Wolbachia Infection in the Coffee Berry Borer (Coleoptera: Scolytidae)
|
|
- Scot Morris Arnold
- 5 years ago
- Views:
Transcription
1 ARTHROPOD BIOLOGY Wolbachia Infection in the Coffee Berry Borer (Coleoptera: Scolytidae) FERNANDO E. VEGA, 1 PABLO BENAVIDES, 2 JEFFREY A. STUART, 2 AND SCOTT L. O NEILL 3 Insect Biocontrol Laboratory, USDAÐARS, Building 011A, BARC-West, Beltsville, MD Ann. Entomol. Soc. Am. 95(3): 374Ð378 (2002) ABSTRACT A nested polymerase chain reaction protocol yielded positive detection of the maternally inherited cytoplasmic proteobacterium Wolbachia in total genomic DNA from coffee berry borers collected in Benin, Brazil, Colombia, Ecuador, El Salvador, Honduras, India, Kenya, Mexico, Nicaragua, and Uganda. Wolbachia was not detected in specimens from Cameroon, the Dominican Republic, Indonesia, Jamaica, and Peru. AmpliÞed bands from India and Brazil were cloned and sequenced. The 438-bp sequence clearly conformed to Wolbachia group B and was nearly identical to that of Ephestia kuehniella. The possible implications of Wolbachia infection in the coffee berry borer are discussed. KEY WORDS Wolbachia, Hypothenemus, coffee berry borer, endosymbionts Mention of trade names or commercial products in this article is solely for the purpose of providing speciþc information and does not imply recommendation or endorsement by the USDA. 1 vegaf@ba.ars.usda.gov. 2 Department of Entomology, Purdue University, 1158 Smith Hall, West Lafayette, IN Department of Zoology and Entomology, University of Queensland, St. Lucia, QLD 4072, Australia. THE COFFEE BERRY BORER, Hypothenemus hampei (Ferrari), is the most important insect pest of coffee throughout the world. Endemic to Central Africa, it has now spread to most coffee growing regions (Baker 1999). Female adults bore a hole in the coffee berry and deposit their eggs; larvae feed on the endosperm, lowering its quality and possibly causing abscission of the berry (Le Pelley 1968). The entire life cycle occurs inside the berry, except when the newly molted adult females emerge in search of another berry to colonize. This cryptic life cycle makes control of this insect extremely difþcult. As is common in some Scolytidae tribes (e.g., Corthylini, Cryphalini, Dryocoetini, Hyorrhynchini, and Xyleborini; Kirkendall 1993), H. hampei (Cryphalini) exhibits a female-biased sex ratio, incestuous intraberry inbreeding (sib-mating), and dwarfed males (Bergamin 1943, Baker 1999). The female-biased sex ratio in Scolytidae is usually ascribed to haplodiploidy, whereby unfertilized eggs result in males, although this has been shown only for the Xyleborini and some members of the Dryocoetini (Entwistle 1964, Kirkendall 1993, Normark et al. 1999). Brun et al. (1995) have reported that male coffee berry borers appear to develop from fertilized eggs and have the same number of chromosomes as females, although only the maternal set of chromosomes is expressed. Another mechanism for skewed sex ratios favoring females has been ascribed to the effects of the maternally inherited intracellular proteobacterium Wolbachia. The mechanism, known as feminization or sex conversionñ whereby genetic males become functional femalesñ was originally reported in isopods (Rousset et al. 1992) and has recently been reported in Ostrinia furnacalis (Guenée) (Kageyama et al. 1998). Wolbachia also causes unidirectional or bidirectional cytoplasmic incompatibility, whereby fertility will depend on the Wolbachia strain present in the insect and on whether the male or female (or both) are infected (OÕNeill et al. 1997; Bourtzis and OÕNeill 1998). Wolbachia has also been shown to induce parthenogenesis in parasitic Hymenoptera (Stouthamer et al. 1990, Zchori- Fein et al. 1992); conßicting reports on parthenogenetic development have been reported for the coffee berry borer (Bergamin 1943, Browne 1961, Entwistle 1964, Muñoz 1989, Barrera et al. 1995). Lastly, Wolbachia has been implicated in male killing, both during early and late developmental stages, in various insects (Hurst et al. 1997, Fialho and Stevens 2000, Hurst et al. 2000, Jiggins et al. 2000). Wolbachia has been detected using the polymerase chain reaction (PCR) in 15Ð20% of insect species sampled (Werren et al. 1995, OÕNeill et al. 1997, Werren 1997, Zhou et al. 1998, Werren and Windsor 2000), although a method known as long-pcr resulted in Wolbachia detection in 76% of species sampled (Jeyaprakash and Hoy 2000). Wolbachia has been detected in several Coleopterans (Wade and Chang 1995, Giordano et al. 1997, Fialho and Stevens 2000, Jeyaprakash and Hoy 2000, Malloch et al. 2000), but only one report of detection in Scolytidae (Ips typographus L.) has been published (Stauffer et al. 1997). We were inter /02/0374Ð0378$02.00/ Entomological Society of America
2 May 2002 VEGA ET AL.: Wolbachia INFECTION IN COFFEE BERRY BORER 375 ested in using molecular techniques to document the presence of Wolbachia in the coffee berry borer to determine whether Wolbachia might be involved in the skewed-sex ratio exhibited by the insect, which would expand knowledge on the basic biology of the insect. Materials and Methods Insects. Coffee berry borers were obtained from infected coffee berries collected in the Þeld in Benin (Niaouli), Brazil (Piracicaba), Cameroon (Atok), Colombia (Santa Rosa de Cabal, Risaralda), Dominican Republic (La Cumbre, Santiago), Ecuador (Via Chone, Santo Domingo, Pichincha), El Salvador (Finca El Espino, Antiguo Cuscatlán, Libertad), Honduras (Yojoa Flora, Peña Blanca, Cortés), India (Elkhill Estate, Siddapur, Kodagu District, Karnataka), Indonesia (Sumber- Asin, Malang), Jamaica (Saint Andrews Parish), Kenya (Coffee Research Foundation in Ruiru, Thika District), Mexico (Finca La Alianza, Chiapas), Nicaragua (km 14, Matagalpa), and Uganda (Muwoko, Mubende District). The extensive coffee berry borer collection from Peru allowed us to analyze males and females separately, but due to the female-biased sex ratio, most of the insects from all other countries were females, and no attempt was made to separate any males, if present. Insects were placed in absolute ethanol while still alive and stored at room temperature until DNA was extracted. PCR Assay. Total genomic DNA was extracted from insects using the DNeasy Tissue Kit (Qiagen, Valencia, CA). Total genomic DNA from Drosophila simulans Sturtevant (Riverside) naturally infected with Wolbachia was used as a positive control. Negative controls consisted of samples lacking DNA template. All experiments were repeated at least twice. A nested PCR protocol was used, with primers designed to amplify the Wolbachia wsp gene fragment (Braig et al. 1998, Zhou et al. 1998): 81F (5 -TGGTC- CAATAAGTGATGAAGAAAC), 691R (5 -AAAAAT- TAAACGCTACTCCA), and 522R (5 -ACCAGCTTT- TGCTTGATA). PCRs were done in 20 l reactions volumes consisting of 1 l DNA template, 17 l Platinum PCR Supermix (Gibco BRL, Gaithersburg, MD), and 1.0 l each of primers 81F and 691R (20pmol/ l). The thermal proþle used in the GeneAmp PCR System 9700 thermocycler (PE Applied Biosystems, Norwalk, CT) was as follows: 94 C for 3 min, followed by 35 cycles of 94 C for 1 min, 55 C for 1 min, and 72 C for 1 min, with a Þnal extension at 72 C for 10min. The PCR product was diluted 1:100 and used for the second PCR with primers 81F and 522R in 20 l reaction volumes as described above. Bands were visualized under UV illumination in a 1% agarose gel stained with ethidium bromide. A 50-bp DNA ladder (Gibco BRL, Gaithersburg, MD, catalog No Ð014) was used to determine the size of the ampliþed DNA band. Before running nested PCR, primers 12SA1 and 12SB1 (speciþc for insect mitochondrial 12S rrna) were used to assess the quality of the DNA (OÕNeill et al. 1992; results not shown). Cloning and Sequencing. DNA bands ( 450bp) from PCR products, corresponding to coffee berry borer specimens from India and Brazil were used for cloning and sequencing. The QIAEX-II Gel Extraction Fig. 1. Wolbachia detection in total genomic coffee berry borer DNA extracted from specimens collected in Brazil (lane 3), Colombia (lane 4), Ecuador (lane 5), India (lane 5), Nicaragua (lane 7), and El Salvador (lane 8). Lane 1 is the DNA ladder (50bp; Gibco BRL), and lane 2 is a positive Wolbachia control from Drosophila. Kit (Qiagen) was used to isolate the DNA fragments from the 1% agarose gel. The TOPO TA Cloning Kit for Sequencing (Invitrogen, Carlsbad, CA) was used for cloning and transformation of the DNA fragments. The solutions containing the DNA fragments and the vector were incubated overnight in prewarmed LB plates with 50 g/ml of kanamycin. Hundreds of colonies were obtained after this procedure. Four colonies from each sample (four Brazilian and four Indian) were selected and cultured overnight in LB medium containing 50 g/ml of kanamycin. To isolate plasmid DNA, the Wizard Plus SV Minipreps DNA PuriÞcation System (Promega, Madison, WI) was used. The DNA fragments were sequenced using an ABI-3700 Automatic Sequencer (PE Applied Biosystems). BLASTX and BLASTN sequence analyses (Altschul et al. 1997) were performed to compare the CBB sequence with other sequences in the GenBank database. Results and Discussion An 450-bp band was obtained from the nested PCR using total genomic DNA from coffee berry borers originating in Benin, Brazil, Colombia, Ecuador, El Salvador, Honduras, India, Mexico, Nicaragua, and Uganda (Fig. 1). The positive Drosophila control, yielded a band of similar size. No ampliþcation was obtained from any of the negative controls (not shown). Cloning and sequencing of four clones from each sample (India and Brazil) revealed a 438-bp sequence (Fig. 2, GenBank Accession AF389084). There were sequence differences between clones, but these were interpreted to be PCR artifacts: one Brazilian clone had a G instead of an A at position 74, and a T instead of an A at position 72. One Indian clone had
3 376 ANNALS OF THE ENTOMOLOGICAL SOCIETY OF AMERICA Vol. 95, no. 3 Fig. 2. Consensus sequence (438 bp) for Wolbachia isolated from Indian and Brazilian coffee berry borers. an A instead of a G at position 149. Interestingly, one Brazilian clone and two Indian clones had a T instead of a C at position 327 (marked by the asterisk in Fig. 2). A T instead ofacatthat position would make this sequence identical to that of the Wolbachia wspb gene isolated from Ephestia kuehniella Zeller (Lepidoptera: Pyralidae) (Sasaki 1999). This sequence clearly indicates that the coffee berry borer Wolbachia belongs to Wolbachia sp. group B (Sasaki 1999). To further investigate the negative ampliþcation in specimens from Cameroon, the Dominican Republic, Indonesia, Jamaica, Kenya, and Peru new primers were designed (pbf2: 5 -TAGCTACTATGTTCGTT- TGC; pbr1: 5 ÐCTCTGTTACTAAAGGATT GC) based on the cloned fragment sequence from Brazil and India. PCR products from nested reactions using primers 81F/691R followed by 81F/522R were used with primers pbf2 and pbr1 to increase sensitivity and yielded positive ampliþcation in all samples that were previously positive as well as in coffee berry borer DNA from Kenya whose ampliþcation had previously been negative. PCR experiments conducted using DNA from samples collected from Cameroon, Dominican Republic, Jamaica, Indonesia, and Peru with primers 81F/691R followed by pbf2/pbr1 were still negative. The lack of ampliþcation in these specimens could be due to the actual absence of Wolbachia in insects from sites sampled in these countries or to a different nucleotide sequence in the Wolbachia DNA which prevents annealing with the primers used. It is also possible that the quality of Wolbachia DNA from these samples might have been compromised, or its titer might have been low. As stated by Jeyaprakash and Hoy (2000), standard PCR techniques frequently produce false negatives, thus requiring more sensitive detection techniques. Our results provide evidence for the presence of Wolbachia in specimens from 11 countries and of infection with a B group Wolbachia in coffee berry borer specimens from India and Brazil. As is the case with other insects, Wolbachia may be playing a signiþcant role in the biology of the coffee berry borer. An interesting phenomenon, termed functional haplodiploidy, has been described for this insect (Brun et al. 1995) which has two related consequences for the paternally derived set of chromosomes in males. First, these chromosomes condense
4 May 2002 VEGA ET AL.: Wolbachia INFECTION IN COFFEE BERRY BORER 377 during spermatogenesis and always fail to be incorporated in the sperm. Second, in males, these chromosomes condense and are never expressed in somatic tissues. Although additional investigations have also reported the elimination of paternal genes in the coffee berry borer (Borsa and Kjellberg 1995), there is no apparent karyotypic difference between male and female coffee berry borers. Thus, it seems reasonable that the cause of this phenomena, as well as the sex determination mechanism itself, may be cytoplasmatic, and may involve Wolbachia. This is, in fact, consistent with recent hypotheses on the mode of action for Wolbachia-induced cytoplasmic incompatibility. These suggest an effect on chromosomes by selectively removing or modifying host proteins involved in chromosomal condensation and de-condensation before and after zygote formation (Bourtzis and OÕNeill 1998, Feder et al. 1999). Furthermore, the work by Peleg and Norris (1972a, 1972b) and Norris and Chu (1980) and Normark et al. (1999) suggests that a maternally inherited bacterium might be involved in sex determination in the scolytid Xyleborus ferrugineus (F.). Past research on coffee berry borer genetics, coupled with the detection of Wolbachia in coffee berry borers from different countries and evidence of production of male-only progenies when antibiotics are used in rearing diets, suggest that Wolbachia might be playing a role in sex determination in the coffee berry borer. Additional studies conþrming the elimination of the female-biased sex ratio in the progeny of coffee berry borer females treated with antibiotics, as well as detection of Wolbachia in inbreeding but not in outbreeding members of Scolytidae, would provide additional support to the hypothesis stating that Wolbachia is involved in sex determination. Acknowledgments We are grateful to the following colleagues for providing coffee berry borer specimens: Chris Lomer (Benin), Ramiro Ruõ z Cárdenas (Brazil), Maurice Tindo (Cameroon), Alex E. Bustillo Pardey (Colombia), Pablo Delgado and Alberto Larco (Ecuador), Bernard Pierre Dufour (El Salvador), Raul Muñoz and Angel Trejo (Honduras), R. Naidu, M. M. Balakrishnan, and K. Sreedharan (India), Dr. Zaenudin (Indonesia), Alford Williams (Jamaica), George I. Oduor (Kenya), Juan F. Barrera Gaytán (Mexico), Elizabeth Nuñez Sacarõ as (Peru). Nicaraguan specimens were collected by F. E. Vega, Guy Mercadier, and Edwin Zepeda; Ugandan specimens were collected by F. E. Vega, G. Mercadier, and Patrick Kucel; Dominican Republic specimens were collected by F. E. Vega. We thank Patrick F. Dowd (USDA) and two anonymous reviewers for comments on a previous version of the manuscript, and Eric Rosenquist (USDA) for making this work possible. References Cited Altschul, S. F., T. L. Madden, A. A. Schäffer, J. Zhang, Z. Zhang, W. Miller, and D. J. Lipman Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 25: 3389Ð Baker, P. S The coffee berry borer in Colombia. Final report of the DFID-Cenicafé - CABI Bioscience IPM for coffee project. DFID - Cenicafé, Chinchiná, Colombia. Barrera, J. F., J. Gómez, and C. Alauzet Can the coffee berry borer (Hypothenemus hampei) reproduce by parthenogenesis? Entomol. Exp. Appl. 77: 351Ð354. Bergamin, J Contribuição para o conhecimento da biologia da broca do café Hypothenemus hampei (Ferrari, 1867) (Col. Ipidae). Arq. Inst. Biol. 14: 31Ð72. Borsa, P., and F. Kjellberg Experimental evidence for pseudo-arrhenotoky in Hypothenemus hampei (Coleoptera: Scolytidae). Heredity 76: 130Ð135. Bourtzis, K., and S. O Neill Wolbachia infections and arthropod reproduction. BioScience 48: 287Ð293. Braig, H. R., W. Zhou, S. L. Dobson, and S. L. O Neill Cloning and characterization of a gene encoding the major surface protein of the bacterial endosymbiont Wolbachia pipientis. J. Bacteriol. 180: 2373Ð2378. Browne, F. G The biology of Malayan Scolytdae and Platypodidae. Malay. For. Rec. 22: 1Ð255. Brun, L. O., J. Stuart, V. Gaudichon, K. Aronstein, and R. H. ffrench-constant Functional haplodiploidy: a mechanism for the spread of insecticide resistance in an important international pest. Proc. Natl. Acad. Sci. U.S.A. 92: 9861Ð9865. Entwistle, P. F Inbreeding and arrhenotoky in the ambrosia beetle Xyleborus compactus (Eich.) (Coleoptera: Scolytidae). Proc. R. Entomol. Soc. Lond. A 39: 83Ð88. Feder, M. E., T. L. Karr, W. Yang, J. M. Hoekstra, and A. C. James Interaction of Drosophila and its endosymbiont Wolbachia: natural heat shock and the overcoming of sexual incompatibility. Am. Zool. 39: 363Ð373. Fialho, R. F., and L. Stevens Male-killing Wolbachia in a ßour beetle. Proc. R. Soc. Lond. Biol. Sci. 267: 1469Ð Giordano, R., J. J. Jackson, and H. M. Robertson The role of Wolbachia bacteria in reproductive incompatibilities and hybrid zones of Diabrotica beetles and Gryllus crickets. Proc. Natl. Acad. Sci. U.S.A. 94: 11439Ð Hurst, G.D.D., L. D. Hurst, and M.E.N. Majerus Cytoplasmic sex ratio distorters, pp. 125Ð154. In S. L. OÕNeill, A. A. Hoffmann, and J. H. Werren (eds.), Inßuential passengers: inherited microorganisms and arthropod reproduction. Oxford University Press, New York. Hurst, G.D.D., A. P. Johnson, J. Hinrich G. v. d. Schulenburg, and Y. Fuyama Male killing Wolbachia in Drosophila: a temperature-sensitive trait with a threshold bacterial density. Genetics 156: 699Ð709. Jeyaprakash, A., and M. Hoy Long PCR improves Wolbachia DNA ampliþcation: wsp sequences found in 76% of sixty-three arthropod species. Insect. Mol. Biol. 9: 393Ð405. Jiggins, F. M., G.D.D. Hurst, C. E. Dolman, and M.E.N. Majerus High-prevalence male-killing Wolbachia in the butterßy Acraea encedana. J. Evol. Biol. 13: 495Ð501. Kageyama, D., S. Hoshizaki, and Y. Ishikawa Femalebiased sex ratio in the Asian corn borer, Ostrinia furnacalis: evidence for the occurrence of feminizing bacteria in an insect. Heredity 81: 311Ð316. Kirkendall, L. R Ecology and evolution of biased sex ratios in bark and ambrosia beetles, pp. 235Ð345. In D. L. Wrensch and M. A. Ebbert (eds.), Evolution and diversity of sex ratio: insects and mites. Chapman & Hall, New York. Le Pelley, R. H Pests of coffee. Longmans, Green and Company, London.
5 378 ANNALS OF THE ENTOMOLOGICAL SOCIETY OF AMERICA Vol. 95, no. 3 Malloch, G., B. Fenton, and D. J. Butcher Molecular evidence for multiple infections of a new subgroup of Wolbachia in the European raspberry beetle Byturus tomentosus. Mol. Ecol. 9: 77Ð90. Muñoz, R Ciclo biológico y reproducción partenogenética de la broca del fruto del cafeto Hypothenemus hampei (Ferr.). Turrialba 39: 415Ð421. Normark, B. B., B. H. Jordal, and B. D. Farrell Origin of a haplodiploid beetle lineage. Proc. R. Soc. Lond. B 266: 2253Ð2259. Norris, D. M., and H. Chu Symbiote-dependent arrhenotokous parthenogenesis in the eurkaryote Xyleborus, pp. 453Ð460. In W. Schwemmler and H.E.A. Schenk (eds.), Endocytobiology, endosymbiosis, and cell biology, vol. 1. Walter de Gruyter, Berlin. O Neill, S. L., R. Giordano, A.M.E. Colbert, T. L. Karr, and H. M. Robertson S rrna phylogenetic analysis of the bacterial endosymbionts associated with cytoplasmic incompatibility in insects. Proc. Natl. Acad. Sci. U.S.A. 89: 2699Ð2702. O Neill, S. L., A. A. Hoffmann, and J. H. Werren (eds.) Inßuential passengers: inherited microorganisms and arthropod reproduction. Oxford University Press, New York. Peleg, B., and D. M. Norris. 1972a. Symbiotic interrelationships between microbes and ambrosia beetles. J. Invertebr. Pathol. 20: 59Ð65. Peleg, B., and D. M. Norris. 1972b. Bacterial symbiote activation of insect parthenogenetic reproduction. Nature New Biol. 236: 111Ð112. Rousset, F., D. Bouchon, B. Pintureau, P. Juchault, and M. Solignac Wolbachia endosymbionts responsible for various alterations of sexuality in arthropods. Proc. R. Soc. Lond. B 250: 91Ð98. Sasaki, T Wolbachia sp. group B (from E. kuehniella, Tsuchiura) wspb gene for surface protein, partial cds. GenBan Accession AB Stauffer, C., M.M.M. van Meer, and M. Riegler The presence of the protobacteria Wolbachia in European Ips typographus (Col., Scolytidae) populations and the consequences for genetic data. Proceedings of the German Society for General and Applied Entomology, 18Ð22 March 1997, Bayreuth, Germany. Mitt. Dtsch. Ges. Allg. Angew. Entomol. 11: 709Ð711. Stouthamer, R., R. F. Luck, and W. D. Hamilton Antibiotics cause parthenogenetic Trichogramma to revert to sex. Proc. Natl. Acad. Sci. U.S.A. 87: 2424Ð2427. Wade, M. J., and N. W. Chang Increased male fertility in Tribolium confusum beetles after infection with the intracellular parasite Wolbachia. Nature (Lond.) 373: 72Ð 74. Werren, J. H Biology of Wolbachia. Annu. Rev. Entomol. 42: 587Ð609. Werren, J. H., and D. M. Windsor Wolbachia infection frequencies in insects: evidence of a global equilibrium? Proc. R. Soc. Lond. B 267: 1277Ð1285. Werren, J. H., L. Guo, and D. Windsor Distribution of Wolbachia in neotropical arthropods. Proc. R. Soc. Lond. B 262: 197Ð204. Zchori-Fein, E., R. T. Roush, and M. S. Hunter Male production induced by antibiotic treatment in Encarsia formosa, an asexual species. Experientia 48: 102Ð105. Zhou, W., F. Rousset, and S. O Neill Phylogeny and PCR-based classiþcation of Wolbachia strains using wsp gene sequences. Proc. R. Soc. Lon. B 265: 509Ð515. Received for publication 24 August 2001; accepted 20 February 2002.
Coffee Berry Borer, Hypothenemes hampei Ferrari (Coleoptera: Scolytidae)- Microbial Interactions
Coffee Berry Borer, Hypothenemes hampei Ferrari (Coleoptera: Scolytidae)- Microbial Interactions Sayaka Aoki, Mark G Wright, Ania Wieczorek, Russell Messing, Gordon Bennett and Fernando Vega Department
More informationCatalogue of published works on. Maize Lethal Necrosis (MLN) Disease
Catalogue of published works on Maize Lethal Necrosis (MLN) Disease Mentions of Maize Lethal Necrosis (MLN) Disease - Reports and Journals Current and future potential distribution of maize chlorotic mottle
More informationFirst Report of Pierce s Disease in New Mexico
2007 Plant Management Network. Accepted for publication 20 April 2007. Published. First Report of Pierce s Disease in New Mexico Jennifer J. Randall and Maxim Radionenko, Department of Entomology, Plant
More informationLUISA MAYENS VÁSQUEZ RAMÍREZ. Adress: Cl 37 # 28-15, Manizales, Caldas, Colombia. Cell Phone Number:
LUISA MAYENS VÁSQUEZ RAMÍREZ Adress: Cl 37 # 28-15, Manizales, Caldas, Colombia. Cell Phone Number: 3013978734 E-mail: luisamayens@gmail.com PROFILE Agronomical engineer, Universidad de Caldas, Colombia.
More informationMonitoring the CBB, Using Traps, and Making Decisions about Where and What Control is Needed. Luis F. Aristizábal A. Tropical Agro-Ecology Specialist
Monitoring the CBB, Using Traps, and Making Decisions about Where and What Control is Needed Luis F. Aristizábal A. Tropical Agro-Ecology Specialist Why is it Important to Monitor the CBB? In order to
More informationUpdate on Quarantine, Containment and Biocontrol of Coffee Berry Borer
Update on Quarantine, Containment and Biocontrol of Coffee Berry Borer Neil Reimer Hawaii Department of Agriculture HDOA work on CBB Initial Response Containment/Quarantine Beauvaria registration Biological
More informationMONITORING WALNUT TWIG BEETLE ACTIVITY IN THE SOUTHERN SAN JOAQUIN VALLEY: OCTOBER 2011-OCTOBER 2012
MONITORING WALNUT TWIG BEETLE ACTIVITY IN THE SOUTHERN SAN JOAQUIN VALLEY: OCTOBER 11-OCTOBER 12 Elizabeth J. Fichtner ABSTRACT Walnut twig beetle, Pityophthorus juglandis, is the vector of thousand cankers
More information3.5 Citrus Greening (Huanglongbing) Disease in India : Present Status and Diagnostic Efforts
Page 129 3.5 Citrus Greening (Huanglongbing) Disease in India : Present Status and Diagnostic Efforts Das A. K. National Research Centre for Citrus, Amravati Road, Nagpur 440010, India. Among all diseases
More informationChapter V SUMMARY AND CONCLUSION
Chapter V SUMMARY AND CONCLUSION Coffea is economically the most important genus of the family Rubiaceae, producing the coffee of commerce. Coffee of commerce is obtained mainly from Coffea arabica and
More informationMolecular identification of bacteria on grapes and in must from Small Carpathian wine-producing region (Slovakia)
Molecular identification of bacteria on grapes and in must from Small Carpathian wine-producing region (Slovakia) T. Kuchta1, D. Pangallo2, Z. Godálová1, A. Puškárová2, M. Bučková2, K. Ženišová1, L. Kraková2
More informationREPRODUCTION, SEX RATIO AND BACTERIAL COMMUNITIES OF THE COFFEE BERRY BORER Hypothenemus hampei F. (COLEOPTERA: CURCULIONIDAE)
REPRODUCTION, SEX RATIO AND BACTERIAL COMMUNITIES OF THE COFFEE BERRY BORER Hypothenemus hampei F. (COLEOPTERA: CURCULIONIDAE) by Yobana Andrea Mariño Cárdenas A thesis submitted to the DEPARTMENT OF BIOLOGY
More informationSHORT TERM SCIENTIFIC MISSIONS (STSMs)
SHORT TERM SCIENTIFIC MISSIONS (STSMs) Reference: Short Term Scientific Mission, COST Action FA1003 Beneficiary: Bocharova Valeriia, National Scientific Center Institute of viticulture and winemaking named
More information30 YEARS OF FUEL ETHANOL PRODUCTION IN BRAZIL: identification and selection of dominant industrial yeast strains.
30 YEARS OF FUEL ETHANOL PRODUCTION IN BRAZIL: identification and selection of dominant industrial yeast strains Mário Lúcio Lopes Sugarcane Production Source: http://english.unica.com.br/content/show.asp?cntcode={d6c39d36-69ba-458d-a95c-815c87e4404d}
More informationWP Board No. 934/03. 7 May 2003 Original: English. Executive Board May 2003 London, England
WP Board No. 934/03 International Coffee Organization Organización Internacional del Café Organização Internacional do Café Organisation Internationale du Café 7 May 2003 Original: English Executive Board
More informationThe Coffee Berry Borer: Biology and Ecology
The Coffee Berry Borer: Biology and Ecology Luis F. Aristizábal Specialist on Tropical Agro- Ecology Mid-Florida Research and Education Center University of Florida Kona- Hawaii USA August 24-25, 2012
More informationICC September 2009 Original: English. International Coffee Council 103 rd Session September 2009 London, England
ICC 103-4 11 September 2009 Original: English E International Coffee Council 103 rd Session 23 25 September 2009 London, England Impact evaluation of the Integrated Management of the Coffee Berry Borer
More informationECOLOGY AND BIOLOGY OF IN BRAZIL
ECOLOGY AND BIOLOGY OF IN BRAZIL José Roberto P. Parra Mariuxi Lorena Gomez Torres Dori Edson Nava Paulo Eduardo Branco Paiva USDA, APHIS International Workshop on Tamarixia species McAllen, TX, Feb. 2-4,
More informationEXPLORING FOR PERSEA IN LATIN AMERICA
California Avocado Society 1978 Yearbook 62: 60-65 EXPLORING FOR PERSEA IN LATIN AMERICA Eugenio Schieber and G. A. Zentmyer Plant Pathologist, Antigua, Guatemala; and Professor, Department of Plant Pathology,
More informationPRUNUS AMERICANA (ROSACEAE) IN THE ARKANSAS FLORA
Johnson, G.P. 2013. Prunus americana (Rosaceae) in the Arkansas flora. Phytoneuron 2013-33: 1 5. Published 20 May 2013. ISSN 2153 733X PRUNUS AMERICANA (ROSACEAE) IN THE ARKANSAS FLORA GEORGE P. JOHNSON
More informationTitle: Genetic Variation of Crabapples ( Malus spp.) found on Governors Island and NYC Area
Title: Genetic Variation of Crabapples ( Malus spp.) found on Governors Island and NYC Area Team Members: Jianri Chen, Zinan Ma, Iulius Sergiu Moldovan and Xuanzhi Zhao Sponsoring Teacher: Alfred Lwin
More informationCoffee: World Markets and Trade
United States Department of Agriculture Foreign Agricultural Service Circular Series December 213 Coffee: World Markets and Trade 4 in Producing Countries to Continue Rising Million 6 Kilogram Bags 3 2
More informationLETTER FROM THE EXECUTIVE DIRECTOR
E LETTER FROM THE EXECUTIVE DIRECTOR COFFEE MARKET REPORT December 2008 Price levels in December confirmed the downward trend recorded in the coffee market since September 2008. The monthly average of
More informationUTZ Coffee Statistics Report 2017
UTZ Coffee Statistics Report 2017 UTZ is the largest certification program in the world for sustainable coffee More than 480,000 coffee farmers and workers benefit from the UTZ program UTZ certified coffee
More informationAPPENDIX Thirty Trees Sampling Method for CBB Monitoring
APPENDIX Thirty Trees Sampling Method for CBB Monitoring Based on a Presentation by Luis F. Aristizábal (University of Florida) Revised: January 2014 by CBB Summit Committee I t is not enough to know that
More informationTitle: Development of Simple Sequence Repeat DNA markers for Muscadine Grape Cultivar Identification.
Title: Development of Simple Sequence Repeat DNA markers for Muscadine Grape Cultivar Identification. Progress Report Grant Code: SRSFC Project # 2018 R-06 Research Proposal Name, Mailing and Email Address
More informationUsing Beauveria as part of an integrated approach for control of coffee berry borer
Using Beauveria as part of an integrated approach for control of coffee berry borer Robert Hollingsworth Research Entomologist US Pacific Basin Agricultural Research Center Outline of Talk Relative importance
More informationA Preliminary Report on a Method of Biological Control of the Chestnut Blight Not Involving the Use of a Hypovirulent Strain of Endothia parasitica
A Preliminary Report on a Method of Biological Control of the Chestnut Blight Not Involving the Use of a Hypovirulent Strain of Endothia parasitica W. H. Weidlich Department of Botany & Plant Pathology,
More informationTHOUSAND CANKERS DISEASE AND WALNUT TWIG BEETLE IN A THREE YEAR OLD ORCHARD, SOLANO COUNTY
THOUSAND CANKERS DISEASE AND WALNUT TWIG BEETLE IN A THREE YEAR OLD ORCHARD, SOLANO COUNTY Carolyn DeBuse, Andrew Johnson, Stacy Hishinuma, Steve Seybold, Rick Bostock, and Tatiana Roubtsova ABSTRACT Some
More informationINTRODUCTION OF ALLORHOGAS PYRALOPHAGUS MARSH (BRACONIDAE) IN LAMPUNG (INDONESIA) WITH PRELIMINARY NOTES Oh, ITS BIOLOGY
Entomology I INTRODUCTION OF ALLORHOGAS PYRALOPHAGUS MARSH (BRACONIDAE) IN LAMPUNG (INDONESIA) WITH PRELIMINARY NOTES Oh, ITS BIOLOGY Ubandi Harsanto and Sunaryo Research Division, P.T. Gunung Madu Plantations,
More informationGet serious about your approach to Botrytis management
Australia Get serious about your approach to Botrytis management 21.11.2017 Botrytis is an opportunistic pathogen which can develop on damaged tissue, such as that caused by Light Brown Apple Moth or LBAM
More informationOutlook for the World Coffee Market
Outlook for the World Coffee Market 8 th AFRICAN FINE COFFEE CONFERENCE & EXHIBITION 17 to 19 February 2011 Arusha, Tanzania José Sette Executive Director a.i. 225 ICO composite indicator price Monthly:
More informationVineyard IPM Scouting Report for week of 14 May 2012 UW-Extension Door County and Peninsular Agricultural Research Station Sturgeon Bay, WI
NO. 5 1 Vineyard IPM Scouting Report for week of 14 May 2012 UW-Extension Door County and Peninsular Agricultural Research Station Sturgeon Bay, WI Grape Phylloxera Although phylloxera leaf galls have
More informationCropCast Global Coffee Estimates
CropCast Weekly Report Kenny Miller Tuesday, June 06, 2017 CropCast Global Estimates AREA (th hectares) YIELD (kg/ha) PRODUCTION (M bags) 16/17 17/18 16/17 17/18 16/17 17/18 South America 4281 4206 994
More informationVineyard Insect Management what does a new vineyard owner/manager need to know?
Vineyard Insect Management what does a new vineyard owner/manager need to know? Keith Mason and Rufus Isaacs Department of Entomology, Michigan State University masonk@msu.edu isaacsr@msu.edu Insect management
More informationImport Volume of coffee into Japan (metric tons) Total Green Beans equivalent. Soluble Coffee
Market in Japan -111- History 1. A limited amount of coffee was drunk by Dutch residents of Nagasaki at the end of the eighteenth century. However, coffee was not generally sold until the last quarter
More informationRevised World Coffee Production Forecast Remains on Track for Record 140
United States Department of Agriculture Foreign Agricultural Service Circular Series FTROP 4-8 December 28 Revised World Coffee Forecast Remains on Track for Record 14 12 Million 6 kilo bags 1 8 6 4 2
More informationCoffee: World Markets and Trade
United States Department of Agriculture Foreign Agricultural Service June 2017 Coffee: World Markets and Trade 2017/18 Forecast Overview World coffee production for 2017/18 is forecast at 159 million bags
More informationUTZ Cocoa Statistics Report 2017
UTZ Cocoa Statistics Report 2017 UTZ is the largest program in the world for sustainable cocoa There are more than 760,000 cocoa farmers in the UTZ program UTZ certified cocoa is produced in 21 countries
More informationA brief history of Cactoblastis cactorum and its effects on Florida native Opuntia
A brief history of Cactoblastis cactorum and its effects on Florida native Opuntia Heather Jezorek Peter Stiling University of South Florida, Tampa, Florida, USA Cactoblastis cactorum - Intro Family Pyralidae
More informationPreliminary observation on a spontaneous tricotyledonous mutant in sunflower
Preliminary observation on a spontaneous tricotyledonous mutant in sunflower Jinguo Hu 1, Jerry F. Miller 1, Junfang Chen 2, Brady A. Vick 1 1 USDA, Agricultural Research Service, Northern Crop Science
More informationMANAGING the COFFEE BERRY BORER in the Home Garden
MANAGING the COFFEE BERRY BORER in the Home Garden West Hawaii Master Gardeners 2013 Updated 3.31.14 coffee berry borer female (1.4-1.78 mm) CBB - A bark beetle endemic to Central Africa Their life cycle
More informationBEEF Effect of processing conditions on nutrient disappearance of cold-pressed and hexane-extracted camelina and carinata meals in vitro 1
BEEF 2015-05 Effect of processing conditions on nutrient disappearance of cold-pressed and hexane-extracted camelina and carinata meals in vitro 1 A. Sackey 2, E. E. Grings 2, D. W. Brake 2 and K. Muthukumarappan
More informationUse of RAPD and SCAR markers for identification of strawberry genotypes carrying red stele (Phytophtora fragariae) resistance gene Rpf1
Agronomy Research 4(Special issue), 335 339, 2006 Use of RAPD and SCAR markers for identification of strawberry genotypes carrying red stele (Phytophtora fragariae) resistance gene Rpf1 R. Rugienius*,
More informationDetection, Rapid Response and Containment of Coffee Berry Borer
Detection, Rapid Response and Containment of Coffee Berry Borer Neil Reimer Hawaii Department of Agriculture Coffee History in Hawaii 1825 first established trees, Oahu 1836 first plantation, Kauai Late
More informationCurrent research status and strategic challenges on the black coffee twig borer, Xylosandrus compactus in Uganda
Current research status and strategic challenges on the black coffee twig borer, Xylosandrus compactus in Uganda Dr. Godfrey Kagezi (PhD) Senior Research Officer/Plant Entomologst National Coffee Research
More informationCoffee: World Markets and Trade
United States Department of Agriculture Foreign Agricultural Service June 2016 Coffee: World Markets and Trade 2016/17 Forecast Overview World coffee production for 2016/17 is forecast to rise 2.4 million
More informationInterloper s legacy: invasive, hybrid-derived California wild radish (Raphanus sativus) evolves to outperform its immigrant parents
Interloper s legacy: invasive, hybrid-derived California wild radish (Raphanus sativus) evolves to outperform its immigrant parents Caroline E. Ridley 1 and Norman C. Ellstrand 1,2 1 Department of Botany
More informationMONTHLY COFFEE MARKET REPORT
E MONTHLY COFFEE MARKET REPORT December 2011 In December 2011 Arabica prices underwent significant downward corrections while Robusta prices increased slightly. The average of the 2 nd and 3 rd positions
More informationMANAGING the COFFEE BERRY BORER in the Home Garden. West Hawaii Master Gardeners 2013
MANAGING the COFFEE BERRY BORER in the Home Garden West Hawaii Master Gardeners 2013 coffee berry borer female (1.4-1.78 mm) CBB - A bark beetle endemic to Central Africa Their life cycle inside the host
More informationPapaya. Carica. Papaya Readings (On web page) Paw paw Papaw Family Caricaceae Genus Carica Species papaya
Papaya Paw paw Papaw Family Caricaceae Genus Carica Species papaya Papaya Readings (On web page) Nishina et al., 2. Papaya Production in Hawaii. CTAHR, F&N-3. Manshardt, 1999. UH Rainbow Papaya. A High-Quality
More information> $500 MILLION! The Tropics. The coffee berry borer: a short review with recent findings and future research directions
The coffee berry borer: a short review with recent findings and future research directions Coffee berry borer Hypothenemus hampei Fernando E. Vega Sustainable Perennial Crops Laboratory, USDA, ARS Beltsville,
More informationALBINISM AND ABNORMAL DEVELOPMENT OF AVOCADO SEEDLINGS 1
California Avocado Society 1956 Yearbook 40: 156-164 ALBINISM AND ABNORMAL DEVELOPMENT OF AVOCADO SEEDLINGS 1 J. M. Wallace and R. J. Drake J. M. Wallace Is Pathologist and R. J. Drake is Principle Laboratory
More information2005 Research: Monitoring, Sanitation, and Insect Pest Management in Figs
Fig Day 2006 2005 Research: Monitoring, Sanitation, and Insect Pest Management in Figs Chuck Burks 1, David Brandl 1, Themis Michailides 2, and Mark Doster 2 1 USDA-ARS, Parlier, CA 2 UC Kearney Agricultural
More informationGENETICS AND EVOLUTION OF CORN. This activity previews basic concepts of inheritance and how species change over time.
GENETICS AND EVOLUTION OF CORN This activity previews basic concepts of inheritance and how species change over time. Objectives for Exam #1: 1. Describe and complete a monohybrid ( one trait ) cross of
More informationSpotted wing drosophila in southeastern berry crops
Spotted wing drosophila in southeastern berry crops Hannah Joy Burrack Department of Entomology entomology.ces.ncsu.edu facebook.com/ncsmallfruitipm @NCSmallFruitIPM Spotted wing drosophila Topics Biology
More informationA Brief Introduction to the Cactus Moth (Cactoblastis cactorum) and its Threat to the local Prickly Pear (Opuntia) Cactus Species
A Brief Introduction to the Cactus Moth (Cactoblastis cactorum) and its Threat to the local Prickly Pear (Opuntia) Cactus Species By Philip Rose Natural History Division Institute of Jamaica Plants belonging
More informationLETTER FROM THE EXECUTIVE DIRECTOR COFFEE MARKET REPORT. November 2004
International Coffee Organization Organización Internacional del Café Organização Internacional do Café Organisation Internationale du Café E LETTER FROM THE EXECUTIVE DIRECTOR COFFEE MARKET REPORT November
More informationMONTHLY COFFEE MARKET REPORT
E MONTHLY COFFEE MARKET REPORT July 2012 Coffee prices rebounded in July 2012, with the ICO composite indicator increasing by 9.5% compared to June, following nine consecutive months of price falls. The
More informationIdentification and Classification of Pink Menoreh Durian (Durio Zibetinus Murr.) Based on Morphology and Molecular Markers
RESEARCH Identification and Classification of Pink Durian (Durio Zibetinus Murr.) Based on Morphology and Molecular Markers Nandariyah a,b * adepartment of Agronomy, Faculty of Agriculture, Sebelas Maret
More informationInvasive Ambrosia Beetle Conference The Situation in California August 12 14, 2012
Invasive Ambrosia Beetle Conference The Situation in California August 12 14, 2012 Meeting sponsored by: The Hofshi Foundation University of California, Riverside UC Center for Invasive Pest Research The
More informationICO Coffee Berry Borer Seminar. Coffee berry borer triple-action integrated pest management
ICO Coffee Berry Borer Seminar London, 17 March 2009 Coffee berry borer triple-action integrated pest management Bernard Pierre Dufour CIRAD France Key words Coffee berry borer, IPM, agronomic control,
More informationA Computational analysis on Lectin and Histone H1 protein of different pulse species as well as comparative study with rice for balanced diet
www.bioinformation.net Hypothesis Volume 8(4) A Computational analysis on Lectin and Histone H1 protein of different pulse species as well as comparative study with rice for balanced diet Md Anayet Hasan,
More informationMONTHLY COFFEE MARKET REPORT
E MONTHLY COFFEE MARKET REPORT February 2014 February 2014 has seen significant developments in the coffee market, with prices shooting upwards at a startling rate. The ICO composite daily price has increased
More informationSweet corn insect management by insecticides in Ohio, 2015 Final report 12/31/2015
Sweet corn insect management by insecticides in Ohio, 2015 Final report 12/31/2015 Celeste Welty, Associate Professor, Department of Entomology, The Ohio State University, Rothenbuhler Laboratory, 2501
More informationFY2012 Final report to the Virginia Wine Board
FY2012 Final report to the Virginia Wine Board Documentation of Grapevine leafroll-associated viruses and other major grape viruses in wine grape varieties and native grape species in Virginia, and examination
More informationPetite Mutations and their Impact of Beer Flavours. Maria Josey and Alex Speers ICBD, Heriot Watt University IBD Asia Pacific Meeting March 2016
Petite Mutations and their Impact of Beer Flavours Maria Josey and Alex Speers ICBD, Heriot Watt University IBD Asia Pacific Meeting March 2016 Table of Contents What Are They? No or reduced mitochondrial
More informationMONTHLY COFFEE MARKET REPORT
E MONTHLY COFFEE MARKET REPORT July 2011 Downward corrections to coffee prices recorded in June continued during July 2011, as the monthly average of the ICO composite indicator price fell to 210.36 US
More informationFood Allergen and Adulteration Test Kits
Food Allergen and Adulteration Test Kits Overview Neogen offers food allergen test kits to detect almond, egg, gliadin, hazelnut, milk, mustard, peanut, sesame, shellfish, soy and walnut residues (see
More informationJUNPERUS VIRGINIANA IN THE SERRANIAS DEL BURRO MOUNTAINS, COAHUILA, MEXICO: A PLEISTOCENE RELICT
168 Phytologia (August 2011) 93(2) JUNPERUS VIRGINIANA IN THE SERRANIAS DEL BURRO MOUNTAINS, COAHUILA, MEXICO: A PLEISTOCENE RELICT Robert P. Adams Biology Department, Baylor University, Box 97388, Waco,
More informationWhere in the Genome is the Flax b1 Locus?
Where in the Genome is the Flax b1 Locus? Kayla Lindenback 1 and Helen Booker 2 1,2 Plant Sciences Department, University of Saskatchewan, Saskatoon, SK S7N 5A8 2 Crop Development Center, University of
More informationShazia Mannan COMSATS Institute of Information Technology Sahiwal Campus, Pakistan
Shazia Mannan COMSATS Institute of Information Technology Sahiwal Campus, Pakistan Citrus is one of the major export commodities of Pakistan and is grown in an area of 160,000 ha. Annual production of
More informationVineyard IPM Scouting Report for week of 26 July 2010 UW-Extension Door County and Peninsular Agricultural Research Station Sturgeon Bay, WI
Vineyard IPM Scouting Report for week of 26 July 2010 UW-Extension Door County and Peninsular Agricultural Research Station Sturgeon Bay, WI Multi-colored Asian lady beetle (MALB) Harmonia axyridis Of
More informationMUMmer 2.0. Original implementation required large amounts of memory
Rationale: MUMmer 2.0 Original implementation required large amounts of memory Advantages: Chromosome scale inversions in bacteria Large scale duplications in Arabidopsis Ancient human duplications when
More informationLETTER FROM THE EXECUTIVE DIRECTOR
E LETTER FROM THE EXECUTIVE DIRECTOR COFFEE MARKET REPORT August 2009 Although the monthly average of the ICO composite indicator price increased by 4% in August, from 112.90 US cents per lb in July to
More informationStatus of Halyomorpha halys in the Mid-Atlantic U.S. Tom Kuhar Professor and Vegetable Entomology Specialist Department of Entomology, Virginia Tech
Status of Halyomorpha halys in the Mid-Atlantic U.S. Tom Kuhar Professor and Vegetable Entomology Specialist Department of Entomology, Virginia Tech Mid-Atlantic Researchers Providing Input Tom Kuhar &
More informationLETTER FROM THE EXECUTIVE DIRECTOR
E LETTER FROM THE EXECUTIVE DIRECTOR COFFEE MARKET REPORT January 2010 The upward trend in coffee prices recorded in December2009 continued during the first fortnight of 2010, with the monthly average
More informationField Testing Transgenic Grapevine for Bacterial and Fungal Disease Resistance
Field Testing Transgenic Grapevine for Bacterial and Fungal Disease Resistance D J Gray, Z T Li, S A Dhekney, M Dutt, D L Hopkins Mid-Florida Research & Education Center University of Florida/IFAS T W
More informationEffectiveness of the CleanLight UVC irradiation method against pectolytic Erwinia spp.
Page 1 of 12 Effectiveness of the CleanLight UVC irradiation method against pectolytic Erwinia spp. Zon Fruit & Vegetables Author: Agnieszka Kaluza Innovation & Development Engineer 29 November 2013 Versie:
More informationEmerging Foodborne Pathogens with Potential Significance to the Middle East
Emerging Foodborne Pathogens with Potential Significance to the Middle East Ahmed E. Yousef Department of Food Science and Technology (and Department of Microbiology) The Ohio State University Columbus,
More informationLUIS FERNANDO ARISTIZÁBAL
LUIS FERNANDO ARISTIZÁBAL 3234 Fawnwood Dr Ocoee, Florida 34761 Phone number: 813 863 4995 E-mail: larist@ufl.edu Education 2005. Santa Rosa de Cabal University. 38 Semester Credit Hours of graduate study
More information12. A Cytogenetic Assessment on the Origin o f the Gold. fish
58 Proc. Japan Acad., 55, Ser. B (1979) [Vol. 55(B), 12. A Cytogenetic Assessment on the Origin o f the Gold. fish By Yoshio OJIMA, Takayoshi USDA, and Toshitaka NARIKAWA Department of Biology, Faculty
More informationDNA extraction method as per QIAamp DNA mini kit (Qiagen, Germany)
APPENDIX 3 (MOLECULAR TECHNIQUES) 3.2.2a) DNA extraction method as per QIAamp DNA mini kit (Qiagen, Germany) Two hundred microliters (200 µl) of the EDTA blood was added to 200 µl of Buffer AL and 20 µl
More informationSTEM-END ROTS : INFECTION OF RIPENING FRUIT
1 STEM-END ROTS : INFECTION OF RIPENING FRUIT K.R. EVERETT The Horticulture and Food Research Institute of New Zealand Ltd. Private Bag 919, Mt Albert, Auckland ABSTRACT Fruit from an unsprayed orchard
More informationDifferences in virulence of Phytophthora capsici isolates from a worldwide collection on tomato fruits
Euro. J. Plant Pathol. DOI:10.1007/s10658-011-9873-4 Online First Differences in virulence of Phytophthora capsici isolates from a worldwide collection on tomato fruits Dr. Leah Granke Dr. Lina Quesada-Ocampo
More informationIMPORTATION OF NELUMBO NUCIFERA
IMPORTATION OF NELUMBO NUCIFERA GAERTNER (WATERLILY, LOTUS) AS ROOTS FROM EL SALVADOR, GUATEMALA, HONDURAS AND NICARAGUA INTO THE CONTINENTAL UNITED STATES A Qualitative, Pathway-Initiated Risk Assessment
More informationProceedings of The World Avocado Congress III, 1995 pp
Proceedings of The World Avocado Congress III, 1995 pp. 335-339 SENSITIVITY OF AVOCADO FRUIT TO ETHYLENE P.J. Hofman, R.L. McLauchlan and L.G. Smith Horticulture Postharvest Group Department of Primary
More informationMuseum Victoria CRC National Plant Biosecurity
1. PaDIL Species Factsheet Scientific Name: Ralstonia solanacearum (Smith 1896) Yabuuchi et al. 1996 race 2 (Bacteria: Proteobacteria: Burkholderiales: Burkholderiaceae) Common Name Moko disease of banana
More informationDiaspidid scale insects on imported fruit are a substantial risk
Diaspidid scale insects on imported fruit are a substantial risk Richard Stouthamer and Joseph Morse Department of Entomology, University of California, Riverside USDA finding: Living scales present on
More informationAllergy Awareness and Management Policy
Allergy Awareness and Management Policy Overview This policy is concerned with a whole school approach to the health care management of those members of our school community suffering from specific allergies.
More informationReevaluation of Phomopsis species affecting sunflowers in the United States
Reevaluation of Phomopsis species affecting sunflowers in the United States Febina Mathew, Erik Heitkamp, Sam Markell, Kholoud Alananbeh, Nikolay Balbyshev, Lisa Castlebury, and Thomas Gulya Phomopsis
More informationComparison of the Improved Coconut Hybrid CRIC65 with its Reciprocal Cross and the Parental Varieties for Reproductive Traits
Journal of Food and Agriculture 2014, 7 (1 & 2): 11-17 DOI: http://doi.org/10.4038/jfa.v7i1-2.5189 Comparison of the Improved Coconut Hybrid CRIC65 with its Reciprocal Cross and the Parental Varieties
More informationWorm Collection. Prior to next step, determine volume of worm pellet.
Reinke Lab ChIP Protocol (last updated by MK 05/24/13) Worm Collection 1. Collect worms in a 50ml tube. Spin and wait until worms are collected at the bottom. Transfer sample to a 15ml tube and wash with
More informationICC July 2010 Original: French. Study. International Coffee Council 105 th Session September 2010 London, England
ICC 15-2 12 July 21 Original: French Study E International Coffee Council 15 th Session 22 24 September 21 London, England Relations between coffee stocks and prices Background In the context of its programme
More information2009 SUNFLOWER INSECT PEST PROBLEMS AND INSECTICIDE UPDATE
2009 SUNFLOWER INSECT PEST PROBLEMS AND INSECTICIDE UPDATE Janet Knodel 1, Larry Charlet 2, Patrick Beauzay 1 and Theresa Gross 2 1 NDSU, School of Natural Resource Sciences Entomology, Fargo, ND 2 USDA-ARS,
More informationVolume XIV, Number 2 January 13, What is the source of potato psyllids colonizing Washington, Oregon, and Idaho potato fields?
Research & Extension for the Potato Industry of Idaho, Oregon, & Washington Andrew Jensen, Editor. ajensen@potatoes.com; 208-939-9965 www.nwpotatoresearch.com Volume XIV, Number 2 January 13, 2014 What
More informationVirus complexes in strawberry: What are they and how do we manage them?
Virus complexes in strawberry: What are they and how do we manage them? William M. Wintermantel USDA-ARS Salinas, CA 93905 Ph: 831-755-2824 bill.wintermantel@ars.usda.gov Robert R. Martin USDA-ARS HCRL
More informationDraft guide to Identification of Coffee berry borer from similar bark beetles in Papua New Guinea (Version 0.1)
Draft guide to Identification of Coffee berry borer from similar bark beetles in Papua New Guinea (Version 0.1) Andrew Johnson, Craig Bateman and Jiri Hulcr Introduction The coffee berry borer (CBB), Hypothenemus
More informationICC September 2018 Original: English. Emerging coffee markets: South and East Asia
ICC 122-6 7 September 2018 Original: English E International Coffee Council 122 st Session 17 21 September 2018 London, UK Emerging coffee markets: South and East Asia Background 1. In accordance with
More informationcone and seed insects -specialists in highly nutritious structures -life cycle closely tied to reproductive structure development
cone and seed insects -specialists in highly nutritious structures -life cycle closely tied to reproductive structure development may impact natural regeneration importance of seed and cone insects seed
More informationDNA-Miniprep. - Rapid boiling
DNA-Miniprep. - Rapid boiling by A. Untergasser (contact address and download at www.untergasser.de/lab) Version: 1.0 - Print Version (.PDF) ATTENTION: This is a low priced protocol. Use it preferably!
More information