Response of ALS-resistant Alopecurus myosuroides to different herbicide strategies in a crop rotation including imidazolinone-tolerant oilseed rape

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1 Response of ALS-resistant Alopecurus myosuroides to different herbicide strategies in a crop rotation including imidazolinone-tolerant oilseed rape Anja Löbmann, Jan Petersen

2 Aim of the study Is it possible to integrate CLEARFIELD winter oil-seed rape in a crop rotation without increasing the risk of development of ALS resistant weeds by adapting the herbicide strategy? EWRS Working Group Herbicide Tolerant Varieties 2

3 Materials and Methods field trials: crop rotation with four replications at 2 locations winter oil-seed rape (Ra) winterwheat (WW) winterwheat (SW) seeding defined amounts of: ALOMY: sensitive and resistant Alopecurus myosuroides (ALS 574) at begin of the trial (2014) Herbicide strategies: 1. Use of ALS-inhibitors in all cultures and years 2. Use of ALS-inhibitors only in oil-seed rape 3. Use of ALS-inhibitors in 2 of 3 years 4. No use of ALS-inhibitors EWRS Working Group Herbicide Tolerant Varieties 3

4 Materials and Methods Use of ALS-inhibitors Herbicide Strategies Oil-seed rape Winter wheat (WW) Winter wheat (SW) EWRS Working Group Herbicide Tolerant Varieties 4

5 Trial site Bingen in May 2016 trial design EWRS Working Group Herbicide Tolerant Varieties 5

6 Materials and Methods counting of weeds plants / m² and ALOMY ears/m² genetic analysis of surviving plants examination for target-site resistance Trp574 (ALOMY) EWRS Working Group Herbicide Tolerant Varieties 6

7 ALOMY ears / m Ra / WW / SW 1 Ra / WW / SW 2 Ra / WW / SW 3 Ra / WW / SW 4 WW / SW / Ra 1 WW / SW / Ra 2 WW / SW / Ra 3 WW / SW / Ra 4 SW / Ra / WW 1 SW / Ra / WW 2 SW / Ra / WW 3 SW / Ra / WW 4 Ra 2015 WW 2016 SW 2017 WW 2015 SW 2016 Ra 2017 SW 2015 Ra 2016 WW 2017 Results - ALOMY Head density site: Bingen EWRS Working Group Herbicide Tolerant Varieties 7

8 Results - ALOMY Head density 800 ALOMY ears / m Ra / WW / SW 1 Ra / WW / SW 2 Ra / WW / SW 3 Ra / WW / SW 4 WW / SW / Ra 1 WW / SW / Ra 2 WW / SW / Ra 3 WW / SW / Ra 4 SW / Ra / WW 1 SW / Ra / WW 2 SW / Ra / WW 3 SW / Ra / WW 4 Ra 2015 WW 2016 SW 2017 WW 2015 SW 2016 Ra 2017 SW 2015 Ra 2016 WW 2017 site: Thiemendorf Ears / m 2 EWRS Working Group Herbicide Tolerant Varieties 8 in Thiemendorf

9 Winter oil-seed rape on 12 th October, 2016 no ALS-inhibitors EWRS Working Group Herbicide Tolerant Varieties 9

10 site Thiemendorf May EWRS Working Group Herbicide Tolerant Varieties 10

11 Number of ALOMY plants / m T 1: WW 2015 T 1: SW 2016 T 1: Ra 2017 T 2: WW 2015 N T 2: SW T 2: Ra 2017 T 1: WW 2015 T 3: WW 2015 T 1: SW 2016 T 3: SW 2016 T 1: Ra 2017 T 3: Ra 2017 T 2: WW 2015 T 4: WW 2015 T 4: SW 2016 T 2: SW 2016 T 4: Ra 2017 T 2: Ra 2017 T 3: WW 2015 T 3: SW 2016 Results Analysis of TSR T 3: Ra 2017 site: Bingen T 4: WW 2015 T 4: SW 2016 T 4: Ra 2017 number of surviving plants ALOMY plants with TSR number of surviving plants ALOMY plants with TSR number of surviving plants ALOMY plants with TSR number of surviving plants ALOMY plants with TSR EWRS Working Group Herbicide Tolerant Varieties 11

12 Number of ALOMY plants / m T 1: WW 2015 T 1: SW 2016 T 1: Ra 2017 T 2: WW 2015 T 2: SW 2016 T 2: Ra 2017 T 3: WW 2015 T 3: SW 2016 number of surving plants ALOMY plants with TSR number of surviving plants ALOMY plants with TSR number of surviving plants ALOMY plants with TSR number of surviving plants ALOMY plants with TSR T 3: Ra 2017 T 4: WW 2015 T 4: SW 2016 T 4: Ra 2017 Results Analysis of TSR site: Thiemendorf EWRS Working Group Herbicide Tolerant Varieties 12

13 Conclusion ALOMY population density depends on site spefic conditions (climate, crop-weed interaction, ) after three years ALOMY is not under control anymore in most treatments in treatment 1 only ALS-inhibitors situation is worst however, CL-OSR does not increase resistance situation driving factor is rotation of MoA in the crop rotation/herbicide strategy (e.g. use of propazamide in oil seed rape) EWRS Working Group Herbicide Tolerant Varieties 13

14 Thank you for your attention supported by BASF SE EWRS Working Group Herbicide Tolerant Varieties 14

15 Oilseed Rape Herbicide Strategies Materials and Methods pre-emergence BBCH 12 BBCH 14 1 Butisan Kombi (2,5 l/ha) 200 g/l metazachlor [K3] 200 g/l dimethenamid-p [K3] 2 Vantiga D (2,0 l/ha) 375 g/l metazachlor [K3] + Dash E.C. (1,0 l/ha) auxilary material Clentiga (1,0 l/ha) 12,5 g/l imazamox [B] g/l quinmerac [O] + Dash E.C. (1,0 l/ha) auxilary material Focus Ultra (1,5 l/ha) 100 g/l cycloxydim [A] + Dash E.C. (1,0 l/ha) auxilary material 3 Clentiga (1,0 l/ha) 12,5 g/l imazamox [B] g/l quinmerac [O] + Runway (0,2 l/ha) 240 g/l clopyralid [O] + 80 g/l picloram [O] + 40 g/l aminopyralid [O] + Dash E.C. (1,0 l/ha) auxilary material + Kerb Flo (1,875 l/ha) 400 g/l propyzamid [K1] 4 Butisan Gold (2,5 l/ha) 200 g/l metazachlor [K3] 200 g/l dimethylenamid-p [K3]] 100 g/l quinmerac [O] EWRS Working Group Herbicide Tolerant Varieties Focus Ultra (1,5 l/ha) 100 g/l cycloxydim [A] + Dash E.C. (1,0 l/ha) auxilary material + Kerb Flo (1,875 l/ha) 400 g/l propyzamid [K1] 15

16 Herbicide Strategies WW pre-emergence postemergence 1 postemergence 2 1 Atlantis WG (400 g/ha) 30 g/kg mesosulfuron-methyl [B] + 6 g/kg iodosulfuron-methyl-sodium [B] + 90 g/kg mefenpyr-diethyl (safener) + Biathlon 4 D (70 g/ha) 714 g/kg tritosulfuron [B] + 54 g/kg florasulfuron [B] + Dash E.C. (1,0 l/ha) auxilary material 2 Malibu (4,0 l/ha) 300 g/l pendimethalin [K1] 60 g/l flufenacet [K3] Axial 50 (1,2 l/ha) 50 g/l pinoxaden [A] 12,5 g/l cloquintocet-methyl [A] 3 Atlantis WG (400 g/ha) 30 g/kg mesosulfuron-methyl [B] + 6 g/kg iodosulfuron-methyl-sodium [B] + 90 g/kg mefenpyr-diethyl (safener) + Biathlon 4 D (70 g/ha) 714 g/kg tritosulfuron [B] + 54 g/kg florasulfuron [B] + Dash E.C. (1,0 l/ha) auxilary material 4 Malibu (4,0 l/ha) 300 g/l pendimethalin [K1] 60 g/l flufenacet [K3] Axial 50 (1,2 l/ha) 50 g/l pinoxaden [A] 12,5 g/l cloquintocet-methyl [A] EWRS Working Group Herbicide Tolerant Varieties Duanti (1,0 l/ha) 40 g/l fluroxypyr [O] 200 g/l MCPA [O] 20 g/l clopyralid [O] Duanti (1,0 l/ha) 40 g/l fluroxypyr [O] 200 g/l MCPA [O] 20 g/l clopyralid [O] 16

17 Herbicide Strategies SW pre-emergence postemergence 1 postemergence 2 1 Atlantis WG (400 g/ha) 30 g/kg mesosulfuron-methyl [B] + 6 g/kg iodosulfuron-methyl-sodium [B] + 90 g/kg mefenpyr-diethyl (safener) + Biathlon 4 D (70 g/ha) 714 g/kg tritosulfuron [B] + 54 g/kg florasulfuron [B] + Dash E.C. (1,0 l/ha) auxilary material 2 Malibu (4,0 l/ha) 300 g/l pendimethalin [K1] 60 g/l flufenacet [K3] 3 Malibu (4,0 l/ha) 300 g/l pendimethalin [K1] 60 g/l flufenacet [K3] Axial 50 (1,2 l/ha) 50 g/l pinoxaden [A] 12,5 g/l cloquintocet-methyl [A] Axial 50 (1,2 l/ha) 50 g/l pinoxaden [A] 12,5 g/l cloquintocet-methyl [A] Duanti (1,0 l/ha) 40 g/l fluroxypyr [O] 200 g/l MCPA [O] 20 g/l clopyralid [O] Duanti (1,0 l/ha) 40 g/l fluroxypyr [O] 200 g/l MCPA [O] 20 g/l clopyralid [O] 4 Malibu (4,0 l/ha) 300 g/l pendimethalin [K1] 60 g/l flufenacet [K3] Axial 50 (1,2 l/ha) 50 g/l pinoxaden [A] 12,5 g/l cloquintocet-methyl [A] Duanti (1,0 l/ha) 40 g/l fluroxypyr [O] 200 g/l MCPA [O] 20 g/l clopyralid [O] EWRS Working Group Herbicide Tolerant Varieties 17