Information on Xylella fastidiosa in Germany (update) Xylella fastidiosa in Germany, information PAFF,

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Sydney Watson
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1 Information on Xylella fastidiosa in Germany (update) Xylella fastidiosa in Germany, information PAFF,

2 Surveillance Survey on specified plants in 100m radius Survey and record keeping on specified plants within a radius of 100m around the plant which has been tested and found to be infected Sampling and testing of specified plants (update: 13 July) 87 samples from different specified plants tested (all negative) 17 samples still under investigation Still only one plant (oleander) has been found to be infested with X. fastidiosa! Xylella fastidiosa in Germany, information PAFF,

3 13 July: Sampling and testing 100m radius Green negative Violett sampled, result pending White to be sampled Xylella fastidiosa in Germany, information PAFF,

4 13 July: results for specified plants 100m radius 87 samples tested negative species Number of samples Acer 3 Aspidistra elatior 1 Brassica oleracea var. 3 gongylodes Capsella bursa-pastoris 2 Capsicum 1 Catharanthus 1 Citrus (potted plant) 1 Fragaria ananassa 1 Pelargonium 1 Gingko 1 Genista 1 Hedera helix 5 Helianthus annuum 2 Helichrysum italicum 1 Hemerocallis 3 Hibiscus syriacus 1 Lavandula 1 Lobularia 5 Lonicera 1 Melissa officinalis 1 Species Number of samples Nerium oleander 1 Olea europaea 2 Pilea microphylla 1 Prunus avium 4 Prunus domestica 1 Prunus persica 2 Prunus sp. 3 Quercus 1 Ranunculus repens 2 Rhus 1 Rosa 1 Rosmarinus 3 Rubus fruticosus 3 Rubus idaeus 2 Salix 5 Sambucus 3 Solanum lycopersicum 7 Trifolium repens 2 Ulmus 4 Urtica 1 Vitis 3 Xylella fastidiosa in Germany, information PAFF,

5 Surveillance Survey on vectors in 100m radius Sampling of vectors (with sweeping nets) and species determination in the laboratory of Saxony until 13 July vector samples taken: 1 Cercopis vulnerata (23 June) captured 1 Philaenus spumarius (11 July) captured Testing of captured potential vectors will be done at the Julius Kühn-Institute (at least for 2016) Xylella fastidiosa in Germany, information PAFF,

6 Survey on vectors 100m radius Xylella fastidiosanet sweeping for presence of vectors (in Pausa; Saxony) Results 23 June: 1 Cercopis vulnerata 11 July: 1 Philaenus spumarius Xylella fastidiosa in Germany, information PAFF,

7 Since 16 June Information campaign Letter to all owners in the 100m radius Information to mayor of Pausa Press release is in preparation Trace back of origin of the oleander plant Official interview of the owner Cutting was taken 4-6 years ago Cutting is not a souvenir from a journey No further information could be achieved Xylella fastidiosa in Germany, information PAFF,

8 Determination of X. fastidiosa subspecies Carried out by Julius Kühn-Institute 01 July: Preliminary result X. f. subsp. fastidiosa detected X. f. subsp. multiplex and X.f. subsp. sandyi not detected 12 July: Final result X. f. subsp. fastidiosa determined (preliminary result confirmed) Xylella fastidiosa in Germany, information PAFF,

9 Phylogenetic Tree of X. fastidiosa subspecies BioNJ BioNJ phylogenetic tree based on Maximum Likelihood distance analysis of the 2-isopropylmalate synthase (leua) gene as one of five gene regions used in Multi-Locus Sequence Type Analysis (Yuan et al. 2010) Xylella fastidiosa in Germany, information PAFF,

10 Natural infected host plants susceptible to X. f. subsp. fastidiosa according to EFSA updated database (EFSA Journal 2016; 14 (2):4378) name Name Name name Acer Diplocyclos Metrosideros Prunus persica palmatus Ambrosia artemisiifolia Humulus scandens Morus rubra Sambucus canadensis Ampelopsis brevipedunculata Lupinus aridorum Nerium oleander* Spartium junceum Cercis occidentalis Magnolia Pluchea odorata Vitis grandiflora Citrus Mallotus Prunus avium paniculatus Coffea arabica Medicago sativa Prunus dulcis *Nerium oleander - not reported before Up to now only Nerium oleander can be considered as host plant in the sense of Article 1 (b) of Implementing Decision (EU) 2015/789 Xylella fastidiosa in Germany, information PAFF,

11 7 July: Establishment of a demarcated area General order is adopted, will be published soon Infected zone Including infected plant and all plants near by, which may be infected Covering the whole plot area of the plant owner Buffer zone Buffer zone is of a width of 10 km, surrounding the infected zone 40 % of the area located in the Federal State of Thuringia Responsible authority of Thuringia is informed and will establish a demarcated area (buffer zone) as well Xylella fastidiosa in Germany, information PAFF,

12 Demarcated area - Infected zone + radius of 100 m Xylella fastidiosa in Germany, information PAFF,

13 Demarcated area - Buffer zone 10 km TH Violett line border between Saxony (SN) and Thuringia (TH) SN Xylella fastidiosa in Germany, information PAFF,

14 Buffer Zone (extract) split into 100 m 100 m squares Forest Green areas conifers Blue areas broad leaf Arable land Areas with yellow frame Xylella fastidiosa in Germany, information PAFF,

15 Ongoing activities Measures for elimination of host plants in 100 m area initiated Extension of the personal and technical capacity for the surveillance and the laboratory work in Saxony 700 plant samples/year 200 vector samples (net sweeping) Close co-operation between Saxony and Thuringia Support by the JKI in the diagnostic activities and technical advice Raising public awareness Xylella fastidiosa in Germany, information PAFF,

16 Thank you for your attention Xylella fastidiosa in Germany, information PAFF,

Xylella fastidiosa. Funded by the EU s LIFE programme

Xylella fastidiosa. Funded by the EU s LIFE programme Xylella fastidiosa Xylem-inhabiting fastidious bacteria Introduction The bacteria multiplies in the vessels and these become blocked, and water can not reach all parts of the plant from the roots and infected