Residues No. (dys) CSCD Sum of syn- nd nti-isomer (i.e. residue definition for enforcement) <. <. < Totl b <.5 <.5 < b Sum of isopyrzm nd CSCD expressed s isopyrzm (i.e. residue definition for risk ssessment) S-649 S-649 S-649 S-649 S-649 S-649 S-649 S-649 S-558 S-558 S-65 S-65 S-65 S-65 S-65 S-65

12 69 Tomto nd ubergine (egg plnt) A totl of eight supervised trils were conducted on protected tomto in Europe (Frnce, Spin, UK nd Itly) in, nd. These trils support the registered indoor GAP in the UK (i.e. 5 g i/h; dy PHI). Smples of tomto fruits were collected on the sme dy of ppliction immeditely fter the spry deposit hd dried nd pproximtely, nd dys fter the finl ppliction. Smples were immeditely frozen nd mintined in frozen storge for periods of up to 4 months prior to extrction. Residues of isopyrzm (both isomers) in tomto were determined using nlyticl method GRM6.B with n LOQ of.5 mg/kg for ech isomer (. mg/kg for the sum of both isomers). Residues of CSCD in tomto were determined using nlyticl method GRM6.A with n LOQ of.5 mg/kg. The results of the trils re summrised in Tble. Tble Residues in tomto (indoor) from supervised trils in Europe involving folir pplictions of (5g/L EC formultion). Tomto Appliction DALA Tril Loction Country, yer (Vriety) Rte (g i/h) Tril: S Dmpierre en burly Loiret, Frnce, dys (Lucind) Tril: S-556- Cnls, Vlenci Spin, (Grnillon) Tril: S-65- Dmpierre en burly, Loiret, Frnce, (Lucind) Tril: S-65-4 Newbourne, Suffolk, UK, (Conchit) Tril: S-65- Elne,Pyrenees-Orientles, Lnguedoc-Roussillon, Frnce, (Sweet) dys dys 9 + dys + dys interv 5 + Tril: S-65- Vllemrin,Ltin, Lzio, dys Itly, (Tropicl) interv Tril: S-65- Conil de l Fronter,Cdiz, Andluci, Spin, (Lupit) Tril: S-54- Ltin Itly, (Tropicl) dys interv + dys interv Wter (L/h) Residues No. (dys) CSCD Sum of syn- nd nti-isomer (i.e. residue definition for enforcement) < Totl b < b Sum of isopyrzm nd CSCD expressed s isopyrzm (i.e. residue definition for risk ssessment) S-555 S-555 S-556 S-556 S-65 S-65 S-65 S-65 S-65 S-65 S-65 S-65 S-65 S-65 S-54 S-54

13 69 nd tuber vegetbles Crrot A totl of sixteen supervised trils were conducted on crrot in Europe (UK, Germny, Frnce, Spin nd Itly) in nd. These trils support the registered GAP in the UK (i.e. 5 g i/h; 4 dy PHI). Smples of crrot roots were collected on the sme dy of ppliction immeditely fter the spry deposit hd dried nd pproximtely,, nd 4 dys fter the finl ppliction. Smples were immeditely frozen nd mintined in frozen storge for periods of up to 6 months prior to extrction. Residues of isopyrzm (both isomers) in crrot were determined using nlyticl method GRM6.B with n LOQ of.5 mg/kg for ech isomer (. mg/kg for the sum of both isomers). Residues of CSCD in crrot were determined using nlyticl method GRM6.A with n LOQ of.5 mg/kg. The results of the trils re summrised in Tble. Tble Residues in crrots (outdoor) from supervised trils in Europe involving folir pplictions of (6.5 g/l or 5 g/l EC formultion). Crrots Appliction DALA Tril Loction Country, yer (Vriety) Tril: S-549- Rinford Lncshire, UK, (Artist) Tril: S-549- Kutenholz Niederschsen, Germny, (Vito) Tril: S-64- Shenton, Stffordshire, UK, (Nirobi) Tril: S-64- Rinford, Lncshire, North West, UK, (Nigel) Tril: S-64- Dreetz, Brndenburg, Germny, (Job) Tril: S-64-4 Kutenholz, Niederschsen, Germny, (Nntise DP44) Tril: S-64-5 Niederni, Alsce, Frnce, (Texto) Rte (g i/h) dy dy dy dy 5+ 9 dy dy dy Wter (L/h) No. (dys) Residues <. < <. CSCD Totl b S- 549 S- 549 S- 549 S- 549 S- 64 S- 64 S- 64 S S-. 64 <.5 S- < S- 64 S S S <.5

14 694 Crrots Appliction DALA Residues Tril Loction Country, yer (Vriety) Rte (g i/h) Wter (L/h) No. (dys) CSCD Totl b Tril: S-64-6 Allonnes, Anjou, Pys de loire, Frnce, (Mondibel F) 4+ 4 dy <..9 S S-. 64 <.5 Tril: S-55- Argeles surmer, Pyrenees-Orientl, Lnguedoc-Roussillon, Frnce, (Chmbord) dy S- 55 S- 55 Tril: S-55- Conil de l Fronter, Cdiz, Andluci, Spin, (Nevi) + 4 dy <. <. <.. <. <.5 S- <.5 55 <.5 S <.5 Tril: S Blgnc, Midi Pyrenees, 8 Frnce, 4 dy (Mestro) <..9 S S <.5 Tril: S-644- Argeles sur Mer, Pyrenees-Orientles, Frnce, (Chmbord) dy 96 4 < < S- 644 S- 644 Tril: S-644- Ferrr, Emili Romgn, Itly, (Romnce) dy < < S- 644 S- 644 Tril: S Rovigo, Veneto, Itly, (Bolero) 4+ 4 dy <..99. S S- < Tril: S Andluci, Cdiz, Spin, (Nevir) 8+ 4 dy S- 644 S- 644 Tril: S Andluci, Cdiz, Spin, (Nevir F) dy S- 644 S- 644 Sum of syn- nd nti-isomer (i.e. residue definition for enforcement) b Sum of isopyrzm nd CSCD expressed s isopyrzm (i.e. residue definition for risk ssessment) Cerel grins Brley A totl of eighteen supervised trils were conducted on brley in Europe (Frnce, Germny, Itly, Spin, Switzerlnd nd the UK) in 6 nd. These trils support the registered GAP in the UK

15 695 (i.e. 5 g i/h; BBCH < 6). Smples of brley whole plnts were collected on the dy of ppliction immeditely fter the spry deposit hd dried nd lso t further immture stges. Immeditely before mturity in some trils, plnts were smpled nd seprted into immture grin nd the remining plnt. Finlly, smples of grin nd strw were tken t mturity. Smples were immeditely frozen nd mintined in frozen storge for periods of up to months prior to extrction. Residues of isopyrzm (both isomers) in brley were determined using nlyticl method GRM6.B with n LOQ of.5 mg/kg for ech isomer (. mg/kg for the sum of both isomers). Residues of CSCD in brley were determined using nlyticl method GRM6.A with n LOQ of.5 mg/kg. The results of the trils re summrised in Tble Error! Reference source not found., where residues relevnt to risk ssessment nd the setting of MRLs for brley commodities re underlined. Tble Residues in brley (outdoor trils) from supervised trils in Northern Europe involving folir pplictions of (5g/L EC formultion) Brley Appliction DALA Tril Loction Country, yer (Vriety) Tril: CH-FR-6- CH-896 Vouvry, Switzerlnd, 6( Bologn ) Tril: CH-FR-6-4 CH-88 Bex, Switzerlnd, 6 ( Merlot ) Tril: FR-FR BEINE NAUROY, Frnce, 6 ( Esterel ) Tril: IT-FR-6- Rubno, Itly, 6( Borele ) Rte (g i/h) + 6 dys + 6 4dys dys 6 + dys dys dys + 9 4dys dys 4 + 4dys + 5 5dys Wter (L/h) Residues No. (dys) CSCD Totl b 54 Grin <. <.5 48 Grin Grin Grin <. <.5 48 Grin Grin Grin <. <.5 54 Grin Grin Grin <. <.5 CEMR-9- T64-6 CEMR-9- T64-6 CEMR- 9- T64-6 CEMR-94- T65-6

16 696 Brley Appliction DALA Tril Loction Country, yer (Vriety) Tril: ES-FR-6-8 L Overuel, Spin, 6( Verticle ) Tril: FR-FR BEDARRIDES "Les Tilldes", Frnce, 6 ( Brk ) Tril: FR-FR-6-8 GRISOLLES "Les crespys, Frnce, 6 (Nevd) Tril: DE-FR-6-44 Stephnsposching, Germny, 6 (Crt) Tril: AF/59/SY/ Alper, Spin, (Chmorro) Tril: AF/59/SY/ Pilstri,, Spin, (Sonor) Tril: AF/59/SY/ St Etienne sur Reyssouze, Frnce, ( Scrlett ) Tril: AF/59/SY/4 Cordes Frnce, ( Prestige ) Rte (g i/h) + dys dys + dys + 9 dys + dys 6 + 5dys + dys 4+ 4 dys dys dys + 5 5dys 6 + 9dys dys interv + 4 dys interv Wter (L/h) Residues No. (dys) CSCD Totl b 45 Grin Grin Grin Grin Grin Grin..6 4 Grin Grin Immture Grin Grin <.<..6 < Grin <. <.5 45 Grin Grin Grin Grin Grin Immture grin Grin Grin CEMR-94- T65-6 CEMR-9- T64-6 CEMR-9- T64-6 T88-6- T88-6 CEMR-65 - CEMR-65 - CEMR-65 - Version CEMR-65 - Version

17 69 Brley Appliction DALA Tril Loction Country, yer (Vriety) Tril: AF/59/SY/5 Vzerc, Frnce, ( Pltine ) Tril: AF/58/SY/ Perouse, Germny, ( Bremr ) Tril: AF/58/SY/ Heidelberg, Germny, ( Bremr ) Rte (g i/h) dys interv 9 Wter (L/h) Residues No. (dys) CSCD Totl b 8 6 Grin < Grin... Immture Grin Grin CEMR-65 - Version CEMR-6 Version CEMR-6 Version Tril: AF/58/SY/ Arnold, UK, Westmi-nster) Grin.6.6. CEMR-6 Version Tril: AF/58/SY/4 Loiret, Frnce, (Colibri) Immture Grin Grin CEMR-6 Version Tril: AF/58/SY/5 Sone et Loire, Frnce, (Pltine) Immture Grin Grin CEMR-6 Version Sum of syn- nd nti-isomer (i.e. residue definition for enforcement) b Sum of isopyrzm nd CSCD expressed s isopyrzm (i.e. residue definition for risk ssessment) Whet, rye nd triticle A totl of eighteen supervised trils were conducted on whet in Europe (Frnce, Germny, Itly, Spin nd the UK) in 6 nd. These trils support the registered GAP/lbel (± 5%) in the UK (i.e. 5 g i/h; BBCH < ). Smples of whet whole plnts were collected on the sme dy of ppliction immeditely fter the spry deposit hd dried nd lso t further immture stges. Immeditely before mturity in some trils plnts were smpled nd seprted into immture grin nd the remining plnt. Finlly, smples of grin nd strw were tken t mturity. Smples were immeditely frozen nd mintined in frozen storge for periods of up to 5 months prior to extrction. Residues of isopyrzm (both isomers) in whet were determined using nlyticl methods GRM6.A nd GRM6.B with n LOQ of.5 mg/kg for ech isomer (. mg/kg for the sum of both isomers). Residues of CSCD in whet were determined using nlyticl method GRM6.A with n LOQ of.5 mg/kg. The results of the trils re summrised in Tble, where residues relevnt to risk ssessment nd the setting of MRLs for whet commodities re underlined.

18 698 Tble Residues in whet (outdoor trils) from supervised trils in Spin, Itly nd Southern Frnce involving folir pplictions of (5g/L EC formultion) Whet Appliction DALA Tril Loction Country, yer (Vriety) Tril: FR-FR-6-, 85 MALPART "L Pointe", Frnce,6 (Limoner) Tril: FR-FR-6-, 85 GRIVESNES "Le Moulin", Frnce,6 (Limes) Tril: FR-FR-6-4, 549 BEINE NAUROY "Menu Dime ", Frnce,6 (Chrger) Rte (g i/h) + 4dys dys+dys dys+dys dys dys+ dys dys+ dys + 4 dys dys+ 6dys dys+ dys Wter (L/h) No. (dys) Grin Immture grin Grin Immture grin Grin Residues CSCD Totl b <. <.5 CEMR Grin Grin Grin CEMR CEMR-9- CEMR-9- CEMR-9- CEMR-9-6 Grin <. <.5 CEMR-9-4 Grin <. <.5 CEMR-9-4 Grin <. <.5 CEMR-9- Tril: ES-FR-6-5, L Overuel, Spin,6 (Fiuf) 4+ 4dys + + 4dys+ dys Grin Immture grin Grin CEMR-9- CEMR-9- Tril: ES-FR-6-6, Wmb, Spin,6 (Soissons) + 5 6dys 5 Grin <. <.5 CEMR-9-

19 699 Whet Appliction DALA Tril Loction Country, yer (Vriety) Tril: FR-FR-6-, 8 GRISOLLES "Mistrl" S9, Frnce,6 (Aztec) Rte (g i/h) dys+ 4dys 9+ 5dys dys+ 8ys Wter (L/h) No. (dys) Grin Grin Residues..8 CSCD Totl b.8.6 CEMR-9-6 Grin <. <.5 CEMR-9-4 Grin CEMR-9- Tril: FR-FR-6-8, 459 MARSILLARGUES "Sole ", Frnce,6 (Aztec) + dys dys+ 6dys Grin Grin.8. CEMR-9- CEMR-9- Tril: DE-FR-6-44, Stetten, Germny,6 (Dekn) Tril: AF/5/SY/, Ayor, Spin, (Otir) Tril: AF/5/SY/, Cstgnolino, Itly, (Bologn) Tril: AF/5/SY/, Budrio, Itly, (Duilio) Tril: AF/5/SY/4, Brry d Islemde, Frnce,(Qulity) dys dys+ 9dys dys+ 9dys dys+ 8dys dys+ 6dys + 5+ dys+ dys + + dys+ dys Grin <. <.5 T Grin Grin Grin Grin Grin Grin. <.. < Grin Grin Grin Grin <. <.5.5 <.5 T <.5 T Report : CEMR-64 Version Report : CEMR-64 Version.8.6 Report : CEMR-64 Version Report : CEMR-6 Version

20 Whet Appliction DALA Tril Loction Country, yer (Vriety) Tril: AF/5/SY/5, Verdun sur Gronne, Frnce,(Prr58) Rte (g i/h) dys+ 8dys + Tril: AF/5/SY/, 5+ Dollern Lower Sxony, Germny,(Hermn) 4dys+ dys Tril: AF/5/SY/, Burweg, Lower Sxony, Germny,(Atlntis) Tril: AF/5/SY/, Meshm, UK, ( Mlcc) Tril: AF/5/SY/4, Rouvres Sint Jen, Frnce, (Courtot) dys+ 5dys dys+ dys dys+ dys Wter (L/h) No. (dys) Immture grin Grin Residues.4 <. CSCD Totl b.95 <.5 Report :CEMR- 6 Version 4 Grin <. <.5 CEMR-66 - Version 4 Grin <. <.5 CEMR-66 - Version 4 Grin Immture Grin Immture Grin Grin <. <. <. CEMR-66 - Version <.5 <.5 <.5 CEMR-66 - Version Tril: AF/5/SY/5, Mrtilly les Brncion, Frnce, (Soissons) dys+ dys Immture Grin Grin <. <. <.5 <.5 CEMR-66 - Version Sum of syn- nd nti-isomer (i.e. residue definition for enforcement) b Sum of isopyrzm nd CSCD expressed s isopyrzm (i.e. residue definition for risk ssessment) Oilseeds Rpe seed (cnol) A totl of twenty supervised trils were conducted on oilseed rpe in Europe (Frnce, Germny, Spin, Itly nd the UK) in 9,, 4 nd 6. Eight trils were conducted t 5 g i/h or t n exggerted rte of 5 g i/h t BBCH Smples of oilseed rpe whole plnts were collected on the sme dy of ppliction immeditely fter the spry deposit hd dried nd pproximtely, 4, 8 nd 4 dys fter the finl ppliction for, nd 4 trils, nd pproximtely,,, nd 4 dys fter the finl ppliction for 6 trils. Smples of oilseed rpe seeds were collected t norml commercil hrvest (4 dys fter the finl ppliction). Smples were immeditely frozen nd mintined in frozen storge for periods of up to months prior to extrction. Residues of isopyrzm (both isomers) in oilseed rpe (whole plnts nd seeds) were determined using nlyticl method GRM6.B with n LOQ of.5 mg/kg for ech isomer (. mg/kg for the sum of both isomers). Residues of CSCD in oilseed rpe (whole plnts nd seeds) were determined using nlyticl method GRM6.A with n LOQ of.5 mg/kg. The results of the trils re summrised in Tble 4, where residues relevnt to risk ssessment nd the setting of n MRL for oilseed rpe re underlined

21 Tble 4 Residues in Oilseed rpe (outdoor) from supervised trils in Europe involving folir pplictions of (5 g/l SC formultion) Oilseed rpe Appliction DALA Tril Loction Country, yer (Vriety) Tril: S9-59- Drusenheim, Alsce, Frnce, 9 (Ability) Tril: S9-59- Knottingley Yorkshire UK WF DQ, UK, 9 (Morley) Tril: S9-59- Brodstone Lne Ticknll, Derbyshire DE 8LD, UK, 9 (Heros) Tril: S Helmster Weg, Dollern, Lower Sxony, Germny,9 (Ability) Tril: S-96- Normnton On Sor, Leicestershire, UK, (Exclibur) Tril: S-96- Upton Wrwickshire, UK, (dk cbernet) Tril: S-96- Blumberg, Brnim, Brndenburg, Germny, (Titn Rw) Tril: S-96-4 Leouvile, Loiret, Frnce, (Sfrn) Tril: S-4- Trn et Gronne, Midi Pyrennees, Frnce, 4 (Chrome) Rte (g i/h) dy dy dy dy dy dy + 6 dy dy dy Wter (L/h) No. (dys) Seed Seed Seed Seed Seed Seed Seed Seed Residues CSCD Totl b Seed <..4. S9-59- S9-59 S9-59- S9-59 S9-59- S9-59 S9-59- S9-59 S- 96- S- 96 S- 96- S- 96 S- 96- S- 96 S- 96- S- 96 S- 4 S- 4

22 Oilseed rpe Appliction DALA Tril Loction Country, yer (Vriety) Tril: S-4- Trn et Gronne, Midi Pyrennees, Frnce, 4 (Btzz) Tril: S-4- Argon, Zrgoz, Spin, 4 (Fricol) Tril: S-4-4 Bologn, Itly, 4 (Primus) Tril: S5-94- North Yorkshire, Yorkshire, UK, 5 (Compss) Tril: S5-94- Leicestershire, Leicestershire, UK, 5 (DK Cbernet) Rte (g i/h) + 8 dy dy + dy Wter (L/h) No. (dys) Residues CSCD Totl b 64 Seed Seed < Seed Seed Seed S- 4 S- 4 S- 4 S- 4 S- 4 S- 4 S5-94 S5-94 S5-94 S5-94 Tril: S5-94- Leicestershire, Leicestershire, UK, 5 (DK Cmelot) 9 9 Seed S5-94 S5-94 Tril: S Brnim, Brndenburg, Germny,5 (Flyer) Tril: S5-94- Bologn, Emili Romgn, Itly, 5 (Exclibur) Tril: S5-94- Herult, Lnguedoc- Roussillon, Frnce, 5 (Attletick) Tril: S5-94- Trn Et Gronne, Midi Pyrenees, Frnce, 5 (Expertise) Seed Seed Remining plnt Seed Seed < < S5-94 S5-94 S5-94 S5-94 S5-94 S5-94 S5-94 S5-94

23 Oilseed rpe Appliction DALA Residues Tril Loction Country, yer (Vriety) Rte (g i/h) Wter (L/h) No. (dys) CSCD Totl b Tril: S Huesc, Argon, Spin, 5 (Expertise) Seed Remining plnt S5-94 S5-94 Sum of syn- nd nti-isomer (i.e. residue definition for enforcement) b Sum of isopyrzm nd CSCD expressed s isopyrzm (i.e. residue definition for risk ssessment) Penut A totl of four supervised trils were conducted on penut in Ltin Americ (Nicrgu) in. Trils were conducted t n exggerted/overdosed rte ( 5 g i/h per crop cycle) compred to the registered criticl GAP/lbel rte ( 5 g i/h per crop cycle). Smples of penut (nutmet) were collected on the sme dy of ppliction immeditely fter the spry deposit hd dried nd pproximtely,, nd 4 dys fter the finl ppliction. Smples were immeditely frozen nd mintined in frozen storge for periods of up to months prior to extrction. Residues of isopyrzm (both isomers) in penut were determined using nlyticl method GRM6.B with n LOQ of.5 mg/kg for ech isomer (. mg/kg for the sum of both isomers). Residues of CSCD in penut were determined using nlyticl method GRM6.A with n LOQ of.5 mg/kg. The results of the trils re summrised in Tble 5, where residues relevnt to risk ssessment nd the setting of n MRL for penut re underlined Tble 5 Residues in Penut (outdoor trils) from supervised trils in Nicrgu involving folir pplictions of (5 g/l EC formultion) Penut Appliction DALA Residues Tril Loction Country, yer (Vriety) Rte (g i/h) Wter (L/h) No. (dys) CSCD Totl b Tril: TK466- Cofrdi, Nicrgu, (Georgi Green) dy Nutmet <. <.5 TK466 TK466 Tril: TK466- Cofrdi, Nicrgu, (Georgi Green) dy Nutmet Nutmet Nutmet Nutmet Nutmet <. <. <. <. <. <.5 <.5 TK466 <.5 <.5 TK466 <.5 Tril: TK466-4 Cofrdi, Nicrgu, (Georgi Green) dy Nutmet Nutmet Nutmet Nutmet Nutmet <. <. <. <. <. <.5 <.5 TK466 <.5 <.5 TK466 <.5 Tril: TK466-5 Cofrdi, Nicrgu, (Georgi Green) dy Nutmet <. <.5 TK466 TK466 Sum of syn- nd nti-isomer (i.e. residue definition for enforcement) b Sum of isopyrzm nd CSCD expressed s isopyrzm (i.e. residue definition for risk ssessment)

24 4 Primry niml feed commodities Forge, nd fodder (dry) of cerels Tble 6 Residues in brley plnt nd strw (outdoor trils) from supervised trils in Northern Europe involving folir pplictions of (5g/L EC formultion) Brley Appliction DALA Tril Loction Country, yer (Vriety) Tril: CH-FR-6- CH-896 Vouvry, Switzerlnd, 6( Bologn ) Tril: CH-FR-6-4 CH-88 Bex, Switzerlnd, 6 ( Merlot ) Tril: FR-FR BEINE NAUROY, Frnce, 6 Rte (g i/h) + 6 dys + 6 4dys dys 6 + dys dys dys + 9 4dys Wter (L/h) No. (dys) Residues CSCD Totl b CEMR-9- T64-6 CEMR-9- T64-6 CEMR-9- T64-6 CEMR-9- T64-6 CEMR-9- T64-6 CEMR-9- T64-6 CEMR- 9- T64-6

25 5 Brley Appliction DALA Tril Loction Country, yer (Vriety) Rte (g i/h) ( Esterel ) dys 4 + 4dys + 5 5dys Tril: IT-FR-6- Rubno, Itly, 6( Borele ) + dys Tril: ES-FR-6-8 L Overuel, Spin, 6( Verticle ) Tril: FR-FR BEDARRIDES "Les Tilldes", Frnce, 6 ( ) Brk Tril: FR-FR-6-8 GRISOLLES "Les crespys, Frnce, 6 (Nevd) Tril: DE-FR-6-44 Stephnsposching, Germny, dys + dys + 9 dys + dys 6 + 5dys + dys Wter (L/h) No. (dys) dys Er Immture Er Immture Remining plnt Remining plnt Residues CSCD Totl b Immture CEMR-9- T64-6 CEMR-9- T64-6 CEMR-94- T65-6 CEMR-94- T65-6 CEMR-94- T65-6 CEMR-94- T65-6 CEMR-9- T64-6 CEMR-9- T64-6 CEMR-9- T64-6 CEMR-9- T64-6 T88-6- T88-6

26 6 Brley Appliction DALA Tril Loction Country, yer (Vriety) (Crt) Tril: AF/59/SY/ Alper, Spin, (Chmorro) Tril: AF/59/SY/ Pilstri,, Spin, (Sonor) Tril: AF/59/SY/ St Etienne sur Reyssouze, Frnce, ( Scrlett ) Tril: AF/59/SY/4 Cordes Frnce, ( Prestige ) Tril: AF/59/SY/5 Vzerc, Frnce, ( ) Pltine Tril: AF/58/SY/ Perouse, Germny, ( Bremr ) Rte (g i/h) dys dys dys 6 + 9dys dys dys dys 99 9 Wter (L/h) No. (dys) Residues CSCD Totl b Remining plnt Er Remining plnt < T88-6- T88-6 T88-6- T88-6 CEMR-65 - Version Study :CEMS-65 CEMR-65 - Version CEMS-65 CEMR-65 - Version CEMS-65 CEMR-65 - Version CEMS-65 CEMR-65 - Version CEMS-65 CEMR-6 Version CEMS-6 Tril: AF/58/SY/ Heidelberg, Germny, ( Bremr ) - 8 Remining Plnt CEMR-6 Version CEMS-6

27 Brley Appliction DALA Residues Tril Loction Country, yer (Vriety) Rte (g i/h) Wter (L/h) No. (dys) CSCD Totl b Tril: AF/58/SY/ Arnold, UK, Westminster) CEMR-6 Version CEMS-6 Tril: AF/58/SY/4 Loiret, Frnce, (Colibri) Remining Plnt CEMR-6 Version CEMS-6 Tril: AF/58/SY/5 Sone et Loire, Frnce, (Pltine) Remining Plnt CEMR-6 Version CEMS-6 Sum of syn- nd nti-isomer (i.e. residue definition for enforcement) b Sum of isopyrzm nd CSCD expressed s isopyrzm (i.e. residue definition for risk ssessment) Tble Residues in whet plnt nd strw (outdoor trils) from supervised trils in Spin, Itly nd Southern Frnce involving folir pplictions of (5g/L EC formultion). Whet Appliction DALA Residues Tril Loction Country, yer (Vriety) Rte (g i/h) Wter (L/h) No. (dys) CSCD Totl b Tril: FR-FR-6-, 85 MALPART "L Pointe", Frnce,6 (Limoner) + 4dys dys+dys Immture Grin Remining Plnt CEMR-9- T6-6 (EC) CEMR-9- T6-6

28 8 Whet Appliction DALA Tril Loction Country, yer (Vriety) Tril: FR-FR-6-, 85 GRIVESNES "Le Moulin", Frnce,6 (Limes) Tril: FR-FR-6-4, 549 BEINE NAUROY "Menu Dime ", Frnce,6 (Chrger) Tril: ES-FR-6-5, L Overuel, Spin,6 (Fiuf) Rte (g i/h) Wter (L/h) dys+dys dys dys+ dys dys+ dys + 4 dys dys+ 6dys dys+ dys 4+ 4dys + + 4dys+ dys 5 9 No. (dys) - 5 Immture Grin Remining Plnt Residues CSCD Totl b Remining plnt CEMR-9- T6-6 CEMR-9- T6-6 CEMR-9- T6-6 CEMR-9- T6-6 CEMR-9- T6-6 CEMR-9- T6-6 CEMR-9- T6-6 CEMR-9- T6-6 CEMR-9- T6-6

29 9 Whet Appliction DALA Tril Loction Country, yer (Vriety) Tril: ES-FR-6-6, Wmb, Spin,6 (Soissons) Tril: FR-FR-6-, 8 GRISOLLES "Mistrl" S9, Frnce,6 (Aztec) Tril: FR-FR-6-8, 459 MARSILLARGUES "Sole ", Frnce,6 (Aztec) Rte (g i/h) Wter (L/h) + 5 6dys dys+ 4dys 9+ 5dys dys+ 8ys + dys dys+ 6dys No. (dys) Remining Plnt Residues < CSCD Totl b CEMR-9- T6-6 CEMR-9- T6-6 CEMR-9- T6-6 CEMR-9- T(EC)6-6 CEMR-9- T6-6 CEMR-9- T6-6 Tril: DE-FR-6-44, Stetten, Germny,6 (Dekn) dys dys+ 9dys dys+ 9dys Remining Plnt Remining Plnt T8-6- T8-6 T8-6- T8-6 T8-6- T8-6

30 Whet Appliction DALA Tril Loction Country, yer (Vriety) Rte (g i/h) Wter (L/h) No. (dys) Residues CSCD Totl b Tril: AF/5/SY/, Ayor, Spin, (Otir) dys+ 8dys Report : CEMR-64 Version CEMS-64 Tril: AF/5/SY/, Cstgnolino, Itly, (Bologn ) Tril: AF/5/SY/, Budrio, Itly, (Duilio) Tril: AF/5/SY/4, Brry d Islemde, Frnce,(Qulity ) Tril: AF/5/SY/5, Verdun sur Gronne, Frnce,(Prr58 ) Tril: AF/5/SY/, Dollern Lower Sxony, Germny,( Hermn) Tril: AF/5/SY/, Burweg, Lower Sxony, Germny,( Atlntis) Tril: AF/5/SY/, Meshm, UK, (Mlcc) dys+ 6dys + 5+ dys+ dys + + dys+ dys dys+ 8dys dys+ dys dys+ 5dys dys+ dys Remining Plnt < < Report : CEMR-64 Version CEMS-64 Report : CEMR-64 Version CEMS-64 Report : CEMR-6 Version CEMS-64 Report :CEMR-6 Version CEMS- 64 CEMR-66 - Version CEMS-66 CEMR-66 - Version CEMS-66 CEMR-66 - Version CEMS-66

31 Whet Appliction DALA Residues Tril Loction Country, yer (Vriety) Rte (g i/h) Wter (L/h) No. (dys) CSCD Totl b Tril: AF/5/SY/4, Rouvres Sint Jen, Frnce, ( Courtot) dys+ dys Remining Plnt Remining Plnt CEMR-66 - Version CEMS-66 Tril: AF/5/SY/5, Mrtilly les Brncion, Frnce, ( Soissons) dys+ dys Remining Plnt CEMR-66 - Version CEMS-66 Sum of syn- nd nti-isomer (i.e. residue definition for enforcement) b Sum of isopyrzm nd CSCD expressed s isopyrzm (i.e. residue definition for risk ssessment) RESIDUES IN PROCESSED FOODS ws evluted by the JMPR in where the processing of residues in brley nd whet ws evluted nd where pplicble processing fctors were estblished. A high-temperture hydrolysis study to investigte the nture of residues in processed commodities ws lso evluted by the JMPR. ws concluded to be hydrolyticlly stble under conditions representtive of psteuristion, bking/brewing/boiling nd sterilistion. The Meeting received informtion on processing studies on crrot, tomto, pple, oilseed rpe hve been conducted. Processing of pples The effect of processing on residues of isopyrzm in pples ws studied (Oppillirt, S., 9, Report No. T964--). Two residue field trils were conducted in southern Frnce during 8. The pples were treted with pplictions of 5 g/l isopyrzm EC t rte of g i/h with sevendy s. Seven dys fter the finl ppliction, smples (~9 kg) of treted nd untreted fruit were hrvested from ech tril nd trnsported t mbient temperture to the processing fcility. In ddition, fruit (~ kg) were frozen for residue nlysis. Apple fruit ws processed into suce, juice, cnned fruit nd dried fruit. Suce Approximtely 6 kg of treted fruit ws used for processing to suce: ws thoroughly wshed with wter nd strined. Wshed fruit ws blnched in boiling wter for minutes to void enzymtic browning. Blnched fruit ws crushed with n electric crusher nd sieved to seprte the puree from the peel nd pips. The Brix degree of the rw puree ws mesured, sugr dded nd the puree ws reduced by heting in double jcket sucepn to obtin Brix degree of 4%. The ph of the suce ws mesured, the suce ws pckged into glss jrs, sterilised t 5/ C for minutes nd cooled. Juice: Approximtely 5 kg of treted fruit ws used for processing to juice: ws thoroughly wshed with wter nd strined. Wshed fruit ws crushed with n electric crusher, pressed nd the juice

32 seprted from the pomce. The wet pomce ws dried in n oven t 6 C for two dys. Pectolytic enzymes (. ml/l) were dded to the juice which ws left to settle for t lest hours. The juice ws rcked from the deposits, filtered over trimming pltes using filtrtion unit under nitrogen pressure. The ph of the juice ws mesured nd the juice ws psteurised t 85 C for t lest minute nd cooled. Cnned fruit: Approximtely kg of treted fruit ws used for processing to cnned fruit. ws thoroughly wshed with wter nd strined. Wshed fruit ws peeled nd the peeled fruit blnched in boiling wter for pproximtely two minutes. The blnched fruit ws cored nd cut into pieces. Scchrose syrup (% sugr, ph ~.5) ws dded to the cored fruits t one third syrup to two thirds fruit, nd the mixture seled in cns. Dried fruit: Approximtely kg of treted fruit ws used for processing to dried fruit. ws thoroughly wshed with wter nd strined. Wshed fruit ws cored nd cut into prts, then sliced into mm thick pieces with mndolin. Apple slices were plced on siliconised pper in n oven t 6 C until fully dried nd turned over dily. Two blnce studies nd two follow-up studies were crried out on ech process. At ech processing step sub-smples of the treted nd untreted processed commodities, including wste products, were tken nd frozen. Smples were nlysed for isopyrzm (s SYN54969 nd SYN54968) using method GRM6.B nd for CSCD nd CSCD using method GRM6.A. Mss blnces for isopyrzm residues were clculted for ech process. Processing fctors were clculted for isopyrzm nd for the sum of isopyrzm nd CSCD The isopyrzm residues in pple fruit prior to processing nd suce, juice, cnned fruit nd dried fruit, s well s in the intermedite processed frctions nd the processing by-products from the two blnce studies re presented below. Tble 8 residues in pple nd processed commodities during processing of fruit into suce Blnce S Suce (SYN SYN54969) processing specimen Smple Residue Totl Residue (mg) % Recovered s Proportion of Initil Residue Whole pple fruit prior to processing * Whole fruit sub-specimen * Wsh wter Wshed fruit Blnching wter.5.8 #.4 Blnched fruit Rw suce Wste Reduced fruit..6. Suce Blnce (whole fruit + wsh wter + wshed fruit + blnching wter + blnched fruit + rw suce + wste + reduced fruit + suce) 9 * - Men of two smples for SYN54968 nd SYN # - SYN54969 only; SYN54968 residues <LOQ.

33 Tble9 residues in pple nd processed commodities during processing of fruit into suce Blnce S Suce (SYN SYN54969) processing specimen Smple Residue Totl Residue (mg) % Recovered s Proportion of Initil Residue Whole pple fruit prior to processing * Whole fruit sub-specimen * Wsh wter..54. Wshed fruit Blnching wter Blnched fruit Rw suce...5 Wste Reduced fruit.4.. Suce Blnce (whole fruit + wsh wter + wshed fruit + blnching wter + blnched fruit + rw suce + wste + reduced fruit + suce) * - Men of two smples for SYN54968 nd SYN Tble residues in pple nd processed commodities during processing of fruit into juice Blnce S Suce (SYN SYN54969) processing specimen Smple Residue Totl Residue (mg) % Recovered s Proportion of Initil Residue Whole pple fruit prior to processing * Whole fruit sub-specimen * Wsh wter Wshed fruit Rw juice Wet pomce Dry pomce.44 (.9)** --** Wste Filtered juice.4.9 #. Filter plte.5.. Juice <. -- ##. Blnce (whole fruit + wsh wter + wshed fruit + rw juice + wet pomce + wste + 85 filtered juice + filter plte + juice) * - Men of two smples for SYN54968 nd SYN ** - Dry pomce is formed directly from drying wet pomce nd is not included in the blnce clcultions. # - SYN54969 only; SYN54968 residues <LOQ. ## - Not clculted: SYN54968 nd SYN54969 residues <LOQ. Tble residues in pple nd processed commodities during processing of fruit into juice Blnce S Suce (SYN SYN54969) processing specimen Smple Residue Totl Residue (mg) % Recovered s Proportion of Initil Residue Whole pple fruit prior to processing * Whole fruit sub-specimen * Wsh wter Wshed fruit Rw juice Wet pomce Dry pomce.695 (.554)** --** Wste.4.4. Filtered juice... Filter plte...